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Gene information
General information
TC015148 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2366257..2370216
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015148
      iBeetle screen iB_05761

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015148 RA
        MAEENNLPE DISNLEDEN VTEEEQQDN HPPAPKRAK 
        VVWQETLIA LVAAQQKQL SEMTEITRH SKIATGPTP 
        STSATGEGS AQALANTSF RLSEFNPEN SDYAIEEWL 
        DIATKLKAE LHIGDVLMI AKAGEALKG SAHRYYCDW 
        RPVHRTWDE FCKDLIVAF PDRETPGAR AFTAATLRS 
        RDCESLSDY GIRKLRSIN RFHRDLPWN TILSMVEYG 
        LDHGEAQAT IRMHQPTGD RELLKILSE FDARRRKQR 
        VMQNQSSRS TDVSSMPRR REKFVKGSC FRCGQSGHH 
        KNNCNVDID KDTGTKVAV ETKDSPPTC THCKKMGHT 
        EPNCWLKHG RPKKAFVVK K
      CDS sequence
      • >TC015148 RA
        atggcggag gaaaacaat cttcctgaa gacatctcg 
        aatcttgaa gacgagaat gtgacagaa gaagaacaa 
        caagacaat catcccccc gccccaaaa cgagccaag 
        gttgtgtgg caggaaacg ctaattgcg cttgtagct 
        gctcaacaa aaacagtta tctgagatg accgaaata 
        acacgtcat tccaagatt gcaactggg ccaacgcct 
        agcacgtca gcaaccgga gaaggttct gcgcaagct 
        ttagcaaac acatctttc cgcttgtcg gaatttaat 
        cccgaaaat agcgattac gcaatcgag gaatggttg 
        gacatcgca actaaactt aaggcagaa ttacatatt 
        ggggatgtc ctcatgatt gccaaagcg ggcgaagct 
        cttaagggg agtgcccat cgatactat tgtgactgg 
        agaccagta catcgcacc tgggatgaa ttttgcaaa 
        gaccttata gttgcgttc ccagatcgc gaaacacca 
        ggtgcccga gcatttact gctgctact ctccgcagt 
        cgcgattgt gagtcactt agcgactac ggaatccga 
        aagcttcga tccatcaat cgattccat cgcgacttg 
        ccttggaac accatcctt agtatggta gagtatggc 
        ttggaccac ggagaagct caggctacc atccgcatg 
        catcaaccc actggtgat cgggagctc ctgaagatc 
        ttgagcgag tttgatgct cgccgcagg aaacagcga 
        gtcatgcaa aaccagagt tctcggtcg actgatgtg 
        tctagtatg ccgcgacgt cgtgaaaaa tttgtgaaa 
        ggatcttgc tttagatgt ggtcaaagt ggacaccat 
        aaaaacaat tgtaatgtg gacattgac aaagatact 
        ggtactaag gtggcagta gagacaaaa gatagccca 
        ccaacgtgt acccactgt aagaagatg ggacacacg 
        gagccaaat tgttggttg aaacacggc aggccgaag 
        aaagcgttt gtggtgaaa aagtga
      mRNA sequence
      • >TC015148 RA
        tcgacaccc gaagagacg tttctaaca gacgacgtc 
        ggtttcgtg tgtgagtgt gtgcgtgcg agtgtgtca 
        atgtgaccc agagatgaa tctgcaaca ggtacggtg 
        acggccttg ccccaggtg tctaaatgc gcccattat 
        gtaaaaaac gcgggggta ccacaaggg tctgctaag 
        ggaccaatg ttgtgtttt caggtcggt ttgtaaaaa 
        tcggttacg aaatgatgc ccaggaatt catcgccga 
        ttatagaaa aggaaatta tctgtactt ataagttta 
        agacaaagg acaatggcg gaggaaaac aatcttcct 
        gaagacatc tcgaatctt gaagacgag aatgtgaca 
        gaagaagaa caacaagac aatcatccc cccgcccca 
        aaacgagcc aaggttgtg tggcaggaa acgctaatt 
        gcgcttgta gctgctcaa caaaaacag ttatctgag 
        atgaccgaa ataacacgt cattccaag attgcaact 
        gggccaacg cctagcacg tcagcaacc ggagaaggt 
        tctgcgcaa gctttagca aacacatct ttccgcttg 
        tcggaattt aatcccgaa aatagcgat tacgcaatc 
        gaggaatgg ttggacatc gcaactaaa cttaaggca 
        gaattacat attggggat gtcctcatg attgccaaa 
        gcgggcgaa gctcttaag gggagtgcc catcgatac 
        tattgtgac tggagacca gtacatcgc acctgggat 
        gaattttgc aaagacctt atagttgcg ttcccagat 
        cgcgaaaca ccaggtgcc cgagcattt actgctgct 
        actctccgc agtcgcgat tgtgagtca cttagcgac 
        tacggaatc cgaaagctt cgatccatc aatcgattc 
        catcgcgac ttgccttgg aacaccatc cttagtatg 
        gtagagtat ggcttggac cacggagaa gctcaggct 
        accatccgc atgcatcaa cccactggt gatcgggag 
        ctcctgaag atcttgagc gagtttgat gctcgccgc 
        aggaaacag cgagtcatg caaaaccag agttctcgg 
        tcgactgat gtgtctagt atgccgcga cgtcgtgaa 
        aaatttgtg aaaggatct tgctttaga tgtggtcaa 
        agtggacac cataaaaac aattgtaat gtggacatt 
        gacaaagat actggtact aaggtggca gtagagaca 
        aaagatagc ccaccaacg tgtacccac tgtaagaag 
        atgggacac acggagcca aattgttgg ttgaaacac 
        ggcaggccg aagaaagcg tttgtggtg aaaaagtga 
        cgccactta aatacaacg ccaatggct tc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY