Status
Please wait ...
Gene information
General information
TC015354 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 76471..85094
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015354
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05802

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015354 RA
      MDALSADLA LQAILGRGS DFVSGIDNE ALDFSQLED 
      FINSEGSQS TNPYFNESL NSTPPSGKL LNVIVENKR 
      NAGHSLPES PPDSSSEHP YSPQEGCEP LNPSENIYT 
      TLGPNIYKP VIGPMLGDN LILGSHIVV AEQNGIMQD 
      TGLMIPEDS NQNFTDRGI YKGDENLVH LGYTPAIEN 
      NLCEPNLDN IQTVYTNLQ SAPKKRKLS QDSPLVKSE 
      PENCTTSQL SPPGPLSTP LDEEYASSE ACLSESQYQ 
      CIRFSPFQQ PQWHALCDQ SLTELPVPH YRVDADKGF 
      NFSNADDAF VCQKKNHFQ ITCHVQLLG DAQFVKTPD 
      GFQKISSFH LHFYGVKHD CPTQTIRVE QSQSDRSKK 
      PFHPVLVEL VNSQVTKVT VGRLHFSET TSNNMRKKG 
      KPNPEQRYF QLVVGLHAH TTNGNFPVI SHASQKIIV 
      RASNPGQFE SDVELCWQK GQTQDSIFH NGKVGINTD 
      RPDESLVVH GNIKVTGHI IQPSDIRAK KNIVECDTA 
      EQLRNVQKL RVVRYDYEP SFASQLSRD SQHSDTGVI 
      AQEVAKILP EAVRPAGDL VLKNGQSID NFLVVNKER 
      IFMENIGAV KELCKVTDN LETRIDQLE RINRRLNKL 
      KRGDSLKST STVSSLKHS NSMQKCSKH HCDRDDELC 
      SNKFIQIII VILVLIMAF CLAAITTLY LMEASRHNQ 
      FVRTNLQQT HRSSIVTKR QMKVPKNHQ SRHTEYHKT 
      SNNNYKDDD VTRITIDPS LTKRPSAII GRPPDCLSV 
      DQDLESSHL CQIYCCAAT QPPVIPPPE SPNSIYGDK 
      STNRTVLNL EQAKLATPF HSTTNEIDK RQKRDAYDL 
      TGEISSFDM PDDQIFLII VQGRNFNTT LTHKYLIAQ 
      PEPHNYSYS VPISKYMPD EFINLIFRS TQESIKEVE 
      YCNNNYKER GCAFEKPQT RHKVNEHSP SQLISTQSV 
      IFEVNMADF QSKIMTYRA SSRANQNTC KLSNLDEFI 
      EYNFHFYRH CDE
    CDS sequence
    • >TC015354 RA
      atggacgca ttatcggcc gatttggcg ctacaggcg 
      attttaggt cgcggttcg gactttgtc agcggcatc 
      gataacgaa gcccttgac ttttcccaa ttggaagac 
      ttcataaac agcgaaggc tcccaaagt acgaaccct 
      tatttcaac gaatcgcta aattcgacg ccaccttca 
      ggtaaacta ctgaatgtt attgtggaa aacaagcga 
      aatgcgggc cattctttg cccgaaagc cctcctgat 
      agcagttca gagcaccct tatagcccc caagagggt 
      tgcgagcca cttaacccc tctgagaat atttacacc 
      actttgggg ccaaatatc tacaaacca gttattggc 
      cccatgttg ggcgacaat ttaattttg ggctcacac 
      atcgtagta gccgagcaa aatgggatc atgcaagat 
      acgggattg atgatccct gaggattcg aatcagaat 
      tttacagat agagggata tataaaggg gatgagaat 
      ttggtacat ttgggctat accccggct attgaaaat 
      aacttgtgt gaaccgaat ttggataac atacaaacg 
      gtttatacg aatttacaa agtgccccc aaaaaacga 
      aaattatcg caagattcg cctttggtc aaaagtgag 
      ccggaaaat tgcacgacg agtcaattg agccctcct 
      gggcctctt tccacaccg ttggatgaa gagtacgca 
      tcttctgaa gcttgcctt tctgaatct caatatcaa 
      tgtattaga tttagccct tttcaacag ccacaatgg 
      cacgcactc tgcgatcaa agtttgacc gaattgccg 
      gtaccacat tatcgagtt gatgccgat aaaggcttc 
      aatttttca aacgccgac gatgccttt gtatgccag 
      aaaaagaac catttccag ataacttgc cacgtccaa 
      ttattgggt gacgcacaa tttgttaaa actcccgac 
      ggtttccaa aagatctca agcttccat ctacacttt 
      tacggtgtt aaacatgac tgtccgact caaacgata 
      cgagttgag caaagtcaa agtgataga agcaaaaaa 
      ccatttcat cccgttttg gtcgaactt gtgaattca 
      caagtaaca aaggtcaca gtaggccga ttacatttc 
      agtgaaaca acgtcgaat aacatgcga aaaaagggt 
      aaaccgaat cctgaacag cgatatttt cagttggtt 
      gtaggtcta catgcacat actacaaat ggtaatttt 
      ccggttata agtcacgcg agtcaaaaa ataattgtg 
      agggcttca aatcctggc caatttgaa agtgacgtt 
      gaattgtgt tggcaaaaa ggccagacg caagactca 
      atttttcat aatgggaaa gttggtatt aatacggac 
      agaccggac gaatcattg gtcgtccat ggtaatatt 
      aaagtaact ggacatatt atacaacca agtgacata 
      agggcgaag aaaaatatc gtcgaatgt gacacggct 
      gagcagcta cgaaacgtc caaaaactc agagttgtt 
      aggtatgac tacgaaccg tcgtttgca tctcagtta 
      agtcgtgat tcacaacac tcggatacc ggtgtgata 
      gctcaggaa gtggctaaa atcctcccg gaagcggtc 
      agaccggct ggtgacctt gtcttaaag aatggtcaa 
      tccattgat aattttttg gtagtcaac aaggagcgc 
      atttttatg gaaaatatc ggtgctgtt aaggaatta 
      tgtaaagtg actgataat ttggagact cgaatcgat 
      caattagaa cgaatcaat cgccgattg aataaattg 
      aaacgtggt gatagtctc aaatcgact agtacagtg 
      agtagcttg aaacattcc aattcaatg caaaaatgt 
      tccaaacat cattgtgac cgtgacgac gaactctgt 
      tcgaataaa tttattcaa ataattatc gtcattctg 
      gttcttata atggcattt tgtttggcc gctataaca 
      accttgtac ctgatggaa gcctcacga cacaaccaa 
      ttcgtccga actaacctc caacaaacc catcggtcg 
      tccatagta accaaacga caaatgaaa gtgccgaaa 
      aaccatcaa tcacgacac accgaatac cacaaaaca 
      agtaacaat aattacaaa gacgatgac gtcacacgt 
      atcaccata gatccgagt ttaacgaaa cggccttcg 
      gctatcatc ggccgacca cccgattgt ctatcggtt 
      gatcaagac cttgaaagt agccatctt tgtcagatt 
      tattgttgc gcagcaaca caaccaccg gtaattccc 
      ccaccggaa tcgcccaat tcaatttat ggcgataaa 
      tcaacgaat cgtacagtc ctaaatcta gaacaagcg 
      aaattagcc acgccattt cactcgaca accaatgag 
      attgacaaa cgacaaaaa cgagacgct tatgatttg 
      acaggcgag ataagttcg tttgacatg ccagatgac 
      cagattttc ttgattatt gttcaaggt cgtaacttt 
      aacacgacc ttgacgcat aaatatcta atagctcag 
      cctgagccg cataattat tcctattcc gttcctatt 
      tcgaaatat atgcccgac gagttcatt aatctcata 
      ttcagatca actcaagaa tcgatcaag gaggtcgag 
      tattgcaat aataactat aaggaacgt ggatgtgcc 
      tttgagaag ccgcaaaca agacataaa gtcaatgag 
      cacagtccg agtcagttg attagtaca caaagtgtc 
      atttttgag gtcaatatg gccgatttt cagtcgaaa 
      attatgacg taccgagcg tcgagtcga gccaatcag 
      aatacgtgt aaattgagc aacttggat gaatttatc 
      gaatataat ttccatttc taccggcac tgtgatgaa 
      taa
    mRNA sequence
    • >TC015354 RA
      acctccttt aacttgcat tgaaataga aaatcgttc 
      aaaattcat tcaagtgat gcgaaaaaa aaacgtgaa 
      aatgattga gttttgatg tgatatttt gcaagaaat 
      ggacgcatt atcggccga tttggcgct acaggcgat 
      tttaggtcg cggttcgga ctttgtcag cggcatcga 
      taacgaagc ccttgactt ttcccaatt ggaagactt 
      cataaacag cgaaggctc ccaaagtac gaaccctta 
      tttcaacga atcgctaaa ttcgacgcc accttcagg 
      taaactact gaatgttat tgtggaaaa caagcgaaa 
      tgcgggcca ttctttgcc cgaaagccc tcctgatag 
      cagttcaga gcaccctta tagccccca agagggttg 
      cgagccact taacccctc tgagaatat ttacaccac 
      tttggggcc aaatatcta caaaccagt tattggccc 
      catgttggg cgacaattt aattttggg ctcacacat 
      cgtagtagc cgagcaaaa tgggatcat gcaagatac 
      gggattgat gatccctga ggattcgaa tcagaattt 
      tacagatag agggatata taaagggga tgagaattt 
      ggtacattt gggctatac cccggctat tgaaaataa 
      cttgtgtga accgaattt ggataacat acaaacggt 
      ttatacgaa tttacaaag tgcccccaa aaaacgaaa 
      attatcgca agattcgcc tttggtcaa aagtgagcc 
      ggaaaattg cacgacgag tcaattgag ccctcctgg 
      gcctctttc cacaccgtt ggatgaaga gtacgcatc 
      ttctgaagc ttgcctttc tgaatctca atatcaatg 
      tattagatt tagcccttt tcaacagcc acaatggca 
      cgcactctg cgatcaaag tttgaccga attgccggt 
      accacatta tcgagttga tgccgataa aggcttcaa 
      tttttcaaa cgccgacga tgcctttgt atgccagaa 
      aaagaacca tttccagat aacttgcca cgtccaatt 
      attgggtga cgcacaatt tgttaaaac tcccgacgg 
      tttccaaaa gatctcaag cttccatct acactttta 
      cggtgttaa acatgactg tccgactca aacgatacg 
      agttgagca aagtcaaag tgatagaag caaaaaacc 
      atttcatcc cgttttggt cgaacttgt gaattcaca 
      agtaacaaa ggtcacagt aggccgatt acatttcag 
      tgaaacaac gtcgaataa catgcgaaa aaagggtaa 
      accgaatcc tgaacagcg atattttca gttggttgt 
      aggtctaca tgcacatac tacaaatgg taattttcc 
      ggttataag tcacgcgag tcaaaaaat aattgtgag 
      ggcttcaaa tcctggcca atttgaaag tgacgttga 
      attgtgttg gcaaaaagg ccagacgca agactcaat 
      ttttcataa tgggaaagt tggtattaa tacggacag 
      accggacga atcattggt cgtccatgg taatattaa 
      agtaactgg acatattat acaaccaag tgacataag 
      ggcgaagaa aaatatcgt cgaatgtga cacggctga 
      gcagctacg aaacgtcca aaaactcag agttgttag 
      gtatgacta cgaaccgtc gtttgcatc tcagttaag 
      tcgtgattc acaacactc ggataccgg tgtgatagc 
      tcaggaagt ggctaaaat cctcccgga agcggtcag 
      accggctgg tgaccttgt cttaaagaa tggtcaatc 
      cattgataa ttttttggt agtcaacaa ggagcgcat 
      ttttatgga aaatatcgg tgctgttaa ggaattatg 
      taaagtgac tgataattt ggagactcg aatcgatca 
      attagaacg aatcaatcg ccgattgaa taaattgaa 
      acgtggtga tagtctcaa atcgactag tacagtgag 
      tagcttgaa acattccaa ttcaatgca aaaatgttc 
      caaacatca ttgtgaccg tgacgacga actctgttc 
      gaataaatt tattcaaat aattatcgt cattctggt 
      tcttataat ggcattttg tttggccgc tataacaac 
      cttgtacct gatggaagc ctcacgaca caaccaatt 
      cgtccgaac taacctcca acaaaccca tcggtcgtc 
      catagtaac caaacgaca aatgaaagt gccgaaaaa 
      ccatcaatc acgacacac cgaatacca caaaacaag 
      taacaataa ttacaaaga cgatgacgt cacacgtat 
      caccataga tccgagttt aacgaaacg gccttcggc 
      tatcatcgg ccgaccacc cgattgtct atcggttga 
      tcaagacct tgaaagtag ccatctttg tcagattta 
      ttgttgcgc agcaacaca accaccggt aattccccc 
      accggaatc gcccaattc aatttatgg cgataaatc 
      aacgaatcg tacagtcct aaatctaga acaagcgaa 
      attagccac gccatttca ctcgacaac caatgagat 
      tgacaaacg acaaaaacg agacgctta tgatttgac 
      aggcgagat aagttcgtt tgacatgcc agatgacca 
      gattttctt gattattgt tcaaggtcg taactttaa 
      cacgacctt gacgcataa atatctaat agctcagcc 
      tgagccgca taattattc ctattccgt tcctatttc 
      gaaatatat gcccgacga gttcattaa tctcatatt 
      cagatcaac tcaagaatc gatcaagga ggtcgagta 
      ttgcaataa taactataa ggaacgtgg atgtgcctt 
      tgagaagcc gcaaacaag acataaagt caatgagca 
      cagtccgag tcagttgat tagtacaca aagtgtcat 
      ttttgaggt caatatggc cgattttca gtcgaaaat 
      tatgacgta ccgagcgtc gagtcgagc caatcagaa 
      tacgtgtaa attgagcaa cttggatga atttatcga 
      atataattt ccatttcta ccggcactg tgatgaata 
      aacgatacg tactactca aaaaaagga aactaagaa 
      caagaaatg catgacatt tatttattt acgtgtttt 
      tttttaaat attatattt ttttaattg ttttatcaa 
      agtgttcga tgttactac agcatcacc tagttgtag 
      aagtacctg tatctgaaa gatttgaac acttttcta 
      gttagtgta aaatgaaaa agcaccaaa aaaatattg 
      tagcgtttt tagattatg actcatata atgtactta 
      attttctcg tccgtccaa taaattgta taatgtgta 
      gattagact tttatcgta ttgtaaaat atttttctt 
      gtttgacaa atttttatt attttatag aattaaaaa 
      tataaatga aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY