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Gene information
General information
TC015207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1502328..1512466
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015207
    iBeetle screen iB_05776

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015207 RA
      MTRYTHADF TDDDSVNSV QLTEGISTS ATHIRYHAG 
      TSIWKQRTM LEKALLVLI ATLFFVIVV LTIILHSAE 
      HRIHESRII HVQSGDNLP CLNKSCVQI ASNILSAMD 
      LSVDPCQDF YSYSCNGWV KANPIPDGK STWGTFMKL 
      EQQNQLVIK HVLEQPIEN LKSKAEKKA KMYYESCLD 
      VNDTVEDLG AEPMHALLK KLGGWSITD SGFNPKNWS 
      FQHLVQMVQ NKYNVGALF SYMVGEDDR NSSRYILQI 
      DQSGLTLPT RDYYLNESE HSKVLEAYL DYMTKVGVL 
      LGGEVNSTK EQMQAVIDF ETRLANITT PNEMRRDEE 
      SLYHLMTLA ELRQRAKFL DWRAFFENA MKIVGRKVN 
      QKEKVVVYA PEYLENLSS LIKEYQSTP EGNITLNNY 
      LVWQTVRVF TGCLSKAFR DAYKGLRKA LMGSEGGEE 
      PQWRYCVQD TNNVLGFAI GAIFVREVF NLDSKTQAE 
      EMINNVRNA FKANFAKLK WMDEDTRKA AVIKADAIS 
      DMIGYPEFI RDPKQLDDR YDRLEVRSD TYFENNLNI 
      NFYNLRKNL EKIREPVNK TTWSMAPST VNAYYTPTK 
      NQMVFPAGI LQNPFYDPT FPPSLNYGG MGVVMGHEL 
      THGFDDQGR EFDKDGNLN HWWKNKTIV LFKNRTKCV 
      VDQYNKYTI NAKNVNGNQ TLGENIADN GGLKAAYHA 
      YLTLMKDRP EPETLPGLT LNHKQLFFV AFAQVWCST 
      VTKEAMSLQ IEKDSHSPA RFRVIGALS NLREFGEVF 
      QCPAGSPMN PKNKCEVW
    CDS sequence
    • >TC015207 RA
      atgactcga tacacacac gccgacttc acagacgat 
      gatagtgta aatagtgta cagttaacc gaaggtata 
      agcaccagt gcgacccat atacggtac catgccggc 
      accagcata tggaaacag cgcacgatg ctcgaaaag 
      gcccttctg gtcctcata gcaacctta ttcttcgta 
      attgtagtt ttaacaatc attctccat tcggcggag 
      caccggata cacgaatcc aggataatt catgtgcaa 
      tctggcgat aacctcccc tgcttgaac aaatcgtgc 
      gtccaaatc gccagtaac attttatca gcgatggac 
      ttgtctgtt gacccctgc caggatttt tactcctac 
      tcttgcaat ggctgggtc aaagccaac ccgatcccc 
      gacggtaaa agcacctgg ggcacgttt atgaagctg 
      gagcagcaa aaccagctc gttattaag cacgtcctt 
      gagcaaccg atcgaaaat ctcaaatcc aaggctgag 
      aaaaaggcc aaaatgtac tacgagtcc tgtttggac 
      gttaatgac actgttgag gacttgggg gcggagcca 
      atgcacgcc ctcctcaaa aaactggga gggtggagt 
      atcaccgac agtggtttt aacccgaaa aattggtcg 
      ttccagcat ttggttcaa atggtgcaa aataaatat 
      aatgttggg gcgttattt tcgtatatg gtgggggag 
      gacgataga aattcctca aggtatata ctgcagatt 
      gatcagagt gggctgacg ttgcccacg agggattat 
      tacctgaat gagagcgaa cattcgaag gttttggag 
      gcctacctt gattatatg accaaagtt ggggttttg 
      ctagggggg gaggtgaac tcgaccaaa gaacaaatg 
      caagctgtg atagatttt gagactaga ttagcaaat 
      attacgaca ccgaatgag atgaggagg gatgaagag 
      tcgctctat catttgatg acgttagct gagctcagg 
      caaagggcc aagtttttg gattggcgg gcttttttc 
      gaaaatgcg atgaaaatc gtggggcgg aaggtgaat 
      cagaaggaa aaagtcgta gtgtacgcg cctgagtat 
      ttagaaaat ttatcaagt ctgatcaaa gagtatcaa 
      agtacgccc gagggcaat atcacttta aacaattat 
      ctggtttgg caaaccgtt cgcgtgttt actggttgt 
      ctctcgaag gcctttcga gacgcctac aagggcctg 
      agaaaggct ctgatgggc tcagagggc ggtgaagag 
      ccccaatgg cggtactgt gtccaagac acaaataat 
      gttctgggt tttgcgatt ggtgctatt tttgttcgc 
      gaagttttt aatctcgat tcgaagaca caagctgag 
      gaaatgatc aataatgtg aggaacgcc ttcaaggct 
      aattttgcc aaactcaag tggatggat gaggatacg 
      aggaaggcg gctgtgatc aaggcggac gcgatttcg 
      gacatgatc ggttacccc gagtttatc cgcgacccc 
      aagcaactt gatgaccgt tacgaccgt ctggaggtc 
      cgcagcgac acctacttt gagaacaac ctcaacatc 
      aacttttac aatttacgc aaaaatttg gaaaaaatc 
      cgcgaaccc gtcaataaa acaacctgg agtatggct 
      ccttccacg gtcaacgcc tactacacc ccgaccaaa 
      aaccagatg gtcttccct gccggcatc ctccagaac 
      cccttctac gaccccacg ttccccccc agcctgaac 
      tacgggggc atgggtgtc gtcatgggc cacgaactc 
      acccatggc ttcgacgac caaggccgc gagtttgac 
      aaagatggt aacttgaac cattggtgg aagaacaag 
      accatcgtc ttgttcaaa aaccggaca aaatgcgtc 
      gtggaccag tacaataaa tacaccatc aacgcgaaa 
      aacgtcaac gggaatcaa actttgggc gaaaatatt 
      gcagataac ggtggttta aaggcggca tatcacgcc 
      tatctgact ctgatgaag gatcggccg gagcccgaa 
      acgctccca gggctcact ttgaaccac aagcaactt 
      ttttttgtg gcgtttgcc caagtttgg tgctcgacg 
      gtcaccaag gaggctatg tccttgcaa attgagaaa 
      gattcgcac tctccggcc aggtttagg gtcattggg 
      gctctgagc aatttgagg gagtttggg gaggtgttc 
      cagtgcccg gcggggtcg cccatgaac cccaaaaac 
      aagtgcgag gtgtggtag
    mRNA sequence
    • >TC015207 RA
      taatcgagc aggccgtcg tctggcgtc ccaaatttt 
      gaacgcagg gcgtcggcg attaaatca cgtcaccag 
      tgccaaaaa aacccaagt gttgtgcaa aaccattcg 
      gaaacgatg agcgtcaag gtgcgtaaa taatttatc 
      ctaaagccg cattcacac gagcgcatt ccaagtcct 
      aatcccttt tttgtatca gtggttgtg gtgggggct 
      gtgacataa taatttaaa atggcgcag cgacgccat 
      ctgacgtca caacatgtg ttgttaaat taagagacg 
      cgccgacag tgttttgtt cggcgcggt ccaaggcga 
      ctgcgattt attttcctt attggtttt atttcgggg 
      aaataatat gactcgata cacacacgc cgacttcac 
      agacgatga tagtgtaaa tagtgtaca gttaaccga 
      aggtataag caccagtgc gacccatat acggtacca 
      tgccggcac cagcatatg gaaacagcg cacgatgct 
      cgaaaaggc ccttctggt cctcatagc aaccttatt 
      cttcgtaat tgtagtttt aacaatcat tctccattc 
      ggcggagca ccggataca cgaatccag gataattca 
      tgtgcaatc tggcgataa cctcccctg cttgaacaa 
      atcgtgcgt ccaaatcgc cagtaacat tttatcagc 
      gatggactt gtctgttga cccctgcca ggattttta 
      ctcctactc ttgcaatgg ctgggtcaa agccaaccc 
      gatccccga cggtaaaag cacctgggg cacgtttat 
      gaagctgga gcagcaaaa ccagctcgt tattaagca 
      cgtccttga gcaaccgat cgaaaatct caaatccaa 
      ggctgagaa aaaggccaa aatgtacta cgagtcctg 
      tttggacgt taatgacac tgttgagga cttgggggc 
      ggagccaat gcacgccct cctcaaaaa actgggagg 
      gtggagtat caccgacag tggttttaa cccgaaaaa 
      ttggtcgtt ccagcattt ggttcaaat ggtgcaaaa 
      taaatataa tgttggggc gttattttc gtatatggt 
      gggggagga cgatagaaa ttcctcaag gtatatact 
      gcagattga tcagagtgg gctgacgtt gcccacgag 
      ggattatta cctgaatga gagcgaaca ttcgaaggt 
      tttggaggc ctaccttga ttatatgac caaagttgg 
      ggttttgct aggggggga ggtgaactc gaccaaaga 
      acaaatgca agctgtgat agattttga gactagatt 
      agcaaatat tacgacacc gaatgagat gaggaggga 
      tgaagagtc gctctatca tttgatgac gttagctga 
      gctcaggca aagggccaa gtttttgga ttggcgggc 
      ttttttcga aaatgcgat gaaaatcgt ggggcggaa 
      ggtgaatca gaaggaaaa agtcgtagt gtacgcgcc 
      tgagtattt agaaaattt atcaagtct gatcaaaga 
      gtatcaaag tacgcccga gggcaatat cactttaaa 
      caattatct ggtttggca aaccgttcg cgtgtttac 
      tggttgtct ctcgaaggc ctttcgaga cgcctacaa 
      gggcctgag aaaggctct gatgggctc agagggcgg 
      tgaagagcc ccaatggcg gtactgtgt ccaagacac 
      aaataatgt tctgggttt tgcgattgg tgctatttt 
      tgttcgcga agtttttaa tctcgattc gaagacaca 
      agctgagga aatgatcaa taatgtgag gaacgcctt 
      caaggctaa ttttgccaa actcaagtg gatggatga 
      ggatacgag gaaggcggc tgtgatcaa ggcggacgc 
      gatttcgga catgatcgg ttaccccga gtttatccg 
      cgaccccaa gcaacttga tgaccgtta cgaccgtct 
      ggaggtccg cagcgacac ctactttga gaacaacct 
      caacatcaa cttttacaa tttacgcaa aaatttgga 
      aaaaatccg cgaacccgt caataaaac aacctggag 
      tatggctcc ttccacggt caacgccta ctacacccc 
      gaccaaaaa ccagatggt cttccctgc cggcatcct 
      ccagaaccc cttctacga ccccacgtt cccccccag 
      cctgaacta cgggggcat gggtgtcgt catgggcca 
      cgaactcac ccatggctt cgacgacca aggccgcga 
      gtttgacaa agatggtaa cttgaacca ttggtggaa 
      gaacaagac catcgtctt gttcaaaaa ccggacaaa 
      atgcgtcgt ggaccagta caataaata caccatcaa 
      cgcgaaaaa cgtcaacgg gaatcaaac tttgggcga 
      aaatattgc agataacgg tggtttaaa ggcggcata 
      tcacgccta tctgactct gatgaagga tcggccgga 
      gcccgaaac gctcccagg gctcacttt gaaccacaa 
      gcaactttt ttttgtggc gtttgccca agtttggtg 
      ctcgacggt caccaagga ggctatgtc cttgcaaat 
      tgagaaaga ttcgcactc tccggccag gtttagggt 
      cattggggc tctgagcaa tttgaggga gtttgggga 
      ggtgttcca gtgcccggc ggggtcgcc catgaaccc 
      caaaaacaa gtgcgaggt gtggtaggt tgtacctga 
      tttttctaa ggtgagttg ggggcgttg gcaatttct 
      ggattaatt aaatcaagt cctcagcaa aatttacga 
      aaaaaatgg tcaaaaaaa tcatcaaaa attgggcaa 
      acagtcccc aactcaccc tgtttcgta gctgaagtt 
      tcagtttcc atatcaact tatttacat ggacgtgta 
      tggttcttg tccttgttt gtgtaaatt tgtgtacct 
      atattttta ctgtgataa cgagaataa acgtaaata 
      tttaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY