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Gene information
General information
TC015213 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1381025..1388679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015213
    iBeetle screen iB_05779

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015213 RA
      MGEPEALKY KEEGNAAFK SGNWDSAAK LYTKAINLE 
      TSESRDLSV FLKNRAAAY LKLGKFEEA LSDCDRSLE 
      IVPRDPKAL FRRCQALEA LERFEEAYR DATQIFKDD 
      PNNRTIQPV LERLYRIVQ ERVKENAQT STKIESMTK 
      LAFDLAADR EKRETAMNN LLVLARENA GSSLMIKSP 
      VVQQIKKLL KVEKNREIC VTGIRIIGE LCKHNVDKT 
      RSVMKDVGI PWFLEILDS NHEQQVNAA QYCIQQILN 
      IFSGMDNKT DSKPDQELV QKNKKDIDM ILTCLVYSI 
      NNQAISGLA RDAIIELLT RNIHYTALN WAEQLVEIG 
      GVSRLMECA SELEEYKYE SAMNITPST RTIAAVCLS 
      RIYENMYYD AAREKFMTK IQDFIKDKL LTPDIESKV 
      RVAVAITAL LRGPLDVGN AIIGKEGIM EMILVMANT 
      DDLLQQKVA CECIVAAAS KADKAKAII AQGVNILKT 
      LYKSKDDAI RVRALVGLC KLGSSGGTD AAKKPFAEG 
      SNLKLAEAC RRFLVHPGN DADIRKWAA EGLSYLTLD 
      AEVKEKLVE DRPALQSLI ELAKTGDQS CLFGVVTTL 
      VNLCNAYEK QEIIPEMVE LAKFAKQHI PEEHELDDP 
      DFVTKRILV LGKEGVTSA LVALSKTES PNSRELISR 
      VFNSLCSEQ ELRGVVVSQ GGSKALIPL ALKGTEKGK 
      RHAAQALAR IAITMNPEV AFPGQRALE VIRPLVGLL 
      HPDCTGLEN FEALMGLCN IAQMSESAR QRILKEGGL 
      SKIEHYMFE DHEYLCRAA TQCMTNMCM SPDVIKTHE 
      GANDKVKYF VSLSMDEDA DTAMAASGA LAILTGNSK 
      KCCEKIFDS KNWLDCFHV LLANPVASL QYRGVCIVR 
      NVIENSKEL AEKLMETDI MEILMALKL QTDENKQSV 
      VKVAEDALK LAETWGLIK KPELD
    CDS sequence
    • >TC015213 RA
      atgggcgaa cccgaagcg ttaaaatac aaggaagaa 
      ggcaacgcg gccttcaaa agtggcaat tgggactcg 
      gcggccaaa ctctacacc aaagccatc aacttggag 
      acgagcgag agtcgggac ttgtccgtg ttcctcaaa 
      aaccgggct gcggcctat ttgaagttg ggcaagttt 
      gaggaggcg ttgagcgac tgcgaccgc agtctggag 
      atagtgccc cgcgacccc aaggcgctg ttccgccgg 
      tgccaggcc ttggaggcg ctggaacgg tttgaggag 
      gcttatcgg gacgccacc cagattttc aaggatgac 
      cccaataat aggacgatt cagccggtt ttggagcga 
      ttgtatcgt attgtccag gaacgggtt aaggaaaat 
      gcccagacc agcactaag atcgagagt atgacgaag 
      ttggcgttt gatttggcc gccgatagg gagaaacgc 
      gagactgcg atgaataat ttgttggtg cttgcgagg 
      gaaaatgcg gggtcgagt cttatgatt aaatcgccg 
      gttgttcag cagattaag aagttgctt aaagtggag 
      aagaatcgg gaaatatgt gttactggt atacgaatt 
      atcggcgag ctttgtaag cataatgtg gataagaca 
      cgaagtgtt atgaaggat gtggggatc ccctggttt 
      ttggaaatt ttggactcg aatcatgaa cagcaagtt 
      aacgcagcg cagtattgc atccaacaa atcctcaat 
      attttctca ggaatggac aacaaaacc gacagcaaa 
      cctgaccaa gaacttgtg caaaaaaac aaaaaagac 
      atcgacatg atcctgaca tgtctcgtc tactccatt 
      aacaatcaa gcaatcagt ggcctggcc cgcgacgca 
      attatcgag ctcctcacc cgaaatatc cactatacg 
      gccctgaac tgggccgag caattggtc gaaatcgga 
      ggcgtctca agacttatg gagtgtgcc agcgaatta 
      gaagaatac aaatacgag agtgccatg aacatcaca 
      ccctcgacc cgtacaatc gccgccgtt tgtttatcc 
      cgaatctac gaaaacatg tactacgac gccgcccgc 
      gagaaattc atgacaaaa atccaggat tttatcaaa 
      gataagctc ctaacgccc gacattgag tcgaaagtt 
      agggtggct gtggcgata acagccctg ctgcggggg 
      ccgctggac gttggtaat gccattatc ggtaaagaa 
      gggatcatg gaaatgatc ctggtcatg gccaacacc 
      gatgacttg ctccaacag aaagttgcg tgcgagtgt 
      attgttgca gcggcaagt aaagccgac aaggccaag 
      gcgattata gcacaaggt gttaatatt ttgaagact 
      ttgtataag tccaaggat gatgcgata agggtgagg 
      gctttagtg gggctttgt aagttgggg agttcgggg 
      ggcactgac gcggcaaag aaacccttt gccgaaggg 
      tctaatcta aagttggcc gaagcctgc aggaggttt 
      ttggtgcat ccggggaac gatgcggat attaggaag 
      tgggctgct gaaggcctt tcctactta accctcgac 
      gccgaagtg aaagaaaaa ctagtggaa gaccgcccc 
      gccctccaa tccctcatc gaactcgca aaaaccggc 
      gaccaatcg tgccttttc ggcgtcgtc acaaccctc 
      gtgaattta tgcaacgct tacgaaaaa caagaaatc 
      atcccggaa atggtcgaa ctggcaaaa ttcgccaaa 
      caacacatc ccggaagag cacgaactc gacgaccct 
      gactttgta acaaaaaga atcctagtt ttgggcaaa 
      gagggcgtc acctccgcc cttgtggcc ctaagcaag 
      accgaaagc cccaattca cgcgaacta atctcgcgc 
      gtgttcaac tccctgtgc agcgaacag gaattgcgc 
      ggcgtggtg gtgtcccaa gggggctcc aaagccctc 
      atcccgctg gccttgaag ggcaccgaa aagggcaaa 
      cgacacgca gcgcaagct ttggcccgg atcgcgatc 
      acgatgaac cccgaagtg gccttcccg gggcagcgg 
      gccctcgag gtgatccgg cccttggta gggctcctg 
      caccccgac tgcacgggc ttggagaac tttgaggcc 
      ctgatgggg ctgtgcaac atcgcgcaa atgagcgag 
      agcgcgcgg cagaggatt ctgaaggaa ggggggctg 
      tctaagatt gagcattac atgttcgag gatcatgaa 
      tatttgtgc agggcggcc acacaatgc atgaccaac 
      atgtgcatg tcgcctgat gttataaag acacacgag 
      ggggccaat gataaggtc aagtatttc gtgtcttta 
      tcgatggat gaggacgct gatacggcc atggccgcc 
      tcaggagcc ctggcaatt ctcaccgga aatagcaaa 
      aaatgttgc gagaaaatt ttcgactcg aaaaattgg 
      ttggactgc ttccacgtg ttgttggcc aatccggtc 
      gctagtttg caataccgg ggcgtttgt atcgtgcga 
      aatgttatc gaaaattcc aaagaactg gccgaaaag 
      ttgatggag accgacatt atggaaatt ctaatggcg 
      ttaaaactg caaaccgat gagaacaaa caatcagtc 
      gtgaaagtg gcagaggac gcgctcaag ttggccgag 
      acttggggg ctcatcaag aaaccggaa ctagactag
    mRNA sequence
    • >TC015213 RA
      caaaactgt gaccaagtc gtgcgtgag cgccttaat 
      tgtctaatt actaataat tgatgcgat tattgcatt 
      aaaattagt ttatggcac cgcctctga acctaacct 
      caaacctgg caaatgtcc aaaaatagc cttttaaat 
      ttagaaaat tcctctcgc gtctcgaaa agcccagaa 
      acttcgact caaaccgta taaatacgg catgtggcc 
      acgctgtca caacctcaa gtgaaacag tgtgcacat 
      aaccccaaa atgggcgaa cccgaagcg ttaaaatac 
      aaggaagaa ggcaacgcg gccttcaaa agtggcaat 
      tgggactcg gcggccaaa ctctacacc aaagccatc 
      aacttggag acgagcgag agtcgggac ttgtccgtg 
      ttcctcaaa aaccgggct gcggcctat ttgaagttg 
      ggcaagttt gaggaggcg ttgagcgac tgcgaccgc 
      agtctggag atagtgccc cgcgacccc aaggcgctg 
      ttccgccgg tgccaggcc ttggaggcg ctggaacgg 
      tttgaggag gcttatcgg gacgccacc cagattttc 
      aaggatgac cccaataat aggacgatt cagccggtt 
      ttggagcga ttgtatcgt attgtccag gaacgggtt 
      aaggaaaat gcccagacc agcactaag atcgagagt 
      atgacgaag ttggcgttt gatttggcc gccgatagg 
      gagaaacgc gagactgcg atgaataat ttgttggtg 
      cttgcgagg gaaaatgcg gggtcgagt cttatgatt 
      aaatcgccg gttgttcag cagattaag aagttgctt 
      aaagtggag aagaatcgg gaaatatgt gttactggt 
      atacgaatt atcggcgag ctttgtaag cataatgtg 
      gataagaca cgaagtgtt atgaaggat gtggggatc 
      ccctggttt ttggaaatt ttggactcg aatcatgaa 
      cagcaagtt aacgcagcg cagtattgc atccaacaa 
      atcctcaat attttctca ggaatggac aacaaaacc 
      gacagcaaa cctgaccaa gaacttgtg caaaaaaac 
      aaaaaagac atcgacatg atcctgaca tgtctcgtc 
      tactccatt aacaatcaa gcaatcagt ggcctggcc 
      cgcgacgca attatcgag ctcctcacc cgaaatatc 
      cactatacg gccctgaac tgggccgag caattggtc 
      gaaatcgga ggcgtctca agacttatg gagtgtgcc 
      agcgaatta gaagaatac aaatacgag agtgccatg 
      aacatcaca ccctcgacc cgtacaatc gccgccgtt 
      tgtttatcc cgaatctac gaaaacatg tactacgac 
      gccgcccgc gagaaattc atgacaaaa atccaggat 
      tttatcaaa gataagctc ctaacgccc gacattgag 
      tcgaaagtt agggtggct gtggcgata acagccctg 
      ctgcggggg ccgctggac gttggtaat gccattatc 
      ggtaaagaa gggatcatg gaaatgatc ctggtcatg 
      gccaacacc gatgacttg ctccaacag aaagttgcg 
      tgcgagtgt attgttgca gcggcaagt aaagccgac 
      aaggccaag gcgattata gcacaaggt gttaatatt 
      ttgaagact ttgtataag tccaaggat gatgcgata 
      agggtgagg gctttagtg gggctttgt aagttgggg 
      agttcgggg ggcactgac gcggcaaag aaacccttt 
      gccgaaggg tctaatcta aagttggcc gaagcctgc 
      aggaggttt ttggtgcat ccggggaac gatgcggat 
      attaggaag tgggctgct gaaggcctt tcctactta 
      accctcgac gccgaagtg aaagaaaaa ctagtggaa 
      gaccgcccc gccctccaa tccctcatc gaactcgca 
      aaaaccggc gaccaatcg tgccttttc ggcgtcgtc 
      acaaccctc gtgaattta tgcaacgct tacgaaaaa 
      caagaaatc atcccggaa atggtcgaa ctggcaaaa 
      ttcgccaaa caacacatc ccggaagag cacgaactc 
      gacgaccct gactttgta acaaaaaga atcctagtt 
      ttgggcaaa gagggcgtc acctccgcc cttgtggcc 
      ctaagcaag accgaaagc cccaattca cgcgaacta 
      atctcgcgc gtgttcaac tccctgtgc agcgaacag 
      gaattgcgc ggcgtggtg gtgtcccaa gggggctcc 
      aaagccctc atcccgctg gccttgaag ggcaccgaa 
      aagggcaaa cgacacgca gcgcaagct ttggcccgg 
      atcgcgatc acgatgaac cccgaagtg gccttcccg 
      gggcagcgg gccctcgag gtgatccgg cccttggta 
      gggctcctg caccccgac tgcacgggc ttggagaac 
      tttgaggcc ctgatgggg ctgtgcaac atcgcgcaa 
      atgagcgag agcgcgcgg cagaggatt ctgaaggaa 
      ggggggctg tctaagatt gagcattac atgttcgag 
      gatcatgaa tatttgtgc agggcggcc acacaatgc 
      atgaccaac atgtgcatg tcgcctgat gttataaag 
      acacacgag ggggccaat gataaggtc aagtatttc 
      gtgtcttta tcgatggat gaggacgct gatacggcc 
      atggccgcc tcaggagcc ctggcaatt ctcaccgga 
      aatagcaaa aaatgttgc gagaaaatt ttcgactcg 
      aaaaattgg ttggactgc ttccacgtg ttgttggcc 
      aatccggtc gctagtttg caataccgg ggcgtttgt 
      atcgtgcga aatgttatc gaaaattcc aaagaactg 
      gccgaaaag ttgatggag accgacatt atggaaatt 
      ctaatggcg ttaaaactg caaaccgat gagaacaaa 
      caatcagtc gtgaaagtg gcagaggac gcgctcaag 
      ttggccgag acttggggg ctcatcaag aaaccggaa 
      ctagactag ccttttata cgaaaaatt ttgtatcgt 
      ttaaaataa taaattatt gttaattac ctcgcgtct 
      tgctctaat cctccgtgg catccttac ttcacccaa 
      aaacgcaca caacactag aatttggcc gagtttttc 
      aaattccta accacactt tttgtttga aagttggaa 
      gtggccaca gtcactttg ctaaaccaa acgaaataa 
      aaaattcac agtgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY