Status
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Gene information
General information
TC015204 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1545351..1554407
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015204
    iBeetle screen iB_05774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015204 RA
      MSEPSVNDT SETESLEDE IEPKLKYVR LSNDLQNIL 
      LTSSATCIG VHPKFVCLG SHWGVIHLL DHQGNSIKS 
      KELRPHTVA VNQICIDGS GEYIATCSD DGKVFIHGL 
      FSKENSLHL NVGRLVKTV ALDPLYYKN GANRRFITG 
      DDKLTLYER TFLGSLKPT VLCESEGLV RSLCWGENF 
      IAWSSNIGV RVYDITARC SLGLIKWEE HPGISIDKF 
      RCNLRWADS RTLLIGWVD TVRVCVIRK RSNFEMANR 
      DLPEFLVDP VSTFQTEFY ICGIAPLDH QLVLLGFPK 
      ELDENQKSL RPQLYIVEY RDNDYSDVC TDSLSLRGY 
      EEYTVNDYH LDVLIEENR FFIVAPRDV VIASPYDLD 
      DRIQWFIQH NKFEEALDI LMQSDSRHI HRHTVQSVG 
      IDYLDHLLS REMYDEAGK LCMKIFGKD SNTWEDQIY 
      KFATVHQLR SVSPYIPRT LDSKLNPHI YEMILYEFL 
      KLDAQGFLN LVKEWNPSL YNVSAVINA VLEHLLVCE 
      VDKNLYLEA LAILYSFLK RYDKSLSMY LKLKHKDVF 
      TLIQKHNLY GVIQDMLID LMELDHKKT IALLLEKNS 
      ISSDKIVEK LKPTELHLY RFLDEYDKK NPKGKYHRE 
      LVRLYAIFA REKLLPFLK KSDHYPIQE ALDICKKER 
      FYPEMVYLL GRMGNIEEA LDLIINELK DMQQAISFC 
      QEHDDPDLW NDLINHSLD KPEFIKFLL QSIGTYVDP 
      TVLVQKIRN GMEVPGLKN SLVKMLYQY NLQVSVQEG 
      CKKILVSDY FNLLRKLVK TQQKGIGVS EELMCGACH 
      HMVLEKDPS RCTNITVFN CKHAFHEQC LPENTTRCG 
      ICYPSDK
    CDS sequence
    • >TC015204 RA
      atgtctgag cccagcgtt aacgacacc agcgagact 
      gagagcctt gaggacgaa atcgagcca aaactcaaa 
      tacgtgcgc ttatctaac gacttgcag aacatcctg 
      ttgacgtcc tcggccacc tgcatcggc gtgcacccc 
      aagttcgtg tgtctgggc tcccattgg ggcgtgatc 
      cacctgctg gaccaccaa ggcaacagt atcaagagc 
      aaggagctg cgcccccat acggtggcc gttaaccag 
      atttgcatc gacggcagt ggcgagtac atcgcgacg 
      tgttcggat gatggcaag gttttcatc catggactt 
      ttttcaaag gagaatagt ttgcatttg aatgtgggc 
      agattggtg aagacggtg gctttggac ccgctgtat 
      tacaaaaat ggggccaat cggcggttt atcacaggg 
      gatgataag ttgacgctg tatgagcgc acgtttttg 
      gggagtctt aaaccgact gtcttgtgc gagagcgag 
      gggcttgtg aggagtctt tgttggggg gagaatttt 
      attgcctgg tcgagtaat ataggggtg agggtgtat 
      gatatcacg gcgaggtgc tcgctgggg ttgatcaag 
      tgggaggag cacccggga atttcaatc gataaattt 
      cggtgcaat ttacgatgg gccgatagt cggacccta 
      ttaattggg tgggtggac acggtacga gtttgtgtg 
      atacggaaa cgaagcaat tttgaaatg gccaatcgg 
      gacttaccc gaattttta gtcgaccct gtttcaact 
      ttccaaacc gaattttac atttgtgga attgcgccg 
      ctcgaccac caattagtt ttactcggt ttcccgaaa 
      gaattagac gaaaatcaa aaatcgtta cgtccccaa 
      ctgtacata gtcgaatat cgggacaac gactattcc 
      gatgtttgc accgatagt ttatcactt cgtggttac 
      gaagaatac acagttaac gattaccac ttggatgtt 
      ttaattgaa gaaaatcgc ttctttatt gttgcgcca 
      cgagatgtc gttatagca agtccctat gatctagac 
      gacaggatc cagtggttc atccaacac aataaattt 
      gaggaggct ttggatatt ttaatgcaa agtgacagc 
      cgccatatt caccgacac accgttcag tcggttggc 
      attgactat ctagatcac ttattaagt cgggaaatg 
      tacgacgaa gctggaaaa ttgtgcatg aaaatcttc 
      ggtaaggac tcaaacacg tgggaagac caaatttat 
      aagtttgca accgtgcat caattacga tctgtcagt 
      ccctacatt ccacgcacg cttgattcg aaactaaac 
      ccgcatatt tacgaaatg attttgtac gaatttttg 
      aaactagat gcgcaaggt tttctgaac ttggttaag 
      gaatggaac ccgagtttg tacaacgtt tcggctgtg 
      attaacgcc gtcttggag catcttcta gtgtgtgaa 
      gttgataag aatctatat ttggaagct ttggcgatt 
      ttgtacagt tttttgaag cgttacgac aaaagcttg 
      tccatgtat ttgaagtta aagcacaaa gacgtgttc 
      acactgatc caaaaacac aatttgtac ggtgtgatc 
      caggacatg ctcattgac cttatggag cttgaccat 
      aagaaaact attgcactt ttattagag aaaaactca 
      atttcgagc gataaaatc gtagagaag ctcaaaccg 
      actgaatta catttatac aggttttta gacgagtat 
      gataagaaa aatcctaag gggaagtac caccgtgag 
      ttggtacgc ctgtatgcc attttcgcg cgcgaaaag 
      ttgctcccg tttttgaaa aaatccgac cattatcca 
      atccaggaa gctctggat atttgcaag aaggagagg 
      ttttatccc gaaatggtt tatttgttg ggacggatg 
      ggcaacatc gaagaagcc cttgacttg ataataaac 
      gagttgaaa gacatgcaa caggcgatt tcgttttgt 
      caggaacat gacgatccc gatttgtgg aatgacttg 
      attaatcat tcgttggac aaacctgaa tttattaaa 
      tttctcttg caaagtatc ggaacgtat gtggatccg 
      accgtttta gtacaaaaa attaggaat gggatggaa 
      gtgcccggt ttaaaaaat tcactagtt aaaatgttg 
      tatcagtat aatttacaa gtgtctgta caggaaggg 
      tgtaagaaa attctagtc tcggattat tttaatcta 
      ttacggaag ctcgtcaag acgcaacag aaaggtatt 
      ggcgtgtcg gaagaacta atgtgtggc gcttgtcac 
      cacatggtg ctcgaaaag gacccatcc aggtgtacc 
      aacataaca gttttcaat tgcaagcac gccttccac 
      gaacaatgc ctccctgaa aacaccact cgatgcggc 
      atctgctat ccatccgat aaataa
    mRNA sequence
    • >TC015204 RA
      tttattaaa catttaagt gcgtaaagg tccaatatt 
      accggatat tgaggtatt aatgggcga atttgtgcg 
      tggttggcg accgtgcct gtcaaatca ataatgaat 
      cattagcac aaaatgcaa caattccac aaaaacaac 
      aacaatgtc tgagcccag cgttaacga caccagcga 
      gactgagag ccttgagga cgaaatcga gccaaaact 
      caaatacgt gcgcttatc taacgactt gcagaacat 
      cctgttgac gtcctcggc cacctgcat cggcgtgca 
      ccccaagtt cgtgtgtct gggctccca ttggggcgt 
      gatccacct gctggacca ccaaggcaa cagtatcaa 
      gagcaagga gctgcgccc ccatacggt ggccgttaa 
      ccagatttg catcgacgg cagtggcga gtacatcgc 
      gacgtgttc ggatgatgg caaggtttt catccatgg 
      acttttttc aaaggagaa tagtttgca tttgaatgt 
      gggcagatt ggtgaagac ggtggcttt ggacccgct 
      gtattacaa aaatggggc caatcggcg gtttatcac 
      aggggatga taagttgac gctgtatga gcgcacgtt 
      tttggggag tcttaaacc gactgtctt gtgcgagag 
      cgaggggct tgtgaggag tctttgttg gggggagaa 
      ttttattgc ctggtcgag taatatagg ggtgagggt 
      gtatgatat cacggcgag gtgctcgct ggggttgat 
      caagtggga ggagcaccc gggaatttc aatcgataa 
      atttcggtg caatttacg atgggccga tagtcggac 
      cctattaat tgggtgggt ggacacggt acgagtttg 
      tgtgatacg gaaacgaag caattttga aatggccaa 
      tcgggactt acccgaatt tttagtcga ccctgtttc 
      aactttcca aaccgaatt ttacatttg tggaattgc 
      gccgctcga ccaccaatt agttttact cggtttccc 
      gaaagaatt agacgaaaa tcaaaaatc gttacgtcc 
      ccaactgta catagtcga atatcggga caacgacta 
      ttccgatgt ttgcaccga tagtttatc acttcgtgg 
      ttacgaaga atacacagt taacgatta ccacttgga 
      tgttttaat tgaagaaaa tcgcttctt tattgttgc 
      gccacgaga tgtcgttat agcaagtcc ctatgatct 
      agacgacag gatccagtg gttcatcca acacaataa 
      atttgagga ggctttgga tattttaat gcaaagtga 
      cagccgcca tattcaccg acacaccgt tcagtcggt 
      tggcattga ctatctaga tcacttatt aagtcggga 
      aatgtacga cgaagctgg aaaattgtg catgaaaat 
      cttcggtaa ggactcaaa cacgtggga agaccaaat 
      ttataagtt tgcaaccgt gcatcaatt acgatctgt 
      cagtcccta cattccacg cacgcttga ttcgaaact 
      aaacccgca tatttacga aatgatttt gtacgaatt 
      tttgaaact agatgcgca aggttttct gaacttggt 
      taaggaatg gaacccgag tttgtacaa cgtttcggc 
      tgtgattaa cgccgtctt ggagcatct tctagtgtg 
      tgaagttga taagaatct atatttgga agctttggc 
      gattttgta cagtttttt gaagcgtta cgacaaaag 
      cttgtccat gtatttgaa gttaaagca caaagacgt 
      gttcacact gatccaaaa acacaattt gtacggtgt 
      gatccagga catgctcat tgaccttat ggagcttga 
      ccataagaa aactattgc acttttatt agagaaaaa 
      ctcaatttc gagcgataa aatcgtaga gaagctcaa 
      accgactga attacattt atacaggtt tttagacga 
      gtatgataa gaaaaatcc taaggggaa gtaccaccg 
      tgagttggt acgcctgta tgccatttt cgcgcgcga 
      aaagttgct cccgttttt gaaaaaatc cgaccatta 
      tccaatcca ggaagctct ggatatttg caagaagga 
      gaggtttta tcccgaaat ggtttattt gttgggacg 
      gatgggcaa catcgaaga agcccttga cttgataat 
      aaacgagtt gaaagacat gcaacaggc gatttcgtt 
      ttgtcagga acatgacga tcccgattt gtggaatga 
      cttgattaa tcattcgtt ggacaaacc tgaatttat 
      taaatttct cttgcaaag tatcggaac gtatgtgga 
      tccgaccgt tttagtaca aaaaattag gaatgggat 
      ggaagtgcc cggtttaaa aaattcact agttaaaat 
      gttgtatca gtataattt acaagtgtc tgtacagga 
      agggtgtaa gaaaattct agtctcgga ttattttaa 
      tctattacg gaagctcgt caagacgca acagaaagg 
      tattggcgt gtcggaaga actaatgtg tggcgcttg 
      tcaccacat ggtgctcga aaaggaccc atccaggtg 
      taccaacat aacagtttt caattgcaa gcacgcctt 
      ccacgaaca atgcctccc tgaaaacac cactcgatg 
      cggcatctg ctatccatc cgataaata aaacaacgg 
      taagccaac aaataacta aatatattt ccaaaaatc 
      gtaatcttc atcatcgtc cacatcgaa cggtccttc 
      gaaatattc actctgtgg caaaagggc cggagtaaa 
      agccaggaa atacttggc tgtgaattg gaaaacgtc 
      ctctggttt taattggag gattgtttg gacgtaatc 
      tgagatcat ttcacgcac ttctggctg gtcgtctaa 
      ataattagc gatttgttt tttactcat catctgaaa 
      catttttgt gtttgtgtc ttttttttt caccctaac 
      tttacttac tttcgcgtc caaataaag tgaaagtaa 
      ctggatatc atcctgtag cggtttttt ctccaaagg 
      tatcttgtt aaaggcgct aagatccgt caggggatt 
      caactgaag gatataatt gcaaaaata ccactcttg 
      tttaagtat tttcccagt taaagtcat taaagtgat 
      gcttttgtg tttaatgcc acattcttc aattatcgt 
      gcgataaat tttgcacac gtccacttt tttgttgtt 
      gatcatgcc actagatgg acctttaca ttcctggat 
      ttttttcac ttattaatt attatcgta ttttttttt 
      gcaaagtgt tacgtacgt aaatattac ggcataaat 
      taccattga tgtacaagg tcgagactt cgaaaaaac 
      cgacaaatt ttgttaagg ccaagtagc gcatcaaga 
      caaaatttg cgccttcgc aaattaaat tgtgcgctt 
      tgtgtggac tgtagaata tgtcttttc gtttgtcgt 
      gacgtaagt aatagaaat tttcttgcg agctgacgt 
      ataaagctt gttggtcga gtccgtcgt ccatttgga 
      cgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY