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Gene information
General information
TC015207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1502328..1512466
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015207
    iBeetle screen iB_05776

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015207 RA
      MTRYTHADF TDDDSVNSV QLTEGISTS ATHIRYHAG 
TSIWKQRTM LEKALLVLI ATLFFVIVV LTIILHSAE 
HRIHESRII HVQSGDNLP CLNKSCVQI ASNILSAMD 
LSVDPCQDF YSYSCNGWV KANPIPDGK STWGTFMKL 
EQQNQLVIK HVLEQPIEN LKSKAEKKA KMYYESCLD 
VNDTVEDLG AEPMHALLK KLGGWSITD SGFNPKNWS 
FQHLVQMVQ NKYNVGALF SYMVGEDDR NSSRYILQI 
DQSGLTLPT RDYYLNESE HSKVLEAYL DYMTKVGVL 
LGGEVNSTK EQMQAVIDF ETRLANITT PNEMRRDEE 
SLYHLMTLA ELRQRAKFL DWRAFFENA MKIVGRKVN 
QKEKVVVYA PEYLENLSS LIKEYQSTP EGNITLNNY 
LVWQTVRVF TGCLSKAFR DAYKGLRKA LMGSEGGEE 
PQWRYCVQD TNNVLGFAI GAIFVREVF NLDSKTQAE 
EMINNVRNA FKANFAKLK WMDEDTRKA AVIKADAIS 
DMIGYPEFI RDPKQLDDR YDRLEVRSD TYFENNLNI 
NFYNLRKNL EKIREPVNK TTWSMAPST VNAYYTPTK 
NQMVFPAGI LQNPFYDPT FPPSLNYGG MGVVMGHEL 
THGFDDQGR EFDKDGNLN HWWKNKTIV LFKNRTKCV 
VDQYNKYTI NAKNVNGNQ TLGENIADN GGLKAAYHA 
YLTLMKDRP EPETLPGLT LNHKQLFFV AFAQVWCST 
VTKEAMSLQ IEKDSHSPA RFRVIGALS NLREFGEVF 
QCPAGSPMN PKNKCEVW
    CDS sequence
    • >TC015207 RA
      atgactcga tacacacac gccgacttc acagacgat 
gatagtgta aatagtgta cagttaacc gaaggtata 
agcaccagt gcgacccat atacggtac catgccggc 
accagcata tggaaacag cgcacgatg ctcgaaaag 
gcccttctg gtcctcata gcaacctta ttcttcgta 
attgtagtt ttaacaatc attctccat tcggcggag 
caccggata cacgaatcc aggataatt catgtgcaa 
tctggcgat aacctcccc tgcttgaac aaatcgtgc 
gtccaaatc gccagtaac attttatca gcgatggac 
ttgtctgtt gacccctgc caggatttt tactcctac 
tcttgcaat ggctgggtc aaagccaac ccgatcccc 
gacggtaaa agcacctgg ggcacgttt atgaagctg 
gagcagcaa aaccagctc gttattaag cacgtcctt 
gagcaaccg atcgaaaat ctcaaatcc aaggctgag 
aaaaaggcc aaaatgtac tacgagtcc tgtttggac 
gttaatgac actgttgag gacttgggg gcggagcca 
atgcacgcc ctcctcaaa aaactggga gggtggagt 
atcaccgac agtggtttt aacccgaaa aattggtcg 
ttccagcat ttggttcaa atggtgcaa aataaatat 
aatgttggg gcgttattt tcgtatatg gtgggggag 
gacgataga aattcctca aggtatata ctgcagatt 
gatcagagt gggctgacg ttgcccacg agggattat 
tacctgaat gagagcgaa cattcgaag gttttggag 
gcctacctt gattatatg accaaagtt ggggttttg 
ctagggggg gaggtgaac tcgaccaaa gaacaaatg 
caagctgtg atagatttt gagactaga ttagcaaat 
attacgaca ccgaatgag atgaggagg gatgaagag 
tcgctctat catttgatg acgttagct gagctcagg 
caaagggcc aagtttttg gattggcgg gcttttttc 
gaaaatgcg atgaaaatc gtggggcgg aaggtgaat 
cagaaggaa aaagtcgta gtgtacgcg cctgagtat 
ttagaaaat ttatcaagt ctgatcaaa gagtatcaa 
agtacgccc gagggcaat atcacttta aacaattat 
ctggtttgg caaaccgtt cgcgtgttt actggttgt 
ctctcgaag gcctttcga gacgcctac aagggcctg 
agaaaggct ctgatgggc tcagagggc ggtgaagag 
ccccaatgg cggtactgt gtccaagac acaaataat 
gttctgggt tttgcgatt ggtgctatt tttgttcgc 
gaagttttt aatctcgat tcgaagaca caagctgag 
gaaatgatc aataatgtg aggaacgcc ttcaaggct 
aattttgcc aaactcaag tggatggat gaggatacg 
aggaaggcg gctgtgatc aaggcggac gcgatttcg 
gacatgatc ggttacccc gagtttatc cgcgacccc 
aagcaactt gatgaccgt tacgaccgt ctggaggtc 
cgcagcgac acctacttt gagaacaac ctcaacatc 
aacttttac aatttacgc aaaaatttg gaaaaaatc 
cgcgaaccc gtcaataaa acaacctgg agtatggct 
ccttccacg gtcaacgcc tactacacc ccgaccaaa 
aaccagatg gtcttccct gccggcatc ctccagaac 
cccttctac gaccccacg ttccccccc agcctgaac 
tacgggggc atgggtgtc gtcatgggc cacgaactc 
acccatggc ttcgacgac caaggccgc gagtttgac 
aaagatggt aacttgaac cattggtgg aagaacaag 
accatcgtc ttgttcaaa aaccggaca aaatgcgtc 
gtggaccag tacaataaa tacaccatc aacgcgaaa 
aacgtcaac gggaatcaa actttgggc gaaaatatt 
gcagataac ggtggttta aaggcggca tatcacgcc 
tatctgact ctgatgaag gatcggccg gagcccgaa 
acgctccca gggctcact ttgaaccac aagcaactt 
ttttttgtg gcgtttgcc caagtttgg tgctcgacg 
gtcaccaag gaggctatg tccttgcaa attgagaaa 
gattcgcac tctccggcc aggtttagg gtcattggg 
gctctgagc aatttgagg gagtttggg gaggtgttc 
cagtgcccg gcggggtcg cccatgaac cccaaaaac 
aagtgcgag gtgtggtag
    mRNA sequence
    • >TC015207 RA
      taatcgagc aggccgtcg tctggcgtc ccaaatttt 
gaacgcagg gcgtcggcg attaaatca cgtcaccag 
tgccaaaaa aacccaagt gttgtgcaa aaccattcg 
gaaacgatg agcgtcaag gtgcgtaaa taatttatc 
ctaaagccg cattcacac gagcgcatt ccaagtcct 
aatcccttt tttgtatca gtggttgtg gtgggggct 
gtgacataa taatttaaa atggcgcag cgacgccat 
ctgacgtca caacatgtg ttgttaaat taagagacg 
cgccgacag tgttttgtt cggcgcggt ccaaggcga 
ctgcgattt attttcctt attggtttt atttcgggg 
aaataatat gactcgata cacacacgc cgacttcac 
agacgatga tagtgtaaa tagtgtaca gttaaccga 
aggtataag caccagtgc gacccatat acggtacca 
tgccggcac cagcatatg gaaacagcg cacgatgct 
cgaaaaggc ccttctggt cctcatagc aaccttatt 
cttcgtaat tgtagtttt aacaatcat tctccattc 
ggcggagca ccggataca cgaatccag gataattca 
tgtgcaatc tggcgataa cctcccctg cttgaacaa 
atcgtgcgt ccaaatcgc cagtaacat tttatcagc 
gatggactt gtctgttga cccctgcca ggattttta 
ctcctactc ttgcaatgg ctgggtcaa agccaaccc 
gatccccga cggtaaaag cacctgggg cacgtttat 
gaagctgga gcagcaaaa ccagctcgt tattaagca 
cgtccttga gcaaccgat cgaaaatct caaatccaa 
ggctgagaa aaaggccaa aatgtacta cgagtcctg 
tttggacgt taatgacac tgttgagga cttgggggc 
ggagccaat gcacgccct cctcaaaaa actgggagg 
gtggagtat caccgacag tggttttaa cccgaaaaa 
ttggtcgtt ccagcattt ggttcaaat ggtgcaaaa 
taaatataa tgttggggc gttattttc gtatatggt 
gggggagga cgatagaaa ttcctcaag gtatatact 
gcagattga tcagagtgg gctgacgtt gcccacgag 
ggattatta cctgaatga gagcgaaca ttcgaaggt 
tttggaggc ctaccttga ttatatgac caaagttgg 
ggttttgct aggggggga ggtgaactc gaccaaaga 
acaaatgca agctgtgat agattttga gactagatt 
agcaaatat tacgacacc gaatgagat gaggaggga 
tgaagagtc gctctatca tttgatgac gttagctga 
gctcaggca aagggccaa gtttttgga ttggcgggc 
ttttttcga aaatgcgat gaaaatcgt ggggcggaa 
ggtgaatca gaaggaaaa agtcgtagt gtacgcgcc 
tgagtattt agaaaattt atcaagtct gatcaaaga 
gtatcaaag tacgcccga gggcaatat cactttaaa 
caattatct ggtttggca aaccgttcg cgtgtttac 
tggttgtct ctcgaaggc ctttcgaga cgcctacaa 
gggcctgag aaaggctct gatgggctc agagggcgg 
tgaagagcc ccaatggcg gtactgtgt ccaagacac 
aaataatgt tctgggttt tgcgattgg tgctatttt 
tgttcgcga agtttttaa tctcgattc gaagacaca 
agctgagga aatgatcaa taatgtgag gaacgcctt 
caaggctaa ttttgccaa actcaagtg gatggatga 
ggatacgag gaaggcggc tgtgatcaa ggcggacgc 
gatttcgga catgatcgg ttaccccga gtttatccg 
cgaccccaa gcaacttga tgaccgtta cgaccgtct 
ggaggtccg cagcgacac ctactttga gaacaacct 
caacatcaa cttttacaa tttacgcaa aaatttgga 
aaaaatccg cgaacccgt caataaaac aacctggag 
tatggctcc ttccacggt caacgccta ctacacccc 
gaccaaaaa ccagatggt cttccctgc cggcatcct 
ccagaaccc cttctacga ccccacgtt cccccccag 
cctgaacta cgggggcat gggtgtcgt catgggcca 
cgaactcac ccatggctt cgacgacca aggccgcga 
gtttgacaa agatggtaa cttgaacca ttggtggaa 
gaacaagac catcgtctt gttcaaaaa ccggacaaa 
atgcgtcgt ggaccagta caataaata caccatcaa 
cgcgaaaaa cgtcaacgg gaatcaaac tttgggcga 
aaatattgc agataacgg tggtttaaa ggcggcata 
tcacgccta tctgactct gatgaagga tcggccgga 
gcccgaaac gctcccagg gctcacttt gaaccacaa 
gcaactttt ttttgtggc gtttgccca agtttggtg 
ctcgacggt caccaagga ggctatgtc cttgcaaat 
tgagaaaga ttcgcactc tccggccag gtttagggt 
cattggggc tctgagcaa tttgaggga gtttgggga 
ggtgttcca gtgcccggc ggggtcgcc catgaaccc 
caaaaacaa gtgcgaggt gtggtaggt tgtacctga 
tttttctaa ggtgagttg ggggcgttg gcaatttct 
ggattaatt aaatcaagt cctcagcaa aatttacga 
aaaaaatgg tcaaaaaaa tcatcaaaa attgggcaa 
acagtcccc aactcaccc tgtttcgta gctgaagtt 
tcagtttcc atatcaact tatttacat ggacgtgta 
tggttcttg tccttgttt gtgtaaatt tgtgtacct 
atattttta ctgtgataa cgagaataa acgtaaata 
tttaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY