| General information | |||
| TC015207 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 1502328..1512466 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015207 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05776 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015207 RA
MTRYTHADF TDDDSVNSV QLTEGISTS ATHIRYHAG TSIWKQRTM LEKALLVLI ATLFFVIVV LTIILHSAE HRIHESRII HVQSGDNLP CLNKSCVQI ASNILSAMD LSVDPCQDF YSYSCNGWV KANPIPDGK STWGTFMKL EQQNQLVIK HVLEQPIEN LKSKAEKKA KMYYESCLD VNDTVEDLG AEPMHALLK KLGGWSITD SGFNPKNWS FQHLVQMVQ NKYNVGALF SYMVGEDDR NSSRYILQI DQSGLTLPT RDYYLNESE HSKVLEAYL DYMTKVGVL LGGEVNSTK EQMQAVIDF ETRLANITT PNEMRRDEE SLYHLMTLA ELRQRAKFL DWRAFFENA MKIVGRKVN QKEKVVVYA PEYLENLSS LIKEYQSTP EGNITLNNY LVWQTVRVF TGCLSKAFR DAYKGLRKA LMGSEGGEE PQWRYCVQD TNNVLGFAI GAIFVREVF NLDSKTQAE EMINNVRNA FKANFAKLK WMDEDTRKA AVIKADAIS DMIGYPEFI RDPKQLDDR YDRLEVRSD TYFENNLNI NFYNLRKNL EKIREPVNK TTWSMAPST VNAYYTPTK NQMVFPAGI LQNPFYDPT FPPSLNYGG MGVVMGHEL THGFDDQGR EFDKDGNLN HWWKNKTIV LFKNRTKCV VDQYNKYTI NAKNVNGNQ TLGENIADN GGLKAAYHA YLTLMKDRP EPETLPGLT LNHKQLFFV AFAQVWCST VTKEAMSLQ IEKDSHSPA RFRVIGALS NLREFGEVF QCPAGSPMN PKNKCEVW
- >TC015207 RA
atgactcga tacacacac gccgacttc acagacgat gatagtgta aatagtgta cagttaacc gaaggtata agcaccagt gcgacccat atacggtac catgccggc accagcata tggaaacag cgcacgatg ctcgaaaag gcccttctg gtcctcata gcaacctta ttcttcgta attgtagtt ttaacaatc attctccat tcggcggag caccggata cacgaatcc aggataatt catgtgcaa tctggcgat aacctcccc tgcttgaac aaatcgtgc gtccaaatc gccagtaac attttatca gcgatggac ttgtctgtt gacccctgc caggatttt tactcctac tcttgcaat ggctgggtc aaagccaac ccgatcccc gacggtaaa agcacctgg ggcacgttt atgaagctg gagcagcaa aaccagctc gttattaag cacgtcctt gagcaaccg atcgaaaat ctcaaatcc aaggctgag aaaaaggcc aaaatgtac tacgagtcc tgtttggac gttaatgac actgttgag gacttgggg gcggagcca atgcacgcc ctcctcaaa aaactggga gggtggagt atcaccgac agtggtttt aacccgaaa aattggtcg ttccagcat ttggttcaa atggtgcaa aataaatat aatgttggg gcgttattt tcgtatatg gtgggggag gacgataga aattcctca aggtatata ctgcagatt gatcagagt gggctgacg ttgcccacg agggattat tacctgaat gagagcgaa cattcgaag gttttggag gcctacctt gattatatg accaaagtt ggggttttg ctagggggg gaggtgaac tcgaccaaa gaacaaatg caagctgtg atagatttt gagactaga ttagcaaat attacgaca ccgaatgag atgaggagg gatgaagag tcgctctat catttgatg acgttagct gagctcagg caaagggcc aagtttttg gattggcgg gcttttttc gaaaatgcg atgaaaatc gtggggcgg aaggtgaat cagaaggaa aaagtcgta gtgtacgcg cctgagtat ttagaaaat ttatcaagt ctgatcaaa gagtatcaa agtacgccc gagggcaat atcacttta aacaattat ctggtttgg caaaccgtt cgcgtgttt actggttgt ctctcgaag gcctttcga gacgcctac aagggcctg agaaaggct ctgatgggc tcagagggc ggtgaagag ccccaatgg cggtactgt gtccaagac acaaataat gttctgggt tttgcgatt ggtgctatt tttgttcgc gaagttttt aatctcgat tcgaagaca caagctgag gaaatgatc aataatgtg aggaacgcc ttcaaggct aattttgcc aaactcaag tggatggat gaggatacg aggaaggcg gctgtgatc aaggcggac gcgatttcg gacatgatc ggttacccc gagtttatc cgcgacccc aagcaactt gatgaccgt tacgaccgt ctggaggtc cgcagcgac acctacttt gagaacaac ctcaacatc aacttttac aatttacgc aaaaatttg gaaaaaatc cgcgaaccc gtcaataaa acaacctgg agtatggct ccttccacg gtcaacgcc tactacacc ccgaccaaa aaccagatg gtcttccct gccggcatc ctccagaac cccttctac gaccccacg ttccccccc agcctgaac tacgggggc atgggtgtc gtcatgggc cacgaactc acccatggc ttcgacgac caaggccgc gagtttgac aaagatggt aacttgaac cattggtgg aagaacaag accatcgtc ttgttcaaa aaccggaca aaatgcgtc gtggaccag tacaataaa tacaccatc aacgcgaaa aacgtcaac gggaatcaa actttgggc gaaaatatt gcagataac ggtggttta aaggcggca tatcacgcc tatctgact ctgatgaag gatcggccg gagcccgaa acgctccca gggctcact ttgaaccac aagcaactt ttttttgtg gcgtttgcc caagtttgg tgctcgacg gtcaccaag gaggctatg tccttgcaa attgagaaa gattcgcac tctccggcc aggtttagg gtcattggg gctctgagc aatttgagg gagtttggg gaggtgttc cagtgcccg gcggggtcg cccatgaac cccaaaaac aagtgcgag gtgtggtag
- >TC015207 RA
taatcgagc aggccgtcg tctggcgtc ccaaatttt gaacgcagg gcgtcggcg attaaatca cgtcaccag tgccaaaaa aacccaagt gttgtgcaa aaccattcg gaaacgatg agcgtcaag gtgcgtaaa taatttatc ctaaagccg cattcacac gagcgcatt ccaagtcct aatcccttt tttgtatca gtggttgtg gtgggggct gtgacataa taatttaaa atggcgcag cgacgccat ctgacgtca caacatgtg ttgttaaat taagagacg cgccgacag tgttttgtt cggcgcggt ccaaggcga ctgcgattt attttcctt attggtttt atttcgggg aaataatat gactcgata cacacacgc cgacttcac agacgatga tagtgtaaa tagtgtaca gttaaccga aggtataag caccagtgc gacccatat acggtacca tgccggcac cagcatatg gaaacagcg cacgatgct cgaaaaggc ccttctggt cctcatagc aaccttatt cttcgtaat tgtagtttt aacaatcat tctccattc ggcggagca ccggataca cgaatccag gataattca tgtgcaatc tggcgataa cctcccctg cttgaacaa atcgtgcgt ccaaatcgc cagtaacat tttatcagc gatggactt gtctgttga cccctgcca ggattttta ctcctactc ttgcaatgg ctgggtcaa agccaaccc gatccccga cggtaaaag cacctgggg cacgtttat gaagctgga gcagcaaaa ccagctcgt tattaagca cgtccttga gcaaccgat cgaaaatct caaatccaa ggctgagaa aaaggccaa aatgtacta cgagtcctg tttggacgt taatgacac tgttgagga cttgggggc ggagccaat gcacgccct cctcaaaaa actgggagg gtggagtat caccgacag tggttttaa cccgaaaaa ttggtcgtt ccagcattt ggttcaaat ggtgcaaaa taaatataa tgttggggc gttattttc gtatatggt gggggagga cgatagaaa ttcctcaag gtatatact gcagattga tcagagtgg gctgacgtt gcccacgag ggattatta cctgaatga gagcgaaca ttcgaaggt tttggaggc ctaccttga ttatatgac caaagttgg ggttttgct aggggggga ggtgaactc gaccaaaga acaaatgca agctgtgat agattttga gactagatt agcaaatat tacgacacc gaatgagat gaggaggga tgaagagtc gctctatca tttgatgac gttagctga gctcaggca aagggccaa gtttttgga ttggcgggc ttttttcga aaatgcgat gaaaatcgt ggggcggaa ggtgaatca gaaggaaaa agtcgtagt gtacgcgcc tgagtattt agaaaattt atcaagtct gatcaaaga gtatcaaag tacgcccga gggcaatat cactttaaa caattatct ggtttggca aaccgttcg cgtgtttac tggttgtct ctcgaaggc ctttcgaga cgcctacaa gggcctgag aaaggctct gatgggctc agagggcgg tgaagagcc ccaatggcg gtactgtgt ccaagacac aaataatgt tctgggttt tgcgattgg tgctatttt tgttcgcga agtttttaa tctcgattc gaagacaca agctgagga aatgatcaa taatgtgag gaacgcctt caaggctaa ttttgccaa actcaagtg gatggatga ggatacgag gaaggcggc tgtgatcaa ggcggacgc gatttcgga catgatcgg ttaccccga gtttatccg cgaccccaa gcaacttga tgaccgtta cgaccgtct ggaggtccg cagcgacac ctactttga gaacaacct caacatcaa cttttacaa tttacgcaa aaatttgga aaaaatccg cgaacccgt caataaaac aacctggag tatggctcc ttccacggt caacgccta ctacacccc gaccaaaaa ccagatggt cttccctgc cggcatcct ccagaaccc cttctacga ccccacgtt cccccccag cctgaacta cgggggcat gggtgtcgt catgggcca cgaactcac ccatggctt cgacgacca aggccgcga gtttgacaa agatggtaa cttgaacca ttggtggaa gaacaagac catcgtctt gttcaaaaa ccggacaaa atgcgtcgt ggaccagta caataaata caccatcaa cgcgaaaaa cgtcaacgg gaatcaaac tttgggcga aaatattgc agataacgg tggtttaaa ggcggcata tcacgccta tctgactct gatgaagga tcggccgga gcccgaaac gctcccagg gctcacttt gaaccacaa gcaactttt ttttgtggc gtttgccca agtttggtg ctcgacggt caccaagga ggctatgtc cttgcaaat tgagaaaga ttcgcactc tccggccag gtttagggt cattggggc tctgagcaa tttgaggga gtttgggga ggtgttcca gtgcccggc ggggtcgcc catgaaccc caaaaacaa gtgcgaggt gtggtaggt tgtacctga tttttctaa ggtgagttg ggggcgttg gcaatttct ggattaatt aaatcaagt cctcagcaa aatttacga aaaaaatgg tcaaaaaaa tcatcaaaa attgggcaa acagtcccc aactcaccc tgtttcgta gctgaagtt tcagtttcc atatcaact tatttacat ggacgtgta tggttcttg tccttgttt gtgtaaatt tgtgtacct atattttta ctgtgataa cgagaataa acgtaaata tttaga
