Status
Please wait ...
Gene information
General information
TC015329 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 385763..388489
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015329
    iBeetle screen iB_05797

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015329 RA
      MSEFDSSLA IVENGAPKT KRKHTKIVN LMIKLAVFV 
      LINAHFIWA SYYFINNTS DTLDSTTCS GYGLWIIVF 
      IFIYFLTLY SLLLKPYIL PLIDTKKLA PLFKPINRI 
      PHWPLVSRG VVLAAILAF IVYDYRDDF TRLMPLTGL 
      VIFFIIGFL FSPAKKQIP WPTVISGLI AQFVLGLLM 
      IRWETGRNI FSCVGDRVD TFLHYAVNA SAFVYSEEL 
      VLTKAIFAF NALAAIYLM NFCINILYY YGIMQSIVL 
      ELGKILQFV LGTPICESV NSAANIFLG MSESPFLLK 
      PYLDHLTDS EIHSIMTSG FASVSGTVL AAYISFGAS 
      PAHLVTSCV MSAPAALCY SKLMYPETE EVTVTQENI 
      KTMKMKFGS VLDAATKGA NEASQVVIG IIANLISFI 
      SFVYFINGV LGWLGTLVG FIAEDEIWS LELILGKIL 
      IPLSYTMGV EWADCEKVA QLIGMKTIL NEFIAFQKM 
      KTMDLSDKS RIIATYSIC GFANPGSIG TMLSTLTIF 
      MPRKKHSVT KLVFRAFIG GSFACFMTA CIAGLLTV
    CDS sequence
    • >TC015329 RA
      atgtccgaa tttgattca agtttggca attgtggaa 
      aatggcgcg ccgaaaacg aaacgaaaa cacacgaaa 
      attgtcaac ttgatgatc aaacttgca gtttttgta 
      ttaattaac gcacacttt atttgggcc agttactac 
      tttattaac aacacaagt gacacgctt gattcaacc 
      acttgcagt ggctatggc ctctggata attgttttt 
      atttttata tatttctta actctctac tctttgctc 
      ttaaaaccg tacattctt cctctcatt gacacaaaa 
      aaattggca ccactgttc aaaccaatc aaccgaatt 
      ccccattgg ccgttggtg tcacgtggt gtcgtcttg 
      gcggccatc ttggccttc atagtgtat gactatcgt 
      gacgatttc acccgtttg atgcctttg acaggtcta 
      gtgattttt tttatcata ggttttcta ttctcacca 
      gccaagaaa caaatacca tggccgact gtgatttct 
      ggacttatt gcccagttt gtcttaggc ctcttgatg 
      attcgatgg gaaactggt cgtaatatt ttcagttgc 
      gttggtgac agagttgac actttcctg cattacgct 
      gtcaatgcg tcggcattt gtctacagc gaagagttg 
      gtcttgacc aaagccatc tttgcgttt aacgcactc 
      gccgccatt tatttaatg aacttctgc attaatatt 
      ctatactat tatggaata atgcaaagt atcgtcctt 
      gagttagga aagattttg caatttgtg ttgggaaca 
      ccgatttgc gaaagtgtg aacagtgcc gccaacata 
      tttctcgga atgagcgag tcgccattt ttactgaag 
      ccatatttg gatcacttg accgattcg gaaattcat 
      tccattatg acatccggt tttgcgtca gtttccggt 
      actgttttg gcggcctat atttcattt ggggcaagt 
      ccggcccat ttggtcact tcgtgtgtc atgtcagcg 
      cctgctgcg ctctgttac agtaaacta atgtatccg 
      gaaacggaa gaagtgaca gtcacacaa gaaaatatc 
      aaaactatg aaaatgaaa tttggttca gtgttggac 
      gctgcgact aaaggggcc aacgaagcg tcgcaagtt 
      gtcattggc attattgcc aacttaatc tcatttata 
      tcatttgtt tattttatt aatggtgtc ctgggctgg 
      ttggggaca ctagttggt ttcatagct gaggacgaa 
      atatggtca ctagaactg attttgggg aaaatctta 
      attccccta agttacaca atgggtgtg gagtgggca 
      gattgcgaa aaggtggct caattaatt ggaatgaaa 
      acaatactc aacgaattc atcgcattt caaaaaatg 
      aaaacaatg gacttgagt gacaagagt cgtatcata 
      gcaacgtac tcaatttgc ggttttgcc aacccaggt 
      tccataggc actatgttg tcaacactg acgattttc 
      atgccacgg aaaaaacac agtgttacc aaactagtt 
      tttagggct tttatcggc ggctcgttc gcctgtttt 
      atgacagct tgtattgcg ggacttttg acagtgtag
    mRNA sequence
    • >TC015329 RA
      aattagaac gcagattag aatctttcg tattttttt 
      tgctaaata aattttcgt aatcataat tataaaaaa 
      aaatcatta atttcacat agcgtgacg cgaaataac 
      cggaattac aggtgtatc acgtgactg gcatatttc 
      gccagaaat tttgccgga tctgagtca agtgaattc 
      tcctcgtgt aatcgcaat tattttatc acttatctg 
      aactattga ggaggagga aaacagtta ttagttttg 
      tgttgtagt gtccagtga aaatgtccg aatttgatt 
      caagtttgg caattgtgg aaaatggcg cgccgaaaa 
      cgaaacgaa aacacacga aaattgtca acttgatga 
      tcaaacttg cagtttttg tattaatta acgcacact 
      ttatttggg ccagttact actttatta acaacacaa 
      gtgacacgc ttgattcaa ccacttgca gtggctatg 
      gcctctgga taattgttt ttattttta tatatttct 
      taactctct actctttgc tcttaaaac cgtacattc 
      ttcctctca ttgacacaa aaaaattgg caccactgt 
      tcaaaccaa tcaaccgaa ttccccatt ggccgttgg 
      tgtcacgtg gtgtcgtct tggcggcca tcttggcct 
      tcatagtgt atgactatc gtgacgatt tcacccgtt 
      tgatgcctt tgacaggtc tagtgattt tttttatca 
      taggttttc tattctcac cagccaaga aacaaatac 
      catggccga ctgtgattt ctggactta ttgcccagt 
      ttgtcttag gcctcttga tgattcgat gggaaactg 
      gtcgtaata ttttcagtt gcgttggtg acagagttg 
      acactttcc tgcattacg ctgtcaatg cgtcggcat 
      ttgtctaca gcgaagagt tggtcttga ccaaagcca 
      tctttgcgt ttaacgcac tcgccgcca tttatttaa 
      tgaacttct gcattaata ttctatact attatggaa 
      taatgcaaa gtatcgtcc ttgagttag gaaagattt 
      tgcaatttg tgttgggaa caccgattt gcgaaagtg 
      tgaacagtg ccgccaaca tatttctcg gaatgagcg 
      agtcgccat ttttactga agccatatt tggatcact 
      tgaccgatt cggaaattc attccatta tgacatccg 
      gttttgcgt cagtttccg gtactgttt tggcggcct 
      atatttcat ttggggcaa gtccggccc atttggtca 
      cttcgtgtg tcatgtcag cgcctgctg cgctctgtt 
      acagtaaac taatgtatc cggaaacgg aagaagtga 
      cagtcacac aagaaaata tcaaaacta tgaaaatga 
      aatttggtt cagtgttgg acgctgcga ctaaagggg 
      ccaacgaag cgtcgcaag ttgtcattg gcattattg 
      ccaacttaa tctcattta tatcatttg tttatttta 
      ttaatggtg tcctgggct ggttgggga cactagttg 
      gtttcatag ctgaggacg aaatatggt cactagaac 
      tgattttgg ggaaaatct taattcccc taagttaca 
      caatgggtg tggagtggg cagattgcg aaaaggtgg 
      ctcaattaa ttggaatga aaacaatac tcaacgaat 
      tcatcgcat ttcaaaaaa tgaaaacaa tggacttga 
      gtgacaaga gtcgtatca tagcaacgt actcaattt 
      gcggttttg ccaacccag gttccatag gcactatgt 
      tgtcaacac tgacgattt tcatgccac ggaaaaaac 
      acagtgtta ccaaactag tttttaggg cttttatcg 
      gcggctcgt tcgcctgtt ttatgacag cttgtattg 
      cgggacttt tgacagtgt agccaaccg tacgaataa 
      aatcgctat ttttctagt gttttgttt tattttttc 
      acctttcca ttaaaaaaa tcgtatttg aaaaaattt 
      tgttttccc aaaaattgt taaaatggc ctaaaaaca 
      ttattagtg taatcaaca attcaaatt tgacaatct 
      taagcttaa ataaaacgt tgagaccta a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY