| General information | |||
| TC015354 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 76471..85094 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015354 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05802 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015354 RA
MDALSADLA LQAILGRGS DFVSGIDNE ALDFSQLED FINSEGSQS TNPYFNESL NSTPPSGKL LNVIVENKR NAGHSLPES PPDSSSEHP YSPQEGCEP LNPSENIYT TLGPNIYKP VIGPMLGDN LILGSHIVV AEQNGIMQD TGLMIPEDS NQNFTDRGI YKGDENLVH LGYTPAIEN NLCEPNLDN IQTVYTNLQ SAPKKRKLS QDSPLVKSE PENCTTSQL SPPGPLSTP LDEEYASSE ACLSESQYQ CIRFSPFQQ PQWHALCDQ SLTELPVPH YRVDADKGF NFSNADDAF VCQKKNHFQ ITCHVQLLG DAQFVKTPD GFQKISSFH LHFYGVKHD CPTQTIRVE QSQSDRSKK PFHPVLVEL VNSQVTKVT VGRLHFSET TSNNMRKKG KPNPEQRYF QLVVGLHAH TTNGNFPVI SHASQKIIV RASNPGQFE SDVELCWQK GQTQDSIFH NGKVGINTD RPDESLVVH GNIKVTGHI IQPSDIRAK KNIVECDTA EQLRNVQKL RVVRYDYEP SFASQLSRD SQHSDTGVI AQEVAKILP EAVRPAGDL VLKNGQSID NFLVVNKER IFMENIGAV KELCKVTDN LETRIDQLE RINRRLNKL KRGDSLKST STVSSLKHS NSMQKCSKH HCDRDDELC SNKFIQIII VILVLIMAF CLAAITTLY LMEASRHNQ FVRTNLQQT HRSSIVTKR QMKVPKNHQ SRHTEYHKT SNNNYKDDD VTRITIDPS LTKRPSAII GRPPDCLSV DQDLESSHL CQIYCCAAT QPPVIPPPE SPNSIYGDK STNRTVLNL EQAKLATPF HSTTNEIDK RQKRDAYDL TGEISSFDM PDDQIFLII VQGRNFNTT LTHKYLIAQ PEPHNYSYS VPISKYMPD EFINLIFRS TQESIKEVE YCNNNYKER GCAFEKPQT RHKVNEHSP SQLISTQSV IFEVNMADF QSKIMTYRA SSRANQNTC KLSNLDEFI EYNFHFYRH CDE
- >TC015354 RA
atggacgca ttatcggcc gatttggcg ctacaggcg attttaggt cgcggttcg gactttgtc agcggcatc gataacgaa gcccttgac ttttcccaa ttggaagac ttcataaac agcgaaggc tcccaaagt acgaaccct tatttcaac gaatcgcta aattcgacg ccaccttca ggtaaacta ctgaatgtt attgtggaa aacaagcga aatgcgggc cattctttg cccgaaagc cctcctgat agcagttca gagcaccct tatagcccc caagagggt tgcgagcca cttaacccc tctgagaat atttacacc actttgggg ccaaatatc tacaaacca gttattggc cccatgttg ggcgacaat ttaattttg ggctcacac atcgtagta gccgagcaa aatgggatc atgcaagat acgggattg atgatccct gaggattcg aatcagaat tttacagat agagggata tataaaggg gatgagaat ttggtacat ttgggctat accccggct attgaaaat aacttgtgt gaaccgaat ttggataac atacaaacg gtttatacg aatttacaa agtgccccc aaaaaacga aaattatcg caagattcg cctttggtc aaaagtgag ccggaaaat tgcacgacg agtcaattg agccctcct gggcctctt tccacaccg ttggatgaa gagtacgca tcttctgaa gcttgcctt tctgaatct caatatcaa tgtattaga tttagccct tttcaacag ccacaatgg cacgcactc tgcgatcaa agtttgacc gaattgccg gtaccacat tatcgagtt gatgccgat aaaggcttc aatttttca aacgccgac gatgccttt gtatgccag aaaaagaac catttccag ataacttgc cacgtccaa ttattgggt gacgcacaa tttgttaaa actcccgac ggtttccaa aagatctca agcttccat ctacacttt tacggtgtt aaacatgac tgtccgact caaacgata cgagttgag caaagtcaa agtgataga agcaaaaaa ccatttcat cccgttttg gtcgaactt gtgaattca caagtaaca aaggtcaca gtaggccga ttacatttc agtgaaaca acgtcgaat aacatgcga aaaaagggt aaaccgaat cctgaacag cgatatttt cagttggtt gtaggtcta catgcacat actacaaat ggtaatttt ccggttata agtcacgcg agtcaaaaa ataattgtg agggcttca aatcctggc caatttgaa agtgacgtt gaattgtgt tggcaaaaa ggccagacg caagactca atttttcat aatgggaaa gttggtatt aatacggac agaccggac gaatcattg gtcgtccat ggtaatatt aaagtaact ggacatatt atacaacca agtgacata agggcgaag aaaaatatc gtcgaatgt gacacggct gagcagcta cgaaacgtc caaaaactc agagttgtt aggtatgac tacgaaccg tcgtttgca tctcagtta agtcgtgat tcacaacac tcggatacc ggtgtgata gctcaggaa gtggctaaa atcctcccg gaagcggtc agaccggct ggtgacctt gtcttaaag aatggtcaa tccattgat aattttttg gtagtcaac aaggagcgc atttttatg gaaaatatc ggtgctgtt aaggaatta tgtaaagtg actgataat ttggagact cgaatcgat caattagaa cgaatcaat cgccgattg aataaattg aaacgtggt gatagtctc aaatcgact agtacagtg agtagcttg aaacattcc aattcaatg caaaaatgt tccaaacat cattgtgac cgtgacgac gaactctgt tcgaataaa tttattcaa ataattatc gtcattctg gttcttata atggcattt tgtttggcc gctataaca accttgtac ctgatggaa gcctcacga cacaaccaa ttcgtccga actaacctc caacaaacc catcggtcg tccatagta accaaacga caaatgaaa gtgccgaaa aaccatcaa tcacgacac accgaatac cacaaaaca agtaacaat aattacaaa gacgatgac gtcacacgt atcaccata gatccgagt ttaacgaaa cggccttcg gctatcatc ggccgacca cccgattgt ctatcggtt gatcaagac cttgaaagt agccatctt tgtcagatt tattgttgc gcagcaaca caaccaccg gtaattccc ccaccggaa tcgcccaat tcaatttat ggcgataaa tcaacgaat cgtacagtc ctaaatcta gaacaagcg aaattagcc acgccattt cactcgaca accaatgag attgacaaa cgacaaaaa cgagacgct tatgatttg acaggcgag ataagttcg tttgacatg ccagatgac cagattttc ttgattatt gttcaaggt cgtaacttt aacacgacc ttgacgcat aaatatcta atagctcag cctgagccg cataattat tcctattcc gttcctatt tcgaaatat atgcccgac gagttcatt aatctcata ttcagatca actcaagaa tcgatcaag gaggtcgag tattgcaat aataactat aaggaacgt ggatgtgcc tttgagaag ccgcaaaca agacataaa gtcaatgag cacagtccg agtcagttg attagtaca caaagtgtc atttttgag gtcaatatg gccgatttt cagtcgaaa attatgacg taccgagcg tcgagtcga gccaatcag aatacgtgt aaattgagc aacttggat gaatttatc gaatataat ttccatttc taccggcac tgtgatgaa taa
- >TC015354 RA
acctccttt aacttgcat tgaaataga aaatcgttc aaaattcat tcaagtgat gcgaaaaaa aaacgtgaa aatgattga gttttgatg tgatatttt gcaagaaat ggacgcatt atcggccga tttggcgct acaggcgat tttaggtcg cggttcgga ctttgtcag cggcatcga taacgaagc ccttgactt ttcccaatt ggaagactt cataaacag cgaaggctc ccaaagtac gaaccctta tttcaacga atcgctaaa ttcgacgcc accttcagg taaactact gaatgttat tgtggaaaa caagcgaaa tgcgggcca ttctttgcc cgaaagccc tcctgatag cagttcaga gcaccctta tagccccca agagggttg cgagccact taacccctc tgagaatat ttacaccac tttggggcc aaatatcta caaaccagt tattggccc catgttggg cgacaattt aattttggg ctcacacat cgtagtagc cgagcaaaa tgggatcat gcaagatac gggattgat gatccctga ggattcgaa tcagaattt tacagatag agggatata taaagggga tgagaattt ggtacattt gggctatac cccggctat tgaaaataa cttgtgtga accgaattt ggataacat acaaacggt ttatacgaa tttacaaag tgcccccaa aaaacgaaa attatcgca agattcgcc tttggtcaa aagtgagcc ggaaaattg cacgacgag tcaattgag ccctcctgg gcctctttc cacaccgtt ggatgaaga gtacgcatc ttctgaagc ttgcctttc tgaatctca atatcaatg tattagatt tagcccttt tcaacagcc acaatggca cgcactctg cgatcaaag tttgaccga attgccggt accacatta tcgagttga tgccgataa aggcttcaa tttttcaaa cgccgacga tgcctttgt atgccagaa aaagaacca tttccagat aacttgcca cgtccaatt attgggtga cgcacaatt tgttaaaac tcccgacgg tttccaaaa gatctcaag cttccatct acactttta cggtgttaa acatgactg tccgactca aacgatacg agttgagca aagtcaaag tgatagaag caaaaaacc atttcatcc cgttttggt cgaacttgt gaattcaca agtaacaaa ggtcacagt aggccgatt acatttcag tgaaacaac gtcgaataa catgcgaaa aaagggtaa accgaatcc tgaacagcg atattttca gttggttgt aggtctaca tgcacatac tacaaatgg taattttcc ggttataag tcacgcgag tcaaaaaat aattgtgag ggcttcaaa tcctggcca atttgaaag tgacgttga attgtgttg gcaaaaagg ccagacgca agactcaat ttttcataa tgggaaagt tggtattaa tacggacag accggacga atcattggt cgtccatgg taatattaa agtaactgg acatattat acaaccaag tgacataag ggcgaagaa aaatatcgt cgaatgtga cacggctga gcagctacg aaacgtcca aaaactcag agttgttag gtatgacta cgaaccgtc gtttgcatc tcagttaag tcgtgattc acaacactc ggataccgg tgtgatagc tcaggaagt ggctaaaat cctcccgga agcggtcag accggctgg tgaccttgt cttaaagaa tggtcaatc cattgataa ttttttggt agtcaacaa ggagcgcat ttttatgga aaatatcgg tgctgttaa ggaattatg taaagtgac tgataattt ggagactcg aatcgatca attagaacg aatcaatcg ccgattgaa taaattgaa acgtggtga tagtctcaa atcgactag tacagtgag tagcttgaa acattccaa ttcaatgca aaaatgttc caaacatca ttgtgaccg tgacgacga actctgttc gaataaatt tattcaaat aattatcgt cattctggt tcttataat ggcattttg tttggccgc tataacaac cttgtacct gatggaagc ctcacgaca caaccaatt cgtccgaac taacctcca acaaaccca tcggtcgtc catagtaac caaacgaca aatgaaagt gccgaaaaa ccatcaatc acgacacac cgaatacca caaaacaag taacaataa ttacaaaga cgatgacgt cacacgtat caccataga tccgagttt aacgaaacg gccttcggc tatcatcgg ccgaccacc cgattgtct atcggttga tcaagacct tgaaagtag ccatctttg tcagattta ttgttgcgc agcaacaca accaccggt aattccccc accggaatc gcccaattc aatttatgg cgataaatc aacgaatcg tacagtcct aaatctaga acaagcgaa attagccac gccatttca ctcgacaac caatgagat tgacaaacg acaaaaacg agacgctta tgatttgac aggcgagat aagttcgtt tgacatgcc agatgacca gattttctt gattattgt tcaaggtcg taactttaa cacgacctt gacgcataa atatctaat agctcagcc tgagccgca taattattc ctattccgt tcctatttc gaaatatat gcccgacga gttcattaa tctcatatt cagatcaac tcaagaatc gatcaagga ggtcgagta ttgcaataa taactataa ggaacgtgg atgtgcctt tgagaagcc gcaaacaag acataaagt caatgagca cagtccgag tcagttgat tagtacaca aagtgtcat ttttgaggt caatatggc cgattttca gtcgaaaat tatgacgta ccgagcgtc gagtcgagc caatcagaa tacgtgtaa attgagcaa cttggatga atttatcga atataattt ccatttcta ccggcactg tgatgaata aacgatacg tactactca aaaaaagga aactaagaa caagaaatg catgacatt tatttattt acgtgtttt tttttaaat attatattt ttttaattg ttttatcaa agtgttcga tgttactac agcatcacc tagttgtag aagtacctg tatctgaaa gatttgaac acttttcta gttagtgta aaatgaaaa agcaccaaa aaaatattg tagcgtttt tagattatg actcatata atgtactta attttctcg tccgtccaa taaattgta taatgtgta gattagact tttatcgta ttgtaaaat atttttctt gtttgacaa atttttatt attttatag aattaaaaa tataaatga aca
