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Gene information
General information
TC015163 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2148699..2153773
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015163
    iBeetle screen iB_05767

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015163 RA
      MADTFFGFD TSINNGDHL LPPDDDDCV ENEEEEYDA 
      LNDETFGGL EDSSVLDDW EQQHEQYAE IAESTKHSE 
      QLENSISRL MLEDNGPLN LSKNSVWAY TPPNKNGDM 
      FNSSILSSL QKASKSFIE SHSREAPLP SIPLSPIHQ 
      VPKQIRTVE ELEKNLIKS SKQQPPIKP QVPFPHPLF 
      RLPPGLPPI GIPPPVRHM LPHHPVPPG LVHMIPPHF 
      MQGGPPRHL IYPQGNAQF PVNHPYNFP PPPRNVMPP 
      NAAANHVRM QRTRNDSYR SRENNENKD EYAGLMTSR 
      EKQWLLNIQ MLQLNTGTP YFDDYYYTV FKERKAKNN 
      KENFPNRYQ NNRQRNNER QDNNNTLTP RVYTPLQFE 
      NSLGKLQCG SVTAPRKII DMDIVTPDK DSDSPVVVR 
      DTRKTKQLL LELEALFTL LLKAEDLRN PLAISNMEK 
      LHEIKQKQR LRELDLAPT PEQKQEVLK LLKQDSEPV 
      VENPHDYIV KIVNGLLQE DKFASFLNI RKGKMLLLR 
      LLPHLTMDG FSAQLLEIW TRILLSIAI VGRRDTAGD 
      HILPKLHPF FKRYIQTST MGDILEIVT GLVEVIRQE 
      NSRSTPLSH QGKAPLYFV LLNKFGVSA LVTMLIRTE 
      YLISSETAS EKQQNDWSN FLVSWADLT GVVSKVATP 
      IEPVPIQIF TKHCNRFEN LTPDKKMQL EKHFVDSN
    CDS sequence
    • >TC015163 RA
      atggctgat acgtttttt ggttttgat acgtcgata 
      aataacgga gatcatttg ttgccgccg gatgacgac 
      gactgtgtc gagaacgag gaggaggag tatgacgct 
      cttaacgat gagaccttc ggggggctc gaagacagc 
      tcggtcttg gacgactgg gagcagcag cacgagcaa 
      tacgccgaa attgcggag agcacgaag cactccgaa 
      cagttggag aactcaatt tcacgtttg atgctcgaa 
      gacaatggc ccccttaat ttatcgaaa aattcggtc 
      tgggcgtac accccccct aacaaaaat ggggacatg 
      ttcaattcg tcgattctg tcgtccttg cagaaagca 
      tcgaagtct tttatcgag tctcattca cgtgaggcc 
      ccccttccc tcaatcccc ctttcgccc atccaccaa 
      gtccccaag caaatacgt acagttgaa gaattagaa 
      aaaaatcta atcaaaagt tcaaaacaa cagccccct 
      atcaagcca caagtccct ttccctcac ccacttttc 
      cgactaccg ccggggctc cccccgatt ggtataccg 
      ccccctgtg agacacatg ctgccccac catcccgtg 
      ccccctggc ttggttcac atgatcccg ccgcatttt 
      atgcaaggg ggaccgcca agacacttg atttatcca 
      caaggcaat gcacaattt ccggtgaat cacccgtat 
      aattttcca ccgccgcct agaaacgta atgccgcca 
      aatgcagcc gccaatcat gtgcgcatg caaaggacg 
      aggaacgat agttataga agtagggaa aataatgaa 
      aataaggac gagtatgcc ggcttgatg acaagtagg 
      gaaaaacag tggctttta aatattcaa atgttgcaa 
      ttaaacacc ggaacgccg tatttcgac gactattat 
      tacacggtt tttaaagag cggaaagcg aagaataat 
      aaagagaat tttccgaat aggtatcag aataatagg 
      cagagaaat aacgagaga caggataac aacaatacc 
      ttgaccccg agggtttat acaccgttg cagtttgaa 
      aattcctta gggaaatta cagtgtggg tcggtcact 
      gcccctcga aaaatcatc gacatggac atagtaacc 
      cccgacaag gactccgac agccccgtc gtggtccga 
      gacacgcgt aaaaccaag caactcctc cttgaactc 
      gaagctctc ttcacgctc ctcctcaaa gccgaagac 
      ctacgtaac cctctcgca atcagcaac atggaaaaa 
      ctgcacgaa atcaaacaa aaacaacgt ttacgtgag 
      ctagattta gccccaaca cccgaacaa aaacaagaa 
      gttttgaaa cttttaaag caagacagc gaaccagtt 
      gtcgaaaat ccgcacgat tatatcgtg aaaatcgtc 
      aacggtttg ctgcaggaa gacaaattt gccagtttt 
      ttgaacata agaaaaggg aaaatgttg ctgttgagg 
      cttttgcca catttgact atggacggt ttttccgcc 
      cagttgttg gagatttgg acgcggatt ttgttgagt 
      attgccatt gttgggagg agagatacg gcaggcgat 
      cacattttg cccaagttg catccgttt ttcaagagg 
      tacatacag actagtacg atgggtgat atcttggaa 
      attgtgacg ggattggtt gaggtcatc aggcaggag 
      aacagcaga agcacgccg ttgagtcac caaggcaaa 
      gcgccgctt tatttcgtc ctcttgaat aaatttggt 
      gtctctgca ctggtgacg atgttgata cgaaccgaa 
      tacctgatt tcgtccgag acagcgagc gagaaacaa 
      cagaacgac tggtccaat tttctagtg agctgggcc 
      gacctgaca ggagtggtc agtaaagtg gcgacacct 
      atcgagccc gttccgatt cagattttc accaagcat 
      tgcaaccgc ttcgagaat ctaacaccc gataagaaa 
      atgcagttg gagaagcac tttgtcgac tccaactga
    mRNA sequence
    • >TC015163 RA
      aacggtgtt attaagcat aatgattga aaatattca 
      aaaatcaaa tggctgata cgttttttg gttttgata 
      cgtcgataa ataacggag atcatttgt tgccgccgg 
      atgacgacg actgtgtcg agaacgagg aggaggagt 
      atgacgctc ttaacgatg agaccttcg gggggctcg 
      aagacagct cggtcttgg acgactggg agcagcagc 
      acgagcaat acgccgaaa ttgcggaga gcacgaagc 
      actccgaac agttggaga actcaattt cacgtttga 
      tgctcgaag acaatggcc cccttaatt tatcgaaaa 
      attcggtct gggcgtaca cccccccta acaaaaatg 
      gggacatgt tcaattcgt cgattctgt cgtccttgc 
      agaaagcat cgaagtctt ttatcgagt ctcattcac 
      gtgaggccc cccttccct caatccccc tttcgccca 
      tccaccaag tccccaagc aaatacgta cagttgaag 
      aattagaaa aaaatctaa tcaaaagtt caaaacaac 
      agcccccta tcaagccac aagtccctt tccctcacc 
      cacttttcc gactaccgc cggggctcc ccccgattg 
      gtataccgc cccctgtga gacacatgc tgccccacc 
      atcccgtgc cccctggct tggttcaca tgatcccgc 
      cgcatttta tgcaagggg gaccgccaa gacacttga 
      tttatccac aaggcaatg cacaatttc cggtgaatc 
      acccgtata attttccac cgccgccta gaaacgtaa 
      tgccgccaa atgcagccg ccaatcatg tgcgcatgc 
      aaaggacga ggaacgata gttatagaa gtagggaaa 
      ataatgaaa ataaggacg agtatgccg gcttgatga 
      caagtaggg aaaaacagt ggcttttaa atattcaaa 
      tgttgcaat taaacaccg gaacgccgt atttcgacg 
      actattatt acacggttt ttaaagagc ggaaagcga 
      agaataata aagagaatt ttccgaata ggtatcaga 
      ataataggc agagaaata acgagagac aggataaca 
      acaatacct tgaccccga gggtttata caccgttgc 
      agtttgaaa attccttag ggaaattac agtgtgggt 
      cggtcactg cccctcgaa aaatcatcg acatggaca 
      tagtaaccc ccgacaagg actccgaca gccccgtcg 
      tggtccgag acacgcgta aaaccaagc aactcctcc 
      ttgaactcg aagctctct tcacgctcc tcctcaaag 
      ccgaagacc tacgtaacc ctctcgcaa tcagcaaca 
      tggaaaaac tgcacgaaa tcaaacaaa aacaacgtt 
      tacgtgagc tagatttag ccccaacac ccgaacaaa 
      aacaagaag ttttgaaac ttttaaagc aagacagcg 
      aaccagttg tcgaaaatc cgcacgatt atatcgtga 
      aaatcgtca acggtttgc tgcaggaag acaaatttg 
      ccagttttt tgaacataa gaaaaggga aaatgttgc 
      tgttgaggc ttttgccac atttgacta tggacggtt 
      tttccgccc agttgttgg agatttgga cgcggattt 
      tgttgagta ttgccattg ttgggagga gagatacgg 
      caggcgatc acattttgc ccaagttgc atccgtttt 
      tcaagaggt acatacaga ctagtacga tgggtgata 
      tcttggaaa ttgtgacgg gattggttg aggtcatca 
      ggcaggaga acagcagaa gcacgccgt tgagtcacc 
      aaggcaaag cgccgcttt atttcgtcc tcttgaata 
      aatttggtg tctctgcac tggtgacga tgttgatac 
      gaaccgaat acctgattt cgtccgaga cagcgagcg 
      agaaacaac agaacgact ggtccaatt ttctagtga 
      gctgggccg acctgacag gagtggtca gtaaagtgg 
      cgacaccta tcgagcccg ttccgattc agattttca 
      ccaagcatt gcaaccgct tcgagaatc taacacccg 
      ataagaaaa tgcagttgg agaagcact ttgtcgact 
      ccaactgat caatctact aatccatat atttttact 
      ttttatgtt tttagatag gcgtcaaga actgtaaaa 
      aaggtgtac aacttattt tattgtttt tatatatga 
      aagagagaa gttagatgt tgaaatttg ttatttgta 
      acaactctt gattgttgg ttgtagctt tttaattat 
      gtgtaattc aattaatta tttttgtat attaaatta 
      gtaatttat tggatttgt tatggaaat tgcacgact 
      cgcatccac cgctttgat tttattgtg acgtatccg 
      aaattcgtt atcgtgagt ttgccaatt gagcggccg 
      atgctggtt cattaattg ggctaatgc aaaatattt 
      gttttcaat tgcattagt tggaattaa ctttagaca 
      attaagtaa atttaacag aaccgttta ttttccaga 
      tatctaaat atgtttgaa agatatcat gctttttca 
      atagatagt gaatgtcaa acggatata tgttaataa 
      aaagtttga cccaactgt tgtttgaat ttcccgccc 
      gcgccgcgg cgccctcta gcgacgcca ccgcgccag 
      tgacagccg cgacattgt cataaccaa gaaattaat 
      aaaacatta cagaaaaaa cgcgaattt aatgaaaat 
      tgtgtgttt tcggcgacg attaggcca cgacgagcg 
      actttatct cgacatttg gggtaagtg ctattttta 
      gcgcgctta tcagttgaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY