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Gene information
General information
TC015308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 655842..661393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015308
    iBeetle screen iB_05794

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015308 RA
      MAESLLNQA LAPDFAPEP FIQWLTSPE LQGAQSTAY 
      SCSRTDFIT FFLNYVHEE IGSAISERK PEPLDTSKS 
      STELGPHDL NTSVCNSTP KGGSDSSVL SYMSPISPI 
      CLGDFFVNK RAPPRKTKK RIKPTNLTL NSSSFHKSD 
      NSFNFTSES EEFPSGRSF LLEERLKVD FRSPKTVIP 
      GGEVLPELG LVTNRPQID RIVQLYSLL IADNCVLNL 
      TREIYFLIT LLLKKQFYN NDWGINVLS DTITGFLFN 
      SIHNVVYFA SKSLENLVG VLRNFDNST VRLLTENKR 
      LREFAPTLI AQLEQTVKP DRVLISLEN LQNNICFNS 
      DTDNRNNFP NDLSFHAFK KQRDLFYDI LRVWEQHHL 
      APGFSYTVS LGGKIKTLL SLSLEPTNF VHFARLFKG 
      QLLTCRVCD DLIITSLDV DAQKLNKLK NRCVTKESN 
      QGLNSLPKF SESEEFFRD FIVVGANFA FNRHLADAF 
      VEEIVELNE SYFGNAEED ENSVDLVTK KSYFSCVKS 
      LRILAKFLG FVESLPYKS ECGNYSEQL LTTHLKIRK 
      QICPTFDVK TLLENSIRR KSVILCVPW LIKYLSMLD 
      TVTLKLPYY TQIQNTLYW IYRNFNWGP SPNIYLIKF 
      CLGWLFELP HIPDNNALY FTTEETTQN STESPKTSY 
      IDQLEIVDQ DILYFCCPY LNEIKKLLT TTTTNQMIT 
      IKHITPVTA QQSPDEIAR KKLEQQLEE AFFNGQPIS 
      LRKSVEFVS ERVASTCVK YVCSVLVPE FKKKSLETF 
      KSMISTIDD ETVLKQKAN QFATDCLNE LQVACEREI 
      KKITQNKIR NGIESLLAM DVLPQTRDV CVGITEKMC 
      LERVRQWLE AHVTSVIFS KDFNDEVQK LTNQETKRT 
      KPLFVLPPG GSKRQHNDR TISGAHLVT KFQSLTIDI 
      IEGKNVHNQ LISLLDATS DSINERGDF NQCVVATLC 
      TQILDVVIL LLGSGVLDG VLSKSCDIW RLYYRDDLF 
      KSLLSPRNV IILGRSKAA WDAFAQFCA LLLKQKLLT 
      CDSFEAQCT GIFRTDWDQ VTLKVISDF FKRFHTLYR 
      EQGGSVSDF TFLLDFFSD YCEDLV
    CDS sequence
    • >TC015308 RA
      atggctgaa agcctccta aatcaagca ctagccccc 
      gacttcgct cccgagccc ttcatccag tggctcacg 
      agcccggaa ttgcagggg gcccaaagc accgcctac 
      tcctgctcc cgcaccgat ttcatcact ttcttcctc 
      aactacgtg cacgaggag atcggtagc gcgatcagc 
      gagcgcaag ccggagccc ctcgacacc agcaaaagt 
      agcaccgag ctgggcccc catgacttg aacacgagt 
      gtctgtaac agcaccccg aaagggggc tccgactcg 
      agcgtcctg agctacatg agccccatt tcgcccatc 
      tgtttgggc gattttttc gtcaacaag cgtgcgccc 
      cctcgtaaa acgaaaaag cgaatcaaa ccaacgaat 
      ttaacgctg aatagttca agcttccac aagtcggac 
      aatagtttt aattttact agcgagagt gaggagttt 
      ccaagtggg cgctcgttc ctgctagag gagcgcctg 
      aaagttgac tttcggagc cccaagact gttataccg 
      gggggcgaa gtcctgcca gagctgggc ctggtgacc 
      aatcgcccc caaatcgac cgaattgtc caactttac 
      tcgctttta atcgccgat aattgcgtc ctaaacctc 
      acacgcgaa atatatttt ctcatcacg ttgcttttg 
      aaaaaacaa ttttacaac aatgactgg ggcattaac 
      gtgttaagt gacacaatc accggtttt ttgttcaat 
      tcaattcac aacgtagtg tattttgcc agcaaatcg 
      ctcgagaat ctagtaggc gttttgcga aattttgac 
      aattcaacg gttcggtta ctaaccgaa aacaagcga 
      ctgcgcgaa ttcgcgcca actctgatc gcacaattg 
      gaacagacc gtgaaacct gaccgggtg ttaatttcg 
      cttgaaaat ttgcaaaat aatatttgt tttaattcg 
      gacaccgat aaccgtaat aattttccg aacgacttg 
      tcgtttcac gcgtttaaa aagcagcgc gatttgttt 
      tacgatatt ttgcgcgtg tgggagcag caccatcta 
      gcgcccggt tttagttac acggtgagt cttggcggg 
      aaaattaaa acgcttttg agtttgtcg ctggagccg 
      acgaatttt gtgcatttc gcgcggtta ttcaaaggt 
      caattgttg acgtgtcgc gtttgcgac gacttgatt 
      attacgagt ttggacgtt gatgcgcaa aaattgaac 
      aaattgaag aatcgctgc gtgacgaag gagtcaaat 
      caggggctg aattcgttg ccgaagttt agcgagagt 
      gaggagttt tttcgggat tttatcgtg gttggggcg 
      aattttgcg tttaatagg catctggcc gatgcgttt 
      gttgaggag attgtcgag ttgaatgag agttatttt 
      gggaatgct gaggaagat gagaatagt gtggatttg 
      gtgacgaaa aaatcgtac ttttcgtgt gttaagtcg 
      ttgcggatt ttggccaag tttttgggg tttgttgag 
      tctttgccg tataaaagt gagtgtggg aattattca 
      gagcaattg ttgaccaca catttgaag atacggaag 
      cagatttgt ccaactttc gacgtcaaa acacttctt 
      gaaaactca atacgtcgc aaatctgtc attttatgc 
      gtcccttgg ctcattaaa tacctctca atgcttgat 
      acagtcacc ttaaaactc ccctactac acccaaatc 
      cagaacact ctttactgg atttatcgt aacttcaac 
      tggggtcct tcccccaac atatacctc atcaaattc 
      tgtcttggt tggttgttt gaactaccg cacataccc 
      gacaacaac gccctctat ttcacaacg gaagaaaca 
      acacaaaat agtacagaa tcgccaaaa acttcctac 
      atcgatcaa ttggaaatc gtcgatcag gacatttta 
      tatttctgt tgtccgtat ttaaacgaa atcaagaag 
      ctcctgacc acgacaaca accaatcaa atgatcaca 
      attaaacac ataaccccg gttaccgcg caacaatcg 
      ccggacgaa atcgcccga aaaaaactc gaacaacaa 
      ctcgaggag gcttttttc aatggacaa ccgatttcg 
      ttgcgtaaa agtgtcgaa tttgtgagc gaacgcgtg 
      gcttcgact tgtgttaaa tacgtgtgt agtgttcta 
      gtgcctgaa tttaaaaaa aaatcactc gaaactttc 
      aaatcaatg atcagtaca atcgatgat gaaactgtt 
      ttaaaacaa aaagccaat cagttcgcc accgattgc 
      ttaaacgaa ctgcaagtc gcttgtgag cgtgaaatt 
      aaaaaaatc acacaaaat aaaatccgc aatggaatt 
      gaatcgtta cttgcgatg gacgttttg ccacaaacg 
      cgcgacgtt tgtgtggga atcaccgaa aaaatgtgt 
      ctcgaaaga gtgcggcag tggctagag gcacacgtc 
      acgtctgtt attttcagt aaggatttc aacgacgag 
      gtgcaaaaa ttgaccaat caggagacg aaacgaacg 
      aaaccactg tttgtgctg ccgcctggc ggctcgaag 
      cggcaacac aatgacagg acgatcagt ggggcccat 
      ttagttaca aaatttcag tcactaact attgatata 
      atcgagggt aaaaacgtc cataatcaa ttgataagt 
      ttattggac gccaccagt gatagtatc aatgaacgt 
      ggtgacttc aaccagtgt gtggtggca acactgtgc 
      acccaaata ctggacgtt gtgatttta ctacttggc 
      agtggtgtt ttagatggc gtcctgtca aagtcatgt 
      gacatttgg cggttgtat taccgcgat gatttgttt 
      aaatcgttg ctatcgcca cgaaatgtc atcattttg 
      gggcgatcc aaagccgcc tgggacgct ttcgcgcag 
      ttttgcgcc cttttactc aaacaaaag ttactaact 
      tgtgatagt ttcgaggca cagtgtacg gggattttt 
      cggacggat tgggatcag gtgaccctg aaggttatt 
      agcgatttt ttcaaacgt ttccacacg ttgtatcgt 
      gagcaaggc ggaagtgtg tcagatttt acgttttta 
      ttggacttt ttctccgat tattgtgaa gatttggtt 
      taa
    mRNA sequence
    • >TC015308 RA
      acaaaaact gtttattgt tcgcgcatg cgcagttgc 
      gaaacccgc gcaatgtta gcacaactt ccaaacgaa 
      attaagcaa aaaccatgg ctgaaagcc tcctaaatc 
      aagcactag cccccgact tcgctcccg agcccttca 
      tccagtggc tcacgagcc cggaattgc agggggccc 
      aaagcaccg cctactcct gctcccgca ccgatttca 
      tcactttct tcctcaact acgtgcacg aggagatcg 
      gtagcgcga tcagcgagc gcaagccgg agcccctcg 
      acaccagca aaagtagca ccgagctgg gcccccatg 
      acttgaaca cgagtgtct gtaacagca ccccgaaag 
      ggggctccg actcgagcg tcctgagct acatgagcc 
      ccatttcgc ccatctgtt tgggcgatt ttttcgtca 
      acaagcgtg cgccccctc gtaaaacga aaaagcgaa 
      tcaaaccaa cgaatttaa cgctgaata gttcaagct 
      tccacaagt cggacaata gttttaatt ttactagcg 
      agagtgagg agtttccaa gtgggcgct cgttcctgc 
      tagaggagc gcctgaaag ttgactttc ggagcccca 
      agactgtta taccggggg gcgaagtcc tgccagagc 
      tgggcctgg tgaccaatc gcccccaaa tcgaccgaa 
      ttgtccaac tttactcgc ttttaatcg ccgataatt 
      gcgtcctaa acctcacac gcgaaatat attttctca 
      tcacgttgc ttttgaaaa aacaatttt acaacaatg 
      actggggca ttaacgtgt taagtgaca caatcaccg 
      gttttttgt tcaattcaa ttcacaacg tagtgtatt 
      ttgccagca aatcgctcg agaatctag taggcgttt 
      tgcgaaatt ttgacaatt caacggttc ggttactaa 
      ccgaaaaca agcgactgc gcgaattcg cgccaactc 
      tgatcgcac aattggaac agaccgtga aacctgacc 
      gggtgttaa tttcgcttg aaaatttgc aaaataata 
      tttgtttta attcggaca ccgataacc gtaataatt 
      ttccgaacg acttgtcgt ttcacgcgt ttaaaaagc 
      agcgcgatt tgttttacg atattttgc gcgtgtggg 
      agcagcacc atctagcgc ccggtttta gttacacgg 
      tgagtcttg gcgggaaaa ttaaaacgc ttttgagtt 
      tgtcgctgg agccgacga attttgtgc atttcgcgc 
      ggttattca aaggtcaat tgttgacgt gtcgcgttt 
      gcgacgact tgattatta cgagtttgg acgttgatg 
      cgcaaaaat tgaacaaat tgaagaatc gctgcgtga 
      cgaaggagt caaatcagg ggctgaatt cgttgccga 
      agtttagcg agagtgagg agttttttc gggatttta 
      tcgtggttg gggcgaatt ttgcgttta ataggcatc 
      tggccgatg cgtttgttg aggagattg tcgagttga 
      atgagagtt attttggga atgctgagg aagatgaga 
      atagtgtgg atttggtga cgaaaaaat cgtactttt 
      cgtgtgtta agtcgttgc ggattttgg ccaagtttt 
      tggggtttg ttgagtctt tgccgtata aaagtgagt 
      gtgggaatt attcagagc aattgttga ccacacatt 
      tgaagatac ggaagcaga tttgtccaa ctttcgacg 
      tcaaaacac ttcttgaaa actcaatac gtcgcaaat 
      ctgtcattt tatgcgtcc cttggctca ttaaatacc 
      tctcaatgc ttgatacag tcaccttaa aactcccct 
      actacaccc aaatccaga acactcttt actggattt 
      atcgtaact tcaactggg gtccttccc ccaacatat 
      acctcatca aattctgtc ttggttggt tgtttgaac 
      taccgcaca tacccgaca acaacgccc tctatttca 
      caacggaag aaacaacac aaaatagta cagaatcgc 
      caaaaactt cctacatcg atcaattgg aaatcgtcg 
      atcaggaca ttttatatt tctgttgtc cgtatttaa 
      acgaaatca agaagctcc tgaccacga caacaacca 
      atcaaatga tcacaatta aacacataa ccccggtta 
      ccgcgcaac aatcgccgg acgaaatcg cccgaaaaa 
      aactcgaac aacaactcg aggaggctt ttttcaatg 
      gacaaccga tttcgttgc gtaaaagtg tcgaatttg 
      tgagcgaac gcgtggctt cgacttgtg ttaaatacg 
      tgtgtagtg ttctagtgc ctgaattta aaaaaaaat 
      cactcgaaa ctttcaaat caatgatca gtacaatcg 
      atgatgaaa ctgttttaa aacaaaaag ccaatcagt 
      tcgccaccg attgcttaa acgaactgc aagtcgctt 
      gtgagcgtg aaattaaaa aaatcacac aaaataaaa 
      tccgcaatg gaattgaat cgttacttg cgatggacg 
      ttttgccac aaacgcgcg acgtttgtg tgggaatca 
      ccgaaaaaa tgtgtctcg aaagagtgc ggcagtggc 
      tagaggcac acgtcacgt ctgttattt tcagtaagg 
      atttcaacg acgaggtgc aaaaattga ccaatcagg 
      agacgaaac gaacgaaac cactgtttg tgctgccgc 
      ctggcggct cgaagcggc aacacaatg acaggacga 
      tcagtgggg cccatttag ttacaaaat ttcagtcac 
      taactattg atataatcg agggtaaaa acgtccata 
      atcaattga taagtttat tggacgcca ccagtgata 
      gtatcaatg aacgtggtg acttcaacc agtgtgtgg 
      tggcaacac tgtgcaccc aaatactgg acgttgtga 
      ttttactac ttggcagtg gtgttttag atggcgtcc 
      tgtcaaagt catgtgaca tttggcggt tgtattacc 
      gcgatgatt tgtttaaat cgttgctat cgccacgaa 
      atgtcatca ttttggggc gatccaaag ccgcctggg 
      acgctttcg cgcagtttt gcgcccttt tactcaaac 
      aaaagttac taacttgtg atagtttcg aggcacagt 
      gtacgggga tttttcgga cggattggg atcaggtga 
      ccctgaagg ttattagcg attttttca aacgtttcc 
      acacgttgt atcgtgagc aaggcggaa gtgtgtcag 
      attttacgt ttttattgg actttttct ccgattatt 
      gtgaagatt tggtttaat aaaacacaa ttttaagag 
      agttttatt tgagttggc acccctgtg gctcatctg 
      agcggtcca aacagtgga aagtcccgt cgcagaaat 
      tttctttca ttatacaat cgccgttaa ggcatggga 
      tggttccat tgggacaat ctaacagga aaaatttaa 
      taaaaaaat aaaaataaa
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