| General information | |||
| TC015228 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 6999212..7003283 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC015228 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05781 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015228 RA
MFKCSQCDN KFTRKDALT RHVKKHKVT TNAPSCSFH QFCEICNSH YRGSRLNHL RSLKHKSVI SAKINDDDD FVEEYQVAF KSRITSYRL KNSQLDDLE VCNFFERNQ DKLITLITR KLREFNQLK INFELFAKY ILPSKDIIE IKSFNTKNI IISISTNIK VELEEICGV IKNKMSEFL EKDSGWILM EILFLELNI NKFNPLRAS TYIPLPKDI AKRRAVINI HNDDNECFK WSVLAHLHS TEVNVNRHR VDAYREFEE ELDFTDISF PVKLTDIFK FEALNNISV NVYGTEQRF DHEKGKWVS DIIGPFYLT SKKRDIHVN LLLIVDDER MIKHYCLIS NISRLISSQ LSKHNHAKF ICNGCLLFF SSEQKLVKH RKYACNEVV TTLPTSDLI INKFGKEVP GNELTFQNF DKSLKVPFV FYFDFESIL KPLRNDNSA DNIRTIVTH LHEPYSFGY YIKCAYNDN LSIYRTYRG KDAPKIFVK WLQDDVNRL YHDHLKHIV PLEMTPLDE LVFQISTHC HLCKEPFKT DYGSLNCDR VRNHCHLTG EFLGPAHSI CNLNYKIPK YIPVFCHNL TNYDSHLFI KALATEKTI INCIAQTKE KYITFSKKI LVDKVMNTT TNTVDNIFI TLRFVDSFR FLSFSLDKL SQTLTSNEC LTIRKHFPD DNQFNLIRQ KGVFPYSYL DCFNKLDDT SLPLIQQFY DNLNKTDIS EHDYERAQE VWNLFDCKT LGDYSDIYL KSDVLLLTD IFENFRKVC LKIYELDPA HYLTAPSLG WDAMLKMTG IKFELLTDI DMVHFFRKS IRGGLCQCS KRKAVANNK FLSNHDPSK PTSFIMYLD ATNLYGAAM SEYLPYSGF RWEDPNEFN DNLILNLTD NADVGYIFE VDLEYPSEL HNLHNDLPF CPEKFIPPS SKTEKLCAT LNNKVHYVL HYRNLRQAI QGGLKLINI HRVLSFNQS PWLKTYIDL NTEMRNKSK NALEKDVFK LMNNSVFGK TMESIDKRV NVKLVTSWN NIGRKPGAE SLIARPNFK NSSIFTPNF VAIQLENEK VFYNKPLYV GFTILDISK TIMYDFYYN FIKLKYGNN ATLLYTDTD SLVIEIFCE NIYDDIKSN IERFDTSNY KAENIHGIP VSSSVIGKM KDEYKGRVI SEFLGTGAK AYCVDVEGE LSKKAKGIK HNVITSELH KIDYQNAVT VPNTEIIKE MNIFRSKLH NIYTELKKK IALSFKDDK RYILNNSEG RTLSWGHKN ILPQTQE
- >TC015228 RA
atgttcaag tgctctcaa tgcgataac aagtttact cgcaaagat gctttaaca cgtcatgta aaaaaacat aaagttact actaacgct ccatcatgt tcatttcat caattttgt gaaatatgc aatagtcac tacagagga tccagatta aaccattta cgttcttta aaacataaa agtgtcatc tccgccaaa attaatgat gatgatgat ttcgttgaa gaatatcaa gttgccttc aaatcaaga attacctca taccgattg aaaaattca caactagat gacttggaa gtgtgcaat ttttttgaa agaaatcaa gataaactt atcacttta ataactaga aaactgaga gaatttaac caacttaaa attaatttc gaacttttt gctaaatat attttacct tctaaagac attatcgaa atcaaatca ttcaacact aaaaatatc atcatttca atatcgaca aatattaag gtagaactg gaagaaata tgtggcgtc ataaaaaac aaaatgagt gaattcttg gaaaaagat agtggttgg attttaatg gaaattttg tttttggaa cttaatatc aacaaattc aatccacta cgtgcatca acatatata ccattaccg aaggacata gcgaagcgg agagcggtt atcaatatt cataatgat gataatgaa tgtttcaaa tggtctgtt ttggcacac cttcattca actgaagta aatgttaat cgacatcga gttgatgct tatcgtgag tttgaagag gaattagat tttacagat atttcattt ccggttaaa ttaactgat atttttaaa tttgaagca cttaataat attagtgta aatgtctat ggaacagag cagcgtttt gatcatgaa aaaggtaaa tgggtatca gatattata ggtcccttt tatttaact tctaagaaa cgagacatt catgtaaat ttattactt atcgtagac gatgaaaga atgatcaaa cattactgt ctgataagc aacatttct cgattaata agttcacaa ctttccaaa cataatcat gctaaattt atatgtaac ggttgtcta ttatttttt tcgagtgaa caaaaatta gttaaacat cgtaagtat gcttgtaat gaagttgtc actacatta ccaacttct gatctgatt ataaataaa tttggtaaa gaagtgcct ggtaatgaa ttaacattt caaaatttt gataaatct cttaaagtt ccttttgta ttttatttc gattttgaa tctatttta aaaccttta cgcaatgat aattcagcc gataatatc cgtacaatt gttacacat ttacatgaa ccatatagt ttcggatat tacataaaa tgtgcatat aatgataat ttatcaatt tatcggact tatcgagga aaagatgct cctaaaatt tttgtgaaa tggttgcaa gatgatgta aatagactt tatcacgat cacctcaaa catatcgta ccacttgaa atgactccg ttagatgaa ttggttttt caaatatct acccattgt catttatgt aaagaacct ttcaaaaca gattacgga agtctaaat tgtgataga gttcgtaat cattgtcac ttaacaggt gaattttta ggacctgca cattctatt tgtaattta aattataag attccaaaa tatatccct gttttttgt cataattta actaattat gatagtcat ttatttatt aaagcttta gcaacagaa aagaccatc attaattgc attgctcaa actaaagaa aagtatatt acattttca aaaaaaatt ttagttgat aaggtcatg aacacgact acaaataca gttgacaat atttttata acgcttaga ttcgtagat tcgttccgt ttcctttct ttttcatta gataaatta tctcagact ttaacttct aatgaatgt ctaaccatt cggaaacac tttccggac gataaccaa tttaatttg ataagacaa aaaggtgtt tttccatat tcatatttg gattgtttt aataagtta gatgatacc agtttacca ttgattcaa caattttat gataatctt aataaaact gatatcagt gaacatgat tatgagcga gcacaggaa gtctggaat ctatttgat tgcaaaacg ttaggtgat tattccgac atttattta aagtctgat gttttgtta ctaacagac atttttgaa aatttcaga aaggtttgt ctcaaaatt tatgaacta gatccagct cattactta acagcacct tcattaggc tgggatgct atgttaaaa atgactgga attaaattc gaattgtta actgatata gacatggta catttcttt cgaaaatct attagagga ggactttgt caatgttca aaacgtaaa gcagtagca aataataag tttttatca aatcatgat ccttctaaa ccaacttct tttataatg taccttgat gcaaccaac ttgtatgga gcagcaatg tctgaatat ttaccttat agcggtttt cgatgggaa gatcctaat gaattcaat gataaccta attctcaat ttgacagat aacgctgat gtaggttat atattcgaa gttgattta gaatatcct tctgaatta cataatctt cacaacgat ttaccattt tgtcctgaa aaatttatt cctccctct agtaaaact gagaaactt tgcgcaacc ttgaacaat aaagtacac tatgtttta cattatcga aatttaaga caagctata caaggtggt ttaaaatta attaatatt caccgtgtt ttaagtttt aatcaatca ccatggttg aaaacatat attgattta aatacagaa atgcgtaac aaatcaaaa aacgcgtta gaaaaagat gtatttaaa ttaatgaat aattcagtt tttggtaaa accatggaa tcaattgat aaaagagta aacgtgaaa ttggtaaca tcatggaat aatataggt agaaaacca ggtgctgaa agtttaatt gcacgacca aattttaaa aattcatct atctttaca ccaaatttt gtagctatt caattagag aacgaaaaa gttttctac aataaacct ctgtatgta ggttttact attctagat atcagtaaa actattatg tatgatttt tattataat ttcataaaa cttaaatac ggtaacaat gcaacgctt ttgtatacc gatactgat agtttagta attgaaatt ttttgtgaa aatatttat gatgatatt aaatctaat atagagcgt tttgatact tcaaattat aaagcagaa aatattcat ggaatacct gtatcgtct tctgtcatc gggaagatg aaagatgaa tataaagga cgtgtaata tcagaattt ttaggaaca ggtgctaaa gcatattgt gtggatgtt gaaggtgaa ttatctaaa aaagctaag ggtattaaa cacaatgta attacaagt gaattacat aaaattgac taccaaaac gcagttact gttccaaac actgaaatt attaaagaa atgaatatc tttcgttcg aagcttcat aacatttac acagaatta aagaaaaaa attgcttta tctttcaaa gatgataaa cgttacatc ttaaacaat tctgaaggt agaacatta tcttggggt cataaaaat attcttcct caaactcag gaataa
- >TC015228 RA
atgttcaag tgctctcaa tgcgataac aagtttact cgcaaagat gctttaaca cgtcatgta aaaaaacat aaagttact actaacgct ccatcatgt tcatttcat caattttgt gaaatatgc aatagtcac tacagagga tccagatta aaccattta cgttcttta aaacataaa agtgtcatc tccgccaaa attaatgat gatgatgat ttcgttgaa gaatatcaa gttgccttc aaatcaaga attacctca taccgattg aaaaattca caactagat gacttggaa gtgtgcaat ttttttgaa agaaatcaa gataaactt atcacttta ataactaga aaactgaga gaatttaac caacttaaa attaatttc gaacttttt gctaaatat attttacct tctaaagac attatcgaa atcaaatca ttcaacact aaaaatatc atcatttca atatcgaca aatattaag gtagaactg gaagaaata tgtggcgtc ataaaaaac aaaatgagt gaattcttg gaaaaagat agtggttgg attttaatg gaaattttg tttttggaa cttaatatc aacaaattc aatccacta cgtgcatca acatatata ccattaccg aaggacata gcgaagcgg agagcggtt atcaatatt cataatgat gataatgaa tgtttcaaa tggtctgtt ttggcacac cttcattca actgaagta aatgttaat cgacatcga gttgatgct tatcgtgag tttgaagag gaattagat tttacagat atttcattt ccggttaaa ttaactgat atttttaaa tttgaagca cttaataat attagtgta aatgtctat ggaacagag cagcgtttt gatcatgaa aaaggtaaa tgggtatca gatattata ggtcccttt tatttaact tctaagaaa cgagacatt catgtaaat ttattactt atcgtagac gatgaaaga atgatcaaa cattactgt ctgataagc aacatttct cgattaata agttcacaa ctttccaaa cataatcat gctaaattt atatgtaac ggttgtcta ttatttttt tcgagtgaa caaaaatta gttaaacat cgtaagtat gcttgtaat gaagttgtc actacatta ccaacttct gatctgatt ataaataaa tttggtaaa gaagtgcct ggtaatgaa ttaacattt caaaatttt gataaatct cttaaagtt ccttttgta ttttatttc gattttgaa tctatttta aaaccttta cgcaatgat aattcagcc gataatatc cgtacaatt gttacacat ttacatgaa ccatatagt ttcggatat tacataaaa tgtgcatat aatgataat ttatcaatt tatcggact tatcgagga aaagatgct cctaaaatt tttgtgaaa tggttgcaa gatgatgta aatagactt tatcacgat cacctcaaa catatcgta ccacttgaa atgactccg ttagatgaa ttggttttt caaatatct acccattgt catttatgt aaagaacct ttcaaaaca gattacgga agtctaaat tgtgataga gttcgtaat cattgtcac ttaacaggt gaattttta ggacctgca cattctatt tgtaattta aattataag attccaaaa tatatccct gttttttgt cataattta actaattat gatagtcat ttatttatt aaagcttta gcaacagaa aagaccatc attaattgc attgctcaa actaaagaa aagtatatt acattttca aaaaaaatt ttagttgat aaggtcatg aacacgact acaaataca gttgacaat atttttata acgcttaga ttcgtagat tcgttccgt ttcctttct ttttcatta gataaatta tctcagact ttaacttct aatgaatgt ctaaccatt cggaaacac tttccggac gataaccaa tttaatttg ataagacaa aaaggtgtt tttccatat tcatatttg gattgtttt aataagtta gatgatacc agtttacca ttgattcaa caattttat gataatctt aataaaact gatatcagt gaacatgat tatgagcga gcacaggaa gtctggaat ctatttgat tgcaaaacg ttaggtgat tattccgac atttattta aagtctgat gttttgtta ctaacagac atttttgaa aatttcaga aaggtttgt ctcaaaatt tatgaacta gatccagct cattactta acagcacct tcattaggc tgggatgct atgttaaaa atgactgga attaaattc gaattgtta actgatata gacatggta catttcttt cgaaaatct attagagga ggactttgt caatgttca aaacgtaaa gcagtagca aataataag tttttatca aatcatgat ccttctaaa ccaacttct tttataatg taccttgat gcaaccaac ttgtatgga gcagcaatg tctgaatat ttaccttat agcggtttt cgatgggaa gatcctaat gaattcaat gataaccta attctcaat ttgacagat aacgctgat gtaggttat atattcgaa gttgattta gaatatcct tctgaatta cataatctt cacaacgat ttaccattt tgtcctgaa aaatttatt cctccctct agtaaaact gagaaactt tgcgcaacc ttgaacaat aaagtacac tatgtttta cattatcga aatttaaga caagctata caaggtggt ttaaaatta attaatatt caccgtgtt ttaagtttt aatcaatca ccatggttg aaaacatat attgattta aatacagaa atgcgtaac aaatcaaaa aacgcgtta gaaaaagat gtatttaaa ttaatgaat aattcagtt tttggtaaa accatggaa tcaattgat aaaagagta aacgtgaaa ttggtaaca tcatggaat aatataggt agaaaacca ggtgctgaa agtttaatt gcacgacca aattttaaa aattcatct atctttaca ccaaatttt gtagctatt caattagag aacgaaaaa gttttctac aataaacct ctgtatgta ggttttact attctagat atcagtaaa actattatg tatgatttt tattataat ttcataaaa cttaaatac ggtaacaat gcaacgctt ttgtatacc gatactgat agtttagta attgaaatt ttttgtgaa aatatttat gatgatatt aaatctaat atagagcgt tttgatact tcaaattat aaagcagaa aatattcat ggaatacct gtatcgtct tctgtcatc gggaagatg aaagatgaa tataaagga cgtgtaata tcagaattt ttaggaaca ggtgctaaa gcatattgt gtggatgtt gaaggtgaa ttatctaaa aaagctaag ggtattaaa cacaatgta attacaagt gaattacat aaaattgac taccaaaac gcagttact gttccaaac actgaaatt attaaagaa atgaatatc tttcgttcg aagcttcat aacatttac acagaatta aagaaaaaa attgcttta tctttcaaa gatgataaa cgttacatc ttaaacaat tctgaaggt agaacatta tcttggggt cataaaaat attcttcct caaactcag gaataa
