Status
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Gene information
General information
TC034086
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015227,TC015225
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7003760..7021934
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034086
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05780

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034086 RA
      MTIAEVTIT QGKLRGTTS CDINGDTYF RFQGIPYAK 
      PPVGVLRFK APQPPENWT GVLDATKEG DPCYGRHFF 
      KKNLIVGSE NCLVLNVYT KNLRTDTNR ITQPVLFWI 
      HGGDFVTGS GTSEMYGPD YLMSENVVL VTINYRLGM 
      LGFLSFEDV SLGVPGNAG FKDMVMALK WVQKNIIMF 
      GGDPHNVTI FGSGSGAMS VHLLHLSPI SKTLFHKAI 
      AQSGTVLSL RARGTLAIS QIASILKLK DERSVYKHL 
      MALKVAEIY KLQKTLLET CGPFDRNLF GYVVEKKSD 
      EPAFLKEDP FKIIASGNF NQVPLICGY VANETIGSE 
      LNQGPTFEE KIIPWFFGY EFGSLESYT VAKEIKSFY 
      YGSEETLPR DKTKTTTLI SDAQTVFEI QTTVRKHYV 
      LSRAPTYLY RVSLKTALK IPGLPADVN YIFKNFATS 
      IKPGTKEHK VMKRFVRLW CNFAKYAHP TPRRDDALL 
      NVIWRFVSN DDELDFLDI GDELVASAN PDAQRMKFW 
      KKLFNKSPA NGKLIQYKR MTFIQSHKP KMEPLVTTV 
      QGQLKGTTS LDSSGEKYY KFQGIPFAK PPVGLLRFK 
      APEPPEKWT GVLDATKEG SPCYARHPF NNKIVGSED 
      CLYLNVYTK KIASNNCSL SPVLFWIHG GAFLNGSGG 
      TEMYGPDFL ITQDVVVVT INYRLGILG FLSLENPDL 
      KVPGNAGLK DIVMALKWV QTNIKNFGG DPNNVTVFG 
      QSAGGVAVH LLLLSQMSR GLFHKAIAQ SGCAFSPWA 
      RGTTGVKEL APVLHVEED EEKIYERLM KLPVDELFK 
      LQEKIVSFL NIYASKHRA FAYVVEKIS DGFLSEEPI 
      DIISNGKFQ QLPFMIGYT TAETWGFDS LRPDDWPGA 
      KNLEDSIPW TLGFKTGSP ESQVMTEKI KKFYCGGHI 
      PTTIKLETK IAIHTDANF VFGVYKTAL SHFSKSQAP 
      TYLYRFSAE TELNVMRQF YKIKIPGVV HCDDIGYLF 
      KNVASPRIK PGSKEEVLL KKVVKLWTN FAKFGNPTP 
      DNSLGVVWK PVTDKEVDY LDIGESLTP ARNSIEHKR 
      VQFLTKLYE RKNSSSHLV KTNNKQYDT GSQQQQQQQ 
      QQQQQQQQQ QPTAFQV
    CDS sequence
    • >TC034086 RA
      atgactatc gcggaagta acaatcacc caaggcaaa 
      ttacgcggt accacttct tgtgacata aacggggac 
      acctacttc cgctttcaa ggcattcca tacgctaag 
      ccgcctgtg ggggtactc agatttaag gccccccaa 
      ccacctgaa aactggacg ggtgttttg gatgccacc 
      aaagaaggt gatccttgc tatggtcgt cattttttc 
      aagaagaat ttaattgtg ggaagcgaa aattgtttg 
      gtacttaat gtttacacc aaaaaccta cgcactgac 
      accaaccgt atcacccaa cctgtgctt ttctggatc 
      cacggtggg gattttgtg actggaagt ggtaccagt 
      gaaatgtac ggtcctgac tatctaatg agcgaaaat 
      gtggtactt gtcacaatt aattatcgg ttggggatg 
      ttggggttc ctaagcttt gaggatgtg tctttagga 
      gtaccgggt aatgctggg ttcaaggac atggtaatg 
      gccttgaaa tgggttcag aaaaatatt ataatgttt 
      ggtggcgat ccacacaat gtgacgatt ttcgggtct 
      gggtccggt gccatgtca gtgcatctt ttacactta 
      tcgccaatc agtaaaacc ctctttcat aaagctata 
      gcccagagt ggtaccgtt ttgagtttg agggcccgt 
      ggtactctt gccatttca caaatagct tcgattttg 
      aaattaaag gatgagaga agtgtttac aagcacttg 
      atggcactg aaagtcgcc gagatttac aaacttcaa 
      aaaacttta cttgaaact tgtggtcca tttgaccgc 
      aatcttttt ggttacgtc gttgagaaa aaatcagac 
      gagcccgct ttcttgaag gaagatcct ttcaaaatc 
      attgcttcg ggcaatttt aaccaggta ccactaata 
      tgtgggtac gttgccaac gaaaccatt ggttccgaa 
      ctcaatcaa gggccaacc tttgaggag aaaatcatc 
      ccttggttt tttggttac gagtttggt agtctagaa 
      agctacacg gtggccaaa gaaatcaaa agtttttat 
      tacgggtct gaggaaact ctaccaagg gataaaacc 
      aaaactacg actttgatt tccgacgcc cagacggtt 
      ttcgaaatt caaacaacc gtcagaaaa cattacgtt 
      ttatcccga gcccctacc tatttatac cgagtgtcc 
      ttaaagact gctttgaaa ataccgggg cttccagcc 
      gatgtcaac tacattttc aaaaatttt gccacaagt 
      atcaagcct ggaactaaa gaacacaaa gtgatgaag 
      cggttcgtt cggttgtgg tgcaatttt gccaaatat 
      gcacacccg acgcctcgg agagacgac gcattgctg 
      aatgttatt tggaggttt gttagcaat gacgacgag 
      ttagatttt ctggatatt ggtgacgag ttggtcgct 
      tcggccaat cccgacgcc cagcgaatg aagttctgg 
      aaaaagttg tttaacaag agcccagct aatggaaaa 
      ctaatccaa tataaacgc atgactttc attcaatca 
      cacaaacct aaaatggaa cccctagtc acaacagtg 
      caaggacag ttaaaaggt accacttct cttgattcg 
      tccggagag aagtactac aagtttcaa ggcattccg 
      tttgctaaa ccacctgtg ggccttctc cgatttaag 
      gcccctgaa cctcccgaa aaatggact ggggtactg 
      gacgccaca aaggaaggg agcccttgt tacgcccgc 
      cacccgttt aacaataaa attgtcggg agtgaagac 
      tgcctttat ttgaacgtt tacacgaaa aaaatagca 
      agtaacaac tgttctttg tctcctgtc ctgttttgg 
      atccacgga ggtgcgttc ttgaacggt tccggagga 
      acggaaatg tacggtccc gatttcctc atcactcaa 
      gatgtggtc gtggtaacg atcaactac cgactaggg 
      attttgggc tttttgagc cttgaaaac cccgatttg 
      aaagtacca ggcaacgcc ggcttgaag gacatagta 
      atggccttg aaatgggtc caaaccaac atcaaaaac 
      ttcggtggt gaccctaat aacgtcacc gtttttggg 
      caaagtgcg ggtggagtt gccgtccat ctcctgctt 
      ttgtcccaa atgagtcgc gggttgttc cacaaggct 
      atagcccag agcgggtgt gcgtttagc ccttgggca 
      cgtggtacc accggtgtt aaagaattg gccccggta 
      ctacacgtg gaagaagac gaagagaag atttacgag 
      cgtttgatg aaactacca gttgacgaa ttgttcaaa 
      ctccaggag aaaattgtc tcgtttcta aatatttac 
      gcaagcaaa catagagcc tttgcctac gttgtggag 
      aagatttcg gatggtttt ctaagcgag gaaccaatt 
      gatattatt tcgaatgga aaattccaa caactgcct 
      ttcatgatt gggtacacg actgccgaa acttggggc 
      tttgactcg ctcaggcct gacgattgg ccaggggcg 
      aaaaatctg gaagactca atcccctgg actctagga 
      tttaaaact ggaagcccg gaaagtcaa gtcatgacg 
      gagaaaata aaaaagttc tattgcggc ggacatatt 
      ccgaccacc attaaatta gagacaaaa attgcgatc 
      cacaccgat gcaaatttc gttttcggg gtttataaa 
      acggctcta agccacttc agcaagtca caagccccc 
      acttatttg tacaggttt tcggcagag actgaactg 
      aatgtgatg cggcaattc tacaagatc aaaattcca 
      ggtgtggtc cattgtgac gatattggt tatctgttc 
      aaaaacgtg gcttcgcct cgcatcaaa cccggttct 
      aaggaagaa gttttgttg aaaaaggtc gtaaagttg 
      tggacgaat tttgccaaa ttcggcaat cccacacct 
      gacaactcc ttgggtgtt gtctggaaa cccgttact 
      gacaaagaa gttgattat ttggacatt ggcgagagt 
      ctgacgccg gctagaaac tcgattgag cacaagaga 
      gtacaattt ttgacaaaa ctgtacgag cgcaagaac 
      tcctcgtcc catttggta aaaacaaac aataaacaa 
      tatgataca ggaagccaa caacaacaa caacaacaa 
      caacaacaa caacaacaa caacaacaa caacccact 
      gcgtttcaa gtgtaa
    mRNA sequence
    • >TC034086 RA
      tttaatcct aaaaaatga ctatcgcgg aagtaacaa 
      tcacccaag gcaaattac gcggtacca cttcttgtg 
      acataaacg gggacacct acttccgct ttcaaggca 
      ttccatacg ctaagccgc ctgtggggg tactcagat 
      ttaaggccc cccaaccac ctgaaaact ggacgggtg 
      ttttggatg ccaccaaag aaggtgatc cttgctatg 
      gtcgtcatt ttttcaaga agaatttaa ttgtgggaa 
      gcgaaaatt gtttggtac ttaatgttt acaccaaaa 
      acctacgca ctgacacca accgtatca cccaacctg 
      tgcttttct ggatccacg gtggggatt ttgtgactg 
      gaagtggta ccagtgaaa tgtacggtc ctgactatc 
      taatgagcg aaaatgtgg tacttgtca caattaatt 
      atcggttgg ggatgttgg ggttcctaa gctttgagg 
      atgtgtctt taggagtac cgggtaatg ctgggttca 
      aggacatgg taatggcct tgaaatggg ttcagaaaa 
      atattataa tgtttggtg gcgatccac acaatgtga 
      cgattttcg ggtctgggt ccggtgcca tgtcagtgc 
      atcttttac acttatcgc caatcagta aaaccctct 
      ttcataaag ctatagccc agagtggta ccgttttga 
      gtttgaggg cccgtggta ctcttgcca tttcacaaa 
      tagcttcga ttttgaaat taaaggatg agagaagtg 
      tttacaagc acttgatgg cactgaaag tcgccgaga 
      tttacaaac ttcaaaaaa ctttacttg aaacttgtg 
      gtccatttg accgcaatc tttttggtt acgtcgttg 
      agaaaaaat cagacgagc ccgctttct tgaaggaag 
      atcctttca aaatcattg cttcgggca attttaacc 
      aggtaccac taatatgtg ggtacgttg ccaacgaaa 
      ccattggtt ccgaactca atcaagggc caacctttg 
      aggagaaaa tcatccctt ggttttttg gttacgagt 
      ttggtagtc tagaaagct acacggtgg ccaaagaaa 
      tcaaaagtt tttattacg ggtctgagg aaactctac 
      caagggata aaaccaaaa ctacgactt tgatttccg 
      acgcccaga cggttttcg aaattcaaa caaccgtca 
      gaaaacatt acgttttat cccgagccc ctacctatt 
      tataccgag tgtccttaa agactgctt tgaaaatac 
      cggggcttc cagccgatg tcaactaca ttttcaaaa 
      attttgcca caagtatca agcctggaa ctaaagaac 
      acaaagtga tgaagcggt tcgttcggt tgtggtgca 
      attttgcca aatatgcac acccgacgc ctcggagag 
      acgacgcat tgctgaatg ttatttgga ggtttgtta 
      gcaatgacg acgagttag attttctgg atattggtg 
      acgagttgg tcgcttcgg ccaatcccg acgcccagc 
      gaatgaagt tctggaaaa agttgttta acaagagcc 
      cagctaatg gaaaactaa tccaatata aacgcatga 
      ctttcattc aatcacaca aacctaaaa tggaacccc 
      tagtcacaa cagtgcaag gacagttaa aaggtacca 
      cttctcttg attcgtccg gagagaagt actacaagt 
      ttcaaggca ttccgtttg ctaaaccac ctgtgggcc 
      ttctccgat ttaaggccc ctgaacctc ccgaaaaat 
      ggactgggg tactggacg ccacaaagg aagggagcc 
      cttgttacg cccgccacc cgtttaaca ataaaattg 
      tcgggagtg aagactgcc tttatttga acgtttaca 
      cgaaaaaaa tagcaagta acaactgtt ctttgtctc 
      ctgtcctgt tttggatcc acggaggtg cgttcttga 
      acggttccg gaggaacgg aaatgtacg gtcccgatt 
      tcctcatca ctcaagatg tggtcgtgg taacgatca 
      actaccgac tagggattt tgggctttt tgagccttg 
      aaaaccccg atttgaaag taccaggca acgccggct 
      tgaaggaca tagtaatgg ccttgaaat gggtccaaa 
      ccaacatca aaaacttcg gtggtgacc ctaataacg 
      tcaccgttt ttgggcaaa gtgcgggtg gagttgccg 
      tccatctcc tgcttttgt cccaaatga gtcgcgggt 
      tgttccaca aggctatag cccagagcg ggtgtgcgt 
      ttagccctt gggcacgtg gtaccaccg gtgttaaag 
      aattggccc cggtactac acgtggaag aagacgaag 
      agaagattt acgagcgtt tgatgaaac taccagttg 
      acgaattgt tcaaactcc aggagaaaa ttgtctcgt 
      ttctaaata tttacgcaa gcaaacata gagcctttg 
      cctacgttg tggagaaga tttcggatg gttttctaa 
      gcgaggaac caattgata ttatttcga atggaaaat 
      tccaacaac tgcctttca tgattgggt acacgactg 
      ccgaaactt ggggctttg actcgctca ggcctgacg 
      attggccag gggcgaaaa atctggaag actcaatcc 
      cctggactc taggattta aaactggaa gcccggaaa 
      gtcaagtca tgacggaga aaataaaaa agttctatt 
      gcggcggac atattccga ccaccatta aattagaga 
      caaaaattg cgatccaca ccgatgcaa atttcgttt 
      tcggggttt ataaaacgg ctctaagcc acttcagca 
      agtcacaag cccccactt atttgtaca ggttttcgg 
      cagagactg aactgaatg tgatgcggc aattctaca 
      agatcaaaa ttccaggtg tggtccatt gtgacgata 
      ttggttatc tgttcaaaa acgtggctt cgcctcgca 
      tcaaacccg gttctaagg aagaagttt tgttgaaaa 
      aggtcgtaa agttgtgga cgaattttg ccaaattcg 
      gcaatccca cacctgaca actccttgg gtgttgtct 
      ggaaacccg ttactgaca aagaagttg attatttgg 
      acattggcg agagtctga cgccggcta gaaactcga 
      ttgagcaca agagagtac aatttttga caaaactgt 
      acgagcgca agaactcct cgtcccatt tggtaaaaa 
      caaacaata aacaatatg atacaggaa gccaacaac 
      aacaacaac aacaacaac aacaacaac aacaacaac 
      aacaacaac ccactgcgt ttcaagtgt aatgcctct 
      tttgaactc atcactcat cttgtttgt tactcttat 
      acaaaatgt gttgtacgt gcaaaaacc gttagatgt 
      tgtagatat gtacatcgt cttcactag tgaaagtcc 
      taacaacac aagatggtc tcctctctg gaaaagttt 
      atttttcaa cgatgttga ttaccaatg gaagaagat 
      gaatctggg ttatttgag caatactgc aatagaaat 
      atttaaata tgaaaatgt ttctatagc aaactctgt 
      aatatattt taaattaag ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY