Status
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Gene information
General information
TC033335
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015286,TC015287,TC015289,TC015288,TC015290
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 918714..935865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033335
    iBeetle screen iB_05790

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033335 RA
      MQPDIDVTS ALTWAISAL ALFFLVKYN WKRRWLYYH 
      GSKIDGPFG WPIIGSAHY FIGGQKGTK KTQHDSIKP 
      SFLVFYQKM VKVFETQPS VFKFWLGKD LLVVTSKPE 
      DVEIVLNNN SCFGKPKFY DFAFKLFRN GLLIAPASI 
      WRDRRRVIN PTFNPKILN SFIEIFGKH GNKLVKVLE 
      ESCSESDIF LKLFRCTFD IACETLADV DSELIKGQD 
      KYLQTVIRM EDILAIRSF SIWLHPDFL WKRSFYGKE 
      MEKASAEVF AFVKQILAL KKASVTDDE NYKKKRFLN 
      HLLTTSETN PQIDELAIL EEIQTILVT GSETTAITI 
      GMVFIILGI YPEIQEKVR SELELILGP DDREITLED 
      INNLEYLER VIKETLRVL PIVPLITRT VEQDVKLGT 
      KTIPSGSFV LVPIASIGK KAEFWAEPK KFDPDRFLP 
      ENNANRPRC SFIPFSYGP RNCIGFKYG MMSLKVLLA 
      TVIRKFTFK PSQYRRIED VRLIYGMVA KPKHALWSV 
      CNHSFTMKR DYIDITCAL TWAFSALAL VFIIKYNWS 
      RRWLYYYGS KISGPFAWP IIGSAHHFI GGQKVFYKN 
      MIKLFETQP PIFKFWLGK DLIVVTSRP EDVETVLSN 
      CFGKPKFYD YSYKLFRDG LLIAPAKIW KDRRKIINP 
      TFNPRILNS FLDIFNKHA NRVVDVFAE GCGKESFDV 
      FLKLFRCTL DIACETLAD VDSDLIKGQ DAYLQKAIR 
      MEDVLAIRS FSVWLHPDI VWNNSSFGK EVAKASPEV 
      FGFIKQIIG LKKLNPVPE VSEDFTIED EFCKKKRFV 
      NHLLTASET NPHFDELAV EEETQTILI TGSETTAIT 
      IGMVLIILG IYPEIQEKI MDELDLVLG PDDRTITLE 
      DINKMEYLE RVIKETLRV LPIVPIILR SVDEDIKLD 
      PYTIPAGSF VLVPIGHIG KKPEFWKEA NKFDPDRFL 
      PENNSNRHR CTFIPFSYG ARNCVGFKY GMMSMKVLL 
      AAILRKYNV KPAQYKIFY KTLVHFVET QPEIFKFWL 
      GQHLLVVTS RPEDVDCVV NKFFEKGPF FEFGKQLLG 
      DGLLRAPAH IWKDRRKMF NPTFRTKIL NTFIGTFGR 
      HANKLVVEL EECCGKQSC DLLFKLLRC SLDTACETL 
      TDVDSTLLE GKDNYLRNM IRVEDIVSV RTLSFWYHV 
      DIFWKMSPL SREMDKGCE ELFSFIKQV IELKKLNRS 
      QNNRDKDNA IKEKLFINH LLNLSETDR KIDDRVVEE 
      EIQNILLTS TETSTLGVG MVLIILGIL PKIQGKVSE 
      ELKTIFGTD DRQPTLEDI NKLEYLERV IKETFRLFP 
      VVPMFIRSA DHDIKFDCY TIPAGSIIL IPIFQLNKK 
      PEFWNEPQK FDPDRFLPE NNSNRHRCT FIPFSYGPR 
      NCLGLKYGM MSMKVVLST VLRNYTIKP TVYKKLDDI 
      EMIFGIVNK PSLGFKVKL EKKLSLAAI NETKGQQFP 
      HTQAKNSVK MICENVLVW LFSTLIAFL LLNAWHKRR 
      LLYYASKIN GPFSWPIIG SAHYFIGGQ KVFYKKITQ 
      LLETQPEIF KIWLGGQLV VVTSRPEDV EVIVNKFFE 
      KGPIVEYSK KFLGNSLLR APAHIWKDR RKMLNPTFN 
      QKILNTFMG TFATHAHKL VKELEEHCG KDYDVFSNL 
      IRCTLNLAI ENLADVDSD LIQGQDNYL QNVKRLEDL 
      LAIRIFTFW FHADFFWKM SPLSREMHK ATEETFAFV 
      KQIIKTKKM NVKQDNNSP TKGKLFINH LIKVSKTDA 
      KIDDLAIEE EIQNILIAS SETTALTAG LVLTILGIF 
      PEIQFKVSN ELGAVFGHD GRAPSLEDI NKMEYLECV 
      IKETLRLFP VLPIILRFL DQDIKLGAY TIPAGCSIA 
      IPICHLNKK ADFWENPEK FDPDRFLRM NSSERHRCT 
      FIPFSYGPR NCIGLKYGM MSLKVLLST ILRNYTIKP 
      SVYEKLEDI EMVFCVLSK PSLGFKVKL EKKIRNRPE 
      MFDFIFVSL TLILAFIIK YNWDRRWLY YYGSKLDSP 
      IGWPIIGSA HYIIGGHKV FYKNVNMLL KSYPSEVAK 
      IWIGPTLVV SITKPEYVE IVLNKCLER PEFYEFGKQ 
      IIGAGILTA PIDVWKSRR KMINPTFNP NILNSFVEI 
      FGRYAFHLT NALEENCGK ESFDILPKL FKCTFETAC 
      ETLGDVNSN VLQGPEEIF ENLTKAEDL ITTRAFSLW 
      LYLDFFWNM TSFCRELDK ASKSIISIA KQVIQIKKS 
      SRNQKPSEP MIQDNTYKE KRFVNHLLK LSETNAKLD 
      QTALADEIQ TFLLAGSET TALTVGLTL IILGIYPEI 
      QKKIGKELE VIFGKDARV PTLEDINRM EYLERVIKE 
      TLRFLTPVP FMLRTNNQD ITLDSNTIP AGSCIMIPI 
      FHIHKKPEY WKNPNEFDP DRFLPENSS KRPRCAFIP 
      FSSGPRNCI GFKYGMMSV KVLLAVILR KYTVVATEY 
      KKVEDIEML FYLVNKPIS GCKIKLEKK
    CDS sequence
    • >TC033335 RA
      atgcagcct gatattgac gttacatcc gccctaaca 
      tgggccatc tcagccctt gcccttttt ttcctagta 
      aaatacaac tggaaaaga cgctggctg tactatcac 
      ggctccaaa atcgacggc ccttttggc tggcccatc 
      attggaagc gcccattac ttcatcggg ggccaaaaa 
      ggtacaaaa aaaacccaa cacgattca atcaaacct 
      tcatttcta gtattttac caaaaaatg gtcaaagtg 
      tttgaaacc caaccgtcc gttttcaaa ttctggtta 
      ggtaaggat ttgttagtg gtgacctca aaaccggaa 
      gatgttgaa atcgtgtta aacaacaac agttgcttc 
      ggaaaaccc aagttttac gattttgcc tttaaactg 
      ttcaggaat gggctctta atagcgcca gcgagcatt 
      tggagggat cgcagacgt gtgattaac ccgactttt 
      aatcccaaa attttgaat tcgttcatc gagattttc 
      ggcaaacat gggaataaa ttagtgaaa gttcttgaa 
      gaaagttgc agtgagagt gatattttt ttgaaatta 
      tttaggtgc acttttgat atcgcctgt gagacactg 
      gctgatgtc gacagcgag ctaatcaaa gggcaggat 
      aagtacctt cagaccgta ataagaatg gaagatatt 
      ttggcaatt cgttcgttc agtatttgg cttcaccct 
      gacttcctc tggaagcgt tctttctat ggaaaagag 
      atggaaaaa gcgtctgct gaagtgttc gctttcgtg 
      aaacaaatt ttggccttg aagaaagca tcggttact 
      gacgatgaa aattacaag aagaaacgt ttcctgaac 
      catttatta acaacaagt gagacaaac ccacaaata 
      gacgaactc gcaattttg gaagaaatc caaacaatc 
      ctggtgaca ggaagcgaa accactgct atcacaatt 
      gggatggtt ttcattatt ttagggata tatccagaa 
      attcaggaa aaagtgaga tcggaactc gagttaatt 
      ttgggcccc gatgaccgg gaaataact ctggaagac 
      ataaataac ctggaatat ttagaacgt gtgattaag 
      gaaaccttg agagtgctc ccaatcgtc ccactgatc 
      accagaacg gtggaacag gacgtgaaa ctgggcact 
      aaaaccatc ccatctggt agttttgtc ctggttccc 
      attgcttcc attggtaaa aaggccgaa ttttgggcg 
      gagccgaaa aaatttgac cctgatcgg tttttgccc 
      gaaaataat gccaatcgg ccacggtgt tcttttatt 
      ccgtttagt tacggtccc agaaattgc atcggtttc 
      aaatacggt atgatgtct ctgaaggtg cttttggcc 
      acagttata agaaaattc accttcaag ccgtcacag 
      tataggaga attgaagac gtccggttg atttatggc 
      atggttgcg aaaccgaaa catgcactt tggtccgtt 
      tgcaatcac tcgtttaca atgaaacgc gactacatt 
      gacatcacc tgtgcccta acttgggcc ttctcggcc 
      cttgcccta gttttcata atcaagtac aattggagt 
      aggcgctgg ttgtactac tatggctcg aaaataagc 
      ggacccttc gcctggccc atcattggc agcgcccat 
      cacttcatc ggaggccag aaagtgttt tacaaaaac 
      atgatcaag ttgtttgag acccaaccg ccaattttc 
      aaattctgg ctcggtaag gatttgatt gttgtgacg 
      tcaaggccg gaagatgtt gagactgtt ttgagtaat 
      tgtttcgga aaaccgaaa ttctacgat tattcgtat 
      aaactgttc agggatgga cttttgata gcgccagca 
      aaaatttgg aaagatcgc aggaaaatt attaatccg 
      acctttaat cccagaatt ttgaactcg ttcctggac 
      atttttaat aaacatgcc aatcgggta gttgatgtt 
      tttgcggaa ggttgcggt aaagaatcg tttgatgtt 
      ttcttgaaa ctgttcaga tgcactttg gatattgcc 
      tgtgagaca cttgctgat gttgacagt gatttaatc 
      aaaggtcag gacgcctat ttacaaaaa gcaataagg 
      atggaggat gtcttagca attcgttcg ttcagcgtt 
      tggctgcat ccagacatc gtttggaac aattcctcg 
      tttgggaaa gaagtagca aaagcatct ccagaagtg 
      ttcggtttt attaaacaa atcataggc ttgaagaaa 
      ttaaaccca gtcccggaa gttagtgaa gattttacg 
      atagaggac gaattttgt aagaaaaaa cgtttcgtt 
      aaccatttg ttgactgcc agtgagaca aacccacat 
      tttgacgaa ttggctgtt gaggaggaa acccaaact 
      attcttatc actggtagc gaaactaca gctatcaca 
      attgggatg gttttgatt attttgggc atataccca 
      gaaattcag gaaaaaatt atggacgaa ctcgaccta 
      gttttggga cccgatgac agaacaata actctagaa 
      gacataaac aaaatggaa tatctggaa cgtgtgatt 
      aaagaaacc ctgagagta cttccaatc gtaccaata 
      attttacgt tcagtggac gaagacata aaattagat 
      ccttacaca atcccggct ggtagtttc gtcttagtg 
      ccaatcggt cacattggc aaaaaaccc gaattttgg 
      aaagaagca aacaaattc gatcctgat cgatttttg 
      cccgaaaat aactcgaat cgacatcgt tgtacgttc 
      attcctttt agttacggg gcccgaaat tgtgtgggt 
      tttaaatac ggtatgatg tccatgaag gtgctttta 
      gcagcaatt ttgaggaaa tacaacgtc aaacctgca 
      cagtataaa atattttac aaaacattg gtccatttt 
      gtggaaacc caaccggaa atatttaaa ttctggttg 
      ggccaacat ttactagtc gtgacgtca cgaccggaa 
      gacgtcgat tgtgtcgta aataaattt ttcgaaaaa 
      gggccattt tttgaattt ggtaaacaa ttgctcgga 
      gatggttta ctaagagct ccagctcac atttggaaa 
      gatcgccga aaaatgttc aatccaaca tttaggacg 
      aaaattttg aacacattt attggcact tttggacga 
      catgccaat aaactggta gtggagctt gaagagtgc 
      tgtggaaaa caatcgtgt gacctactt ttcaaactg 
      ttgaggtgt agtttagat acagcgtgt gaaacttta 
      acagacgtt gacagcact ttactggag ggaaaagac 
      aattattta cgaaatatg attagagtt gaggatatt 
      gtgtcagtc cgtacttta agtttttgg taccatgtt 
      gatatcttt tggaaaatg tcacctcta agcagagaa 
      atggataaa ggatgtgag gaattattt tcgttcata 
      aaacaagtt atagaatta aaaaaatta aaccgaagc 
      cagaacaat agagataaa gataatgcc atcaaagaa 
      aaactcttt ataaatcat ttactaaac ttgagtgaa 
      actgataga aaaattgac gatagggtt gtagaagag 
      gaaattcaa aatatttta cttacgagt accgaaact 
      tcaactctg ggagttgga atggtttta ataattttg 
      ggaatattg ccaaaaata cagggtaaa gtgagtgag 
      gaactgaaa actattttt ggtacggat gacagacag 
      ccaactttg gaagatatt aacaagttg gagtaccta 
      gaacgtgtc attaaggag acttttagg ttatttccg 
      gtagtacca atgttcata agatcagct gaccatgac 
      ataaaattt gattgttat acaatccca gctgggagt 
      attatcctg attccaatt tttcaactt aacaaaaaa 
      cccgaattt tggaacgaa ccacaaaaa ttcgaccct 
      gatcgattt ttaccagag aataactcc aatcggcac 
      cgttgtaca tttattccg tttagttat gggcccaga 
      aattgtctc ggtctgaag tacggtatg atgtcgatg 
      aaagtcgta ctatcgact gttttacga aattatacg 
      attaaacca actgtttat aaaaagttg gacgatatc 
      gagatgatt tttgggatc gtgaataaa cctagtttg 
      gggttcaaa gtcaagttg gagaaaaaa ttgtcatta 
      gcagctata aatgaaaca aaaggacaa caattccca 
      catactcaa gccaaaaat agcgtaaaa atgatttgt 
      gaaaatgtc ctcgtttgg ctattttcc accttaatt 
      gcgttcttg ttgctaaac gcctggcat aagcgacgt 
      cttctttat tacgcctcc aaaataaac ggccctttt 
      tcctggccc atcattgga agcgcccat tacttcatc 
      gggggccaa aaagtattt tacaaaaaa ataacccaa 
      ctgttggaa acacaaccg gaaatattc aaaatctgg 
      ttgggtgga caactagtt gtggtaact tcaaggccc 
      gaagatgtt gaagttatt gtgaacaag ttttttgaa 
      aagggacca attgttgaa tattcgaaa aaattcctc 
      ggaaatagt cttctaaga gccccagct catatctgg 
      aaggatcga agaaaaatg ctcaaccca acatttaac 
      caaaaaatt ttaaacact tttatgggc acttttgca 
      acacatgcc cataagttg gtcaaagaa cttgaagaa 
      cattgtggg aaagactat gatgttttt tcaaacttg 
      ataaggtgc actttaaat ttagcaata gagaatttg 
      gcggacgtg gacagtgat ttaatccaa ggacaagac 
      aattatttg caaaacgta aagagatta gaggacctt 
      ttggcgatt cgcattttc acattttgg tttcacgct 
      gattttttt tggaaaatg tccccattg agtagagaa 
      atgcataaa gcaactgag gaaacgttt gctttcgtt 
      aagcaaatc atcaaaacg aaaaaaatg aacgtgaaa 
      caagacaat aacagtccg accaaagga aaacttttc 
      ataaatcac ctcataaaa gttagcaaa accgacgca 
      aaaatcgac gatttggca atcgaggag gaaatccaa 
      aacattctg attgctagc agcgaaact acagctctt 
      acagctggc ctggttttg acaattttg ggaatattt 
      ccagaaata cagtttaaa gtttcgaac gaacttggg 
      gcggttttt gggcatgat ggaagagcg ccatctctg 
      gaagacatt aacaagatg gagtacttg gaatgcgtt 
      attaaggaa actctgaga ttatttcca gtactgccg 
      ataattttg cgattcctg gaccaggac ataaaattg 
      ggcgcttat acgatccca gctggctgt agcatcgcc 
      attccaatt tgtcacctc aataaaaag gcagatttt 
      tgggaaaat ccggaaaaa tttgatcca gatcgcttt 
      ttgcgaatg aatagttcc gagcgtcat cgttgtacg 
      tttattcct tttagttat gggcccaga aattgcata 
      ggcttgaag tacggtatg atgtcattg aaggttttg 
      ttatcgacg attttacgc aattatacg attaaacca 
      tcggtttat gaaaagttg gaagatatc gagatggtt 
      ttttgtgtg ctgagcaaa ccaagtttg gggttcaaa 
      gtcaaactg gagaaaaaa attagaaat cggcccgaa 
      atgttcgac ttcatattt gtttccttg actttgatt 
      ctagctttc attataaag tataactgg gacaggcga 
      tggctttac tattatggt tcaaaatta gatagtccc 
      attggttgg cccatcatt ggcagtgcc cattatatc 
      atcggcggt cataaagtt ttctacaaa aacgtaaat 
      atgctccta aaatcatat ccttcagaa gttgccaaa 
      atatggata ggtcccact ttagtagta tcgattaca 
      aaaccagaa tatgttgaa attgtgcta aataaatgt 
      ttggaaaga ccggagttt tacgagttc ggtaaacag 
      ataatcgga gctgggatt ctaacagcg ccaattgat 
      gtttggaaa agcagacgg aaaatgatt aatccaact 
      tttaaccca aacattttg aactctttt gttgaaatt 
      ttcggacga tatgcgttt catttaaca aatgcactt 
      gaggaaaac tgcggaaaa gagtctttt gatatttta 
      ccaaaattg tttaaatgc acttttgag accgcttgt 
      gaaacgctt ggtgacgtt aacagtaat gtacttcaa 
      ggcccggaa gagattttc gaaaattta acaaaggca 
      gaagatctt ataacgact cgtgcattt tccctttgg 
      ctgtatctt gatttcttc tggaacatg acttcgttt 
      tgtagggaa ttggataaa gcgagtaaa tcaataatt 
      tcaattgca aaacaagta atacaaata aaaaaatca 
      agtcgtaac caaaaacca agtgaacca atgatacaa 
      gacaatact tacaaagaa aaacgtttt gtaaatcat 
      ttgttaaaa ttgagcgaa acaaatgcg aaactcgac 
      caaacagcc ttagcagac gagatccaa acattttta 
      cttgctggg agcgaaact acagctctt acagttggt 
      ttaacttta attatttta ggaatatac ccagaaata 
      cagaaaaaa attggtaaa gaacttgag gtcatcttc 
      ggcaaagat gccagggtg ccaacttta gaagacatt 
      aacagaatg gagtacctg gaaagggtc attaaggag 
      actttgaga ttcctaaca cccgtacca ttcatgtta 
      cgaactaat aaccaagat ataacgctt gattcgaac 
      acaatcccg gctggaagt tgcataatg atcccaatt 
      tttcacatt cacaagaaa ccggaatat tggaaaaat 
      ccgaatgaa ttcgatcct gatcgcttt ttgcctgaa 
      aatagttcg aagcggcct cggtgtgct tttattccg 
      tttagttca gggcctaga aattgcata ggttttaaa 
      tacggcatg atgtcagtt aaagttcta ttggctgtg 
      attttacga aagtacaca gttgtggca actgaatat 
      aaaaaagtg gaagacatc gaaatgctc ttctatctg 
      gtgaacaaa ccaatctca ggatgtaag atcaagttg 
      gagaaaaaa taa
    mRNA sequence
    • >TC033335 RA
      gtacaacat gcagcctga tattgacgt tacatccgc 
      cctaacatg ggccatctc agcccttgc ccttttttt 
      cctagtaaa atacaactg gaaaagacg ctggctgta 
      ctatcacgg ctccaaaat cgacggccc ttttggctg 
      gcccatcat tggaagcgc ccattactt catcggggg 
      ccaaaaagg tacaaaaaa aacccaaca cgattcaat 
      caaaccttc atttctagt attttacca aaaaatggt 
      caaagtgtt tgaaaccca accgtccgt tttcaaatt 
      ctggttagg taaggattt gttagtggt gacctcaaa 
      accggaaga tgttgaaat cgtgttaaa caacaacag 
      ttgcttcgg aaaacccaa gttttacga ttttgcctt 
      taaactgtt caggaatgg gctcttaat agcgccagc 
      gagcatttg gagggatcg cagacgtgt gattaaccc 
      gacttttaa tcccaaaat tttgaattc gttcatcga 
      gattttcgg caaacatgg gaataaatt agtgaaagt 
      tcttgaaga aagttgcag tgagagtga tattttttt 
      gaaattatt taggtgcac ttttgatat cgcctgtga 
      gacactggc tgatgtcga cagcgagct aatcaaagg 
      gcaggataa gtaccttca gaccgtaat aagaatgga 
      agatatttt ggcaattcg ttcgttcag tatttggct 
      tcaccctga cttcctctg gaagcgttc tttctatgg 
      aaaagagat ggaaaaagc gtctgctga agtgttcgc 
      tttcgtgaa acaaatttt ggccttgaa gaaagcatc 
      ggttactga cgatgaaaa ttacaagaa gaaacgttt 
      cctgaacca tttattaac aacaagtga gacaaaccc 
      acaaataga cgaactcgc aattttgga agaaatcca 
      aacaatcct ggtgacagg aagcgaaac cactgctat 
      cacaattgg gatggtttt cattatttt agggatata 
      tccagaaat tcaggaaaa agtgagatc ggaactcga 
      gttaatttt gggccccga tgaccggga aataactct 
      ggaagacat aaataacct ggaatattt agaacgtgt 
      gattaagga aaccttgag agtgctccc aatcgtccc 
      actgatcac cagaacggt ggaacagga cgtgaaact 
      gggcactaa aaccatccc atctggtag ttttgtcct 
      ggttcccat tgcttccat tggtaaaaa ggccgaatt 
      ttgggcgga gccgaaaaa atttgaccc tgatcggtt 
      tttgcccga aaataatgc caatcggcc acggtgttc 
      ttttattcc gtttagtta cggtcccag aaattgcat 
      cggtttcaa atacggtat gatgtctct gaaggtgct 
      tttggccac agttataag aaaattcac cttcaagcc 
      gtcacagta taggagaat tgaagacgt ccggttgat 
      ttatggcat ggttgcgaa accgaaaca tgcactttg 
      gtccgtttg caatcactc gtttacaat gaaacgcga 
      ctacattga catcacctg tgccctaac ttgggcctt 
      ctcggccct tgccctagt tttcataat caagtacaa 
      ttggagtag gcgctggtt gtactacta tggctcgaa 
      aataagcgg acccttcgc ctggcccat cattggcag 
      cgcccatca cttcatcgg aggccagaa agtgtttta 
      caaaaacat gatcaagtt gtttgagac ccaaccgcc 
      aattttcaa attctggct cggtaagga tttgattgt 
      tgtgacgtc aaggccgga agatgttga gactgtttt 
      gagtaattg tttcggaaa accgaaatt ctacgatta 
      ttcgtataa actgttcag ggatggact tttgatagc 
      gccagcaaa aatttggaa agatcgcag gaaaattat 
      taatccgac ctttaatcc cagaatttt gaactcgtt 
      cctggacat ttttaataa acatgccaa tcgggtagt 
      tgatgtttt tgcggaagg ttgcggtaa agaatcgtt 
      tgatgtttt cttgaaact gttcagatg cactttgga 
      tattgcctg tgagacact tgctgatgt tgacagtga 
      tttaatcaa aggtcagga cgcctattt acaaaaagc 
      aataaggat ggaggatgt cttagcaat tcgttcgtt 
      cagcgtttg gctgcatcc agacatcgt ttggaacaa 
      ttcctcgtt tgggaaaga agtagcaaa agcatctcc 
      agaagtgtt cggttttat taaacaaat cataggctt 
      gaagaaatt aaacccagt cccggaagt tagtgaaga 
      ttttacgat agaggacga attttgtaa gaaaaaacg 
      tttcgttaa ccatttgtt gactgccag tgagacaaa 
      cccacattt tgacgaatt ggctgttga ggaggaaac 
      ccaaactat tcttatcac tggtagcga aactacagc 
      tatcacaat tgggatggt tttgattat tttgggcat 
      atacccaga aattcagga aaaaattat ggacgaact 
      cgacctagt tttgggacc cgatgacag aacaataac 
      tctagaaga cataaacaa aatggaata tctggaacg 
      tgtgattaa agaaaccct gagagtact tccaatcgt 
      accaataat tttacgttc agtggacga agacataaa 
      attagatcc ttacacaat cccggctgg tagtttcgt 
      cttagtgcc aatcggtca cattggcaa aaaacccga 
      attttggaa agaagcaaa caaattcga tcctgatcg 
      atttttgcc cgaaaataa ctcgaatcg acatcgttg 
      tacgttcat tccttttag ttacggggc ccgaaattg 
      tgtgggttt taaatacgg tatgatgtc catgaaggt 
      gcttttagc agcaatttt gaggaaata caacgtcaa 
      acctgcaca gtataaaat attttacaa aacattggt 
      ccattttgt ggaaaccca accggaaat atttaaatt 
      ctggttggg ccaacattt actagtcgt gacgtcacg 
      accggaaga cgtcgattg tgtcgtaaa taaattttt 
      cgaaaaagg gccattttt tgaatttgg taaacaatt 
      gctcggaga tggtttact aagagctcc agctcacat 
      ttggaaaga tcgccgaaa aatgttcaa tccaacatt 
      taggacgaa aattttgaa cacatttat tggcacttt 
      tggacgaca tgccaataa actggtagt ggagcttga 
      agagtgctg tggaaaaca atcgtgtga cctactttt 
      caaactgtt gaggtgtag tttagatac agcgtgtga 
      aactttaac agacgttga cagcacttt actggaggg 
      aaaagacaa ttatttacg aaatatgat tagagttga 
      ggatattgt gtcagtccg tactttaag tttttggta 
      ccatgttga tatcttttg gaaaatgtc acctctaag 
      cagagaaat ggataaagg atgtgagga attattttc 
      gttcataaa acaagttat agaattaaa aaaattaaa 
      ccgaagcca gaacaatag agataaaga taatgccat 
      caaagaaaa actctttat aaatcattt actaaactt 
      gagtgaaac tgatagaaa aattgacga tagggttgt 
      agaagagga aattcaaaa tattttact tacgagtac 
      cgaaacttc aactctggg agttggaat ggttttaat 
      aattttggg aatattgcc aaaaataca gggtaaagt 
      gagtgagga actgaaaac tatttttgg tacggatga 
      cagacagcc aactttgga agatattaa caagttgga 
      gtacctaga acgtgtcat taaggagac ttttaggtt 
      atttccggt agtaccaat gttcataag atcagctga 
      ccatgacat aaaatttga ttgttatac aatcccagc 
      tgggagtat tatcctgat tccaatttt tcaacttaa 
      caaaaaacc cgaattttg gaacgaacc acaaaaatt 
      cgaccctga tcgattttt accagagaa taactccaa 
      tcggcaccg ttgtacatt tattccgtt tagttatgg 
      gcccagaaa ttgtctcgg tctgaagta cggtatgat 
      gtcgatgaa agtcgtact atcgactgt tttacgaaa 
      ttatacgat taaaccaac tgtttataa aaagttgga 
      cgatatcga gatgatttt tgggatcgt gaataaacc 
      tagtttggg gttcaaagt caagttgga gaaaaaatt 
      gtcattagc agctataaa tgaaacaaa aggacaaca 
      attcccaca tactcaagc caaaaatag cgtaaaaat 
      gatttgtga aaatgtcct cgtttggct attttccac 
      cttaattgc gttcttgtt gctaaacgc ctggcataa 
      gcgacgtct tctttatta cgcctccaa aataaacgg 
      ccctttttc ctggcccat cattggaag cgcccatta 
      cttcatcgg gggccaaaa agtatttta caaaaaaat 
      aacccaact gttggaaac acaaccgga aatattcaa 
      aatctggtt gggtggaca actagttgt ggtaacttc 
      aaggcccga agatgttga agttattgt gaacaagtt 
      ttttgaaaa gggaccaat tgttgaata ttcgaaaaa 
      attcctcgg aaatagtct tctaagagc cccagctca 
      tatctggaa ggatcgaag aaaaatgct caacccaac 
      atttaacca aaaaatttt aaacacttt tatgggcac 
      ttttgcaac acatgccca taagttggt caaagaact 
      tgaagaaca ttgtgggaa agactatga tgttttttc 
      aaacttgat aaggtgcac tttaaattt agcaataga 
      gaatttggc ggacgtgga cagtgattt aatccaagg 
      acaagacaa ttatttgca aaacgtaaa gagattaga 
      ggacctttt ggcgattcg cattttcac attttggtt 
      tcacgctga ttttttttg gaaaatgtc cccattgag 
      tagagaaat gcataaagc aactgagga aacgtttgc 
      tttcgttaa gcaaatcat caaaacgaa aaaaatgaa 
      cgtgaaaca agacaataa cagtccgac caaaggaaa 
      acttttcat aaatcacct cataaaagt tagcaaaac 
      cgacgcaaa aatcgacga tttggcaat cgaggagga 
      aatccaaaa cattctgat tgctagcag cgaaactac 
      agctcttac agctggcct ggttttgac aattttggg 
      aatatttcc agaaataca gtttaaagt ttcgaacga 
      acttggggc ggtttttgg gcatgatgg aagagcgcc 
      atctctgga agacattaa caagatgga gtacttgga 
      atgcgttat taaggaaac tctgagatt atttccagt 
      actgccgat aattttgcg attcctgga ccaggacat 
      aaaattggg cgcttatac gatcccagc tggctgtag 
      catcgccat tccaatttg tcacctcaa taaaaaggc 
      agatttttg ggaaaatcc ggaaaaatt tgatccaga 
      tcgcttttt gcgaatgaa tagttccga gcgtcatcg 
      ttgtacgtt tattccttt tagttatgg gcccagaaa 
      ttgcatagg cttgaagta cggtatgat gtcattgaa 
      ggttttgtt atcgacgat tttacgcaa ttatacgat 
      taaaccatc ggtttatga aaagttgga agatatcga 
      gatggtttt ttgtgtgct gagcaaacc aagtttggg 
      gttcaaagt caaactgga gaaaaaaat tagaaatcg 
      gcccgaaat gttcgactt catatttgt ttccttgac 
      tttgattct agctttcat tataaagta taactggga 
      caggcgatg gctttacta ttatggttc aaaattaga 
      tagtcccat tggttggcc catcattgg cagtgccca 
      ttatatcat cggcggtca taaagtttt ctacaaaaa 
      cgtaaatat gctcctaaa atcatatcc ttcagaagt 
      tgccaaaat atggatagg tcccacttt agtagtatc 
      gattacaaa accagaata tgttgaaat tgtgctaaa 
      taaatgttt ggaaagacc ggagtttta cgagttcgg 
      taaacagat aatcggagc tgggattct aacagcgcc 
      aattgatgt ttggaaaag cagacggaa aatgattaa 
      tccaacttt taacccaaa cattttgaa ctcttttgt 
      tgaaatttt cggacgata tgcgtttca tttaacaaa 
      tgcacttga ggaaaactg cggaaaaga gtcttttga 
      tattttacc aaaattgtt taaatgcac ttttgagac 
      cgcttgtga aacgcttgg tgacgttaa cagtaatgt 
      acttcaagg cccggaaga gattttcga aaatttaac 
      aaaggcaga agatcttat aacgactcg tgcattttc 
      cctttggct gtatcttga tttcttctg gaacatgac 
      ttcgttttg tagggaatt ggataaagc gagtaaatc 
      aataatttc aattgcaaa acaagtaat acaaataaa 
      aaaatcaag tcgtaacca aaaaccaag tgaaccaat 
      gatacaaga caatactta caaagaaaa acgttttgt 
      aaatcattt gttaaaatt gagcgaaac aaatgcgaa 
      actcgacca aacagcctt agcagacga gatccaaac 
      atttttact tgctgggag cgaaactac agctcttac 
      agttggttt aactttaat tattttagg aatataccc 
      agaaataca gaaaaaaat tggtaaaga acttgaggt 
      catcttcgg caaagatgc cagggtgcc aactttaga 
      agacattaa cagaatgga gtacctgga aagggtcat 
      taaggagac tttgagatt cctaacacc cgtaccatt 
      catgttacg aactaataa ccaagatat aacgcttga 
      ttcgaacac aatcccggc tggaagttg cataatgat 
      cccaatttt tcacattca caagaaacc ggaatattg 
      gaaaaatcc gaatgaatt cgatcctga tcgcttttt 
      gcctgaaaa tagttcgaa gcggcctcg gtgtgcttt 
      tattccgtt tagttcagg gcctagaaa ttgcatagg 
      ttttaaata cggcatgat gtcagttaa agttctatt 
      ggctgtgat tttacgaaa gtacacagt tgtggcaac 
      tgaatataa aaaagtgga agacatcga aatgctctt 
      ctatctggt gaacaaacc aatctcagg atgtaagat 
      caagttgga gaaaaaata attggggag tggaaaaag 
      ccttgaaat tgtgtaatt tcagaaaag acaataaat 
      actttattc aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY