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Gene information
General information
TC015295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 805573..825969
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015295
    iBeetle screen iB_05792

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015295 RA
      MHILSFLLC VVTTKAMVV RPQLYSSPC PDTFQYEMD 
      QNGQLYGTI GVYSFDENI VRLNVELSV GNRVNSYNG 
      EIQLANPKE LIFDDIQQQ RPIKYKVFF PRWENTPPR 
      VTKISVNGQ VVCSGLPIE MNNWVRVLT KINLEHTLT 
      IKVSPLRSQ SSPFVPSRN NPFLSEKAN PFQDTKVVT 
      KPIRNQFLD EFPAPVRTT TEKIFRSSG PSNELFGGN 
      PFLNGKPTV PPTQPPPPG TCGVSIVAN PLVINGNTV 
      PRGAFPWLT AIFAVTTTG LEYKCSGSL VSQKHIITA 
      AHCVQEGRK RPQPERFLF VLGKLNIKK WSLSEGEKM 
      VEAEDIRIH PDYVPLTSD ADIAVVILA EKIDFSKYI 
      RPICLWSEP DDVDKIVGQ KGKVVGWGR DEQDNLMTA 
      EPKQADIPV VGQEECLRS SEAFRYITS ERTFCAGER 
      NQSGPWRVH SVSPVVPSL PLEKMAAFP DVLARARTK 
      KCAPAGRKL PVFGQSAPE LPNVKIWKK HRRIIVPTL 
      NQSILNTFP QIICQQCDI LLEILEKKC DKGEIDHYK 
      FVTNFAVDV VSETIFGVP LNAQITDAN YSRTFDKIM 
      EVVFMRIFR VDYHSDFLF SWTKEYKAE QENVKLVKE 
      TTRDLIERK KEQITCQLE VEEEKKRPF LDVLVGKYL 
      NEELSYQEL EDEVSTFLL AGSDTNATA GCFVLTLLG 
      MHQDVQEKL YEEIIEVLG PEKYPTLDD LPKLKYTER 
      VIKETLRLF PGAPFIARI ASDDIDLGD YVIPRGSNI 
      AVGYVHLHR SEKYWEEPL KFNPERFLP ENVAKRHPY 
      TWLPFSGGL RNCVGGKFG MMVMKIMIS MIIRKFRVK 
      SSVKSVGDI ELTANIVLK PKNGFRLAF TLR
    CDS sequence
    • >TC015295 RA
      atgcacatc ttgagcttc cttctttgc gtcgtcaca 
      acgaaagcc atggtcgtc agaccccag ctgtactcc 
      agcccatgc ccagacacc ttccagtac gaaatggac 
      cagaatggg cagctgtat ggtactatt ggagtctac 
      tccttcgac gagaacatc gtcagactc aacgtcgag 
      ctgtcggtt ggaaaccgc gtgaacagt tacaatggc 
      gagatccag ttagccaat cccaaagaa ctcatcttc 
      gacgacatc cagcaacaa agacccatc aaatataaa 
      gttttcttc cctcgatgg gaaaacaca ccaccacga 
      gtaaccaaa atcagcgtc aatgggcaa gttgtatgt 
      tcaggactg cccatagaa atgaacaat tgggttcgt 
      gtcttaacc aaaatcaac ctcgaacac accctgacc 
      atcaaagtg tcacctctt cgatcacag agcagccct 
      tttgtacca tcacgaaat aacccattc ctgtcggaa 
      aaggcaaat ccttttcag gacaccaaa gttgtcacg 
      aaaccaatt cgcaaccaa tttttggac gaattcccg 
      gctccagtt aggaccaca acagagaag attttcaga 
      agctcagga ccctccaat gaattgttt ggaggcaac 
      ccattcctc aatggtaag cccacagta ccaccaacc 
      cagccccca ccaccaggc acgtgtggc gtgtcaatc 
      gtagcaaac ccattggta atcaatggt aacaccgtt 
      ccacgtggt gccttccca tggcttact gcgattttt 
      gctgttacc accaccggt cttgagtac aaatgtagc 
      ggttccctg gtctcccaa aagcacata attaccgcc 
      gcccattgc gtgcaagaa gggcgcaaa aggccccaa 
      cccgagcgc ttcctcttc gtcctcggc aaactgaac 
      atcaagaaa tggtcgctc agcgagggc gaaaaaatg 
      gtcgaagcc gaggacatc cgaatacac ccggactat 
      gtccctttg acgtcggac gcggacata gccgtcgtg 
      attctggcc gagaagatc gacttcagc aagtacatc 
      cggcccata tgtctgtgg tccgagccg gacgatgtg 
      gacaaaatc gtgggccaa aagggcaag gtagtgggc 
      tggggccgc gacgagcag gacaacttg atgacggct 
      gagcccaaa caggccgat attcccgtc gtgggccag 
      gaggagtgt ctgcgctcg tcggaggcc ttcaggtac 
      atcacgtcg gagaggacc ttctgtgcc ggggagagg 
      aaccaaagt gggccttgg cgtgtgcac agtgtttct 
      cctgtggtg ccttcactt ccactggaa aagatggcg 
      gcttttccg gatgtcctg gcacgtgcc aggaccaaa 
      aaatgtgcc cctgctggg cgcaagtta cctgttttt 
      gggcaaagc gcgccagaa ttgccgaac gtaaaaatt 
      tggaagaaa cacagacga attatagtg ccgacgcta 
      aaccagtcg attttgaac actttcccg caaattatt 
      tgccaacag tgcgacatt ttgttggaa atactcgaa 
      aaaaagtgc gacaaaggc gaaatagat cactacaag 
      tttgtgaca aactttgca gtggatgtt gttagtgaa 
      accattttt ggggttccc ctgaacgcg caaatcacg 
      gacgccaat tacagtcgc acctttgac aagataatg 
      gaagtggtt tttatgcga attttccgc gtggattat 
      cactcggat tttctcttc tcctggacg aaagaatac 
      aaggcggag caggaaaat gtgaagctg gttaaggag 
      acaacgcgt gatttgatc gagcggaaa aaggagcaa 
      attacgtgt caattagaa gtcgaagag gagaaaaaa 
      cggccgttt cttgacgtc cttgttggg aaatatttg 
      aatgaggaa ctgagttat caggaattg gaggatgaa 
      gttagcacg tttctcttg gctggttct gacacgaat 
      gcgacggcg ggttgtttc gttttgacg cttttaggg 
      atgcatcaa gacgttcag gaaaaactc tacgaagaa 
      ataatcgaa gttctgggc cccgaaaaa tacccaacc 
      cttgacgat ttaccaaaa ctaaaatac acagaacgt 
      gtgattaaa gaaacgctt cggttattc cccggagct 
      ccgttcatt gcaagaata gcatcagac gacattgat 
      ttaggtgat tatgtgatt ccccgaggt tcaaatata 
      gccgtaggt tacgtccat ttgcacaga agtgaaaaa 
      tactgggaa gagccttta aaatttaat cctgagcgc 
      tttttgccc gaaaacgtg gcaaagaga cacccgtac 
      acgtggttg cctttctca ggcgggcta aggaattgt 
      gtcggcgga aaatttgga atgatggtc atgaaaata 
      atgataagc atgattata cgaaaattt agggtgaaa 
      agttcggtc aagagtgtt ggagatatt gaattgacg 
      gcaaatatt gttttgaag cccaaaaat gggttcaga 
      ttggccttt accttgcgt taa
    mRNA sequence
    • >TC015295 RA
      ttctttttt attacgagc tgcgatggc tcgaaagcg 
      tgccagttt tcggtgagg tagagctta aagttggtt 
      ttagtcggc tggttaaat aaagttgag gctagaaga 
      gacagtttt tgggtgatt ggtttgctc aatcgccgg 
      aaccgaatg tcatcagtg tcaccactc aagcatgcc 
      cgtgtaaaa gatgcacat cttgagctt ccttctttg 
      cgtcgtcac aacgaaagc catggtcgt cagacccca 
      gctgtactc cagcccatg cccagacac cttccagta 
      cgaaatgga ccagaatgg gcagctgta tggtactat 
      tggagtcta ctccttcga cgagaacat cgtcagact 
      caacgtcga gctgtcggt tggaaaccg cgtgaacag 
      ttacaatgg cgagatcca gttagccaa tcccaaaga 
      actcatctt cgacgacat ccagcaaca aagacccat 
      caaatataa agttttctt ccctcgatg ggaaaacac 
      accaccacg agtaaccaa aatcagcgt caatgggca 
      agttgtatg ttcaggact gcccataga aatgaacaa 
      ttgggttcg tgtcttaac caaaatcaa cctcgaaca 
      caccctgac catcaaagt gtcacctct tcgatcaca 
      gagcagccc ttttgtacc atcacgaaa taacccatt 
      cctgtcgga aaaggcaaa tccttttca ggacaccaa 
      agttgtcac gaaaccaat tcgcaacca atttttgga 
      cgaattccc ggctccagt taggaccac aacagagaa 
      gattttcag aagctcagg accctccaa tgaattgtt 
      tggaggcaa cccattcct caatggtaa gcccacagt 
      accaccaac ccagccccc accaccagg cacgtgtgg 
      cgtgtcaat cgtagcaaa cccattggt aatcaatgg 
      taacaccgt tccacgtgg tgccttccc atggcttac 
      tgcgatttt tgctgttac caccaccgg tcttgagta 
      caaatgtag cggttccct ggtctccca aaagcacat 
      aattaccgc cgcccattg cgtgcaaga agggcgcaa 
      aaggcccca acccgagcg cttcctctt cgtcctcgg 
      caaactgaa catcaagaa atggtcgct cagcgaggg 
      cgaaaaaat ggtcgaagc cgaggacat ccgaataca 
      cccggacta tgtcccttt gacgtcgga cgcggacat 
      agccgtcgt gattctggc cgagaagat cgacttcag 
      caagtacat ccggcccat atgtctgtg gtccgagcc 
      ggacgatgt ggacaaaat cgtgggcca aaagggcaa 
      ggtagtggg ctggggccg cgacgagca ggacaactt 
      gatgacggc tgagcccaa acaggccga tattcccgt 
      cgtgggcca ggaggagtg tctgcgctc gtcggaggc 
      cttcaggta catcacgtc ggagaggac cttctgtgc 
      cggggagag gaaccaaag tgggccttg gcgtgtgca 
      cagtgtttc tcctgtggt gccttcact tccactgga 
      aaagatggc ggcttttcc ggatgtcct ggcacgtgc 
      caggaccaa aaaatgtgc ccctgctgg gcgcaagtt 
      acctgtttt tgggcaaag cgcgccaga attgccgaa 
      cgtaaaaat ttggaagaa acacagacg aattatagt 
      gccgacgct aaaccagtc gattttgaa cactttccc 
      gcaaattat ttgccaaca gtgcgacat tttgttgga 
      aatactcga aaaaaagtg cgacaaagg cgaaataga 
      tcactacaa gtttgtgac aaactttgc agtggatgt 
      tgttagtga aaccatttt tggggttcc cctgaacgc 
      gcaaatcac ggacgccaa ttacagtcg cacctttga 
      caagataat ggaagtggt ttttatgcg aattttccg 
      cgtggatta tcactcgga ttttctctt ctcctggac 
      gaaagaata caaggcgga gcaggaaaa tgtgaagct 
      ggttaagga gacaacgcg tgatttgat cgagcggaa 
      aaaggagca aattacgtg tcaattaga agtcgaaga 
      ggagaaaaa acggccgtt tcttgacgt ccttgttgg 
      gaaatattt gaatgagga actgagtta tcaggaatt 
      ggaggatga agttagcac gtttctctt ggctggttc 
      tgacacgaa tgcgacggc gggttgttt cgttttgac 
      gcttttagg gatgcatca agacgttca ggaaaaact 
      ctacgaaga aataatcga agttctggg ccccgaaaa 
      atacccaac ccttgacga tttaccaaa actaaaata 
      cacagaacg tgtgattaa agaaacgct tcggttatt 
      ccccggagc tccgttcat tgcaagaat agcatcaga 
      cgacattga tttaggtga ttatgtgat tccccgagg 
      ttcaaatat agccgtagg ttacgtcca tttgcacag 
      aagtgaaaa atactggga agagccttt aaaatttaa 
      tcctgagcg ctttttgcc cgaaaacgt ggcaaagag 
      acacccgta cacgtggtt gcctttctc aggcgggct 
      aaggaattg tgtcggcgg aaaatttgg aatgatggt 
      catgaaaat aatgataag catgattat acgaaaatt 
      tagggtgaa aagttcggt caagagtgt tggagatat 
      tgaattgac ggcaaatat tgttttgaa gcccaaaaa 
      tgggttcag attggcctt taccttgcg ttaagcaat 
      tcagtacaa ttgtcttat ttaaaatat ataataaaa 
      ttctttttt tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY