Status
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Gene information
General information
TC015327 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 409223..419476
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015327
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature lateral pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015327 RA
      MEGDAYQQY CYQGSDSWY NSGVTPMKQ IEACLQSAG 
      KDRKAYSPL AEAESLLEP PDPDNNNRH STHNKNHSL 
      HQSICPSLS NAGAILEMK HLWDEFHSL GTEMIVTKA 
      GRRMFPTFQ VKLCGLDLH AEYMLMMDF VPVDDKRYR 
      YAFHSSSWV VAGKADPVS PPRIHVHPD SPATGAQWM 
      KQTVSFDKL KLTNNQLDD NGHIILNSM HRYQPRFHI 
      VYLPSKNTT TATEENCSE NFKTFVFPE TAFTAVTAY 
      QNHRITQLK IASNPFAKG FRDCDPDET SPEPQSSQV 
      QRQRPTSRS TPTTKDGEE IAAPEPEAA PMYNPQNRY 
      HLQSNILTT IGQPYTGSD FNYGPVYHP HNLLHNYNT 
      VYSNDKTMR GHNFPRTMY SNYSGFYPN SGNYRSPAL 
      HQNGYDFGP R
    CDS sequence
    • >TC015327 RA
      atggaaggc gatgcctac caacagtat tgttatcaa 
      gggagtgat agttggtac aattcgggt gtgacgccc 
      atgaaacag atcgaggct tgccttcaa agcgccggc 
      aaagaccgc aaagcctac agccccctg gccgaggcc 
      gagtcgctc ctcgaaccc ccagacccc gacaacaac 
      aaccgccac tcaacccac aacaaaaac cacagttta 
      caccaatca atttgtccc tcattatcc aacgctggc 
      gccatcctg gaaatgaaa catttatgg gacgaattc 
      cactcgttg ggcaccgaa atgatcgtc accaaagcc 
      ggccgaaga atgtttccg acgtttcaa gtgaaactg 
      tgcggcctg gacttgcat gccgagtac atgctgatg 
      atggacttt gttccggtc gatgataag aggtacaga 
      tatgccttc cacagctcc agctgggtg gtggcaggg 
      aaggcagac cccgtgtcg ccccccagg atacacgtg 
      cacccagac agccccgcc acgggggcg cagtggatg 
      aaacaaacc gtgtccttt gataagctc aagctgacc 
      aataaccaa ctagatgat aatggacat ataatttta 
      aattcaatg catcgctac caacctcgt ttccacatc 
      gtctacctc ccttcaaaa aacaccacc accgccacc 
      gaagagaac tgtagtgaa aatttcaag acttttgta 
      ttcccagaa acagcattc acggctgtc acagcctac 
      cagaaccac aggataact caattgaaa atcgccagc 
      aatcccttc gccaagggc tttcgtgac tgcgacccc 
      gacgaaaca agcccagaa ccacaatca tcccaagtg 
      caacgtcaa cgcccaacg tcccgctcc actcccacc 
      accaaagat ggcgaggag atcgccgcc cccgagccg 
      gaggcggcg ccgatgtac aacccccag aaccgatac 
      cacctccag tcgaacatc ctcacgacg attggacag 
      ccctacact ggcagtgat ttcaactac ggccctgtg 
      taccacccc cacaacctc ctccataat tacaacaca 
      gtttatagt aatgacaaa acaatgcga ggacacaat 
      ttcccaagg acgatgtac tccaattat tcggggttt 
      tacccgaac tcggggaat tacaggagt ccggccctg 
      caccaaaac ggctacgac ttcgggccc cgataa
    mRNA sequence
    • >TC015327 RA
      cgaaaaaat gcacttttg cgccgaaaa tatttcacc 
      caacacatg gcccataaa tcatccatt tcactcaac 
      gtgcttcat ttaagttgg ccgtgaaaa atgaaatgc 
      tagttttca cgtcgattt tcctttcgg tgaaataat 
      tttcccgag gtgtcgaaa aatcgattt catcgctag 
      tgccgtgat caaattaag agtgtcgac gttcaactg 
      ttggcgagt taaatcgcg gataactag atcgcttga 
      attaattgg gtttttgaa ctggtggct aatcatgga 
      aggcgatgc ctaccaaca gtattgtta tcaagggag 
      tgatagttg gtacaattc gggtgtgac gcccatgaa 
      acagatcga ggcttgcct tcaaagcgc cggcaaaga 
      ccgcaaagc ctacagccc cctggccga ggccgagtc 
      gctcctcga acccccaga ccccgacaa caacaaccg 
      ccactcaac ccacaacaa aaaccacag tttacacca 
      atcaatttg tccctcatt atccaacgc tggcgccat 
      cctggaaat gaaacattt atgggacga attccactc 
      gttgggcac cgaaatgat cgtcaccaa agccggccg 
      aagaatgtt tccgacgtt tcaagtgaa actgtgcgg 
      cctggactt gcatgccga gtacatgct gatgatgga 
      ctttgttcc ggtcgatga taagaggta cagatatgc 
      cttccacag ctccagctg ggtggtggc agggaaggc 
      agaccccgt gtcgccccc caggataca cgtgcaccc 
      agacagccc cgccacggg ggcgcagtg gatgaaaca 
      aaccgtgtc ctttgataa gctcaagct gaccaataa 
      ccaactaga tgataatgg acatataat tttaaattc 
      aatgcatcg ctaccaacc tcgtttcca catcgtcta 
      cctcccttc aaaaaacac caccaccgc caccgaaga 
      gaactgtag tgaaaattt caagacttt tgtattccc 
      agaaacagc attcacggc tgtcacagc ctaccagaa 
      ccacaggat aactcaatt gaaaatcgc cagcaatcc 
      cttcgccaa gggctttcg tgactgcga ccccgacga 
      aacaagccc agaaccaca atcatccca agtgcaacg 
      tcaacgccc aacgtcccg ctccactcc caccaccaa 
      agatggcga ggagatcgc cgcccccga gccggaggc 
      ggcgccgat gtacaaccc ccagaaccg ataccacct 
      ccagtcgaa catcctcac gacgattgg acagcccta 
      cactggcag tgatttcaa ctacggccc tgtgtacca 
      cccccacaa cctcctcca taattacaa cacagttta 
      tagtaatga caaaacaat gcgaggaca caatttccc 
      aaggacgat gtactccaa ttattcggg gttttaccc 
      gaactcggg gaattacag gagtccggc cctgcacca 
      aaacggcta cgacttcgg gccccgata attattatt 
      ccgcactat ttatatcca ataaaacta ttttatttt 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY