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Gene information
General information
TC015356 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 66174..67294
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015356
      iBeetle screen iB_05803

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larva anterior decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015356 RA
        MPPIASLLL LLTISQVDL TSTQDLKTI FRTPNKRNY 
        DEFEILERS NFVSENSES KNYPNEKPK SYANAQILL 
        RIGGDKEGC ACGNNCKCC GGRNYPFYP LCLCLPPCY 
        PYLPPLYAQ PMKDVPPLL PPPVKAELP PPPKLAENE 
        LNENVVDEI EENVRNEHP VILDTIKIP DDAIKPVDD 
        IEKCLKDVS SKIDPKQVS PNVKFVSLD LSQVPTKNP 
        DNDKRDLQE SLIPYLGSG QIRPKLLVT HPIFNRPLS 
        ESVGYKFSE GSWYYSDPV KSVAYQKPP ILTVLGPQQ 
        VVKYLVVPV TEPEVVPRS HDEDCCCCS CAKYVGELN 
        EKMA
      • >TC015356 RB
        MPPIASLLL LLTISQNRK ITPTKNQNH TQMHKFCSE 
        LVEIRRAVL AVTIANVVE EEITPFILC VCVCRLVTP 
        TYPHSMLNQ
      CDS sequence
      • >TC015356 RA
        atgccacca atcgcatca cttctcttg ttactcaca 
        atctctcag gtcgattta acgtctacc caagacctt 
        aaaacaatt tttcgcact ccaaacaag cgtaattac 
        gacgaattc gaaattctt gaacgctca aattttgta 
        tcggaaaat tcagaatcg aaaaattac cccaacgaa 
        aaaccaaaa tcatacgca aatgcacaa attctgctc 
        cgaattggt ggagataag gagggctgt gcttgcggt 
        aacaattgc aaatgttgt ggaggaaga aattacccc 
        ttttatcct ttgtgtttg tgtttgccg ccttgttac 
        ccctactta cccccactc tatgctcaa ccaatgaaa 
        gatgttccc ccactactg ccaccgcca gtcaaagcg 
        gagcttccc ccaccacca aaattggcc gaaaatgaa 
        ttgaacgaa aacgtggtt gatgaaata gaagaaaat 
        gtcagaaat gaacatcct gtcattttg gatactatc 
        aaaattcct gatgatgcc attaaacca gtggacgac 
        attgaaaaa tgtctcaag gacgtgtcc tcgaaaatt 
        gatcccaaa caagtgtca ccaaacgtt aagttcgtg 
        tctttggat ttgagtcaa gtaccgaca aaaaatccg 
        gataatgac aagcgtgac cttcaagag tcacttatt 
        ccctacttg ggaagtggc caaatcagg ccgaaactt 
        ttagtgaca catcctatt tttaatagg cctttaagt 
        gaatcagtt ggatataaa ttttcagaa ggttcttgg 
        tactattca gatccggtg aaaagtgta gcatatcaa 
        aaaccacca attttgaca gttttgggg ccccaacaa 
        gtggtcaag tatttagtg gtaccagtg actgaacct 
        gaagtggta ccaaggtcg catgatgag gattgttgt 
        tgctgcagt tgtgcgaaa tatgtggga gaattgaac 
        gaaaaaatg gcctaa
      • >TC015356 RB
        atgccacca atcgcatca cttctcttg ttactcaca 
        atctctcag aatcgaaaa attacccca acgaaaaac 
        caaaatcat acgcaaatg cacaaattc tgctccgaa 
        ttggtggag ataaggagg gctgtgctt gcggtaaca 
        attgcaaat gttgtggag gaagaaatt acccctttt 
        atcctttgt gtttgtgtt tgccgcctt gttacccct 
        acttacccc cactctatg ctcaaccaa tga
      mRNA sequence
      • >TC015356 RA
        aaaacagtc acaaaaatg ccaccaatc gcatcactt 
        ctcttgtta ctcacaatc tctcaggtc gatttaacg 
        tctacccaa gaccttaaa acaattttt cgcactcca 
        aacaagcgt aattacgac gaattcgaa attcttgaa 
        cgctcaaat tttgtatcg gaaaattca gaatcgaaa 
        aattacccc aacgaaaaa ccaaaatca tacgcaaat 
        gcacaaatt ctgctccga attggtgga gataaggag 
        ggctgtgct tgcggtaac aattgcaaa tgttgtgga 
        ggaagaaat taccccttt tatcctttg tgtttgtgt 
        ttgccgcct tgttacccc tacttaccc ccactctat 
        gctcaacca atgaaagat gttccccca ctactgcca 
        ccgccagtc aaagcggag cttccccca ccaccaaaa 
        ttggccgaa aatgaattg aacgaaaac gtggttgat 
        gaaatagaa gaaaatgtc agaaatgaa catcctgtc 
        attttggat actatcaaa attcctgat gatgccatt 
        aaaccagtg gacgacatt gaaaaatgt ctcaaggac 
        gtgtcctcg aaaattgat cccaaacaa gtgtcacca 
        aacgttaag ttcgtgtct ttggatttg agtcaagta 
        ccgacaaaa aatccggat aatgacaag cgtgacctt 
        caagagtca cttattccc tacttggga agtggccaa 
        atcaggccg aaactttta gtgacacat cctattttt 
        aataggcct ttaagtgaa tcagttgga tataaattt 
        tcagaaggt tcttggtac tattcagat ccggtgaaa 
        agtgtagca tatcaaaaa ccaccaatt ttgacagtt 
        ttggggccc caacaagtg gtcaagtat ttagtggta 
        ccagtgact gaacctgaa gtggtacca aggtcgcat 
        gatgaggat tgttgttgc tgcagttgt gcgaaatat 
        gtgggagaa ttgaacgaa aaaatggcc taattctta 
        ttagcacta tttatttat tgtagtaaa attacactt 
        ttggtgcaa atatatttg ttacattgg t
      • >TC015356 RB
        aaaacagtc acaaaaatg ccaccaatc gcatcactt 
        ctcttgtta ctcacaatc tctcagaat cgaaaaatt 
        accccaacg aaaaaccaa aatcatacg caaatgcac 
        aaattctgc tccgaattg gtggagata aggagggct 
        gtgcttgcg gtaacaatt gcaaatgtt gtggaggaa 
        gaaattacc ccttttatc ctttgtgtt tgtgtttgc 
        cgccttgtt acccctact tacccccac tctatgctc 
        aaccaatga aagatgttc ccccactac tgccaccgc 
        cagtcaaag cggagcttc ccccaccac caaaattgg 
        ccgaaaatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY