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Gene information
General information
TC015351 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 102777..110074
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015351
    iBeetle screen iB_05800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015351 RA
      MILSPDMSL KTYKKRSGT SDQGKEYEW LLCVYYAIQ 
      LSLSSEISD FELCTNNAF FGDFDDVTL RVRFKDGVV 
      KVYLLQTKH SEQPKTVTV KALTSDEGG LTIYKYEKS 
      YENTLQLSN FVYILFTNR TAKFKDKTQ LDEKISLRH 
      SQCVARNFL NTAKNSVYK FESEQNSDF YQRFYLYTN 
      QKNVSNLHK SIADLLQTH FQTDIKSTF VDFMRMWWA 
      RNFMLSKSD IVAKLVELT LSPMIQTLT CDIDNEKTK 
      LLQAAIMRF DLTLVEQNG GSLITQIWH PTLDNHTIK 
      TVSMTGLKY GLVTQGVSN LKQLTRDQQ SRIAWYMNL 
      WPLVVKVDE TKRNQLNCA LNLLKQNKK RVVLLDHDV 
      DMNGFHIFR NLSDLLEGD TLRSAIMQK FNCSLQGKL 
      DVTLAELGS CLMSHFKVD QLVQIATGT NFNIGEPLE 
      ILPPTYIER KFPKIILDC KVIEDSKQW KICLSCNNQ 
      KEQFREKIT KRMVKITTA VTGFANFPK HYVYATDGE 
      YPKELTEEK YRYYKADCH FRVTGQNVQ WLSNRDRCY 
      EFINEIVKY VKNSNEICT ERQLDDNFH CNVKVLTSH 
      TGSGKTSLL KSVKNRALT NCFVMLFDL REHSTFFQK 
      SQTSKEVWN YVTKFYAKN RKYNEFETG LLKEFPKQQ 
      TLILWDSFD ELSFGSREK FVKVVSELR MLGVSQWIT 
      SKNHFRQSL ENEFQVFAL SMVPFHQID QIQYLEKRC 
      GVSVNLIES NFLSNCQIL DNQEYLGIP LQLYIISDI 
      FNKNPKLLE GVYSLADMF RALIEGRYQ HFLSKHPNH 
      EFLTHIIND SREYRLDQY KIAAVKTHL SHVYDKLNL 
      KNTARFIED VQNGDFLGL VNCQDDEFV FEFKILGEY 
      LAALFLSEN CQMLDLEFI FEVKYRNIR FLFDLIMAE 
      GQPDLISLL YKNFDRFDN FDTRDKMGR NALHLICSY 
      GIKHPLLRT EDEKYAFSQ GDLVHEDAP LVKQLVKNL 
      TLKCDVGEK DKLFNWTCF DYADRSLSL GLLELLPNL 
      DNVLPTLSN FNDMSTVLY YALKFDYQN LFKSAQRDI 
      TFVKDTNGV NFLHVATEF GREHFLTWL LTQKVYIQG 
      INMQNSSHW APIQIASFN GNLTFLKLL QTKGATFPL 
      RDPSLVSLA TSHPHRHIL DFLIECECS VNEFYHHNY 
      FTRSLPLAI SHGLVDIVK FLVKNGAKF YNVGKNNMN 
      ALHMALEHQ QYEIADVFL EHGINIDQV DGKQQSSVH 
      HAAQNNHPD VIEWLLARG AKINIFDKN QRNALHWAA 
      HSGSLEAAK VLIKAGIDI DVSDDQGLT PLHLAVLSG 
      QLSIVTLLL DAKCDFKPV NVYKRTPLH LAAMNDLTD 
      IVQVLLQQS NSVDCVESL YGRTPLHYA AWNGHPDCV 
      KTLLLSNAS FDIRCNFGY TPLHLATEA ENYDCCRLL 
      LEAGARFDI ANRNGTTAL DLVNSLDIF MLMMTFDTY 
      PIHTATSKG NYVAIPEIV TLGHDINQF DSQGMRPLH 
      LAVNLYQPF NTVRTLLDN GANVNLRDK DGYKPLHHL 
      LQGRCPDRD TFDLLVTGR GELEINAKT VKNLTPLHI 
      AAQAGCFIL VPGEDTIFE DLTQLLLDK GANIEACDD 
      DGFTPLHYA AQSGNLTIV KLLTRDNLI NQINRYIRT 
      PLHMAAVKG HHFVVKYLI DRGAQQIND YEGDTPLDD 
      ALKIESIET IKLLTNDLS VTNRKKRNV LHMAAKQGL 
      LDLVETFQK DGIMTSEDC DGTSPAEEA LYNRHFKVF 
      DIMDLSNFD VNKYSRRYK KYPLHVATN FQKLRLVEK 
      FVEMGARTD VCDSDGNTP FYNCDDFYI LQLLVKSDN 
      SGLNVQNNK GETVLHFLA KLKHILLEF LVDQGGDVN 
      LMDNDGNTV LDILLNRGI IVKNVLFKT NGFDWNKTN 
      RNGDTLLHI AINYNMADV VQWLLDRGA STGIKNLVI 
      YALRNSHAE IVRLLIKYC DLSQEEDLI YYTVRYGWK 
      LLPELRPHI SPSALTPSE QTDLLESVC HVDYFMDVW 
      DLLPDADVS TMATDLGDT PLHLAVSRG NLTLTKRLL 
      KSGLKPNDA NRSGETPTQ CAVKRGHFS ILYLLLEND 
      KNAVIRSDQ KGNTLLHDA ASEGNLEIV KFLLKKGFR 
      NDICNGRMC TAADVAKEK GHEEVYQFL KNL
    CDS sequence
    • >TC015351 RA
      atgattctt tcgccagat atgtcattg aagacctat 
      aaaaaaaga agcggcact tccgaccaa ggcaaggaa 
      tacgaatgg ctgctctgc gtttattac gctatacaa 
      ttatccttg agttcggaa atttccgac tttgagctg 
      tgtacaaat aatgcattt ttcggtgat ttcgatgat 
      gtcacctta agagttcgc ttcaaagat ggcgtcgtt 
      aaggtttac cttctccaa acaaaacac agcgaacaa 
      ccaaaaacc gtcactgtt aaagctctg acgtcagac 
      gaaggcggt ttgacaatc tacaaatac gaaaaatcc 
      tacgaaaat accctacaa ctgtcaaac tttgtctac 
      attttgttt acaaatcgg acagccaaa ttcaaggac 
      aaaacacag cttgatgag aagattagt ttaagacat 
      tcgcaatgt gttgctcga aactttctc aacactgcc 
      aaaaattca gtctataaa tttgaaagc gagcaaaat 
      tccgacttt taccaaagg ttttatctc tataccaat 
      caaaaaaac gtttcgaat ttacacaaa tcaatcgcc 
      gatttactc caaactcac tttcaaacc gacatcaaa 
      tcaactttc gtcgatttc atgcgaatg tggtgggcg 
      agaaatttc atgttgtca aaatcagac attgttgcc 
      aaattggtt gaattgaca ctatcacca atgatacaa 
      acgctaacg tgtgacatt gacaacgaa aaaaccaaa 
      ctactacaa gccgccatt atgcgattt gatttgaca 
      ttagtcgaa caaaatggc ggttcgttg ataacacaa 
      atatggcat ccgacactt gacaaccat acaatcaaa 
      actgtcagt atgacaggt ctcaagtac ggtctagtt 
      acccaaggt gtgtcaaat ttgaagcaa ttgacacgt 
      gaccaacaa tcacgcatc gcttggtac atgaatttg 
      tggcctttg gtcgtaaaa gtcgacgaa accaaacga 
      aatcaactc aactgtgct ttaaacctt ttaaaacaa 
      aacaaaaaa cgtgttgtt ttattggac catgatgtt 
      gatatgaat ggttttcac attttccgg aacttgtca 
      gatttgctt gaaggtgac actttacgt tctgcgatt 
      atgcaaaaa ttcaactgt tcgttacaa ggcaaactt 
      gatgtgaca ctagccgaa cttgggtcg tgtctaatg 
      tcacacttc aaggttgac cagcttgtc caaattgcc 
      actggcacc aactttaac attggagag ccactagaa 
      atattgccg ccaacttac atcgagcga aaattcccc 
      aaaattatc ctcgactgt aaagtgatt gaggattcc 
      aaacagtgg aaaatctgt ctcagttgt aacaaccaa 
      aaggagcaa tttcgcgaa aaaattact aaacgtatg 
      gttaagatc acaacggct gtgactggt tttgctaat 
      tttcccaag cattatgtc tatgctact gatggggaa 
      taccctaag gaactaaca gaggaaaaa tatcgttat 
      tacaaggcc gattgtcac ttccgtgtc actggacag 
      aacgtacag tggttgtca aatcgtgat aggtgttat 
      gaatttatc aacgaaatt gtcaaatat gtcaagaat 
      tcgaatgaa atttgcacc gagcgacaa cttgacgat 
      aatttccat tgtaatgtc aaagttttg acatcgcat 
      acgggcagt ggcaaaacc agtcttttg aaaagtgtc 
      aaaaaccga gctttgact aattgcttt gtcatgttg 
      tttgacttg agggaacat tcaacattt ttccaaaag 
      tctcagact tcgaaggaa gtttggaat tatgtgaca 
      aaattttac gccaaaaat cgtaaatat aacgaattt 
      gagactgga cttctcaag gaatttcca aaacaacaa 
      actttaata ctttgggat agtttcgac gaattgtca 
      ttcgggtca agggaaaaa tttgtcaaa gttgtgtca 
      gaacttcgc atgttggga gtttctcaa tggataact 
      tcgaaaaat cattttcgt caatctctg gaaaacgaa 
      tttcaagtt ttcgcatta tctatggta ccgttccac 
      caaatcgac caaatccaa tacctggag aagcgctgt 
      ggtgtcagt gttaattta atcgagtca aatttcttg 
      tcaaattgt caaatctta gacaatcag gagtacctg 
      ggcattccc ctccaactc tacatcatt agcgacatc 
      ttcaataaa aaccctaaa ctactcgaa ggggtttac 
      agtcttgcc gacatgttt cgtgcatta attgaggga 
      cgttaccaa catttttta tcaaaacac cccaatcat 
      gaatttttg acacacatt attaatgat agtcgagag 
      taccgtttg gaccaatac aaaatagct gctgtcaag 
      acacaccta agtcatgtt tatgacaaa ctcaacttg 
      aaaaatacc gcacgtttt atagaagac gtacaaaat 
      ggcgatttt ttgggactt gtcaattgt caagatgac 
      gagtttgtt tttgagttt aaaatactt ggggaatat 
      cttgctgca ttgtttctt tcggaaaat tgtcaaatg 
      ttggatctt gaatttatt tttgaagtc aaatatcga 
      aatattcgc tttttattc gatttgatt atggctgag 
      ggtcagcca gatttaatt tcactactt tataaaaac 
      tttgacagg tttgacaat tttgacacg agggacaaa 
      atgggtcga aatgcccta cacttgatt tgttcttac 
      ggcataaag cacccactt ttacgcacg gaagacgag 
      aaatatgcc ttcagtcaa ggggatctg gtacatgaa 
      gatgcccca cttgtcaaa caacttgtc aaaaatttg 
      acactcaag tgtgacgtt ggggaaaaa gacaaattg 
      ttcaactgg acgtgtttt gattacgcc gatcgttca 
      ctctcactt ggccttctc gaacttttg ccaaatctt 
      gacaacgtt cttccaaca ttgtcaaat ttcaatgac 
      atgtccacc gtactgtat tatgccctt aaatttgac 
      taccaaaac ttattcaaa tctgcacaa agagatata 
      acgtttgtg aaagacacc aatggtgtt aatttcctt 
      catgttgca acagaattc ggtcgtgag cattttttg 
      acatggctt ttgacacaa aaagtctac atccaaggc 
      attaatatg caaaattcg agtcattgg gcaccgatt 
      caaattgcc tcctttaat ggcaatttg acatttttg 
      aagcttttg caaaccaaa ggtgcaacg tttccatta 
      cgtgatccc tcacttgtg tcactcgct acgtcgcac 
      cctcatcgt cacattctc gactttttg atagaatgt 
      gaatgtagc gtgaatgaa ttctaccat cacaattac 
      ttcacacga tcgttacct ctggcgata agtcatggg 
      ctagttgac attgttaaa tttttagtc aaaaacggg 
      gcaaaattc tacaacgtt ggcaaaaac aacatgaat 
      gccttgcat atggcttta gaacatcaa caatatgaa 
      attgcggac gtgttcctt gaacatggc attaatatt 
      gatcaagtt gacggcaag caacaatca agtgttcat 
      catgccgca caaaacaac caccctgat gtaatcgag 
      tggttgttg gcacgtggg gcaaaaatt aatattttt 
      gacaagaat caacgcaat gctttgcat tgggcggca 
      cattccggg tcacttgaa gccgcaaaa gtgctgatc 
      aaagctgga attgacatt gatgtttct gatgatcaa 
      ggtttgaca ccgttacat ttagcagta ttgtcagga 
      caattgtca attgttacg ttactactg gacgcaaag 
      tgtgacttt aaaccagtc aatgtgtac aaaaggaca 
      ccgttacat cttgccgca atgaacgat ttgactgac 
      attgtccaa gttttacta caacagtca aattcagtt 
      gactgtgtt gaaagtctc tacggacga acaccgtta 
      cattatgcc gcttggaac ggtcatcct gactgtgtc 
      aaaacccta ctattgtca aatgccagt tttgacatt 
      cgatgcaat ttcggttac acaccgtta catcttgcc 
      acagaagcg gaaaattac gattgttgt cgtttgtta 
      ctcgaagct ggtgctaga tttgacatt gctaaccgt 
      aacggtact acagcactc gatctagtt aacagtctt 
      gacattttc atgctcatg atgacattt gacacatat 
      ccaatacat acagcaacg tctaaaggc aattacgtt 
      gcaatccct gagattgta acacttgga catgacatc 
      aaccaattt gacagtcaa gggatgaga ccgttacac 
      ctagctgtc aatttgtac caaccgttc aacactgtc 
      agaactcta cttgacaat ggagccaat gtcaatttg 
      cgagacaaa gatgggtat aaacctctc catcacttg 
      ttacaaggt cgttgtccc gatcgagac acttttgac 
      ttacttgta acgggtcga ggtgagctt gaaatcaac 
      gcaaaaact gtcaaaaac ttgacaccg ttacatatc 
      gcagcacag gccggttgt tttatacta gtaccaggt 
      gaagacaca atttttgaa gatttgaca cagctattg 
      cttgacaaa ggggcaaat attgaagca tgtgatgat 
      gatggtttt acaccgtta cattacgca gcacagagt 
      ggtaatttg acaattgtc aaacttttg acacgtgac 
      aatttaatt aatcaaatc aatcgatac attcggaca 
      ccgttacat atggctgct gtcaaaggt caccacttt 
      gttgtcaaa tatttaata gatagaggg gcacaacaa 
      atcaatgac tacgaaggt gatactcca ctagatgat 
      gctttaaaa attgaaagt attgaaact atcaaatta 
      ttaacgaat gatttgtct gtgaccaat cgtaaaaaa 
      cggaacgtt ttacatatg gctgcgaaa caaggcctg 
      cttgattta gtcgaaaca tttcaaaaa gatggaatt 
      atgactagt gaggattgt gatggaaca tcaccagct 
      gaggaagct ttatataac cgccatttt aaggttttt 
      gacattatg gacttgtca aattttgac gttaataaa 
      tacagtcga cggtataag aaatatcca cttcatgtg 
      gccactaat tttcaaaaa ctaagacta gttgagaag 
      tttgtcgaa atgggtgcc agaactgac gtgtgtgac 
      agtgatggc aatacgccg ttttataat tgtgatgat 
      ttctacatt ttgcaattg ttggtcaag tctgacaat 
      agtggtcta aatgtgcaa aataacaag ggagagacc 
      gttttacat tttttggca aaattgaaa cacatttta 
      ttggaattt ttggtggat caaggtggc gatgtcaat 
      ttaatggac aatgacggt aacactgtt ttggatata 
      ttgttgaat cgcgggatt atcgtgaaa aatgtgttg 
      tttaaaacg aatggtttt gactggaac aaaacaaat 
      cgtaatggt gatacttta ctccatatt gctatcaat 
      tacaatatg gcggatgtc gtgcaatgg ctacttgat 
      cgaggtgcc tcaactgga ataaaaaat cttgtgatt 
      tacgcttta cgaaattct catgctgaa attgttcga 
      ttattaata aaatattgt gatttgagt caggaggag 
      gatcttatt tactatact gtacgatac ggatggaaa 
      ctcctccca gaactccgc ccacatatc tccccctcc 
      gctctcaca ccaagcgag caaaccgat ctcctcgaa 
      tcagtttgt cacgttgat tatttcatg gacgtgtgg 
      gatttactc cccgacgct gatgtctcc accatggca 
      accgatctc ggtgacaca cctctacat ttggctgtt 
      tctcgtggc aacttgact ttgactaaa cgtttactt 
      aaaagcggt ttaaaaccg aatgatgct aatcgtagt 
      ggtgaaaca ccgacacaa tgtgcagtc aagagagga 
      catttctca attttatac cttttactc gagaatgat 
      aaaaatgca gtgattcga agcgaccag aaaggaaat 
      actttacta catgatgca gcaagtgag gggaatttg 
      gaaattgtc aagtttcta ctaaagaag ggctttcgg 
      aacgatatt tgcaatggg agaatgtgt actgcagcc 
      gatgtagca aaggagaag ggacatgaa gaggtttac 
      cagtttttg aaaaatttg taa
    mRNA sequence
    • >TC015351 RA
      tagacagtt ggttttttc actgagcac attttcttg 
      ttgaaatca cgcaaaaaa atgaatgaa tgtttgtgt 
      agtgcattt ttgtgatga ttctttcgc cagatatgt 
      cattgaaga cctataaaa aaagaagcg gcacttccg 
      accaaggca aggaatacg aatggctgc tctgcgttt 
      attacgcta tacaattat ccttgagtt cggaaattt 
      ccgactttg agctgtgta caaataatg catttttcg 
      gtgatttcg atgatgtca ccttaagag ttcgcttca 
      aagatggcg tcgttaagg tttaccttc tccaaacaa 
      aacacagcg aacaaccaa aaaccgtca ctgttaaag 
      ctctgacgt cagacgaag gcggtttga caatctaca 
      aatacgaaa aatcctacg aaaataccc tacaactgt 
      caaactttg tctacattt tgtttacaa atcggacag 
      ccaaattca aggacaaaa cacagcttg atgagaaga 
      ttagtttaa gacattcgc aatgtgttg ctcgaaact 
      ttctcaaca ctgccaaaa attcagtct ataaatttg 
      aaagcgagc aaaattccg acttttacc aaaggtttt 
      atctctata ccaatcaaa aaaacgttt cgaatttac 
      acaaatcaa tcgccgatt tactccaaa ctcactttc 
      aaaccgaca tcaaatcaa ctttcgtcg atttcatgc 
      gaatgtggt gggcgagaa atttcatgt tgtcaaaat 
      cagacattg ttgccaaat tggttgaat tgacactat 
      caccaatga tacaaacgc taacgtgtg acattgaca 
      acgaaaaaa ccaaactac tacaagccg ccattatgc 
      gatttgatt tgacattag tcgaacaaa atggcggtt 
      cgttgataa cacaaatat ggcatccga cacttgaca 
      accatacaa tcaaaactg tcagtatga caggtctca 
      agtacggtc tagttaccc aaggtgtgt caaatttga 
      agcaattga cacgtgacc aacaatcac gcatcgctt 
      ggtacatga atttgtggc ctttggtcg taaaagtcg 
      acgaaacca aacgaaatc aactcaact gtgctttaa 
      accttttaa aacaaaaca aaaaacgtg ttgttttat 
      tggaccatg atgttgata tgaatggtt ttcacattt 
      tccggaact tgtcagatt tgcttgaag gtgacactt 
      tacgttctg cgattatgc aaaaattca actgttcgt 
      tacaaggca aacttgatg tgacactag ccgaacttg 
      ggtcgtgtc taatgtcac acttcaagg ttgaccagc 
      ttgtccaaa ttgccactg gcaccaact ttaacattg 
      gagagccac tagaaatat tgccgccaa cttacatcg 
      agcgaaaat tccccaaaa ttatcctcg actgtaaag 
      tgattgagg attccaaac agtggaaaa tctgtctca 
      gttgtaaca accaaaagg agcaatttc gcgaaaaaa 
      ttactaaac gtatggtta agatcacaa cggctgtga 
      ctggttttg ctaattttc ccaagcatt atgtctatg 
      ctactgatg gggaatacc ctaaggaac taacagagg 
      aaaaatatc gttattaca aggccgatt gtcacttcc 
      gtgtcactg gacagaacg tacagtggt tgtcaaatc 
      gtgataggt gttatgaat ttatcaacg aaattgtca 
      aatatgtca agaattcga atgaaattt gcaccgagc 
      gacaacttg acgataatt tccattgta atgtcaaag 
      ttttgacat cgcatacgg gcagtggca aaaccagtc 
      ttttgaaaa gtgtcaaaa accgagctt tgactaatt 
      gctttgtca tgttgtttg acttgaggg aacattcaa 
      catttttcc aaaagtctc agacttcga aggaagttt 
      ggaattatg tgacaaaat tttacgcca aaaatcgta 
      aatataacg aatttgaga ctggacttc tcaaggaat 
      ttccaaaac aacaaactt taatacttt gggatagtt 
      tcgacgaat tgtcattcg ggtcaaggg aaaaatttg 
      tcaaagttg tgtcagaac ttcgcatgt tgggagttt 
      ctcaatgga taacttcga aaaatcatt ttcgtcaat 
      ctctggaaa acgaatttc aagttttcg cattatcta 
      tggtaccgt tccaccaaa tcgaccaaa tccaatacc 
      tggagaagc gctgtggtg tcagtgtta atttaatcg 
      agtcaaatt tcttgtcaa attgtcaaa tcttagaca 
      atcaggagt acctgggca ttcccctcc aactctaca 
      tcattagcg acatcttca ataaaaacc ctaaactac 
      tcgaagggg tttacagtc ttgccgaca tgtttcgtg 
      cattaattg agggacgtt accaacatt ttttatcaa 
      aacacccca atcatgaat ttttgacac acattatta 
      atgatagtc gagagtacc gtttggacc aatacaaaa 
      tagctgctg tcaagacac acctaagtc atgtttatg 
      acaaactca acttgaaaa ataccgcac gttttatag 
      aagacgtac aaaatggcg attttttgg gacttgtca 
      attgtcaag atgacgagt ttgtttttg agtttaaaa 
      tacttgggg aatatcttg ctgcattgt ttctttcgg 
      aaaattgtc aaatgttgg atcttgaat ttatttttg 
      aagtcaaat atcgaaata ttcgctttt tattcgatt 
      tgattatgg ctgagggtc agccagatt taatttcac 
      tactttata aaaactttg acaggtttg acaattttg 
      acacgaggg acaaaatgg gtcgaaatg ccctacact 
      tgatttgtt cttacggca taaagcacc cacttttac 
      gcacggaag acgagaaat atgccttca gtcaagggg 
      atctggtac atgaagatg ccccacttg tcaaacaac 
      ttgtcaaaa atttgacac tcaagtgtg acgttgggg 
      aaaaagaca aattgttca actggacgt gttttgatt 
      acgccgatc gttcactct cacttggcc ttctcgaac 
      ttttgccaa atcttgaca acgttcttc caacattgt 
      caaatttca atgacatgt ccaccgtac tgtattatg 
      cccttaaat ttgactacc aaaacttat tcaaatctg 
      cacaaagag atataacgt ttgtgaaag acaccaatg 
      gtgttaatt tccttcatg ttgcaacag aattcggtc 
      gtgagcatt ttttgacat ggcttttga cacaaaaag 
      tctacatcc aaggcatta atatgcaaa attcgagtc 
      attgggcac cgattcaaa ttgcctcct ttaatggca 
      atttgacat ttttgaagc ttttgcaaa ccaaaggtg 
      caacgtttc cattacgtg atccctcac ttgtgtcac 
      tcgctacgt cgcaccctc atcgtcaca ttctcgact 
      ttttgatag aatgtgaat gtagcgtga atgaattct 
      accatcaca attacttca cacgatcgt tacctctgg 
      cgataagtc atgggctag ttgacattg ttaaatttt 
      tagtcaaaa acggggcaa aattctaca acgttggca 
      aaaacaaca tgaatgcct tgcatatgg ctttagaac 
      atcaacaat atgaaattg cggacgtgt tccttgaac 
      atggcatta atattgatc aagttgacg gcaagcaac 
      aatcaagtg ttcatcatg ccgcacaaa acaaccacc 
      ctgatgtaa tcgagtggt tgttggcac gtggggcaa 
      aaattaata tttttgaca agaatcaac gcaatgctt 
      tgcattggg cggcacatt ccgggtcac ttgaagccg 
      caaaagtgc tgatcaaag ctggaattg acattgatg 
      tttctgatg atcaaggtt tgacaccgt tacatttag 
      cagtattgt caggacaat tgtcaattg ttacgttac 
      tactggacg caaagtgtg actttaaac cagtcaatg 
      tgtacaaaa ggacaccgt tacatcttg ccgcaatga 
      acgatttga ctgacattg tccaagttt tactacaac 
      agtcaaatt cagttgact gtgttgaaa gtctctacg 
      gacgaacac cgttacatt atgccgctt ggaacggtc 
      atcctgact gtgtcaaaa ccctactat tgtcaaatg 
      ccagttttg acattcgat gcaatttcg gttacacac 
      cgttacatc ttgccacag aagcggaaa attacgatt 
      gttgtcgtt tgttactcg aagctggtg ctagatttg 
      acattgcta accgtaacg gtactacag cactcgatc 
      tagttaaca gtcttgaca ttttcatgc tcatgatga 
      catttgaca catatccaa tacatacag caacgtcta 
      aaggcaatt acgttgcaa tccctgaga ttgtaacac 
      ttggacatg acatcaacc aatttgaca gtcaaggga 
      tgagaccgt tacacctag ctgtcaatt tgtaccaac 
      cgttcaaca ctgtcagaa ctctacttg acaatggag 
      ccaatgtca atttgcgag acaaagatg ggtataaac 
      ctctccatc acttgttac aaggtcgtt gtcccgatc 
      gagacactt ttgacttac ttgtaacgg gtcgaggtg 
      agcttgaaa tcaacgcaa aaactgtca aaaacttga 
      caccgttac atatcgcag cacaggccg gttgtttta 
      tactagtac caggtgaag acacaattt ttgaagatt 
      tgacacagc tattgcttg acaaagggg caaatattg 
      aagcatgtg atgatgatg gttttacac cgttacatt 
      acgcagcac agagtggta atttgacaa ttgtcaaac 
      ttttgacac gtgacaatt taattaatc aaatcaatc 
      gatacattc ggacaccgt tacatatgg ctgctgtca 
      aaggtcacc actttgttg tcaaatatt taatagata 
      gaggggcac aacaaatca atgactacg aaggtgata 
      ctccactag atgatgctt taaaaattg aaagtattg 
      aaactatca aattattaa cgaatgatt tgtctgtga 
      ccaatcgta aaaaacgga acgttttac atatggctg 
      cgaaacaag gcctgcttg atttagtcg aaacatttc 
      aaaaagatg gaattatga ctagtgagg attgtgatg 
      gaacatcac cagctgagg aagctttat ataaccgcc 
      attttaagg tttttgaca ttatggact tgtcaaatt 
      ttgacgtta ataaataca gtcgacggt ataagaaat 
      atccacttc atgtggcca ctaattttc aaaaactaa 
      gactagttg agaagtttg tcgaaatgg gtgccagaa 
      ctgacgtgt gtgacagtg atggcaata cgccgtttt 
      ataattgtg atgatttct acattttgc aattgttgg 
      tcaagtctg acaatagtg gtctaaatg tgcaaaata 
      acaagggag agaccgttt tacattttt tggcaaaat 
      tgaaacaca ttttattgg aatttttgg tggatcaag 
      gtggcgatg tcaatttaa tggacaatg acggtaaca 
      ctgttttgg atatattgt tgaatcgcg ggattatcg 
      tgaaaaatg tgttgttta aaacgaatg gttttgact 
      ggaacaaaa caaatcgta atggtgata ctttactcc 
      atattgcta tcaattaca atatggcgg atgtcgtgc 
      aatggctac ttgatcgag gtgcctcaa ctggaataa 
      aaaatcttg tgatttacg ctttacgaa attctcatg 
      ctgaaattg ttcgattat taataaaat attgtgatt 
      tgagtcagg aggaggatc ttatttact atactgtac 
      gatacggat ggaaactcc tcccagaac tccgcccac 
      atatctccc cctccgctc tcacaccaa gcgagcaaa 
      ccgatctcc tcgaatcag tttgtcacg ttgattatt 
      tcatggacg tgtgggatt tactccccg acgctgatg 
      tctccacca tggcaaccg atctcggtg acacacctc 
      tacatttgg ctgtttctc gtggcaact tgactttga 
      ctaaacgtt tacttaaaa gcggtttaa aaccgaatg 
      atgctaatc gtagtggtg aaacaccga cacaatgtg 
      cagtcaaga gaggacatt tctcaattt tataccttt 
      tactcgaga atgataaaa atgcagtga ttcgaagcg 
      accagaaag gaaatactt tactacatg atgcagcaa 
      gtgagggga atttggaaa ttgtcaagt ttctactaa 
      agaagggct ttcggaacg atatttgca atgggagaa 
      tgtgtactg cagccgatg tagcaaagg agaagggac 
      atgaagagg tttaccagt ttttgaaaa atttgtaat 
      aaagtttct gggggaaaa accgtgttt tatttgttt 
      ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY