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Gene information
General information
TC015154 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2305117..2306674
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015154
    iBeetle screen iB_05764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015154 RA
      MSKKLCLLF LITLGIICT FVISKNLTS DQTPTKHHT 
      FRQILQAPS DPLDKPFSL PPAKNTTGN IPSVFFTPK 
      APKPTKLNT TCAKFPDLF DLQFRNDHW QLQKTSTGS 
      LFLFNAYFD RRGGDFVRI ITMFTQHPP ETQFWCQFW 
      YQNSTKPVI VESEAPFWL FSRKWGADR GQFVHPYLI 
      KCPVKGAPF GVSLVEKPC DNASNVLQI FGKETVVKK 
      NFVVCVKGL NFPFEDKSL RLVEWIELL QILGVEKIN 
      FYEFRVHPN TKRLLDYYE DRGLVEVTP ISLLENFSN 
      NPPLQSLFL HKKVIERRL QEVIPYNDC LYKHLDEFK 
      FVVLLDTDE VIIPVEETW LQLIQTLEE NQPNRSSYM 
      ARNVYFLDN NLHQHEWFK DVPQHMHML QHVIRARNY 
      TKPGHFVKG FHDTRKVEA LHNHFPLKC LKGCDHVSI 
      NVSLAHLQH YRTGCARGV SSKTCDQMR DDTVMDTRI 
      WNFKQELIR RVDRVSTNL GLL
    CDS sequence
    • >TC015154 RA
      atgtcgaag aaactgtgc ttgttgttc ctaataacc 
      cttggtatc atctgcact ttcgtcatc tccaagaac 
      ctaacttcg gaccaaacc cccaccaaa caccacacc 
      ttccgccaa atcctccaa gcccccagc gaccccctt 
      gacaaaccc ttctccctc ccgcccgcc aaaaacacc 
      accggcaac atccccagc gtgttcttc acccccaag 
      gcgccaaaa ccaaccaaa ctcaacaca acgtgtgcg 
      aaattcccc gacttgttc gacctccaa ttccggaac 
      gaccactgg cagctccag aaaacatcc accgggtcg 
      ttgttcctc ttcaacgcg tattttgac cgtcgtggg 
      ggcgacttc gtgcgcatt atcaccatg ttcacccaa 
      cacccgcca gaaacccaa ttctggtgc caattctgg 
      taccagaac tccacaaaa ccggtgatt gtggaaagt 
      gaggcccct ttttggctg ttttcccgc aaatggggg 
      gccgaccgg ggccagttc gtgcaccct tacttgatc 
      aagtgtccc gtgaaaggg gcccctttc ggggtgtcc 
      ctcgtggaa aaaccgtgt gataacgcg agcaatgtt 
      ttgcaaatt tttggcaaa gaaacagtg gtgaaaaaa 
      aatttcgtg gtgtgtgtc aaaggtttg aatttcccg 
      tttgaagac aagtcgcta cgtttggtc gaatggatc 
      gaacttttg caaatactg ggggttgaa aaaatcaac 
      ttttacgag tttcgggtg caccccaac acgaaacgg 
      ttgctggat tattacgag gatcggggg ctggttgag 
      gtgacgccc atttcgctg ttggagaac ttctccaat 
      aatcccccc cttcaatca ctgttcctg cacaagaag 
      gtgattgag cggcgcttg caggaggtg atcccctac 
      aatgattgt ctctacaag cacttggac gagttcaag 
      tttgtcgtg cttttggac actgatgag gtgataatt 
      ccggttgaa gaaacgtgg cttcagttg atccagact 
      ttggaggaa aatcaaccg aatcggagc tcgtatatg 
      gcgcgaaat gtctatttc ctggacaat aacttgcac 
      caacatgag tggtttaag gacgtgccg cagcacatg 
      cacatgttg caacacgtc atacgtgcg agaaattac 
      accaagccg gggcatttc gttaagggg tttcatgat 
      acgcggaaa gttgaggct ttgcataac cattttccg 
      ctcaagtgt ttgaaaggt tgcgaccac gtttcgatc 
      aatgtcagt ctggcgcat cttcagcat tatcggact 
      gggtgtgct aggggggtt agttcaaag acttgtgac 
      cagatgagg gatgataca gtgatggat acgagaatt 
      tggaatttt aagcaagag ttgataagg agggtggat 
      agggttagc acgaattta gggttgttg tga
    mRNA sequence
    • >TC015154 RA
      ctagttcag tcacatcac acatccaaa catgtcgaa 
      gaaactgtg cttgttgtt cctaataac ccttggtat 
      catctgcac tttcgtcat ctccaagaa cctaacttc 
      ggaccaaac ccccaccaa acaccacac cttccgcca 
      aatcctcca agcccccag cgaccccct tgacaaacc 
      cttctccct cccgcccgc caaaaacac caccggcaa 
      catccccag cgtgttctt cacccccaa ggcgccaaa 
      accaaccaa actcaacac aacgtgtgc gaaattccc 
      cgacttgtt cgacctcca attccggaa cgaccactg 
      gcagctcca gaaaacatc caccgggtc gttgttcct 
      cttcaacgc gtattttga ccgtcgtgg gggcgactt 
      cgtgcgcat tatcaccat gttcaccca acacccgcc 
      agaaaccca attctggtg ccaattctg gtaccagaa 
      ctccacaaa accggtgat tgtggaaag tgaggcccc 
      tttttggct gttttcccg caaatgggg ggccgaccg 
      gggccagtt cgtgcaccc ttacttgat caagtgtcc 
      cgtgaaagg ggccccttt cggggtgtc cctcgtgga 
      aaaaccgtg tgataacgc gagcaatgt tttgcaaat 
      ttttggcaa agaaacagt ggtgaaaaa aaatttcgt 
      ggtgtgtgt caaaggttt gaatttccc gtttgaaga 
      caagtcgct acgtttggt cgaatggat cgaactttt 
      gcaaatact gggggttga aaaaatcaa cttttacga 
      gtttcgggt gcaccccaa cacgaaacg gttgctgga 
      ttattacga ggatcgggg gctggttga ggtgacgcc 
      catttcgct gttggagaa cttctccaa taatccccc 
      ccttcaatc actgttcct gcacaagaa ggtgattga 
      gcggcgctt gcaggaggt gatccccta caatgattg 
      tctctacaa gcacttgga cgagttcaa gtttgtcgt 
      gcttttgga cactgatga ggtgataat tccggttga 
      agaaacgtg gcttcagtt gatccagac tttggagga 
      aaatcaacc gaatcggag ctcgtatat ggcgcgaaa 
      tgtctattt cctggacaa taacttgca ccaacatga 
      gtggtttaa ggacgtgcc gcagcacat gcacatgtt 
      gcaacacgt catacgtgc gagaaatta caccaagcc 
      ggggcattt cgttaaggg gtttcatga tacgcggaa 
      agttgaggc tttgcataa ccattttcc gctcaagtg 
      tttgaaagg ttgcgacca cgtttcgat caatgtcag 
      tctggcgca tcttcagca ttatcggac tgggtgtgc 
      taggggggt tagttcaaa gacttgtga ccagatgag 
      ggatgatac agtgatgga tacgagaat ttggaattt 
      taagcaaga gttgataag gagggtgga tagggttag 
      cacgaattt agggttgtt gtgaagtta ctgtgatag 
      tagtttagt tatttattt tgtatgcaa ataaagaaa 
      aaggactgt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY