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Gene information
General information
TC015326 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 421206..424564
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015326
    iBeetle screen iB_05795

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015326 RA
      MAGPTLQAL KIYWKSLFM VLFPIALLP IFLTNNIAE 
      MRCLYVVCL MAGFWVLEI LPSGVTGLF PIIFFPIMG 
      ILDTRTTTM SYFKESNMM FIGGLIIAL AIEYCNLHT 
      RISLRAIKM IGCSYRRLH FGLVTLTML VSMWISNTA 
      ATAMMLPII QAVLEELEA QGLGKMYEG EVDEEGQFD 
      ESTKRPTRV TMCFYIGTA YAATIGGLG CIVGSGTNL 
      VLKGIYETE FPKSNGVEF NKWLAANVP LMLAIMYPT 
      WIWMQFWFM GLWRPNSED AKAIDVGKE GEKITRQVL 
      TDKLAEMGL FTIHEVLVG ILFVMAALL WFLRRPGFM 
      KGWPDLITN TATGDATAA MIVVLLLFI IPARYDCTR 
      EISLKLITW QYVQKKMHW SLIFLMGGG FAIADGMKA 
      SGLGKMVAN ELNAVTTYP KLVIMVVGC LIGAFVTQF 
      TSNAAVANI LLPIVASLA RKAKIYPMF LMMPVNLSC 
      SFAFCLPVA TPPNAIVAM SCNVKTTEM IKVGLLVEV 
      IALVMLLLI FPNLGSVIW DFNSFPDWA KKD
    CDS sequence
    • >TC015326 RA
      atggcgggg ccaacgctc caggccctc aaaatctac 
      tggaagagc ctcttcatg gtgctcttc cccatagcg 
      ctgctcccc atcttcctg acaaataat atcgctgaa 
      atgcgctgt ctgtacgtg gtttgtttg atggccggg 
      ttctgggtg ttggagatt cttccctcg ggagttact 
      gggctgttt cccattatt ttcttcccg attatgggg 
      attttggac accaggact acgaccatg agctacttc 
      aaggagtcg aacatgatg tttattggt ggcttgatt 
      attgcactt gctattgag tattgtaat ttgcatacg 
      aggatttcg ctacgggcg atcaaaatg attgggtgc 
      agttatcgc cgtttgcac ttcggtcta gtcacctta 
      accatgttg gtatcaatg tggatatca aacactgca 
      gccactgca atgatgttg cccataatt caggcagtt 
      ttggaagaa ctggaagcc caaggtctt ggaaaaatg 
      tacgaaggt gaagttgat gaagaagga caatttgat 
      gaatccacc aaacgtcca acaagagtt acgatgtgt 
      ttctatatt ggtacggca tatgcagcc accattgga 
      ggattggga tgtattgtg ggcagtggt accaaccta 
      gtactcaaa ggcatttac gagacagaa ttccctaaa 
      agcaacggt gttgaattt aacaaatgg ttggctgca 
      aatgtgcct ttaatgttg gcaataatg tacccaaca 
      tggatttgg atgcaattc tggttcatg ggcttatgg 
      cgtccgaac tcagaggac gccaaagcc atagatgtg 
      ggaaaagag ggtgaaaaa attacaaga caagtatta 
      actgataaa ttggccgaa atggggctt ttcaccatc 
      catgaagta ctagtaggg attttgttt gttatggct 
      gcgctttta tggtttttg agacggcct gggttcatg 
      aaaggctgg cctgacctt atcacaaac actgctact 
      ggtgacgcc acagccgcc atgattgtc gtcctcctc 
      cttttcatc atcccagcc cgctacgat tgcactcgc 
      gaaatttct ctcaaatta ataacatgg caatacgtt 
      cagaaaaaa atgcattgg agtttaatt tttctgatg 
      ggtggtggt tttgccatc gctgatggt atgaaagca 
      agtggtttg ggcaaaatg gtagcgaat gaattgaac 
      gctgtaacc acttatccg aaattggtg ataatggtt 
      gttgggtgt ttgattgga gcttttgtc acccaattt 
      acgagtaat gcagctgtg gccaatata cttttgccc 
      atcgttgcc agtttagca agaaaagct aaaatttat 
      ccaatgttt ctgatgatg ccggtgaat ttgtcgtgt 
      tcgttcgcc ttttgtctt ccagtggcc acgccccca 
      aacgccatt gttgccatg tcgtgcaat gtgaaaacg 
      accgaaatg atcaaagtt ggcctgttg gtggaagtc 
      atcgcactt gtgatgctg ctccttatt tttccgaat 
      ctaggttcg gttatttgg gattttaac agttttccc 
      gattgggcc aaaaaggac tga
    mRNA sequence
    • >TC015326 RA
      aaaaattta caaaaatcg ccaagtttg cgttttttc 
      tagttttgt cacgtgtca aattgcaat ggcggggcc 
      aacgctcca ggccctcaa aatctactg gaagagcct 
      cttcatggt gctcttccc catagcgct gctccccat 
      cttcctgac aaataatat cgctgaaat gcgctgtct 
      gtacgtggt ttgtttgat ggccgggtt ctgggtgtt 
      ggagattct tccctcggg agttactgg gctgtttcc 
      cattatttt cttcccgat tatggggat tttggacac 
      caggactac gaccatgag ctacttcaa ggagtcgaa 
      catgatgtt tattggtgg cttgattat tgcacttgc 
      tattgagta ttgtaattt gcatacgag gatttcgct 
      acgggcgat caaaatgat tgggtgcag ttatcgccg 
      tttgcactt cggtctagt caccttaac catgttggt 
      atcaatgtg gatatcaaa cactgcagc cactgcaat 
      gatgttgcc cataattca ggcagtttt ggaagaact 
      ggaagccca aggtcttgg aaaaatgta cgaaggtga 
      agttgatga agaaggaca atttgatga atccaccaa 
      acgtccaac aagagttac gatgtgttt ctatattgg 
      tacggcata tgcagccac cattggagg attgggatg 
      tattgtggg cagtggtac caacctagt actcaaagg 
      catttacga gacagaatt ccctaaaag caacggtgt 
      tgaatttaa caaatggtt ggctgcaaa tgtgccttt 
      aatgttggc aataatgta cccaacatg gatttggat 
      gcaattctg gttcatggg cttatggcg tccgaactc 
      agaggacgc caaagccat agatgtggg aaaagaggg 
      tgaaaaaat tacaagaca agtattaac tgataaatt 
      ggccgaaat ggggctttt caccatcca tgaagtact 
      agtagggat tttgtttgt tatggctgc gcttttatg 
      gtttttgag acggcctgg gttcatgaa aggctggcc 
      tgaccttat cacaaacac tgctactgg tgacgccac 
      agccgccat gattgtcgt cctcctcct tttcatcat 
      cccagcccg ctacgattg cactcgcga aatttctct 
      caaattaat aacatggca atacgttca gaaaaaaat 
      gcattggag tttaatttt tctgatggg tggtggttt 
      tgccatcgc tgatggtat gaaagcaag tggtttggg 
      caaaatggt agcgaatga attgaacgc tgtaaccac 
      ttatccgaa attggtgat aatggttgt tgggtgttt 
      gattggagc ttttgtcac ccaatttac gagtaatgc 
      agctgtggc caatatact tttgcccat cgttgccag 
      tttagcaag aaaagctaa aatttatcc aatgtttct 
      gatgatgcc ggtgaattt gtcgtgttc gttcgcctt 
      ttgtcttcc agtggccac gcccccaaa cgccattgt 
      tgccatgtc gtgcaatgt gaaaacgac cgaaatgat 
      caaagttgg cctgttggt ggaagtcat cgcacttgt 
      gatgctgct ccttatttt tccgaatct aggttcggt 
      tatttggga ttttaacag ttttcccga ttgggccaa 
      aaaggactg aataaactt gcaataaat ttttgaatc 
      aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY