Status
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Gene information
General information
TC015209 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1469091..1479796
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015209
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05778

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015209 RA
      MLVKCPRVK RIYLKVFTA VFIIAILIL FLQHFNEKL 
      PTKPVSKLA RLYEKQIRD YERKIIPNL GHNGEAAFL 
      EGQDAKEGE KALKKFALN TVLSDRMPL DRKLRDPRN 
      PKCKTFTYN PKLKASVVV IFYNELLSV ILRTVWSVI 
      LQTPKELLE EIILVDDAS TEESLKGLL DYYIETRLS 
      STKLRLIHL KTRMGLIRA RLQGARIAT GDVLIFLDA 
      HCEATTDWM EPLLSRIEQ EPTAVLVPI IDVIEANTL 
      AYSTNGDTS YQVGGFSWS GHFTWIDIQ NEEDKHKLT 
      PVKSPTMAG GLFAIDRKF FWEIGSYDE QMDGWGGEN 
      LEMSFRIWQ CGGRLETVP CSRVGHIFR DFHPYSFPD 
      NKDTHGINT ARLAHVWMD DYKRFFFMY QPALENNPV 
      VGDLTHRKQ LRQKLRCKS FKWYLENVY PEKFIPDEN 
      VYAHGQVQN DYGMCLDDL QLGEDKIGP LGLYQCHPY 
      LAMSQYFSL NFKGELRKE NFCAETFGV REVQLTECH 
      GHKREQFWM FYKNGTIYN PTSGKCLSS AGVENGKGV 
      VVENCADSI FQKWSFTNV NRTALVV
    CDS sequence
    • >TC015209 RA
      atgttagta aaatgccca cgagttaaa cgcatctac 
      ttgaaagtt tttacggcg gtgtttatt atcgcaatt 
      ttgatactt tttttgcaa catttcaac gaaaaactc 
      ccgactaaa cctgtatca aagttggcc agactttac 
      gagaaacaa attagggat tatgagagg aagattatc 
      cccaatttg gggcacaac ggcgaggcg gcgtttctg 
      gagggccag gatgccaaa gagggagaa aaggctttg 
      aaaaaattc gcacttaat acggtccta agtgacagg 
      atgccgtta gataggaaa ttaagggac ccaagaaat 
      cctaaatgc aagactttt acatataac ccgaaactg 
      aaagcttct gttgttgtg atattttat aacgagctt 
      ttgagcgtt attttgagg accgtctgg agcgtgatt 
      ttacaaacg cccaaagag ttactggag gaaattatt 
      ctagtggac gatgcaagc actgaagaa agcctcaaa 
      ggccttctg gattattac atcgagacc cgcctctcc 
      tcgaccaaa ctccgcctc atccacctc aaaacccgc 
      atgggtttg attcgtgcc cgcctccaa ggggcgcgc 
      attgccaca ggggacgtc ctcattttc ctggacgcc 
      cactgtgag gcgaccact gattggatg gaacccctc 
      ctttcccgc attgaacaa gaaccaacc gccgtcctt 
      gtaccaatc atcgacgtg attgaagcc aacaccttg 
      gcttattca accaatggc gacacgtcg taccaagtg 
      ggcgggttt tcgtggtcc ggtcatttc acctggatt 
      gacatccag aacgaagag gacaaacat aaattgact 
      ccggtcaag tcaccaaca atggccggg ggtttgttt 
      gccatagac cggaagttt ttctgggaa attgggtct 
      tacgatgaa cagatggat ggctggggt ggggagaat 
      ctggaaatg agtttccgg atttggcag tgtggggga 
      cgtctggaa acggtgcct tgttccagg gttgggcac 
      attttccgg gattttcac ccgtattcc ttcccggat 
      aacaaggac acccatggg attaatacg gcgcgactt 
      gcccatgtg tggatggat gattataaa aggttcttt 
      tttatgtac cagccagct ttggagaat aatccagtg 
      gtcggggat ttgacacat cgcaaacaa ttgaggcaa 
      aaattgaga tgtaagagc ttcaagtgg tatttggaa 
      aatgtctat cccgaaaaa ttcatcccg gatgagaat 
      gtctacgcc catgggcag gtccagaat gattatggg 
      atgtgtctg gatgacctc cagctaggg gaggataaa 
      atcgggcct ttgggtctg taccagtgc cacccatat 
      ttggcaatg tcgcagtat ttctccctg aactttaaa 
      ggggagctg aggaaggag aatttctgt gccgaaacg 
      tttggcgta cgagaggta caattgacg gaatgtcat 
      ggtcataaa agggaacaa ttttggatg ttttataaa 
      aatggaaca atctataat cccacgtct ggcaaatgt 
      ctaagtagt gctggagtg gaaaatgga aaaggagtc 
      gttgtggaa aattgtgcc gatagtatt ttccagaaa 
      tggagtttt actaatgtt aatcgaacc gctcttgtg 
      gtgtaa
    mRNA sequence
    • >TC015209 RA
      gtcttaatg atgacaaaa catcacacg taatcgact 
      aattaggtt tttggaccg gatcgcact ttgggttgg 
      caacagtga tgtgaacgt caaccggtg acataacct 
      caaaatttc gtttttttt cagtcgagt ttttttgaa 
      ttttttgaa taaaaacac accaaaggc gcgtttttc 
      gttaatcag tgagttaca attaaccgg actgcgtaa 
      tttcgtgtt aattaatga caaaaaccg aatttagtc 
      aagtgccaa caagtcaat ttttttcct agataagta 
      gcacacact taattggga atatgcgtt gatagataa 
      gtgaaactt ggctcgaga gtgtccaaa taatgttag 
      taaaatgcc cacgagtta aacgcatct acttgaaag 
      tttttacgg cggtgttta ttatcgcaa ttttgatac 
      tttttttgc aacatttca acgaaaaac tcccgacta 
      aacctgtat caaagttgg ccagacttt acgagaaac 
      aaattaggg attatgaga ggaagatta tccccaatt 
      tggggcaca acggcgagg cggcgtttc tggagggcc 
      aggatgcca aagagggag aaaaggctt tgaaaaaat 
      tcgcactta atacggtcc taagtgaca ggatgccgt 
      tagatagga aattaaggg acccaagaa atcctaaat 
      gcaagactt ttacatata acccgaaac tgaaagctt 
      ctgttgttg tgatatttt ataacgagc ttttgagcg 
      ttattttga ggaccgtct ggagcgtga ttttacaaa 
      cgcccaaag agttactgg aggaaatta ttctagtgg 
      acgatgcaa gcactgaag aaagcctca aaggccttc 
      tggattatt acatcgaga cccgcctct cctcgacca 
      aactccgcc tcatccacc tcaaaaccc gcatgggtt 
      tgattcgtg cccgcctcc aaggggcgc gcattgcca 
      caggggacg tcctcattt tcctggacg cccactgtg 
      aggcgacca ctgattgga tggaacccc tcctttccc 
      gcattgaac aagaaccaa ccgccgtcc ttgtaccaa 
      tcatcgacg tgattgaag ccaacacct tggcttatt 
      caaccaatg gcgacacgt cgtaccaag tgggcgggt 
      tttcgtggt ccggtcatt tcacctgga ttgacatcc 
      agaacgaag aggacaaac ataaattga ctccggtca 
      agtcaccaa caatggccg ggggtttgt ttgccatag 
      accggaagt ttttctggg aaattgggt cttacgatg 
      aacagatgg atggctggg gtggggaga atctggaaa 
      tgagtttcc ggatttggc agtgtgggg gacgtctgg 
      aaacggtgc cttgttcca gggttgggc acattttcc 
      gggattttc acccgtatt ccttcccgg ataacaagg 
      acacccatg ggattaata cggcgcgac ttgcccatg 
      tgtggatgg atgattata aaaggttct tttttatgt 
      accagccag ctttggaga ataatccag tggtcgggg 
      atttgacac atcgcaaac aattgaggc aaaaattga 
      gatgtaaga gcttcaagt ggtatttgg aaaatgtct 
      atcccgaaa aattcatcc cggatgaga atgtctacg 
      cccatgggc aggtccaga atgattatg ggatgtgtc 
      tggatgacc tccagctag gggaggata aaatcgggc 
      ctttgggtc tgtaccagt gccacccat atttggcaa 
      tgtcgcagt atttctccc tgaacttta aaggggagc 
      tgaggaagg agaatttct gtgccgaaa cgtttggcg 
      tacgagagg tacaattga cggaatgtc atggtcata 
      aaagggaac aattttgga tgttttata aaaatggaa 
      caatctata atcccacgt ctggcaaat gtctaagta 
      gtgctggag tggaaaatg gaaaaggag tcgttgtgg 
      aaaattgtg ccgatagta ttttccaga aatggagtt 
      ttactaatg ttaatcgaa ccgctcttg tggtgtaat 
      taaatattg tgataattt agaagaacg ggtttagga 
      gacaggtca aggtctttg tatgtctat cacgtaagt 
      taaagtggt gctcttcct ataagtata aatgtacag 
      taatcgcct gtaactatt actcgttgg tttgtttaa 
      taaacaaat ttatttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY