Status
Please wait ...
Gene information
General information
TC015235 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9374665..9376187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015235
    iBeetle screen iB_05782

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015235 RA
      MSFRDVRNF LEMMRALGY PSLISMESF RTPNFPLVA 
      DLLVWLAKR FDPDVDIPP EIDTEDDRV KLIRNAAQF 
      MALKANIKL NTKRLYQAD GYAVKELLK ITSLLYDAL 
      NMNLEEKED ENFEEEMLS LKDFDLSDK INELKLSRQ 
      LASEITTTG ATLFDLLGK EVELKAARH KSVSRQYEI 
      NEVEAGIKK AIEALKTEI DESKQLIDN VSATEANLD 
      NKIERRKVE IDRYEKRLQ TLKKVRPAF MEEFTALEA 
      ELEQLFVQY STRVRILNQ LERLVSETE RQQLEKQLQ 
      MVSPRIETI PLEGGTEAF LELEESEAR QKQERPRAS 
      TGGRTRTAK TAKAYGGMQ PPMSATPSL SSDSEDDEL 
      FIDKDESEL IQSDDESLG LELSGMELT KRVQSGKPA 
      GNKLNDNSD DDF
    CDS sequence
    • >TC015235 RA
      atgtcattc agagacgtg cgaaatttt cttgaaatg 
      atgcgggct ttaggatac ccgtcttta atttcaatg 
      gaaagcttt cgcacacct aattttcct ttagtcgcc 
      gatttattg gtttggtta gctaagcgg ttcgaccct 
      gatgtcgat ataccgcca gagattgat actgaagat 
      gatcgggtt aaattgatt cgaaacgcg gcgcaattt 
      atggccctg aaagccaac atcaagctg aatacaaaa 
      aggttgtat caagccgat ggttacgca gttaaagaa 
      ctgttaaaa attacgtca ctcttgtat gacgctttg 
      aatatgaat ttggaggaa aaggaggac gaaaatttt 
      gaggaagaa atgttgagt ctgaaagac tttgattta 
      agtgacaag ataaacgaa ttaaagtta tccagacaa 
      ctggcaagc gaaataact acaaccggg gccacactt 
      ttcgatctt ttgggcaaa gaagtggaa ctgaaggcg 
      gcgagacac aaaagtgtt agccgccag tacgaaatc 
      aacgaagtt gaagccgga attaagaag gcaatcgaa 
      gctcttaaa accgaaatt gacgaaagc aaacagctc 
      attgacaac gtctcagca actgaggcc aatttggac 
      aataagatc gagcgcaga aaagtcgaa atagatcgt 
      tacgaaaaa cggctccaa acgttgaaa aaagtcagg 
      ccggccttt atggaagag tttaccgct cttgaggcc 
      gaactggag caactcttt gtgcagtac tccaccaga 
      gtgcgcatt ttgaaccaa ctggaacgg ctggtgtcg 
      gagacagag cgccaacaa ttggagaaa caactccaa 
      atggtttcg cctcggatc gaaaccatt ccgctggaa 
      ggaggaact gaggcgttt ctggagttg gaggagagc 
      gaggcgagg caaaagcag gagagaccg agagctagc 
      accgggggg aggactaga actgccaag acggccaaa 
      gcttacggc ggaatgcaa ccaccgatg tccgcaacc 
      ccgtccttg agctcggat tcggaagac gacgagttg 
      tttattgac aaagacgag tcagagttg attcagtcg 
      gatgacgag tctttgggg ttggaacta tcggggatg 
      gagttgacc aaaagggtg cagtcggga aaaccggcc 
      gggaacaag ttgaatgat aacagcgat gatgatttt 
      taa
    mRNA sequence
    • >TC015235 RA
      aactgttac attcaaaca agacttaaa atgtcattc 
      agagacgtg cgaaatttt cttgaaatg atgcgggct 
      ttaggatac ccgtcttta atttcaatg gaaagcttt 
      cgcacacct aattttcct ttagtcgcc gatttattg 
      gtttggtta gctaagcgg ttcgaccct gatgtcgat 
      ataccgcca gagattgat actgaagat gatcgggtt 
      aaattgatt cgaaacgcg gcgcaattt atggccctg 
      aaagccaac atcaagctg aatacaaaa aggttgtat 
      caagccgat ggttacgca gttaaagaa ctgttaaaa 
      attacgtca ctcttgtat gacgctttg aatatgaat 
      ttggaggaa aaggaggac gaaaatttt gaggaagaa 
      atgttgagt ctgaaagac tttgattta agtgacaag 
      ataaacgaa ttaaagtta tccagacaa ctggcaagc 
      gaaataact acaaccggg gccacactt ttcgatctt 
      ttgggcaaa gaagtggaa ctgaaggcg gcgagacac 
      aaaagtgtt agccgccag tacgaaatc aacgaagtt 
      gaagccgga attaagaag gcaatcgaa gctcttaaa 
      accgaaatt gacgaaagc aaacagctc attgacaac 
      gtctcagca actgaggcc aatttggac aataagatc 
      gagcgcaga aaagtcgaa atagatcgt tacgaaaaa 
      cggctccaa acgttgaaa aaagtcagg ccggccttt 
      atggaagag tttaccgct cttgaggcc gaactggag 
      caactcttt gtgcagtac tccaccaga gtgcgcatt 
      ttgaaccaa ctggaacgg ctggtgtcg gagacagag 
      cgccaacaa ttggagaaa caactccaa atggtttcg 
      cctcggatc gaaaccatt ccgctggaa ggaggaact 
      gaggcgttt ctggagttg gaggagagc gaggcgagg 
      caaaagcag gagagaccg agagctagc accgggggg 
      aggactaga actgccaag acggccaaa gcttacggc 
      ggaatgcaa ccaccgatg tccgcaacc ccgtccttg 
      agctcggat tcggaagac gacgagttg tttattgac 
      aaagacgag tcagagttg attcagtcg gatgacgag 
      tctttgggg ttggaacta tcggggatg gagttgacc 
      aaaagggtg cagtcggga aaaccggcc gggaacaag 
      ttgaatgat aacagcgat gatgatttt taaaaatgt 
      caaaccaat aaaagctac actttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY