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Gene information
General information
TC015237 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9384835..9386685
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015237
    iBeetle screen iB_05783

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015237 RA
      MKLFFVFAL FIALVSAKP SPGLKIGRR SFLHPGARI 
      INGNDATEG QYPYQISYQ WGILGVFEH VCGGSILSP 
      TFILTAGHC VTEVPEIGA HKIVAGITE LNEKNNERQ 
      EINVVQKIV HPNFTGGVG PNDVALLKL ATPLVFGDL 
      VKPVVLPEA DSVPSGDSV LTGWGSTST TVIPVLPNH 
      LQTVTIPIL EYTDCKLAI DALLNDGEE NPLSEVSNI 
      CTHPVANGE GACSGDSGG PLAQNGTVI GIVSWGFTP 
      CGSEKAPSV YTRVSNFID FIKENVNDL PK
    CDS sequence
    • >TC015237 RA
      atgaaacta tttttcgtg tttgccctt tttatcgcc 
      cttgtttcg gctaaaccc tctccgggt ctgaaaatc 
      gggcgccgc agtttcctg caccctggt gcgcgcatc 
      atcaacggc aacgacgcc accgaaggc caatacccc 
      taccagatt tcctaccaa tggggaatt ctgggtgtt 
      ttcgagcac gtctgtgga ggttccatc ctctccccc 
      acgttcatc ctgaccgct ggacactgc gttactgaa 
      gtccctgaa atcggcgcc cacaaaatc gtagctgga 
      ataacggaa ctgaacgag aagaacaac gaaagacag 
      gaaatcaac gttgtgcaa aaaatcgtt catccaaat 
      ttcactgga ggtgtaggt ccaaacgac gttgcgctc 
      ctgaaactg gccacacct ctggtgttc ggcgatttg 
      gtgaagccc gtggttttg cctgaagcc gactctgtg 
      ccttcggga gattcggtt ctgacgggt tggggctcc 
      acttcgacc accgttatc ccagttttg cccaaccat 
      ttgcaaact gttactatt ccaatcttg gaatatacc 
      gattgcaaa ttggctatt gatgccctt ttgaatgac 
      ggtgaagaa aatcccctt tcggaagta tcgaacatt 
      tgcactcat cctgtggcc aatggcgag ggggcctgt 
      tcaggtgac agtggtggc ccattggcc cagaatggt 
      actgttatt ggaattgtt tcgtggggc ttcacacca 
      tgtgggtcg gaaaaggca ccctcggtt tacaccaga 
      gtcagcaat ttcatcgac ttcatcaag gaaaacgta 
      aacgacttg cccaaataa
    mRNA sequence
    • >TC015237 RA
      aggaaagtg tccctcaca atgaaacta tttttcgtg 
      tttgccctt tttatcgcc cttgtttcg gctaaaccc 
      tctccgggt ctgaaaatc gggcgccgc agtttcctg 
      caccctggt gcgcgcatc atcaacggc aacgacgcc 
      accgaaggc caatacccc taccagatt tcctaccaa 
      tggggaatt ctgggtgtt ttcgagcac gtctgtgga 
      ggttccatc ctctccccc acgttcatc ctgaccgct 
      ggacactgc gttactgaa gtccctgaa atcggcgcc 
      cacaaaatc gtagctgga ataacggaa ctgaacgag 
      aagaacaac gaaagacag gaaatcaac gttgtgcaa 
      aaaatcgtt catccaaat ttcactgga ggtgtaggt 
      ccaaacgac gttgcgctc ctgaaactg gccacacct 
      ctggtgttc ggcgatttg gtgaagccc gtggttttg 
      cctgaagcc gactctgtg ccttcggga gattcggtt 
      ctgacgggt tggggctcc acttcgacc accgttatc 
      ccagttttg cccaaccat ttgcaaact gttactatt 
      ccaatcttg gaatatacc gattgcaaa ttggctatt 
      gatgccctt ttgaatgac ggtgaagaa aatcccctt 
      tcggaagta tcgaacatt tgcactcat cctgtggcc 
      aatggcgag ggggcctgt tcaggtgac agtggtggc 
      ccattggcc cagaatggt actgttatt ggaattgtt 
      tcgtggggc ttcacacca tgtgggtcg gaaaaggca 
      ccctcggtt tacaccaga gtcagcaat ttcatcgac 
      ttcatcaag gaaaacgta aacgacttg cccaaataa 
      aataaatat tgtaacaac ctaacaaca atcaaataa 
      atgtgaaat aaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY