Status
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Gene information
General information
TC033300
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015370,TC015371
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 38786..46351
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033300
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05806

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    leg musculature decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: The penetrance of the leg phenotype is unsure.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Morphologically normal but not hatch.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033300 RA
      MFYSLWVLL FITFGSAYD YRHALKLSL LFYEAQRSG 
      PLPASNRIT WRSDSALDD HGLNNEDLT GGYYDASDY 
      VKFSFTMAF TTTILTWGM LSFRHAYNE AGQYQHGLD 
      AIKWATDYF IKCHASKYE FYGQIGDFS LDHAFWGRP 
      EEMNMTRPA YKIDIEHPG SDLAGEAVA ALAAASLLF 
      ENFNQSYSD ELLRHATEL YDFATMYRG LYHDSIPGA 
      KVYYESTGY GDELTWAAI WLYKKTKDV KYIEQAETF 
      YTKFRIKDR PNEFFYNKK VAGIQLLLA ELTLRPEYI 
      STIKNFCDY TIKEQIRTP KGLIFIDKS GTLSHAANV 
      AFICLHAGL TLNISQAAY VSFAKEQIN YMLGSTGQS 
      FVVGYGQNY PKQPHHSAS SCPNLPEPC GWKQFTWKG 
      PNPQILYGA LVSGPDQND HYEDVREEF LYNEVTLDY 
      NAGFQSTLA GLIYIENEN KLTMACNCP VTQPGPTLA 
      STCGGAQYM LFMGLLEVF LRSQCDLED PCGRPHNTP 
      VLPEYDFII VGGGSSGAV VASRLSEIP EWNVLLIEA 
      GLDEPTGTQ VPSMFLNFI GSEIDWGYQ TEPEPSACL 
      AETEQRCYW PRGKVLGGT SVMNGMMYI RGSRKDYDD 
      WAKAGNEGW SYNEVLPYF LKSEDNKQA DSMDRGYHS 
      TGGLLTVSQ FPYHPPLSQ ALLKAAQEL GYPIRDLNG 
      AYHTGFNIA QTTNRNGSR LSTAKAFLR PFKNRRNLN 
      ILMNSTVTR VLINTTTKQ AYGVEVINN GVKQVIYAS 
      KEVIVSGGA INSPQILLL SGIGPSQDL QQVNVPVVH 
      NLPGVGKNL QNHVAHFVN FNINDTNSA PLNWATAME 
      YLLFRDGLM SGTGISEVT GFINTKYND PRLEHPDIQ 
      LFFGGFLAN CARTGQVGE RVDNGTRQI QMIPTVLHP 
      KSRGVLKLR DNNPLSTPL IYANYFTHP NDVKVITEG 
      IKFAMKLSE TKALKRYGF QLDRTPVQG CESLTFGTD 
      PYWDCAVKR QTGPENHQA GSCKMGPSS DPMAVVNPM 
      LQVHGIDRL RVIDASIMP AVTTGNTNA PCIMIAEKG 
      SDLIKSRWL TPQAGFFYT NMPNQRIDR QWGSW
    CDS sequence
    • >TC033300 RA
      atgttctac tcattgtgg gtgctacta tttattaca 
      tttggctcc gcctatgat tatcgccac gccctcaaa 
      ctctccctc cttttctac gaagcacaa cgttccggc 
      cctctccct gcctctaat cgcatcacg tggcgttca 
      gactccgcc cttgacgac catggtctt aacaacgaa 
      gacttgacc ggtggttac tacgacgcc agcgattac 
      gtcaaattt tcatttacg atggctttc acaaccacg 
      attttaaca tgggggatg ttatcattc cgacacgcc 
      tataacgaa gccggccaa tatcaacac ggtctcgat 
      gccatcaag tgggccacg gactatttt attaaatgc 
      cacgcatcc aagtacgag ttttacggc cagattggt 
      gatttttcg cttgaccac gccttctgg gggcggccc 
      gaagagatg aatatgaca cgaccggcc tataagatt 
      gacatagag catccaggg tcggattta gcgggagag 
      gcggtggcg gcattggca gccgctagt cttttattt 
      gaaaatttc aatcaatca tattcagac gaattgtta 
      cgccacgcg actgaattg tacgatttt gcgacaatg 
      tatcgaggg ttgtatcac gattctata ccaggggct 
      aaggtctat tacgagtct acagggtac ggagatgaa 
      ctaacgtgg gctgccatt tggttgtat aaaaaaacc 
      aaagatgtg aaatatatc gaacaagcc gaaacgttt 
      tacacgaaa tttcggatt aaagacaga ccgaacgaa 
      tttttctat aataaaaaa gtggccggc attcagctt 
      cttttagct gaattaaca ctccgccca gaatacatc 
      tcgacaata aaaaatttc tgcgattat acaatcaaa 
      gaacagatc cggactccc aagggcctt attttcatc 
      gacaagtca gggacacta tcacatgcc gctaatgtc 
      gcattcatt tgcctacat gccggtctt actttaaac 
      atatcgcag gcggcttat gtcagtttt gccaaagaa 
      caaatcaac tacatgtta ggctcaacg ggtcaaagt 
      tttgtagtt ggctatggc caaaattac cctaaacag 
      ccgcatcat tcagccagt tcctgtccg aatttgccc 
      gaaccttgc gggtggaaa caatttacg tggaaaggg 
      ccgaatcca caaattttg tatggggcc cttgtaagt 
      ggccccgat caaaatgat cattacgaa gacgtaaga 
      gaggaattt ttgtataat gaagtgaca ctggattac 
      aatgcggga tttcaaagc actctagct ggacttatt 
      tatattgaa aatgagaat aaattaacg atggcttgc 
      aattgtccc gtgacgcaa ccaggcccc accctcgcc 
      tccacctgt ggtggtgcc caatatatg cttttcatg 
      ggtttattg gaggtgttt ttgaggtca caatgtgac 
      cttgaagac ccatgtggc cgaccccat aacaccccc 
      gttctccct gaatacgat tttattatt gtcgggggt 
      ggcagttcg ggggcggtg gtggcaagt cgtctctcg 
      gaaattccc gaatggaat gtactttta atcgaagca 
      ggcttggac gaaccaacc ggtacccaa gtaccgtca 
      atgttttta aattttatt ggttcggaa attgactgg 
      gggtatcag acggaacca gaaccttca gcgtgtctg 
      gcagagacg gaacagagg tgctattgg ccaagaggg 
      aaagtactg ggtggtact tcggtcatg aatgggatg 
      atgtatatc cgagggtcg agaaaggat tacgacgat 
      tgggcaaaa gctggcaat gaaggatgg agttataat 
      gaagtacta ccctatttt ctaaagagt gaagataat 
      aagcaagcg gatagtatg gataggggg taccattca 
      acagggggg ttgttgaca gtgtcacag tttccctat 
      catccacca ctatcacag gcgttgcta aaggcggcg 
      caagagttg gggtaccca atcagggac ttgaatgga 
      gcgtatcat actgggttt aacattgcg caaacaacc 
      aatagaaat gggtcgagg ttaagtacg gctaaagct 
      tttctaagg ccgttcaaa aatcgaaga aacttgaat 
      attttgatg aactcaacc gtaaccaga gttctcatc 
      aatacaacc acgaaacag gcatatgga gtcgaagtt 
      attaataat ggcgtcaaa caagtaatt tacgcgagt 
      aaagaggtt atagtatct gggggtgct atcaactcg 
      ccccaaatc ctactttta agtggcata ggtcccagt 
      caggacctc caacaggtc aacgttcct gtggtgcac 
      aatctccca ggtgtgggc aaaaatctt caaaatcat 
      gtcgcacat tttgtcaat ttcaatatc aatgataca 
      aatagtgca cctttgaac tgggccaca gctatggag 
      taccttctt ttccgtgac ggtctaatg agtggtacc 
      ggtatatcc gaagtgact ggattcata aacacgaaa 
      tataacgat ccaagactg gagcacccc gatattcag 
      ttgttcttt ggaggattt ttagcaaat tgtgcaagg 
      accggacaa gtgggggag cgggtggat aatggtact 
      cgacaaatt caaatgata ccaactgtc ttgcaccca 
      aaaagtcgc ggagtatta aaattgaga gataacaac 
      cctctcagc acacctttg atttatgcc aattatttc 
      acacatccg aatgatgtc aaggttatc actgaaggg 
      atcaaattt gcaatgaaa ctatcggaa actaaagct 
      ttgaaacga tacggattc caacttgac cggacaccg 
      gtccagggc tgtgaatcc cttactttt ggtacagat 
      ccgtattgg gattgtgcc gtaaaacga caaaccggt 
      ccagagaac catcaagcc ggtagttgt aaaatgggt 
      ccatccagt gaccccatg gctgttgtt aacccaatg 
      ctccaggtc cacggtatt gaccggtta cgggttatt 
      gatgcaagt attatgcca gcggttaca accggaaac 
      accaacgct ccctgtatc atgatagcg gaaaaaggg 
      agcgatttg atcaaatca aggtggcta acaccacaa 
      gctgggttc ttttacaca aatatgccc aaccaaagg 
      atagacagg caatggggg tcctggtga
    mRNA sequence
    • >TC033300 RA
      caaaagtga aatacattt accaactta actttccgt 
      gactcaacc actcatcca accccaaaa tgtaaactc 
      ccttaaaaa aaacacacg atgttctac tcattgtgg 
      gtgctacta tttattaca tttggctcc gcctatgat 
      tatcgccac gccctcaaa ctctccctc cttttctac 
      gaagcacaa cgttccggc cctctccct gcctctaat 
      cgcatcacg tggcgttca gactccgcc cttgacgac 
      catggtctt aacaacgaa gacttgacc ggtggttac 
      tacgacgcc agcgattac gtcaaattt tcatttacg 
      atggctttc acaaccacg attttaaca tgggggatg 
      ttatcattc cgacacgcc tataacgaa gccggccaa 
      tatcaacac ggtctcgat gccatcaag tgggccacg 
      gactatttt attaaatgc cacgcatcc aagtacgag 
      ttttacggc cagattggt gatttttcg cttgaccac 
      gccttctgg gggcggccc gaagagatg aatatgaca 
      cgaccggcc tataagatt gacatagag catccaggg 
      tcggattta gcgggagag gcggtggcg gcattggca 
      gccgctagt cttttattt gaaaatttc aatcaatca 
      tattcagac gaattgtta cgccacgcg actgaattg 
      tacgatttt gcgacaatg tatcgaggg ttgtatcac 
      gattctata ccaggggct aaggtctat tacgagtct 
      acagggtac ggagatgaa ctaacgtgg gctgccatt 
      tggttgtat aaaaaaacc aaagatgtg aaatatatc 
      gaacaagcc gaaacgttt tacacgaaa tttcggatt 
      aaagacaga ccgaacgaa tttttctat aataaaaaa 
      gtggccggc attcagctt cttttagct gaattaaca 
      ctccgccca gaatacatc tcgacaata aaaaatttc 
      tgcgattat acaatcaaa gaacagatc cggactccc 
      aagggcctt attttcatc gacaagtca gggacacta 
      tcacatgcc gctaatgtc gcattcatt tgcctacat 
      gccggtctt actttaaac atatcgcag gcggcttat 
      gtcagtttt gccaaagaa caaatcaac tacatgtta 
      ggctcaacg ggtcaaagt tttgtagtt ggctatggc 
      caaaattac cctaaacag ccgcatcat tcagccagt 
      tcctgtccg aatttgccc gaaccttgc gggtggaaa 
      caatttacg tggaaaggg ccgaatcca caaattttg 
      tatggggcc cttgtaagt ggccccgat caaaatgat 
      cattacgaa gacgtaaga gaggaattt ttgtataat 
      gaagtgaca ctggattac aatgcggga tttcaaagc 
      actctagct ggacttatt tatattgaa aatgagaat 
      aaattaacg atggcttgc aattgtccc gtgacgcaa 
      ccaggcccc accctcgcc tccacctgt ggtggtgcc 
      caatatatg cttttcatg ggtttattg gaggtgttt 
      ttgaggtca caatgtgac cttgaagac ccatgtggc 
      cgaccccat aacaccccc gttctccct gaatacgat 
      tttattatt gtcgggggt ggcagttcg ggggcggtg 
      gtggcaagt cgtctctcg gaaattccc gaatggaat 
      gtactttta atcgaagca ggcttggac gaaccaacc 
      ggtacccaa gtaccgtca atgttttta aattttatt 
      ggttcggaa attgactgg gggtatcag acggaacca 
      gaaccttca gcgtgtctg gcagagacg gaacagagg 
      tgctattgg ccaagaggg aaagtactg ggtggtact 
      tcggtcatg aatgggatg atgtatatc cgagggtcg 
      agaaaggat tacgacgat tgggcaaaa gctggcaat 
      gaaggatgg agttataat gaagtacta ccctatttt 
      ctaaagagt gaagataat aagcaagcg gatagtatg 
      gataggggg taccattca acagggggg ttgttgaca 
      gtgtcacag tttccctat catccacca ctatcacag 
      gcgttgcta aaggcggcg caagagttg gggtaccca 
      atcagggac ttgaatgga gcgtatcat actgggttt 
      aacattgcg caaacaacc aatagaaat gggtcgagg 
      ttaagtacg gctaaagct tttctaagg ccgttcaaa 
      aatcgaaga aacttgaat attttgatg aactcaacc 
      gtaaccaga gttctcatc aatacaacc acgaaacag 
      gcatatgga gtcgaagtt attaataat ggcgtcaaa 
      caagtaatt tacgcgagt aaagaggtt atagtatct 
      gggggtgct atcaactcg ccccaaatc ctactttta 
      agtggcata ggtcccagt caggacctc caacaggtc 
      aacgttcct gtggtgcac aatctccca ggtgtgggc 
      aaaaatctt caaaatcat gtcgcacat tttgtcaat 
      ttcaatatc aatgataca aatagtgca cctttgaac 
      tgggccaca gctatggag taccttctt ttccgtgac 
      ggtctaatg agtggtacc ggtatatcc gaagtgact 
      ggattcata aacacgaaa tataacgat ccaagactg 
      gagcacccc gatattcag ttgttcttt ggaggattt 
      ttagcaaat tgtgcaagg accggacaa gtgggggag 
      cgggtggat aatggtact cgacaaatt caaatgata 
      ccaactgtc ttgcaccca aaaagtcgc ggagtatta 
      aaattgaga gataacaac cctctcagc acacctttg 
      atttatgcc aattatttc acacatccg aatgatgtc 
      aaggttatc actgaaggg atcaaattt gcaatgaaa 
      ctatcggaa actaaagct ttgaaacga tacggattc 
      caacttgac cggacaccg gtccagggc tgtgaatcc 
      cttactttt ggtacagat ccgtattgg gattgtgcc 
      gtaaaacga caaaccggt ccagagaac catcaagcc 
      ggtagttgt aaaatgggt ccatccagt gaccccatg 
      gctgttgtt aacccaatg ctccaggtc cacggtatt 
      gaccggtta cgggttatt gatgcaagt attatgcca 
      gcggttaca accggaaac accaacgct ccctgtatc 
      atgatagcg gaaaaaggg agcgatttg atcaaatca 
      aggtggcta acaccacaa gctgggttc ttttacaca 
      aatatgccc aaccaaagg atagacagg caatggggg 
      tcctggtga tggggtagt gcaattgta ggtttactt 
      gtatttatt ttctgtaga ttgtgaata aatttttaa 
      acagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY