| General information | |||
| TC015204 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 1545351..1554407 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015204 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05774 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015204 RA
MSEPSVNDT SETESLEDE IEPKLKYVR LSNDLQNIL LTSSATCIG VHPKFVCLG SHWGVIHLL DHQGNSIKS KELRPHTVA VNQICIDGS GEYIATCSD DGKVFIHGL FSKENSLHL NVGRLVKTV ALDPLYYKN GANRRFITG DDKLTLYER TFLGSLKPT VLCESEGLV RSLCWGENF IAWSSNIGV RVYDITARC SLGLIKWEE HPGISIDKF RCNLRWADS RTLLIGWVD TVRVCVIRK RSNFEMANR DLPEFLVDP VSTFQTEFY ICGIAPLDH QLVLLGFPK ELDENQKSL RPQLYIVEY RDNDYSDVC TDSLSLRGY EEYTVNDYH LDVLIEENR FFIVAPRDV VIASPYDLD DRIQWFIQH NKFEEALDI LMQSDSRHI HRHTVQSVG IDYLDHLLS REMYDEAGK LCMKIFGKD SNTWEDQIY KFATVHQLR SVSPYIPRT LDSKLNPHI YEMILYEFL KLDAQGFLN LVKEWNPSL YNVSAVINA VLEHLLVCE VDKNLYLEA LAILYSFLK RYDKSLSMY LKLKHKDVF TLIQKHNLY GVIQDMLID LMELDHKKT IALLLEKNS ISSDKIVEK LKPTELHLY RFLDEYDKK NPKGKYHRE LVRLYAIFA REKLLPFLK KSDHYPIQE ALDICKKER FYPEMVYLL GRMGNIEEA LDLIINELK DMQQAISFC QEHDDPDLW NDLINHSLD KPEFIKFLL QSIGTYVDP TVLVQKIRN GMEVPGLKN SLVKMLYQY NLQVSVQEG CKKILVSDY FNLLRKLVK TQQKGIGVS EELMCGACH HMVLEKDPS RCTNITVFN CKHAFHEQC LPENTTRCG ICYPSDK
- >TC015204 RA
atgtctgag cccagcgtt aacgacacc agcgagact gagagcctt gaggacgaa atcgagcca aaactcaaa tacgtgcgc ttatctaac gacttgcag aacatcctg ttgacgtcc tcggccacc tgcatcggc gtgcacccc aagttcgtg tgtctgggc tcccattgg ggcgtgatc cacctgctg gaccaccaa ggcaacagt atcaagagc aaggagctg cgcccccat acggtggcc gttaaccag atttgcatc gacggcagt ggcgagtac atcgcgacg tgttcggat gatggcaag gttttcatc catggactt ttttcaaag gagaatagt ttgcatttg aatgtgggc agattggtg aagacggtg gctttggac ccgctgtat tacaaaaat ggggccaat cggcggttt atcacaggg gatgataag ttgacgctg tatgagcgc acgtttttg gggagtctt aaaccgact gtcttgtgc gagagcgag gggcttgtg aggagtctt tgttggggg gagaatttt attgcctgg tcgagtaat ataggggtg agggtgtat gatatcacg gcgaggtgc tcgctgggg ttgatcaag tgggaggag cacccggga atttcaatc gataaattt cggtgcaat ttacgatgg gccgatagt cggacccta ttaattggg tgggtggac acggtacga gtttgtgtg atacggaaa cgaagcaat tttgaaatg gccaatcgg gacttaccc gaattttta gtcgaccct gtttcaact ttccaaacc gaattttac atttgtgga attgcgccg ctcgaccac caattagtt ttactcggt ttcccgaaa gaattagac gaaaatcaa aaatcgtta cgtccccaa ctgtacata gtcgaatat cgggacaac gactattcc gatgtttgc accgatagt ttatcactt cgtggttac gaagaatac acagttaac gattaccac ttggatgtt ttaattgaa gaaaatcgc ttctttatt gttgcgcca cgagatgtc gttatagca agtccctat gatctagac gacaggatc cagtggttc atccaacac aataaattt gaggaggct ttggatatt ttaatgcaa agtgacagc cgccatatt caccgacac accgttcag tcggttggc attgactat ctagatcac ttattaagt cgggaaatg tacgacgaa gctggaaaa ttgtgcatg aaaatcttc ggtaaggac tcaaacacg tgggaagac caaatttat aagtttgca accgtgcat caattacga tctgtcagt ccctacatt ccacgcacg cttgattcg aaactaaac ccgcatatt tacgaaatg attttgtac gaatttttg aaactagat gcgcaaggt tttctgaac ttggttaag gaatggaac ccgagtttg tacaacgtt tcggctgtg attaacgcc gtcttggag catcttcta gtgtgtgaa gttgataag aatctatat ttggaagct ttggcgatt ttgtacagt tttttgaag cgttacgac aaaagcttg tccatgtat ttgaagtta aagcacaaa gacgtgttc acactgatc caaaaacac aatttgtac ggtgtgatc caggacatg ctcattgac cttatggag cttgaccat aagaaaact attgcactt ttattagag aaaaactca atttcgagc gataaaatc gtagagaag ctcaaaccg actgaatta catttatac aggttttta gacgagtat gataagaaa aatcctaag gggaagtac caccgtgag ttggtacgc ctgtatgcc attttcgcg cgcgaaaag ttgctcccg tttttgaaa aaatccgac cattatcca atccaggaa gctctggat atttgcaag aaggagagg ttttatccc gaaatggtt tatttgttg ggacggatg ggcaacatc gaagaagcc cttgacttg ataataaac gagttgaaa gacatgcaa caggcgatt tcgttttgt caggaacat gacgatccc gatttgtgg aatgacttg attaatcat tcgttggac aaacctgaa tttattaaa tttctcttg caaagtatc ggaacgtat gtggatccg accgtttta gtacaaaaa attaggaat gggatggaa gtgcccggt ttaaaaaat tcactagtt aaaatgttg tatcagtat aatttacaa gtgtctgta caggaaggg tgtaagaaa attctagtc tcggattat tttaatcta ttacggaag ctcgtcaag acgcaacag aaaggtatt ggcgtgtcg gaagaacta atgtgtggc gcttgtcac cacatggtg ctcgaaaag gacccatcc aggtgtacc aacataaca gttttcaat tgcaagcac gccttccac gaacaatgc ctccctgaa aacaccact cgatgcggc atctgctat ccatccgat aaataa
- >TC015204 RA
tttattaaa catttaagt gcgtaaagg tccaatatt accggatat tgaggtatt aatgggcga atttgtgcg tggttggcg accgtgcct gtcaaatca ataatgaat cattagcac aaaatgcaa caattccac aaaaacaac aacaatgtc tgagcccag cgttaacga caccagcga gactgagag ccttgagga cgaaatcga gccaaaact caaatacgt gcgcttatc taacgactt gcagaacat cctgttgac gtcctcggc cacctgcat cggcgtgca ccccaagtt cgtgtgtct gggctccca ttggggcgt gatccacct gctggacca ccaaggcaa cagtatcaa gagcaagga gctgcgccc ccatacggt ggccgttaa ccagatttg catcgacgg cagtggcga gtacatcgc gacgtgttc ggatgatgg caaggtttt catccatgg acttttttc aaaggagaa tagtttgca tttgaatgt gggcagatt ggtgaagac ggtggcttt ggacccgct gtattacaa aaatggggc caatcggcg gtttatcac aggggatga taagttgac gctgtatga gcgcacgtt tttggggag tcttaaacc gactgtctt gtgcgagag cgaggggct tgtgaggag tctttgttg gggggagaa ttttattgc ctggtcgag taatatagg ggtgagggt gtatgatat cacggcgag gtgctcgct ggggttgat caagtggga ggagcaccc gggaatttc aatcgataa atttcggtg caatttacg atgggccga tagtcggac cctattaat tgggtgggt ggacacggt acgagtttg tgtgatacg gaaacgaag caattttga aatggccaa tcgggactt acccgaatt tttagtcga ccctgtttc aactttcca aaccgaatt ttacatttg tggaattgc gccgctcga ccaccaatt agttttact cggtttccc gaaagaatt agacgaaaa tcaaaaatc gttacgtcc ccaactgta catagtcga atatcggga caacgacta ttccgatgt ttgcaccga tagtttatc acttcgtgg ttacgaaga atacacagt taacgatta ccacttgga tgttttaat tgaagaaaa tcgcttctt tattgttgc gccacgaga tgtcgttat agcaagtcc ctatgatct agacgacag gatccagtg gttcatcca acacaataa atttgagga ggctttgga tattttaat gcaaagtga cagccgcca tattcaccg acacaccgt tcagtcggt tggcattga ctatctaga tcacttatt aagtcggga aatgtacga cgaagctgg aaaattgtg catgaaaat cttcggtaa ggactcaaa cacgtggga agaccaaat ttataagtt tgcaaccgt gcatcaatt acgatctgt cagtcccta cattccacg cacgcttga ttcgaaact aaacccgca tatttacga aatgatttt gtacgaatt tttgaaact agatgcgca aggttttct gaacttggt taaggaatg gaacccgag tttgtacaa cgtttcggc tgtgattaa cgccgtctt ggagcatct tctagtgtg tgaagttga taagaatct atatttgga agctttggc gattttgta cagtttttt gaagcgtta cgacaaaag cttgtccat gtatttgaa gttaaagca caaagacgt gttcacact gatccaaaa acacaattt gtacggtgt gatccagga catgctcat tgaccttat ggagcttga ccataagaa aactattgc acttttatt agagaaaaa ctcaatttc gagcgataa aatcgtaga gaagctcaa accgactga attacattt atacaggtt tttagacga gtatgataa gaaaaatcc taaggggaa gtaccaccg tgagttggt acgcctgta tgccatttt cgcgcgcga aaagttgct cccgttttt gaaaaaatc cgaccatta tccaatcca ggaagctct ggatatttg caagaagga gaggtttta tcccgaaat ggtttattt gttgggacg gatgggcaa catcgaaga agcccttga cttgataat aaacgagtt gaaagacat gcaacaggc gatttcgtt ttgtcagga acatgacga tcccgattt gtggaatga cttgattaa tcattcgtt ggacaaacc tgaatttat taaatttct cttgcaaag tatcggaac gtatgtgga tccgaccgt tttagtaca aaaaattag gaatgggat ggaagtgcc cggtttaaa aaattcact agttaaaat gttgtatca gtataattt acaagtgtc tgtacagga agggtgtaa gaaaattct agtctcgga ttattttaa tctattacg gaagctcgt caagacgca acagaaagg tattggcgt gtcggaaga actaatgtg tggcgcttg tcaccacat ggtgctcga aaaggaccc atccaggtg taccaacat aacagtttt caattgcaa gcacgcctt ccacgaaca atgcctccc tgaaaacac cactcgatg cggcatctg ctatccatc cgataaata aaacaacgg taagccaac aaataacta aatatattt ccaaaaatc gtaatcttc atcatcgtc cacatcgaa cggtccttc gaaatattc actctgtgg caaaagggc cggagtaaa agccaggaa atacttggc tgtgaattg gaaaacgtc ctctggttt taattggag gattgtttg gacgtaatc tgagatcat ttcacgcac ttctggctg gtcgtctaa ataattagc gatttgttt tttactcat catctgaaa catttttgt gtttgtgtc ttttttttt caccctaac tttacttac tttcgcgtc caaataaag tgaaagtaa ctggatatc atcctgtag cggtttttt ctccaaagg tatcttgtt aaaggcgct aagatccgt caggggatt caactgaag gatataatt gcaaaaata ccactcttg tttaagtat tttcccagt taaagtcat taaagtgat gcttttgtg tttaatgcc acattcttc aattatcgt gcgataaat tttgcacac gtccacttt tttgttgtt gatcatgcc actagatgg acctttaca ttcctggat ttttttcac ttattaatt attatcgta ttttttttt gcaaagtgt tacgtacgt aaatattac ggcataaat taccattga tgtacaagg tcgagactt cgaaaaaac cgacaaatt ttgttaagg ccaagtagc gcatcaaga caaaatttg cgccttcgc aaattaaat tgtgcgctt tgtgtggac tgtagaata tgtcttttc gtttgtcgt gacgtaagt aatagaaat tttcttgcg agctgacgt ataaagctt gttggtcga gtccgtcgt ccatttgga cgt
