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Gene information
General information
TC034720 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 793578..798463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034720
    iBeetle screen iB_05793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034720 RA
      MLPQLFLAV FAASVCSQT IPENPCPEF FTYRQERGV 
      FFGEINVPY DGSKNLNLA VNISMVGLY QNLKLRLEL 
      LTPADQILS AQYLKYRVN FPFQNVVPR ITQIAFNGR 
      IFCYGSSEQ VSMKGVTNL WSNIVYRIN QYTYNIQSG 
      FKEPISSNN LNQYNNEPM NNPFIHPTT ETTTTTTTT 
      CPTIQDNCG IANDIQTLV LKGEKTIEN EYPWLVAMF 
      HRQGVSYEF QCTGNLITD RHVLTAGHC VWYYKAPLI 
      DKSDILLVL GRSDISHWA SAGALIRTA SQVTPHPNY 
      KQYSGHCDL AIIKMNEEV IFKPTIRPI CLWTGDTDL 
      KTFAGVRGV VAGWGKSSE GRHVVATPR KVAMPAVSQ 
      ETCLRSHAN FRNLTSDMT FCAGNRDGS GPCNGDSGA 
      GFMVKKEGR WYLRGVVST AIKKEDFSC DLNEFVVFS 
      DVGKLREWV KGVVGAGLL K
    CDS sequence
    • >TC034720 RA
      atgttgcca cagctgttc ttagccgtg tttgctgcg 
      agtgtctgc agtcagacg atcccggaa aatccgtgt 
      ccggaattt ttcacgtac cgacaagaa aggggtgtg 
      tttttcggc gagattaat gttccgtac gatggaagc 
      aagaatctc aacttggct gtgaatatt tccatggtt 
      gggctttat caaaatttg aaattgaga cttgaactt 
      ttgactcct gctgatcaa atactttcg gcacaatat 
      ttgaagtat agggtgaat tttccattt caaaatgtc 
      gtcccgaga attacgcag attgcgttt aatggacgg 
      attttttgc tatggatcc tcagagcaa gtttctatg 
      aaaggtgtg accaatctt tggtccaac atcgtctac 
      cgcatcaac caatacact tataacatc cagagcggg 
      ttcaaggaa ccaatcagc tccaataat ttgaaccaa 
      tacaataat gaacccatg aacaacccc ttcatccac 
      cccaccact gaaaccacc accacaacc accaccaca 
      tgtcccacg attcaagac aactgtgga atagccaac 
      gacatccaa accttggtt ctaaagggt gaaaagaca 
      attgaaaat gaataccca tggttggtt gctatgttc 
      cacagacaa ggggtttcc tacgagttt caatgcacg 
      gggaattta atcacagac cgtcatgta ctcacagct 
      ggccactgc gtctggtac tacaaagcc cccctgatt 
      gacaaatcc gacattctc ctcgtcctc ggccgctcc 
      gacatctcc cattgggcc tccgccgga gccctcata 
      cgcaccgcc tcccaagtc acgccccac cccaactac 
      aagcaatac tccggccac tgcgacctc gcaatcatc 
      aaaatgaac gaagaggtg attttcaag ccaaccatt 
      cgccctatc tgtctctgg acgggcgac accgacctc 
      aaaacattt gccggcgtt aggggcgtg gttgctggg 
      tggggcaaa agctcagag gggcggcac gtcgttgcc 
      acgccccga aaagttgca atgccggcg gtttcgcaa 
      gaaacgtgt ctacgatcg cacgcaaat ttccgaaac 
      ttgacgtcg gatatgacg ttctgtgcg gggaatagg 
      gatggaagt gggccgtgc aatggcgac agtggggcg 
      gggtttatg gtgaagaag gaggggcgg tggtatttg 
      agaggcgtg gtctccacg gcgattaaa aaggaggat 
      ttctcgtgc gatttgaac gagtttgtg gtgttttcg 
      gatgtgggg aagttgagg gagtgggtt aagggcgtg 
      gtaggggcg gggctattg aaataa
    mRNA sequence
    • >TC034720 RA
      taagtccaa aatctgatt ttttccttt agttttgat 
      gtgtaaaca agtgacttc atttggcac atcctcttg 
      taaaaccct caaggctgt gcattattt tacgactta 
      accttggca atgatcggt cgttaatcc ggaagacag 
      atctctgat aactcgctc caaataatc ccaccatgg 
      ttgaaagtc gtatcaaat caagcagtt cttgaaagg 
      acacggcta agaccagtt ggaaaatcc agcacaaaa 
      tgattcaat aaacacaat gttgccaca gctgttctt 
      agccgtgtt tgctgcgag tgtctgcag tcagacgat 
      cccggaaaa tccgtgtcc ggaattttt cacgtaccg 
      acaagaaag gggtgtgtt tttcggcga gattaatgt 
      tccgtacga tggaagcaa gaatctcaa cttggctgt 
      gaatatttc catggttgg gctttatca aaatttgaa 
      attgagact tgaactttt gactcctgc tgatcaaat 
      actttcggc acaatattt gaagtatag ggtgaattt 
      tccatttca aaatgtcgt cccgagaat tacgcagat 
      tgcgtttaa tggacggat tttttgcta tggatcctc 
      agagcaagt ttctatgaa aggtgtgac caatctttg 
      gtccaacat cgtctaccg catcaacca atacactta 
      taacatcca gagcgggtt caaggaacc aatcagctc 
      caataattt gaaccaata caataatga acccatgaa 
      caacccctt catccaccc caccactga aaccaccac 
      cacaaccac caccacatg tcccacgat tcaagacaa 
      ctgtggaat agccaacga catccaaac cttggttct 
      aaagggtga aaagacaat tgaaaatga atacccatg 
      gttggttgc tatgttcca cagacaagg ggtttccta 
      cgagtttca atgcacggg gaatttaat cacagaccg 
      tcatgtact cacagctgg ccactgcgt ctggtacta 
      caaagcccc cctgattga caaatccga cattctcct 
      cgtcctcgg ccgctccga catctccca ttgggcctc 
      cgccggagc cctcatacg caccgcctc ccaagtcac 
      gccccaccc caactacaa gcaatactc cggccactg 
      cgacctcgc aatcatcaa aatgaacga agaggtgat 
      tttcaagcc aaccattcg ccctatctg tctctggac 
      gggcgacac cgacctcaa aacatttgc cggcgttag 
      gggcgtggt tgctgggtg gggcaaaag ctcagaggg 
      gcggcacgt cgttgccac gccccgaaa agttgcaat 
      gccggcggt ttcgcaaga aacgtgtct acgatcgca 
      cgcaaattt ccgaaactt gacgtcgga tatgacgtt 
      ctgtgcggg gaataggga tggaagtgg gccgtgcaa 
      tggcgacag tggggcggg gtttatggt gaagaagga 
      ggggcggtg gtatttgag aggcgtggt ctccacggc 
      gattaaaaa ggaggattt ctcgtgcga tttgaacga 
      gtttgtggt gttttcgga tgtggggaa gttgaggga 
      gtgggttaa gggcgtggt aggggcggg gctattgaa 
      ataacggac ggttttctt gtaatttat tggttgtga 
      cgattatga acttacaat aaaaatttt atttttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY