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Gene information
General information
TC015204 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1545351..1554407
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015204
    iBeetle screen iB_05774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015204 RA
      MSEPSVNDT SETESLEDE IEPKLKYVR LSNDLQNIL 
LTSSATCIG VHPKFVCLG SHWGVIHLL DHQGNSIKS 
KELRPHTVA VNQICIDGS GEYIATCSD DGKVFIHGL 
FSKENSLHL NVGRLVKTV ALDPLYYKN GANRRFITG 
DDKLTLYER TFLGSLKPT VLCESEGLV RSLCWGENF 
IAWSSNIGV RVYDITARC SLGLIKWEE HPGISIDKF 
RCNLRWADS RTLLIGWVD TVRVCVIRK RSNFEMANR 
DLPEFLVDP VSTFQTEFY ICGIAPLDH QLVLLGFPK 
ELDENQKSL RPQLYIVEY RDNDYSDVC TDSLSLRGY 
EEYTVNDYH LDVLIEENR FFIVAPRDV VIASPYDLD 
DRIQWFIQH NKFEEALDI LMQSDSRHI HRHTVQSVG 
IDYLDHLLS REMYDEAGK LCMKIFGKD SNTWEDQIY 
KFATVHQLR SVSPYIPRT LDSKLNPHI YEMILYEFL 
KLDAQGFLN LVKEWNPSL YNVSAVINA VLEHLLVCE 
VDKNLYLEA LAILYSFLK RYDKSLSMY LKLKHKDVF 
TLIQKHNLY GVIQDMLID LMELDHKKT IALLLEKNS 
ISSDKIVEK LKPTELHLY RFLDEYDKK NPKGKYHRE 
LVRLYAIFA REKLLPFLK KSDHYPIQE ALDICKKER 
FYPEMVYLL GRMGNIEEA LDLIINELK DMQQAISFC 
QEHDDPDLW NDLINHSLD KPEFIKFLL QSIGTYVDP 
TVLVQKIRN GMEVPGLKN SLVKMLYQY NLQVSVQEG 
CKKILVSDY FNLLRKLVK TQQKGIGVS EELMCGACH 
HMVLEKDPS RCTNITVFN CKHAFHEQC LPENTTRCG 
ICYPSDK
    CDS sequence
    • >TC015204 RA
      atgtctgag cccagcgtt aacgacacc agcgagact 
gagagcctt gaggacgaa atcgagcca aaactcaaa 
tacgtgcgc ttatctaac gacttgcag aacatcctg 
ttgacgtcc tcggccacc tgcatcggc gtgcacccc 
aagttcgtg tgtctgggc tcccattgg ggcgtgatc 
cacctgctg gaccaccaa ggcaacagt atcaagagc 
aaggagctg cgcccccat acggtggcc gttaaccag 
atttgcatc gacggcagt ggcgagtac atcgcgacg 
tgttcggat gatggcaag gttttcatc catggactt 
ttttcaaag gagaatagt ttgcatttg aatgtgggc 
agattggtg aagacggtg gctttggac ccgctgtat 
tacaaaaat ggggccaat cggcggttt atcacaggg 
gatgataag ttgacgctg tatgagcgc acgtttttg 
gggagtctt aaaccgact gtcttgtgc gagagcgag 
gggcttgtg aggagtctt tgttggggg gagaatttt 
attgcctgg tcgagtaat ataggggtg agggtgtat 
gatatcacg gcgaggtgc tcgctgggg ttgatcaag 
tgggaggag cacccggga atttcaatc gataaattt 
cggtgcaat ttacgatgg gccgatagt cggacccta 
ttaattggg tgggtggac acggtacga gtttgtgtg 
atacggaaa cgaagcaat tttgaaatg gccaatcgg 
gacttaccc gaattttta gtcgaccct gtttcaact 
ttccaaacc gaattttac atttgtgga attgcgccg 
ctcgaccac caattagtt ttactcggt ttcccgaaa 
gaattagac gaaaatcaa aaatcgtta cgtccccaa 
ctgtacata gtcgaatat cgggacaac gactattcc 
gatgtttgc accgatagt ttatcactt cgtggttac 
gaagaatac acagttaac gattaccac ttggatgtt 
ttaattgaa gaaaatcgc ttctttatt gttgcgcca 
cgagatgtc gttatagca agtccctat gatctagac 
gacaggatc cagtggttc atccaacac aataaattt 
gaggaggct ttggatatt ttaatgcaa agtgacagc 
cgccatatt caccgacac accgttcag tcggttggc 
attgactat ctagatcac ttattaagt cgggaaatg 
tacgacgaa gctggaaaa ttgtgcatg aaaatcttc 
ggtaaggac tcaaacacg tgggaagac caaatttat 
aagtttgca accgtgcat caattacga tctgtcagt 
ccctacatt ccacgcacg cttgattcg aaactaaac 
ccgcatatt tacgaaatg attttgtac gaatttttg 
aaactagat gcgcaaggt tttctgaac ttggttaag 
gaatggaac ccgagtttg tacaacgtt tcggctgtg 
attaacgcc gtcttggag catcttcta gtgtgtgaa 
gttgataag aatctatat ttggaagct ttggcgatt 
ttgtacagt tttttgaag cgttacgac aaaagcttg 
tccatgtat ttgaagtta aagcacaaa gacgtgttc 
acactgatc caaaaacac aatttgtac ggtgtgatc 
caggacatg ctcattgac cttatggag cttgaccat 
aagaaaact attgcactt ttattagag aaaaactca 
atttcgagc gataaaatc gtagagaag ctcaaaccg 
actgaatta catttatac aggttttta gacgagtat 
gataagaaa aatcctaag gggaagtac caccgtgag 
ttggtacgc ctgtatgcc attttcgcg cgcgaaaag 
ttgctcccg tttttgaaa aaatccgac cattatcca 
atccaggaa gctctggat atttgcaag aaggagagg 
ttttatccc gaaatggtt tatttgttg ggacggatg 
ggcaacatc gaagaagcc cttgacttg ataataaac 
gagttgaaa gacatgcaa caggcgatt tcgttttgt 
caggaacat gacgatccc gatttgtgg aatgacttg 
attaatcat tcgttggac aaacctgaa tttattaaa 
tttctcttg caaagtatc ggaacgtat gtggatccg 
accgtttta gtacaaaaa attaggaat gggatggaa 
gtgcccggt ttaaaaaat tcactagtt aaaatgttg 
tatcagtat aatttacaa gtgtctgta caggaaggg 
tgtaagaaa attctagtc tcggattat tttaatcta 
ttacggaag ctcgtcaag acgcaacag aaaggtatt 
ggcgtgtcg gaagaacta atgtgtggc gcttgtcac 
cacatggtg ctcgaaaag gacccatcc aggtgtacc 
aacataaca gttttcaat tgcaagcac gccttccac 
gaacaatgc ctccctgaa aacaccact cgatgcggc 
atctgctat ccatccgat aaataa
    mRNA sequence
    • >TC015204 RA
      tttattaaa catttaagt gcgtaaagg tccaatatt 
accggatat tgaggtatt aatgggcga atttgtgcg 
tggttggcg accgtgcct gtcaaatca ataatgaat 
cattagcac aaaatgcaa caattccac aaaaacaac 
aacaatgtc tgagcccag cgttaacga caccagcga 
gactgagag ccttgagga cgaaatcga gccaaaact 
caaatacgt gcgcttatc taacgactt gcagaacat 
cctgttgac gtcctcggc cacctgcat cggcgtgca 
ccccaagtt cgtgtgtct gggctccca ttggggcgt 
gatccacct gctggacca ccaaggcaa cagtatcaa 
gagcaagga gctgcgccc ccatacggt ggccgttaa 
ccagatttg catcgacgg cagtggcga gtacatcgc 
gacgtgttc ggatgatgg caaggtttt catccatgg 
acttttttc aaaggagaa tagtttgca tttgaatgt 
gggcagatt ggtgaagac ggtggcttt ggacccgct 
gtattacaa aaatggggc caatcggcg gtttatcac 
aggggatga taagttgac gctgtatga gcgcacgtt 
tttggggag tcttaaacc gactgtctt gtgcgagag 
cgaggggct tgtgaggag tctttgttg gggggagaa 
ttttattgc ctggtcgag taatatagg ggtgagggt 
gtatgatat cacggcgag gtgctcgct ggggttgat 
caagtggga ggagcaccc gggaatttc aatcgataa 
atttcggtg caatttacg atgggccga tagtcggac 
cctattaat tgggtgggt ggacacggt acgagtttg 
tgtgatacg gaaacgaag caattttga aatggccaa 
tcgggactt acccgaatt tttagtcga ccctgtttc 
aactttcca aaccgaatt ttacatttg tggaattgc 
gccgctcga ccaccaatt agttttact cggtttccc 
gaaagaatt agacgaaaa tcaaaaatc gttacgtcc 
ccaactgta catagtcga atatcggga caacgacta 
ttccgatgt ttgcaccga tagtttatc acttcgtgg 
ttacgaaga atacacagt taacgatta ccacttgga 
tgttttaat tgaagaaaa tcgcttctt tattgttgc 
gccacgaga tgtcgttat agcaagtcc ctatgatct 
agacgacag gatccagtg gttcatcca acacaataa 
atttgagga ggctttgga tattttaat gcaaagtga 
cagccgcca tattcaccg acacaccgt tcagtcggt 
tggcattga ctatctaga tcacttatt aagtcggga 
aatgtacga cgaagctgg aaaattgtg catgaaaat 
cttcggtaa ggactcaaa cacgtggga agaccaaat 
ttataagtt tgcaaccgt gcatcaatt acgatctgt 
cagtcccta cattccacg cacgcttga ttcgaaact 
aaacccgca tatttacga aatgatttt gtacgaatt 
tttgaaact agatgcgca aggttttct gaacttggt 
taaggaatg gaacccgag tttgtacaa cgtttcggc 
tgtgattaa cgccgtctt ggagcatct tctagtgtg 
tgaagttga taagaatct atatttgga agctttggc 
gattttgta cagtttttt gaagcgtta cgacaaaag 
cttgtccat gtatttgaa gttaaagca caaagacgt 
gttcacact gatccaaaa acacaattt gtacggtgt 
gatccagga catgctcat tgaccttat ggagcttga 
ccataagaa aactattgc acttttatt agagaaaaa 
ctcaatttc gagcgataa aatcgtaga gaagctcaa 
accgactga attacattt atacaggtt tttagacga 
gtatgataa gaaaaatcc taaggggaa gtaccaccg 
tgagttggt acgcctgta tgccatttt cgcgcgcga 
aaagttgct cccgttttt gaaaaaatc cgaccatta 
tccaatcca ggaagctct ggatatttg caagaagga 
gaggtttta tcccgaaat ggtttattt gttgggacg 
gatgggcaa catcgaaga agcccttga cttgataat 
aaacgagtt gaaagacat gcaacaggc gatttcgtt 
ttgtcagga acatgacga tcccgattt gtggaatga 
cttgattaa tcattcgtt ggacaaacc tgaatttat 
taaatttct cttgcaaag tatcggaac gtatgtgga 
tccgaccgt tttagtaca aaaaattag gaatgggat 
ggaagtgcc cggtttaaa aaattcact agttaaaat 
gttgtatca gtataattt acaagtgtc tgtacagga 
agggtgtaa gaaaattct agtctcgga ttattttaa 
tctattacg gaagctcgt caagacgca acagaaagg 
tattggcgt gtcggaaga actaatgtg tggcgcttg 
tcaccacat ggtgctcga aaaggaccc atccaggtg 
taccaacat aacagtttt caattgcaa gcacgcctt 
ccacgaaca atgcctccc tgaaaacac cactcgatg 
cggcatctg ctatccatc cgataaata aaacaacgg 
taagccaac aaataacta aatatattt ccaaaaatc 
gtaatcttc atcatcgtc cacatcgaa cggtccttc 
gaaatattc actctgtgg caaaagggc cggagtaaa 
agccaggaa atacttggc tgtgaattg gaaaacgtc 
ctctggttt taattggag gattgtttg gacgtaatc 
tgagatcat ttcacgcac ttctggctg gtcgtctaa 
ataattagc gatttgttt tttactcat catctgaaa 
catttttgt gtttgtgtc ttttttttt caccctaac 
tttacttac tttcgcgtc caaataaag tgaaagtaa 
ctggatatc atcctgtag cggtttttt ctccaaagg 
tatcttgtt aaaggcgct aagatccgt caggggatt 
caactgaag gatataatt gcaaaaata ccactcttg 
tttaagtat tttcccagt taaagtcat taaagtgat 
gcttttgtg tttaatgcc acattcttc aattatcgt 
gcgataaat tttgcacac gtccacttt tttgttgtt 
gatcatgcc actagatgg acctttaca ttcctggat 
ttttttcac ttattaatt attatcgta ttttttttt 
gcaaagtgt tacgtacgt aaatattac ggcataaat 
taccattga tgtacaagg tcgagactt cgaaaaaac 
cgacaaatt ttgttaagg ccaagtagc gcatcaaga 
caaaatttg cgccttcgc aaattaaat tgtgcgctt 
tgtgtggac tgtagaata tgtcttttc gtttgtcgt 
gacgtaagt aatagaaat tttcttgcg agctgacgt 
ataaagctt gttggtcga gtccgtcgt ccatttgga 
cgt
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