Status
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Gene information
General information
TC015198 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1629977..1635897
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015198
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05772

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: In most case it is very difficult to identify head appendages. Labium seem to be missing most of the time, while anttena, maxilla, mandible are always present. Labrum are sometimes visible, sometimes not.
    larva ventral not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: In many cases, the heads look like bent ventrally. However this might be the secondary defect of the constricted head and thorax defect.
    head constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015198 RA
      MDKYTVDLD QVLNDFEYS ELTDQHSSN HKQNFNNFK 
      NSNVKHSIN NVFHSLNEY LNTDISPKC EPILPETGE 
      NSANNAPEE VTQSDENHV IEAENGEIN HVVEEETEI 
      ISLVEDKNE ESDQEESEE TVEVESRDS PPVEENQVA 
      LVATNESIE QKTDEPSDL ESEEKGDTG VSKVGFDDD 
      IDLDESELN RYLDELEQE YDNTTPKTS EEDETTSSV 
      RPNNLPLPQ QEDEKPVID LVGEPGSTP YNNVYVNKE 
      LPQEVKQNS DTECSTSAS PTFSDISSE TNSSCTPST 
      DTVEEPARE AAPTEPPEG GAGDQLEPP QVSDATLGK 
      QAPVWIPDS EALNCQHCD TKFTVIKRR HHCRACGYV 
      LCSKCCNLK YRLEYLDDV ARVCTKCYE QLEKAATVT 
      NSSDSESNS PRSQPNPNN PLEYCSTVP PHQQVAGNV 
      APPAVMVPV GVLKRKGSS KRTNKSVMF CDGIRPGSD 
      LTNLDNDFN YSGVKGGEK NFPVVDPET NSFIPKGDD 
      LPPTVTINK SDVSYLECS NGPNVVEML KNETLIFSL 
      QTNLLVHVK IINMDCCIN KTAWCFSTE GLINVGQDE 
      IVILLELLD DEKTVPKDV FFHLNYIYL DAVKGTSVK 
      EMGLSLHST TNFLGSKNH AGFVYIRPT FQCLCNVIK 
      PQEPFLIGV LIHRWETSW AKLFPLRLI LRLGAEYRY 
      YPSPIISNR YRDSVFVEI GHTIINLLA DFRNFSYTL 
      PQIRGLSIH MEDKNTTVT IPSNRYDQV MKSMNNSSD 
      HILAFAGNF SCLADSHLV CIQDTQSNE NTYSTHAIN 
      IHNKPRKVT GASFIVFNG ALKSSSGLT AKSSIVEDG 
      LMIQIPSEH MTQIRESLR NMKNHTIQC GCINATSDE 
      TVNIVWGEP DSNFNVGVF SPIDNKSME GIPSIRVHN 
      GKDYVNGGR FIRWTEVFI VQNEGEASR NQDPIDISK 
      VSETIAKAT CTALVKYLD LLISNSFYK IGIRANLNA 
      LFVAYCAGS NGVKLPPIY MESLDNELV PVLHKHATS 
      DCDENTIIL ELIFRILNK
    CDS sequence
    • >TC015198 RA
      atggataaa tacacggtg gatttagac caggttttg 
      aacgatttc gaatatagc gaactaacc gaccaacac 
      agttcaaac cacaagcaa aatttcaac aatttcaaa 
      aactcgaat gtcaaacat tcgatcaac aacgtcttt 
      cacagcctg aacgagtat ttaaacacc gatatttca 
      cccaaatgt gagccgatt ttgcccgag actggcgaa 
      aattccgcc aataatgcg cccgaagag gtcactcaa 
      agtgacgaa aaccacgta attgaggcc gaaaacggc 
      gaaattaac cacgtagtt gaggaagaa actgaaata 
      atctcacta gtagaggat aagaacgag gaaagtgat 
      caggaagaa tctgaagaa acggttgaa gtcgagtca 
      cgcgactcg cccccggtt gaggaaaat caagtcgct 
      ttggttgca acaaacgag tcaattgaa caaaagact 
      gacgaacca agtgacctt gagagtgaa gaaaagggc 
      gacacaggt gtgtccaaa gtaggcttt gatgacgac 
      attgattta gatgagtcg gaattgaac cgatatttg 
      gacgaattg gagcaagaa tatgacaat acaacaccc 
      aaaacaagc gaagaagac gaaacaaca tcaagtgtg 
      cggcctaac aacctgcca ttgccacaa caagaggac 
      gaaaaaccg gtcatagac ctagtgggc gagcctggt 
      tcaactccc tataacaac gtttacgta aataaggag 
      ttgccccag gaagtgaaa caaaacagt gatactgaa 
      tgttcaact tccgcttcc cccactttt tcggacatt 
      tccagtgag acaaattcg tcgtgcacg ccctccacc 
      gacacggtg gaggagccg gcacgggag gcggcccca 
      accgaaccc cccgaaggg ggtgccggt gaccaactg 
      gagcccccg caagtttca gacgctacg ttgggtaaa 
      caagccccc gtgtggatc cccgattcg gaagcgctc 
      aattgccaa cattgcgac actaaattc actgtaatc 
      aaacgtcgc caccactgt cgtgcctgt ggttacgtc 
      ctgtgttcg aaatgttgt aaccttaag tacaggctt 
      gagtatctc gacgatgtg gcacgagtt tgtacgaag 
      tgttacgaa cagttggag aaagccgcc acggttacc 
      aatagttca gacagcgag tcaaattcc cccagatcg 
      caaccgaat ccgaataat ccacttgag tattgttca 
      actgtacca ccacatcag caagtggca gggaatgtg 
      gcaccgcct gctgtgatg gtgccagtt ggggtgctt 
      aagaggaaa gggtcgagt aaaaggact aataaaagt 
      gtgatgttt tgcgatgga attaggccc gggagtgat 
      ttaaccaat ctggataat gattttaat tacagtggg 
      gtcaaaggg ggcgagaaa aattttccg gttgttgat 
      ccggaaact aatagtttt atacccaag ggggacgat 
      ttgcccccg actgtcacc ataaacaaa tcagatgtg 
      tcgtatttg gagtgcagt aacgggcca aacgtggtt 
      gaaatgttg aaaaacgag actttgatt ttttctctg 
      caaactaat ctccttgtt cacgtcaag ataattaac 
      atggattgt tgtataaac aagacggcg tggtgtttt 
      tccaccgaa ggcttgata aatgtaggt caggacgaa 
      atagtaatt cttctcgaa ttactagat gatgaaaaa 
      accgttccg aaggatgtt ttttttcat ttgaattac 
      atttatttg gatgcggtt aaaggcacg tctgttaaa 
      gaaatgggc ttgtcgttg cattcaaca acgaatttt 
      ttgggctcg aaaaatcat gccggtttt gtgtacatt 
      cggccaaca ttccagtgt ttatgtaac gtgataaag 
      ccgcaagag ccgtttctg attggtgtt ttgatacat 
      aggtgggaa acatcttgg gcgaaattg tttcctttg 
      aggcttatt ttgagacta ggggctgag tataggtat 
      tatcccagt ccgattatt tcaaatcgg tacagggac 
      agtgttttt gtcgaaatt ggacacacg atcattaat 
      ttgcttgct gatttccgg aacttctct tacaccctg 
      ccccaaatc cggggcctc agcatccac atggaggac 
      aagaacacc actgtgaca atcccctcg aatcgctac 
      gaccaggtg atgaaatcg atgaacaat tccagcgac 
      cacattttg gcatttgct ggaaatttc agctgtttg 
      gccgactca catctagtg tgcattcaa gacacgcaa 
      agtaacgaa aacacttac agcacgcat gcgattaac 
      atacacaat aagccgcgc aaagtgact ggggcgagt 
      tttattgtt tttaacggc gcgttgaaa tcatcaagt 
      ggcctaacg gcgaaatcg tcaattgtc gaggacggc 
      ttgatgatt caaatcccc tccgaacat atgacgcaa 
      attcgcgaa agtttgcga aatatgaag aaccacacc 
      atacagtgt ggttgcatt aatgcgacg tctgatgag 
      acggttaat atcgtgtgg ggggagcct gactcgaat 
      ttcaacgtt ggggttttt tcgcccatt gataacaaa 
      tcgatggag gggattccc tcaattcgg gttcataac 
      gggaaggat tacgtcaat gggggccgg tttattcgc 
      tggactgag gtttttatt gtacagaat gagggtgaa 
      gcgagccgt aatcaggac cccattgac atttccaaa 
      gtgtcggag actatagct aaagccacg tgtacggca 
      cttgtcaag tatttggat ctgttgata agtaatagt 
      ttttataaa attgggatt agggccaat cttaatgca 
      ctttttgtg gcgtactgc gcggggagt aatggagtg 
      aagttgccg ccgatttat atggagagt ttggataat 
      gaattggtt cctgtgctt cacaagcac gcgactagc 
      gattgtgac gaaaacaca ataattctg gaattaata 
      ttccgcatt ttgaataaa tag
    mRNA sequence
    • >TC015198 RA
      tcagctgat tgtgatatt ttagtgtgg ttataaaag 
      ccaaaaaag tgggttttt tgtttatat ttatttatt 
      ttcaaatag tgcaatgaa atatctggg ttatttaat 
      aaccggaaa tggataaat acacggtgg atttagacc 
      aggttttga acgatttcg aatatagcg aactaaccg 
      accaacaca gttcaaacc acaagcaaa atttcaaca 
      atttcaaaa actcgaatg tcaaacatt cgatcaaca 
      acgtctttc acagcctga acgagtatt taaacaccg 
      atatttcac ccaaatgtg agccgattt tgcccgaga 
      ctggcgaaa attccgcca ataatgcgc ccgaagagg 
      tcactcaaa gtgacgaaa accacgtaa ttgaggccg 
      aaaacggcg aaattaacc acgtagttg aggaagaaa 
      ctgaaataa tctcactag tagaggata agaacgagg 
      aaagtgatc aggaagaat ctgaagaaa cggttgaag 
      tcgagtcac gcgactcgc ccccggttg aggaaaatc 
      aagtcgctt tggttgcaa caaacgagt caattgaac 
      aaaagactg acgaaccaa gtgaccttg agagtgaag 
      aaaagggcg acacaggtg tgtccaaag taggctttg 
      atgacgaca ttgatttag atgagtcgg aattgaacc 
      gatatttgg acgaattgg agcaagaat atgacaata 
      caacaccca aaacaagcg aagaagacg aaacaacat 
      caagtgtgc ggcctaaca acctgccat tgccacaac 
      aagaggacg aaaaaccgg tcatagacc tagtgggcg 
      agcctggtt caactccct ataacaacg tttacgtaa 
      ataaggagt tgccccagg aagtgaaac aaaacagtg 
      atactgaat gttcaactt ccgcttccc ccacttttt 
      cggacattt ccagtgaga caaattcgt cgtgcacgc 
      cctccaccg acacggtgg aggagccgg cacgggagg 
      cggccccaa ccgaacccc ccgaagggg gtgccggtg 
      accaactgg agcccccgc aagtttcag acgctacgt 
      tgggtaaac aagcccccg tgtggatcc ccgattcgg 
      aagcgctca attgccaac attgcgaca ctaaattca 
      ctgtaatca aacgtcgcc accactgtc gtgcctgtg 
      gttacgtcc tgtgttcga aatgttgta accttaagt 
      acaggcttg agtatctcg acgatgtgg cacgagttt 
      gtacgaagt gttacgaac agttggaga aagccgcca 
      cggttacca atagttcag acagcgagt caaattccc 
      ccagatcgc aaccgaatc cgaataatc cacttgagt 
      attgttcaa ctgtaccac cacatcagc aagtggcag 
      ggaatgtgg caccgcctg ctgtgatgg tgccagttg 
      gggtgctta agaggaaag ggtcgagta aaaggacta 
      ataaaagtg tgatgtttt gcgatggaa ttaggcccg 
      ggagtgatt taaccaatc tggataatg attttaatt 
      acagtgggg tcaaagggg gcgagaaaa attttccgg 
      ttgttgatc cggaaacta atagtttta tacccaagg 
      gggacgatt tgcccccga ctgtcacca taaacaaat 
      cagatgtgt cgtatttgg agtgcagta acgggccaa 
      acgtggttg aaatgttga aaaacgaga ctttgattt 
      tttctctgc aaactaatc tccttgttc acgtcaaga 
      taattaaca tggattgtt gtataaaca agacggcgt 
      ggtgttttt ccaccgaag gcttgataa atgtaggtc 
      aggacgaaa tagtaattc ttctcgaat tactagatg 
      atgaaaaaa ccgttccga aggatgttt tttttcatt 
      tgaattaca tttatttgg atgcggtta aaggcacgt 
      ctgttaaag aaatgggct tgtcgttgc attcaacaa 
      cgaattttt tgggctcga aaaatcatg ccggttttg 
      tgtacattc ggccaacat tccagtgtt tatgtaacg 
      tgataaagc cgcaagagc cgtttctga ttggtgttt 
      tgatacata ggtgggaaa catcttggg cgaaattgt 
      ttcctttga ggcttattt tgagactag gggctgagt 
      ataggtatt atcccagtc cgattattt caaatcggt 
      acagggaca gtgtttttg tcgaaattg gacacacga 
      tcattaatt tgcttgctg atttccgga acttctctt 
      acaccctgc cccaaatcc ggggcctca gcatccaca 
      tggaggaca agaacacca ctgtgacaa tcccctcga 
      atcgctacg accaggtga tgaaatcga tgaacaatt 
      ccagcgacc acattttgg catttgctg gaaatttca 
      gctgtttgg ccgactcac atctagtgt gcattcaag 
      acacgcaaa gtaacgaaa acacttaca gcacgcatg 
      cgattaaca tacacaata agccgcgca aagtgactg 
      gggcgagtt ttattgttt ttaacggcg cgttgaaat 
      catcaagtg gcctaacgg cgaaatcgt caattgtcg 
      aggacggct tgatgattc aaatcccct ccgaacata 
      tgacgcaaa ttcgcgaaa gtttgcgaa atatgaaga 
      accacacca tacagtgtg gttgcatta atgcgacgt 
      ctgatgaga cggttaata tcgtgtggg gggagcctg 
      actcgaatt tcaacgttg gggtttttt cgcccattg 
      ataacaaat cgatggagg ggattccct caattcggg 
      ttcataacg ggaaggatt acgtcaatg ggggccggt 
      ttattcgct ggactgagg tttttattg tacagaatg 
      agggtgaag cgagccgta atcaggacc ccattgaca 
      tttccaaag tgtcggaga ctatagcta aagccacgt 
      gtacggcac ttgtcaagt atttggatc tgttgataa 
      gtaatagtt tttataaaa ttgggatta gggccaatc 
      ttaatgcac tttttgtgg cgtactgcg cggggagta 
      atggagtga agttgccgc cgatttata tggagagtt 
      tggataatg aattggttc ctgtgcttc acaagcacg 
      cgactagcg attgtgacg aaaacacaa taattctgg 
      aattaatat tccgcattt tgaataaat agtattggt 
      gtaattgtt aatttatac aaattttat ttgttattt 
      taattaata aatataact aaccaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY