Status
Please wait ...
Gene information
General information
TC034727 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2048882..2053408
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034727
    iBeetle screen iB_05769

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034727 RA
      MPASIGVNL RVTGHCRQG GRKYMEDFF SVAYQQTED 
      ERDLEYAFF GIYDGHGGA EAAAFAKEH LMETIIKHK 
      TFWSDNDED VLRAIKDGY IATHYAMWK EQEKWPRTA 
      SGLPSTAGT TASVAFIRR GKVYIGHVG DSGIILGYQ 
      EEGSTEWRA KPLTRDHKP ENLDEMTRI NQCGGKVVS 
      KSGVPRVVW NRPKMGHQG PVRRSTPID EIPFLAVAR 
      SLGDLWSYN SQINQFIVS PDPDCAVFT IDTSTHRCL 
      VFGTDGLFN MLSPQNAVH IVQQAERHN ENAALSDSI 
      NKTWLNPSK LLVEKALER WHNTKMRAD NTSVVTLML 
      DPPGPPRAQ VLKNRKKNA YPDTGLEIM TRYEEGLF
    CDS sequence
    • >TC034727 RA
      atgcccgct tcgatcgga gttaatctg cgcgtgacg 
      gggcactgc cgccaaggg ggccgcaag tacatggag 
      gacttcttc tcggtggcg taccagcag acggaggac 
      gagcgcgac ctggagtac gccttcttc ggcatctac 
      gacgggcac gggggcgcc gaggcggcg gccttcgcc 
      aaggagcac ctcatggag acgatcatc aagcacaag 
      accttctgg tcggacaac gacgaggac gtgctgcgg 
      gccatcaag gacggctat atcgccaca cactacgcc 
      atgtggaag gagcaagag aagtggccg cggacggcg 
      tcggggctg ccgagcacg gcggggacc acggctagt 
      gtggcgttc atacgcagg ggcaaagtc tacattggg 
      catgtgggg gattcgggg attattcta gggtatcag 
      gaggagggc tcgaccgag tggagggcg aagcctctg 
      acaagggac cacaagcca gagaatttg gacgagatg 
      acgaggatt aatcagtgt ggcgggaaa gttgtctcg 
      aaatcaggc gtgccgcgc gtggtgtgg aaccgcccc 
      aaaatgggg caccagggc cccgtgcgt cgctccacc 
      cccatcgac gagatcccc ttcctggcc gtggcgcga 
      agccttggc gacttatgg tcttacaac tcccaaatc 
      aaccaattc atcgtatcc cccgacccc gactgtgcc 
      gtcttcaca atcgatacc tcgacccac cgctgtctc 
      gtcttcggc accgacggc ctcttcaac atgctcagc 
      ccccagaac gccgtgcac atcgtccag caggcggaa 
      cgccataac gaaaacgcc gccctcagc gactcgatc 
      aacaaaacg tggctcaac cccagcaag ctcctggtg 
      gagaaggcg ctggagcgc tggcacaac accaagatg 
      cgggccgac aacacctcg gtggtcacg ctcatgctg 
      gacccccca ggtcccccg agggcccaa gtgctgaaa 
      aaccgcaag aaaaacgcg tatccagac accgggctg 
      gagataatg acgcggtat gaggagggg cttttttaa
    mRNA sequence
    • >TC034727 RA
      agtaggcgt caagccgcg acgtacgtc cggcgaaag 
      tcgcgtgcg tgcgagcga gacggcgct agtggcgcg 
      cgtcgcagc gtttgacac agttaaaat tgacaatga 
      cagttacgc cacaaacaa tatcgaaaa ttcgaatca 
      aaaattgtc taaagcgtg ttttctagt acaagtggc 
      gttttaatg tattgcttt acacaatgc ccgcttcga 
      tcggagtta atctgcgcg tgacggggc actgccgcc 
      aagggggcc gcaagtaca tggaggact tcttctcgg 
      tggcgtacc agcagacgg aggacgagc gcgacctgg 
      agtacgcct tcttcggca tctacgacg ggcacgggg 
      gcgccgagg cggcggcct tcgccaagg agcacctca 
      tggagacga tcatcaagc acaagacct tctggtcgg 
      acaacgacg aggacgtgc tgcgggcca tcaaggacg 
      gctatatcg ccacacact acgccatgt ggaaggagc 
      aagagaagt ggccgcgga cggcgtcgg ggctgccga 
      gcacggcgg ggaccacgg ctagtgtgg cgttcatac 
      gcaggggca aagtctaca ttgggcatg tgggggatt 
      cggggatta ttctagggt atcaggagg agggctcga 
      ccgagtgga gggcgaagc ctctgacaa gggaccaca 
      agccagaga atttggacg agatgacga ggattaatc 
      agtgtggcg ggaaagttg tctcgaaat caggcgtgc 
      cgcgcgtgg tgtggaacc gccccaaaa tggggcacc 
      agggccccg tgcgtcgct ccaccccca tcgacgaga 
      tccccttcc tggccgtgg cgcgaagcc ttggcgact 
      tatggtctt acaactccc aaatcaacc aattcatcg 
      tatcccccg accccgact gtgccgtct tcacaatcg 
      atacctcga cccaccgct gtctcgtct tcggcaccg 
      acggcctct tcaacatgc tcagccccc agaacgccg 
      tgcacatcg tccagcagg cggaacgcc ataacgaaa 
      acgccgccc tcagcgact cgatcaaca aaacgtggc 
      tcaacccca gcaagctcc tggtggaga aggcgctgg 
      agcgctggc acaacacca agatgcggg ccgacaaca 
      cctcggtgg tcacgctca tgctggacc ccccaggtc 
      ccccgaggg cccaagtgc tgaaaaacc gcaagaaaa 
      acgcgtatc cagacaccg ggctggaga taatgacgc 
      ggtatgagg aggggcttt tttaatttt ttaatctaa 
      gatgggaaa catctacgt gatttttta tacataata 
      tattttagt tccgtatat ttttctcaa gtgttcaca 
      tgagcttta ggtttgtgt tattgcgaa tgtgttgtt 
      cttttaggt tttgtcatg gaaacacgt ctaaaacat 
      ttttgtgcc tttgttacc aagtgttgg aaaaataaa 
      tgtgttaat aaacttagg cgaaacaaa gaaatgtat 
      atttataat aaatgagga aatgtatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY