Status
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Gene information
General information
TC015228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6999212..7003283
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015228
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05781

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015228 RA
        MFKCSQCDN KFTRKDALT RHVKKHKVT TNAPSCSFH 
        QFCEICNSH YRGSRLNHL RSLKHKSVI SAKINDDDD 
        FVEEYQVAF KSRITSYRL KNSQLDDLE VCNFFERNQ 
        DKLITLITR KLREFNQLK INFELFAKY ILPSKDIIE 
        IKSFNTKNI IISISTNIK VELEEICGV IKNKMSEFL 
        EKDSGWILM EILFLELNI NKFNPLRAS TYIPLPKDI 
        AKRRAVINI HNDDNECFK WSVLAHLHS TEVNVNRHR 
        VDAYREFEE ELDFTDISF PVKLTDIFK FEALNNISV 
        NVYGTEQRF DHEKGKWVS DIIGPFYLT SKKRDIHVN 
        LLLIVDDER MIKHYCLIS NISRLISSQ LSKHNHAKF 
        ICNGCLLFF SSEQKLVKH RKYACNEVV TTLPTSDLI 
        INKFGKEVP GNELTFQNF DKSLKVPFV FYFDFESIL 
        KPLRNDNSA DNIRTIVTH LHEPYSFGY YIKCAYNDN 
        LSIYRTYRG KDAPKIFVK WLQDDVNRL YHDHLKHIV 
        PLEMTPLDE LVFQISTHC HLCKEPFKT DYGSLNCDR 
        VRNHCHLTG EFLGPAHSI CNLNYKIPK YIPVFCHNL 
        TNYDSHLFI KALATEKTI INCIAQTKE KYITFSKKI 
        LVDKVMNTT TNTVDNIFI TLRFVDSFR FLSFSLDKL 
        SQTLTSNEC LTIRKHFPD DNQFNLIRQ KGVFPYSYL 
        DCFNKLDDT SLPLIQQFY DNLNKTDIS EHDYERAQE 
        VWNLFDCKT LGDYSDIYL KSDVLLLTD IFENFRKVC 
        LKIYELDPA HYLTAPSLG WDAMLKMTG IKFELLTDI 
        DMVHFFRKS IRGGLCQCS KRKAVANNK FLSNHDPSK 
        PTSFIMYLD ATNLYGAAM SEYLPYSGF RWEDPNEFN 
        DNLILNLTD NADVGYIFE VDLEYPSEL HNLHNDLPF 
        CPEKFIPPS SKTEKLCAT LNNKVHYVL HYRNLRQAI 
        QGGLKLINI HRVLSFNQS PWLKTYIDL NTEMRNKSK 
        NALEKDVFK LMNNSVFGK TMESIDKRV NVKLVTSWN 
        NIGRKPGAE SLIARPNFK NSSIFTPNF VAIQLENEK 
        VFYNKPLYV GFTILDISK TIMYDFYYN FIKLKYGNN 
        ATLLYTDTD SLVIEIFCE NIYDDIKSN IERFDTSNY 
        KAENIHGIP VSSSVIGKM KDEYKGRVI SEFLGTGAK 
        AYCVDVEGE LSKKAKGIK HNVITSELH KIDYQNAVT 
        VPNTEIIKE MNIFRSKLH NIYTELKKK IALSFKDDK 
        RYILNNSEG RTLSWGHKN ILPQTQE
      CDS sequence
      • >TC015228 RA
        atgttcaag tgctctcaa tgcgataac aagtttact 
        cgcaaagat gctttaaca cgtcatgta aaaaaacat 
        aaagttact actaacgct ccatcatgt tcatttcat 
        caattttgt gaaatatgc aatagtcac tacagagga 
        tccagatta aaccattta cgttcttta aaacataaa 
        agtgtcatc tccgccaaa attaatgat gatgatgat 
        ttcgttgaa gaatatcaa gttgccttc aaatcaaga 
        attacctca taccgattg aaaaattca caactagat 
        gacttggaa gtgtgcaat ttttttgaa agaaatcaa 
        gataaactt atcacttta ataactaga aaactgaga 
        gaatttaac caacttaaa attaatttc gaacttttt 
        gctaaatat attttacct tctaaagac attatcgaa 
        atcaaatca ttcaacact aaaaatatc atcatttca 
        atatcgaca aatattaag gtagaactg gaagaaata 
        tgtggcgtc ataaaaaac aaaatgagt gaattcttg 
        gaaaaagat agtggttgg attttaatg gaaattttg 
        tttttggaa cttaatatc aacaaattc aatccacta 
        cgtgcatca acatatata ccattaccg aaggacata 
        gcgaagcgg agagcggtt atcaatatt cataatgat 
        gataatgaa tgtttcaaa tggtctgtt ttggcacac 
        cttcattca actgaagta aatgttaat cgacatcga 
        gttgatgct tatcgtgag tttgaagag gaattagat 
        tttacagat atttcattt ccggttaaa ttaactgat 
        atttttaaa tttgaagca cttaataat attagtgta 
        aatgtctat ggaacagag cagcgtttt gatcatgaa 
        aaaggtaaa tgggtatca gatattata ggtcccttt 
        tatttaact tctaagaaa cgagacatt catgtaaat 
        ttattactt atcgtagac gatgaaaga atgatcaaa 
        cattactgt ctgataagc aacatttct cgattaata 
        agttcacaa ctttccaaa cataatcat gctaaattt 
        atatgtaac ggttgtcta ttatttttt tcgagtgaa 
        caaaaatta gttaaacat cgtaagtat gcttgtaat 
        gaagttgtc actacatta ccaacttct gatctgatt 
        ataaataaa tttggtaaa gaagtgcct ggtaatgaa 
        ttaacattt caaaatttt gataaatct cttaaagtt 
        ccttttgta ttttatttc gattttgaa tctatttta 
        aaaccttta cgcaatgat aattcagcc gataatatc 
        cgtacaatt gttacacat ttacatgaa ccatatagt 
        ttcggatat tacataaaa tgtgcatat aatgataat 
        ttatcaatt tatcggact tatcgagga aaagatgct 
        cctaaaatt tttgtgaaa tggttgcaa gatgatgta 
        aatagactt tatcacgat cacctcaaa catatcgta 
        ccacttgaa atgactccg ttagatgaa ttggttttt 
        caaatatct acccattgt catttatgt aaagaacct 
        ttcaaaaca gattacgga agtctaaat tgtgataga 
        gttcgtaat cattgtcac ttaacaggt gaattttta 
        ggacctgca cattctatt tgtaattta aattataag 
        attccaaaa tatatccct gttttttgt cataattta 
        actaattat gatagtcat ttatttatt aaagcttta 
        gcaacagaa aagaccatc attaattgc attgctcaa 
        actaaagaa aagtatatt acattttca aaaaaaatt 
        ttagttgat aaggtcatg aacacgact acaaataca 
        gttgacaat atttttata acgcttaga ttcgtagat 
        tcgttccgt ttcctttct ttttcatta gataaatta 
        tctcagact ttaacttct aatgaatgt ctaaccatt 
        cggaaacac tttccggac gataaccaa tttaatttg 
        ataagacaa aaaggtgtt tttccatat tcatatttg 
        gattgtttt aataagtta gatgatacc agtttacca 
        ttgattcaa caattttat gataatctt aataaaact 
        gatatcagt gaacatgat tatgagcga gcacaggaa 
        gtctggaat ctatttgat tgcaaaacg ttaggtgat 
        tattccgac atttattta aagtctgat gttttgtta 
        ctaacagac atttttgaa aatttcaga aaggtttgt 
        ctcaaaatt tatgaacta gatccagct cattactta 
        acagcacct tcattaggc tgggatgct atgttaaaa 
        atgactgga attaaattc gaattgtta actgatata 
        gacatggta catttcttt cgaaaatct attagagga 
        ggactttgt caatgttca aaacgtaaa gcagtagca 
        aataataag tttttatca aatcatgat ccttctaaa 
        ccaacttct tttataatg taccttgat gcaaccaac 
        ttgtatgga gcagcaatg tctgaatat ttaccttat 
        agcggtttt cgatgggaa gatcctaat gaattcaat 
        gataaccta attctcaat ttgacagat aacgctgat 
        gtaggttat atattcgaa gttgattta gaatatcct 
        tctgaatta cataatctt cacaacgat ttaccattt 
        tgtcctgaa aaatttatt cctccctct agtaaaact 
        gagaaactt tgcgcaacc ttgaacaat aaagtacac 
        tatgtttta cattatcga aatttaaga caagctata 
        caaggtggt ttaaaatta attaatatt caccgtgtt 
        ttaagtttt aatcaatca ccatggttg aaaacatat 
        attgattta aatacagaa atgcgtaac aaatcaaaa 
        aacgcgtta gaaaaagat gtatttaaa ttaatgaat 
        aattcagtt tttggtaaa accatggaa tcaattgat 
        aaaagagta aacgtgaaa ttggtaaca tcatggaat 
        aatataggt agaaaacca ggtgctgaa agtttaatt 
        gcacgacca aattttaaa aattcatct atctttaca 
        ccaaatttt gtagctatt caattagag aacgaaaaa 
        gttttctac aataaacct ctgtatgta ggttttact 
        attctagat atcagtaaa actattatg tatgatttt 
        tattataat ttcataaaa cttaaatac ggtaacaat 
        gcaacgctt ttgtatacc gatactgat agtttagta 
        attgaaatt ttttgtgaa aatatttat gatgatatt 
        aaatctaat atagagcgt tttgatact tcaaattat 
        aaagcagaa aatattcat ggaatacct gtatcgtct 
        tctgtcatc gggaagatg aaagatgaa tataaagga 
        cgtgtaata tcagaattt ttaggaaca ggtgctaaa 
        gcatattgt gtggatgtt gaaggtgaa ttatctaaa 
        aaagctaag ggtattaaa cacaatgta attacaagt 
        gaattacat aaaattgac taccaaaac gcagttact 
        gttccaaac actgaaatt attaaagaa atgaatatc 
        tttcgttcg aagcttcat aacatttac acagaatta 
        aagaaaaaa attgcttta tctttcaaa gatgataaa 
        cgttacatc ttaaacaat tctgaaggt agaacatta 
        tcttggggt cataaaaat attcttcct caaactcag 
        gaataa
      mRNA sequence
      • >TC015228 RA
        atgttcaag tgctctcaa tgcgataac aagtttact 
        cgcaaagat gctttaaca cgtcatgta aaaaaacat 
        aaagttact actaacgct ccatcatgt tcatttcat 
        caattttgt gaaatatgc aatagtcac tacagagga 
        tccagatta aaccattta cgttcttta aaacataaa 
        agtgtcatc tccgccaaa attaatgat gatgatgat 
        ttcgttgaa gaatatcaa gttgccttc aaatcaaga 
        attacctca taccgattg aaaaattca caactagat 
        gacttggaa gtgtgcaat ttttttgaa agaaatcaa 
        gataaactt atcacttta ataactaga aaactgaga 
        gaatttaac caacttaaa attaatttc gaacttttt 
        gctaaatat attttacct tctaaagac attatcgaa 
        atcaaatca ttcaacact aaaaatatc atcatttca 
        atatcgaca aatattaag gtagaactg gaagaaata 
        tgtggcgtc ataaaaaac aaaatgagt gaattcttg 
        gaaaaagat agtggttgg attttaatg gaaattttg 
        tttttggaa cttaatatc aacaaattc aatccacta 
        cgtgcatca acatatata ccattaccg aaggacata 
        gcgaagcgg agagcggtt atcaatatt cataatgat 
        gataatgaa tgtttcaaa tggtctgtt ttggcacac 
        cttcattca actgaagta aatgttaat cgacatcga 
        gttgatgct tatcgtgag tttgaagag gaattagat 
        tttacagat atttcattt ccggttaaa ttaactgat 
        atttttaaa tttgaagca cttaataat attagtgta 
        aatgtctat ggaacagag cagcgtttt gatcatgaa 
        aaaggtaaa tgggtatca gatattata ggtcccttt 
        tatttaact tctaagaaa cgagacatt catgtaaat 
        ttattactt atcgtagac gatgaaaga atgatcaaa 
        cattactgt ctgataagc aacatttct cgattaata 
        agttcacaa ctttccaaa cataatcat gctaaattt 
        atatgtaac ggttgtcta ttatttttt tcgagtgaa 
        caaaaatta gttaaacat cgtaagtat gcttgtaat 
        gaagttgtc actacatta ccaacttct gatctgatt 
        ataaataaa tttggtaaa gaagtgcct ggtaatgaa 
        ttaacattt caaaatttt gataaatct cttaaagtt 
        ccttttgta ttttatttc gattttgaa tctatttta 
        aaaccttta cgcaatgat aattcagcc gataatatc 
        cgtacaatt gttacacat ttacatgaa ccatatagt 
        ttcggatat tacataaaa tgtgcatat aatgataat 
        ttatcaatt tatcggact tatcgagga aaagatgct 
        cctaaaatt tttgtgaaa tggttgcaa gatgatgta 
        aatagactt tatcacgat cacctcaaa catatcgta 
        ccacttgaa atgactccg ttagatgaa ttggttttt 
        caaatatct acccattgt catttatgt aaagaacct 
        ttcaaaaca gattacgga agtctaaat tgtgataga 
        gttcgtaat cattgtcac ttaacaggt gaattttta 
        ggacctgca cattctatt tgtaattta aattataag 
        attccaaaa tatatccct gttttttgt cataattta 
        actaattat gatagtcat ttatttatt aaagcttta 
        gcaacagaa aagaccatc attaattgc attgctcaa 
        actaaagaa aagtatatt acattttca aaaaaaatt 
        ttagttgat aaggtcatg aacacgact acaaataca 
        gttgacaat atttttata acgcttaga ttcgtagat 
        tcgttccgt ttcctttct ttttcatta gataaatta 
        tctcagact ttaacttct aatgaatgt ctaaccatt 
        cggaaacac tttccggac gataaccaa tttaatttg 
        ataagacaa aaaggtgtt tttccatat tcatatttg 
        gattgtttt aataagtta gatgatacc agtttacca 
        ttgattcaa caattttat gataatctt aataaaact 
        gatatcagt gaacatgat tatgagcga gcacaggaa 
        gtctggaat ctatttgat tgcaaaacg ttaggtgat 
        tattccgac atttattta aagtctgat gttttgtta 
        ctaacagac atttttgaa aatttcaga aaggtttgt 
        ctcaaaatt tatgaacta gatccagct cattactta 
        acagcacct tcattaggc tgggatgct atgttaaaa 
        atgactgga attaaattc gaattgtta actgatata 
        gacatggta catttcttt cgaaaatct attagagga 
        ggactttgt caatgttca aaacgtaaa gcagtagca 
        aataataag tttttatca aatcatgat ccttctaaa 
        ccaacttct tttataatg taccttgat gcaaccaac 
        ttgtatgga gcagcaatg tctgaatat ttaccttat 
        agcggtttt cgatgggaa gatcctaat gaattcaat 
        gataaccta attctcaat ttgacagat aacgctgat 
        gtaggttat atattcgaa gttgattta gaatatcct 
        tctgaatta cataatctt cacaacgat ttaccattt 
        tgtcctgaa aaatttatt cctccctct agtaaaact 
        gagaaactt tgcgcaacc ttgaacaat aaagtacac 
        tatgtttta cattatcga aatttaaga caagctata 
        caaggtggt ttaaaatta attaatatt caccgtgtt 
        ttaagtttt aatcaatca ccatggttg aaaacatat 
        attgattta aatacagaa atgcgtaac aaatcaaaa 
        aacgcgtta gaaaaagat gtatttaaa ttaatgaat 
        aattcagtt tttggtaaa accatggaa tcaattgat 
        aaaagagta aacgtgaaa ttggtaaca tcatggaat 
        aatataggt agaaaacca ggtgctgaa agtttaatt 
        gcacgacca aattttaaa aattcatct atctttaca 
        ccaaatttt gtagctatt caattagag aacgaaaaa 
        gttttctac aataaacct ctgtatgta ggttttact 
        attctagat atcagtaaa actattatg tatgatttt 
        tattataat ttcataaaa cttaaatac ggtaacaat 
        gcaacgctt ttgtatacc gatactgat agtttagta 
        attgaaatt ttttgtgaa aatatttat gatgatatt 
        aaatctaat atagagcgt tttgatact tcaaattat 
        aaagcagaa aatattcat ggaatacct gtatcgtct 
        tctgtcatc gggaagatg aaagatgaa tataaagga 
        cgtgtaata tcagaattt ttaggaaca ggtgctaaa 
        gcatattgt gtggatgtt gaaggtgaa ttatctaaa 
        aaagctaag ggtattaaa cacaatgta attacaagt 
        gaattacat aaaattgac taccaaaac gcagttact 
        gttccaaac actgaaatt attaaagaa atgaatatc 
        tttcgttcg aagcttcat aacatttac acagaatta 
        aagaaaaaa attgcttta tctttcaaa gatgataaa 
        cgttacatc ttaaacaat tctgaaggt agaacatta 
        tcttggggt cataaaaat attcttcct caaactcag 
        gaataa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY