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Gene information
General information
TC015145 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2403615..2408286
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015145
    iBeetle screen iB_05760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015145 RA
      MRTVCCKSS DSDDCPRDV HSCSCIPSC 
      CQTPCPCQA CCEESCCED RCFKRIHCR 
      PRSPKPLNY PPRPPKCTC NKCCPPPPM 
      KRCPDPCCS RKKSCRPPP CPPTPRCPP 
      PPRCPSPQR CRSPCPPPS NNCCKNKMI 
      YFRPRCPSP SCGRSRSCK PRPCKRCCS 
      CEDRCKRCG EKVFAAEKV LTSHGSYHL 
      GCFSCYCCC KSLCVKTMY EACGEIYCR 
      QCYNNYFGI SSYGYCGNV C
    • >TC015145 RB
      MSCCARCGQ GICSTAGKI ALNKVWHKG CFCCYNCNR 
      PLNVRSAKI YYGEVFCAC CCPPKEVCR VNSTPSFNY 
      CERMRTVCC KSSDSDDCP RDVHSCSCI PSCCQTPCP 
      CQACCEESC CEDRCFKRI HCRPRSPKP LNYPPRPPK 
      CTCNKCCPP PPMKRCPDP CCSRKKSCR PPPCPPTPR 
      CPPPPRCPS PQRCRSPCP PPSNNCCKN KMIYFRPRC 
      PSPSCGRSR SCKPRPCKR CCSCEDRCK RCGEKVFAA 
      EKVLTSHGS YHLGCFSCY CCCKSLCVK TMYEACGEI 
      YCRQCYNNY FGISSYGYC GNVC
    CDS sequence
    • >TC015145 RA
      atgaggacg gtttgctgc aagtcgagc gactctgat 
      gactgcccc cgcgacgtc cactcctgc agctgcatc 
      ccctcctgc tgccaaact ccctgtcct tgtcaagcc 
      tgctgcgaa gaatcctgc tgtgaagat cgctgtttc 
      aagcgcatc cactgccgt ccccgctcc cccaaaccc 
      ctcaattat cctccacgt cctcccaaa tgcacttgc 
      aacaaatgt tgtcctcct cctccaatg aaacgttgt 
      ccggatcct tgctgttcc aggaaaaaa tcctgtcga 
      cctcctccc tgccctccc actccacgt tgccctcca 
      cctccacgc tgcccttcc ccacaacgt tgtcgatcg 
      ccatgtcct cctccctcc aataattgt tgcaagaac 
      aaaatgatt tattttcga cctcgatgt ccgagtcct 
      tcatgcgga cgtagtcga agttgtaaa ccacgtcct 
      tgcaagaga tgttgttcg tgtgaagat agatgtaag 
      agatgtgga gagaaagtt tttgcagct gagaaggtt 
      ttaacgtcg catgggtcc tatcatttg gggtgtttt 
      tcttgctat tgttgttgc aagtcgttg tgtgtgaag 
      acgatgtat gaggcgtgc ggggagatt tattgcaga 
      cagtgctac aataactac ttcggcatc agcagttac 
      ggctactgc ggcaacgtc tgctga
    • >TC015145 RB
      atgtcttgc tgcgcacgg tgcggccaa ggcatctgt 
      tcgactgct ggtaaaatc gcgcttaac aaagtctgg 
      cataagggc tgtttctgt tgctataac tgtaatcga 
      ccgttgaat gtgcgctcg gccaaaatc tactacgga 
      gaggttttc tgcgcctgc tgctgtcct ccgaaggag 
      gtgtgcaga gttaactcg acaccgagc ttcaattac 
      tgcgagagg atgaggacg gtttgctgc aagtcgagc 
      gactctgat gactgcccc cgcgacgtc cactcctgc 
      agctgcatc ccctcctgc tgccaaact ccctgtcct 
      tgtcaagcc tgctgcgaa gaatcctgc tgtgaagat 
      cgctgtttc aagcgcatc cactgccgt ccccgctcc 
      cccaaaccc ctcaattat cctccacgt cctcccaaa 
      tgcacttgc aacaaatgt tgtcctcct cctccaatg 
      aaacgttgt ccggatcct tgctgttcc aggaaaaaa 
      tcctgtcga cctcctccc tgccctccc actccacgt 
      tgccctcca cctccacgc tgcccttcc ccacaacgt 
      tgtcgatcg ccatgtcct cctccctcc aataattgt 
      tgcaagaac aaaatgatt tattttcga cctcgatgt 
      ccgagtcct tcatgcgga cgtagtcga agttgtaaa 
      ccacgtcct tgcaagaga tgttgttcg tgtgaagat 
      agatgtaag agatgtgga gagaaagtt tttgcagct 
      gagaaggtt ttaacgtcg catgggtcc tatcatttg 
      gggtgtttt tcttgctat tgttgttgc aagtcgttg 
      tgtgtgaag acgatgtat gaggcgtgc ggggagatt 
      tattgcaga cagtgctac aataactac ttcggcatc 
      agcagttac ggctactgc ggcaacgtc tgctga
    mRNA sequence
    • >TC015145 RA
      ttcctgtgt ttcccccgc aataagggt atgtgttaa 
      catgtcttg ctgcgcacg gtgcggcca aggcatctg 
      ttcgactgc tggtaaaat cgcgcttaa caaagtctg 
      gcataaggg ctgtttctg ttgcttcta cagataact 
      gtaatcgac cgttgaatg tgcgctcgg ccaaaatct 
      actacggag aggttttct gcgcctgct gctgtcctc 
      cgaaggagg tgtgcagag ttaactcga caccgagct 
      tcaattact gcgagagga tgaggacgg tttgctgca 
      agtcgagcg actctgatg actgccccc gcgacgtcc 
      actcctgca gctgcatcc cctcctgct gccaaactc 
      cctgtcctt gtcaagcct gctgcgaag aatcctgct 
      gtgaagatc gctgtttca agcgcatcc actgccgtc 
      cccgctccc ccaaacccc tcaattatc ctccacgtc 
      ctcccaaat gcacttgca acaaatgtt gtcctcctc 
      ctccaatga aacgttgtc cggatcctt gctgttcca 
      ggaaaaaat cctgtcgac ctcctccct gccctccca 
      ctccacgtt gccctccac ctccacgct gcccttccc 
      cacaacgtt gtcgatcgc catgtcctc ctccctcca 
      ataattgtt gcaagaaca aaatgattt attttcgac 
      ctcgatgtc cgagtcctt catgcggac gtagtcgaa 
      gttgtaaac cacgtcctt gcaagagat gttgttcgt 
      gtgaagata gatgtaaga gatgtggag agaaagttt 
      ttgcagctg agaaggttt taacgtcgc atgggtcct 
      atcatttgg ggtgttttt cttgctatt gttgttgca 
      agtcgttgt gtgtgaaga cgatgtatg aggcgtgcg 
      gggagattt attgcagac agtgctaca ataactact 
      tcggcatca gcagttacg gctactgcg gcaacgtct 
      gctgacgtc atttcacca ccaataaaa taacaaaaa 
      gcaa
    • >TC015145 RB
      ttcctgtgt ttcccccgc aataagggt atgtgttaa 
      catgtcttg ctgcgcacg gtgcggcca aggcatctg 
      ttcgactgc tggtaaaat cgcgcttaa caaagtctg 
      gcataaggg ctgtttctg ttgctataa ctgtaatcg 
      accgttgaa tgtgcgctc ggccaaaat ctactacgg 
      agaggtttt ctgcgcctg ctgctgtcc tccgaagga 
      ggtgtgcag agttaactc gacaccgag cttcaatta 
      ctgcgagag gatgaggac ggtttgctg caagtcgag 
      cgactctga tgactgccc ccgcgacgt ccactcctg 
      cagctgcat cccctcctg ctgccaaac tccctgtcc 
      ttgtcaagc ctgctgcga agaatcctg ctgtgaaga 
      tcgctgttt caagcgcat ccactgccg tccccgctc 
      ccccaaacc cctcaatta tcctccacg tcctcccaa 
      atgcacttg caacaaatg ttgtcctcc tcctccaat 
      gaaacgttg tccggatcc ttgctgttc caggaaaaa 
      atcctgtcg acctcctcc ctgccctcc cactccacg 
      ttgccctcc acctccacg ctgcccttc cccacaacg 
      ttgtcgatc gccatgtcc tcctccctc caataattg 
      ttgcaagaa caaaatgat ttattttcg acctcgatg 
      tccgagtcc ttcatgcgg acgtagtcg aagttgtaa 
      accacgtcc ttgcaagag atgttgttc gtgtgaaga 
      tagatgtaa gagatgtgg agagaaagt ttttgcagc 
      tgagaaggt tttaacgtc gcatgggtc ctatcattt 
      ggggtgttt ttcttgcta ttgttgttg caagtcgtt 
      gtgtgtgaa gacgatgta tgaggcgtg cggggagat 
      ttattgcag acagtgcta caataacta cttcggcat 
      cagcagtta cggctactg cggcaacgt ctgctgacg 
      tcatttcac caccaataa aataacaaa aagcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY