Status
Please wait ...
Gene information
General information
TC015334 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 284267..285572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015334
    iBeetle screen iB_05798

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015334 RA
      MGDSVAELL QFVHLGARL DLKSVALGH ILGLTGNAD 
      GIATLINTP SLLVSLITL LSDNNATIV KDSALCLVN 
      VSADHKGAL ALLDLDTSV GCPPLQSPP SDITSTVVK 
      FVTDPMSKI ADFSCMILS NLSRANHDK VIDALEKSA 
      SLDDLIDIF TKKQYNKAG ANLHYLSPL FSNLSQSGR 
      VRRRFLDRI HKLVPFIEF EESAVRRGG VVGALRNCT 
      FDPECHELL LGPHVDILP RLLRPLAGN EEFDDEDND 
      KLPLELQYL PEEKQREED PDIRCMLLE ALTQLCAKR 
      KNREFIRER NTYVILREL HKWEKDRKV LLACENLVD 
      ILIRTEEEI GEDDLKTLK VPQSLSEDF NKADEQFLK 
      DE
    CDS sequence
    • >TC015334 RA
      atgggtgat tccgtggcc gaattgttg cagtttgtg 
      catctgggg gcccgtttg gacctcaaa tcggtggct 
      ttggggcac attttgggt ttgacgggg aatgcggac 
      ggaattgcc acattaatc aacacaccg tcacttctg 
      gtctcctta atcacactt ttgagtgat aataacgcc 
      acaattgtc aaagactcg gcactttgc cttgttaat 
      gtctctgct gatcacaag ggggccctg gcattgctt 
      gatttggac acgagtgtg gggtgcccc cctttacag 
      agccccccg agtgacatt acttcgact gttgttaaa 
      ttcgtgacg gaccccatg agtaaaatc gccgatttt 
      tcctgcatg attttatcg aacttatct cgggccaat 
      cacgataaa gtgattgat gcgcttgag aaaagcgca 
      agtttggac gatttgatt gacattttc acgaaaaaa 
      cagtacaat aaagcgggg gctaacctc cactacttg 
      agccccctt ttttcgaac ttgtcgcaa agtgggcgc 
      gtccggaga cgcttcctg gaccggatc cacaaactc 
      gtgcctttc attgaattc gaggagtcg gccgtgagg 
      cgcgggggc gtcgtgggg gccctgcgt aactgcaca 
      tttgacccc gagtgtcat gagctgctc ttgggcccc 
      catgtcgat atcttgccc cggctgctc cgccccctg 
      gccggaaac gaggaattt gacgacgaa gacaacgac 
      aagctcccc cttgaactg caatatctc ccggaggag 
      aaacagcgt gaagaagat ccagatatc aggtgcatg 
      ttactggag gctttgacc cagttatgt gccaagaga 
      aaaaatcga gaatttatc agagagaga aacacttat 
      gtgattttg cgcgagttg cacaagtgg gaaaaggac 
      agaaaggtg cttttagcg tgtgaaaat ttggtcgat 
      attttgata cgcaccgag gaggaaatt ggagaagat 
      gatttgaag acacttaaa gtgccccaa agtttaagc 
      gaagacttc aataaagcg gatgagcag tttttaaaa 
      gatgaataa
    mRNA sequence
    • >TC015334 RA
      taaaaaaat aatgcctta cttattaga atgattttt 
      tgccaccta accccactt ttttgacag ctgtcaccc 
      ctcgtgttt tgttttttt ttgggggct ttagcctga 
      taatgggtg attccgtgg ccgaattgt tgcagtttg 
      tgcatctgg gggcccgtt tggacctca aatcggtgg 
      ctttggggc acattttgg gtttgacgg ggaatgcgg 
      acggaattg ccacattaa tcaacacac cgtcacttc 
      tggtctcct taatcacac ttttgagtg ataataacg 
      ccacaattg tcaaagact cggcacttt gccttgtta 
      atgtctctg ctgatcaca agggggccc tggcattgc 
      ttgatttgg acacgagtg tggggtgcc cccctttac 
      agagccccc cgagtgaca ttacttcga ctgttgtta 
      aattcgtga cggacccca tgagtaaaa tcgccgatt 
      tttcctgca tgattttat cgaacttat ctcgggcca 
      atcacgata aagtgattg atgcgcttg agaaaagcg 
      caagtttgg acgatttga ttgacattt tcacgaaaa 
      aacagtaca ataaagcgg gggctaacc tccactact 
      tgagccccc ttttttcga acttgtcgc aaagtgggc 
      gcgtccgga gacgcttcc tggaccgga tccacaaac 
      tcgtgcctt tcattgaat tcgaggagt cggccgtga 
      ggcgcgggg gcgtcgtgg gggccctgc gtaactgca 
      catttgacc ccgagtgtc atgagctgc tcttgggcc 
      cccatgtcg atatcttgc cccggctgc tccgccccc 
      tggccggaa acgaggaat ttgacgacg aagacaacg 
      acaagctcc cccttgaac tgcaatatc tcccggagg 
      agaaacagc gtgaagaag atccagata tcaggtgca 
      tgttactgg aggctttga cccagttat gtgccaaga 
      gaaaaaatc gagaattta tcagagaga gaaacactt 
      atgtgattt tgcgcgagt tgcacaagt gggaaaagg 
      acagaaagg tgcttttag cgtgtgaaa atttggtcg 
      atattttga tacgcaccg aggaggaaa ttggagaag 
      atgatttga agacactta aagtgcccc aaagtttaa 
      gcgaagact tcaataaag cggatgagc agtttttaa 
      aagatgaat aaatttttt tacaattaa atttcgtct 
      ttctatata tttcaaaaa tatataatt tcggacgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY