Status
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Gene information
General information
TC015151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2347466..2349976
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015151
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05763

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015151 RA
      MKILLLIFL TSLIQNSSQ VCRVRRPRP HIIVIMGDD 
      MGHNDIGLR TNQIPTPNI DALGYNGVV LDRYYVQNA 
      CTPSRAAFL TGNYPIRSA MQGLPIVAG ENRSLPLNM 
      PLMPQHLKN LGYRTHIVG KWHLGSAYR SSTPTEKGF 
      DSHFGYWNG FTGYYDYFT DFNSTAIEG FDLHDRFET 
      ERGYQGQYA TRVFTERAL DIIEGHNTT RPLFLLMTH 
      LAAHAGRDG TELGVPNEV EAQRTYSYI QDPRRRLYA 
      EIVAELDRS IGQVVRKLS ERQMLENSI ILFFSDNGA 
      PTVGPYTNS GSNWPLRGI KLTNFEGGI RGTATIFSP 
      LLKKRGYVN KELIHVSDW LPTFYAAAG GNLADLGPI 
      DGVNQWPTL SLDTPSPRS EILVNINEQ DNTTSIITD 
      NGRFKLVTG AFEGGTYDG YFGDSGRSP DTPPYDPFA 
      VLQSETNIA IQELTQTPI TRQQIRVTR AQIDLSWCR 
      NDSFRPPLN CSQPCLFDL ENDPCETTN IIEENPEIA 
      ERLLRRIAD FFEVLVPQG DSTIDPNAN PQRYNNTWC 
      TWLDDDWCV KTPNNTRLF
    CDS sequence
    • >TC015151 RA
      atgaaaata cttctcctt attttccta acttcctta 
      atacaaaac tcgagtcaa gtatgccgc gtacgccga 
      ccccgaccc cacattatc gtgataatg ggcgacgat 
      atgggacat aacgatatt ggtcttcgg acgaaccaa 
      atcccgacc cctaacatc gacgctttg gggtataat 
      ggggtagtt ttggaccgg tactacgtc cagaatgct 
      tgtacccca tcacgtgcc gccttcctc accggaaac 
      taccccatc cggtctgca atgcaaggt ttgcccata 
      gttgcaggc gaaaaccgc tcactaccc cttaatatg 
      cccctaatg ccccaacat ttgaaaaat ttggggtac 
      cgcacccat attgtcgga aaatggcat ttggggtcg 
      gcgtaccgg agtagtacc ccaactgag aaggggttt 
      gattcgcat tttgggtac tggaacggg tttacgggg 
      tactatgac tattttacg gattttaac agcacggcg 
      attgaaggg tttgattta cacgaccgg tttgagact 
      gaaaggggg taccaaggg caatatgcc actagggtt 
      tttaccgaa agggcatta gatattata gaagggcat 
      aataccacc agacccctt ttcctactt atgacccat 
      ttggcggcc catgctggg cgtgatggt actgagtta 
      ggggtcccc aatgaggtt gaggcccaa aggacgtac 
      tcctacatc caggacccc cgacgaagg ctttacgcc 
      gaaattgta gcagagttg gaccgatcg atcggacaa 
      gttgttaga aaattatca gaacgtcag atgcttgaa 
      aattcaatt attttattt ttctcggat aatggggcc 
      cccactgtg gggccctac acgaattca gggtcgaat 
      tggcccctg agagggatc aaattgacc aattttgaa 
      gggggtatt aggggcaca gccactatt tttagcccc 
      ttgttgaaa aaaaggggt tatgttaat aaggaattg 
      atacatgtt tctgactgg ttgcccact ttttacgca 
      gcggcaggt ggaaacttg gcggacttg ggccccatt 
      gacggggtc aaccaatgg cccaccctc tccctggac 
      accccctcc ccccgttcg gagatcctc gtcaacatc 
      aacgaacaa gacaacacc acgtccatc atcaccgac 
      aacggccga tttaaactc gtcacaggg gctttcgaa 
      ggtggcaca tacgatgga tatttcggg gacagtggg 
      cgcagccct gatacaccc ccttatgac ccttttgcc 
      gttctccaa agtgagaca aacattgcc attcaggaa 
      ctaacacaa acaccaatc acgcgacaa caaattcgg 
      gtcacaagg gctcagatc gacctgtct tggtgtcga 
      aatgactct ttcagaccc ccattgaat tgctcacaa 
      ccgtgtctt tttgatttg gaaaatgac ccgtgcgag 
      actactaat ataattgag gaaaatccc gaaatagca 
      gagaggctt ttgaggcga attgccgac ttttttgag 
      gttttggtg cctcaagga gactccacg attgatccg 
      aatgccaat ccgcaacgt tataataac acgtggtgc 
      acgtggctc gacgatgat tggtgtgtc aaaacgcca 
      aacaacacc aggttgttt tga
    mRNA sequence
    • >TC015151 RA
      tgacgttta gataactat ttaaaaaaa ttataaaaa 
      aatatcgct tagtcgcct caccgtctt tagcacagt 
      taacatgaa aatacttct ccttatttt cctaacttc 
      cttaataca aaactcgag tcaagtatg ccgcgtacg 
      ccgaccccg accccacat tatcgtgat aatgggcga 
      cgatatggg acataacga tattggtct tcggacgaa 
      ccaaatccc gacccctaa catcgacgc tttggggta 
      taatggggt agttttgga ccggtacta cgtccagaa 
      tgcttgtac cccatcacg tgccgcctt cctcaccgg 
      aaactaccc catccggtc tgcaatgca aggtttgcc 
      catagttgc aggcgaaaa ccgctcact accccttaa 
      tatgcccct aatgcccca acatttgaa aaatttggg 
      gtaccgcac ccatattgt cggaaaatg gcatttggg 
      gtcggcgta ccggagtag taccccaac tgagaaggg 
      gtttgattc gcattttgg gtactggaa cgggtttac 
      ggggtacta tgactattt tacggattt taacagcac 
      ggcgattga agggtttga tttacacga ccggtttga 
      gactgaaag ggggtacca agggcaata tgccactag 
      ggtttttac cgaaagggc attagatat tatagaagg 
      gcataatac caccagacc ccttttcct acttatgac 
      ccatttggc ggcccatgc tgggcgtga tggtactga 
      gttaggggt ccccaatga ggttgaggc ccaaaggac 
      gtactccta catccagga cccccgacg aaggcttta 
      cgccgaaat tgtagcaga gttggaccg atcgatcgg 
      acaagttgt tagaaaatt atcagaacg tcagatgct 
      tgaaaattc aattatttt atttttctc ggataatgg 
      ggcccccac tgtggggcc ctacacgaa ttcagggtc 
      gaattggcc cctgagagg gatcaaatt gaccaattt 
      tgaaggggg tattagggg cacagccac tatttttag 
      ccccttgtt gaaaaaaag gggttatgt taataagga 
      attgataca tgtttctga ctggttgcc cacttttta 
      cgcagcggc aggtggaaa cttggcgga cttgggccc 
      cattgacgg ggtcaacca atggcccac cctctccct 
      ggacacccc ctccccccg ttcggagat cctcgtcaa 
      catcaacga acaagacaa caccacgtc catcatcac 
      cgacaacgg ccgatttaa actcgtcac aggggcttt 
      cgaaggtgg cacatacga tggatattt cggggacag 
      tgggcgcag ccctgatac accccctta tgacccttt 
      tgccgttct ccaaagtga gacaaacat tgccattca 
      ggaactaac acaaacacc aatcacgcg acaacaaat 
      tcgggtcac aagggctca gatcgacct gtcttggtg 
      tcgaaatga ctctttcag acccccatt gaattgctc 
      acaaccgtg tctttttga tttggaaaa tgacccgtg 
      cgagactac taatataat tgaggaaaa tcccgaaat 
      agcagagag gcttttgag gcgaattgc cgacttttt 
      tgaggtttt ggtgcctca aggagactc cacgattga 
      tccgaatgc caatccgca acgttataa taacacgtg 
      gtgcacgtg gctcgacga tgattggtg tgtcaaaac 
      gccaaacaa caccaggtt gttttgagg aaaataaat 
      ttttgtgaa caaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY