General information | |||
TC033376
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 2481372..2489365 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC033376 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05759 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Comment: Abdomens seem to be the most sensitive. |
Comment: Only one larva shows true muscle phenotype. Other cases seem to be obscured by segmentation defects. |
remarks: muscle pattern potentially obscured by segmentation defect,
legs slightly shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC033376 RA
MALKKVKGN IERMFDKNL TDLVRGIRN NKENEAKYI SQCMEDIKQ ELRQDNLSV KSNAIAKLT YLQMLGYDI SWAGFNIIE VMSSSKFTL KRIGYLAAS QSFHCDSEL LMLTTNMIR KELNSQNQY ESGLALTSL SCYISTDLA RDLSNDILT LLSSTKPYI RKKAVLMMY KVFLKYPEA LRPAFPKLK EKLEDPDPG VQSAAVNVV CELARKNPK NYLSLAPVF FKLMTTSTN NWMLIKIIK LFGALTPLE PRLGQKLIE PLTNLIHST SAMSLLYEC INTVIAVLI TISSGMPNH AASIQLCVQ KLRILIEDS DQNLKYLGL LAMSKILKT HPKSVQAHK DLILQCLED KDESIRLRA LDLLYGMVS KKNLMEIVK KLMVHMDKA EGTIYRDEL LNKIILICS QNNYQFVTN FEWYVTVLV ELAQMEFGS KQGHVIGAQ LMDVCIRVQ AIRPFAVSE MAQLLDVYS STSQFTIMQ EVLYAAAWL CGEFASELK EPEKTLRAM LKYKSLPQH IEAVFIHNI IKLFAHVMR GYEEEGQYG EILGLCDEI CVKLGESLK SGELEVQER SSTSLIIVQ IVKEEIEKV MGGTGEGEN SVSGDLLAL FTGELNPVA PKAQKKVPI PEGLDLDAW INEPPEDSD SSSDHEVFD NLFIKTEKS DYGNSVRYQ PEPTEEEIR KSREARKYE QQNNPHYLK TSDVDKNAN DSNIDNIPV AEIDLNIPL KVVGQKRSD KYLNMNASR KKTKKKNKK KKHKNESSS DEENANEGP VVEVKRDLE LPEGATLSD SDSSHGHVD DPHRALNID LELPPVYET PVKAVEAKE EEKKVKKSK KNKGEKEEK EKKKKHHDK GAKKAKKEK KEKEGKSKK KKPTSGYEE ALGISTPSK EFQ
- >TC033376 RA
atggcctta aagaaggtt aaagggaac attgaacgc atgttcgac aagaatttg accgatttg gtccgcggg atccgcaat aataaggag aatgaggcg aagtacatc tcgcaatgc atggaggat atcaagcag gagctgcga caggataat ctctcagtt aagagtaac gcaatcgcg aaattgact tatttgcaa atgctgggg tacgatatt tcgtgggcc ggctttaac ataatcgaa gtgatgagt tcgagtaaa tttacgctg aaacggatc ggttatctc gcggctagt caatcgttt cattgtgat tcagagttg ctcatgttg accactaat atgattagg aaggagtta aactcgcaa aatcagtac gaatcgggg ctggccttg acgtccctc agctgttac atcagcact gacttggcc agagacctt tcaaacgat attttaact cttttaagt tccactaaa ccctacatt cggaaaaaa gccgttcta atgatgtat aaagtgttc ctcaaatat cccgaagcc ctacgtcct gcctttcct aaactaaaa gaaaagtta gaggacccc gatccgggg gttcagtcg gctgcagtc aatgttgtg tgtgaactg gcacgaaaa aaccccaaa aattatcta agtctggcc ccagttttt ttcaaatta atgaccaca agcaccaat aattggatg ctaattaaa ataatcaaa ctgttcggt gctttaacg cctcttgaa cctaggcta ggtcaaaag ttgattgag cctttgact aatctcata cacagcact tcggcaatg agtctctta tatgagtgt ataaatacg gttatagca gttttgata acaatatcg tcaggaatg cccaatcac gccgctagc atacaactc tgtgtccaa aaactgcgg attttgatt gaagattcc gaccagaat ttgaaatat ttgggcctt ttggcgatg tcaaaaatc ctcaaaacg cacccaaaa agcgtccaa gcccacaaa gacctaata ttgcaatgt ctagaggac aaagacgag tccatccga ctccgcgcc ttagacttg ctatacggc atggtctcg aaaaaaaat ctaatggaa atcgtcaaa aaactaatg gtccatatg gacaaagcc gaaggcacc atctaccga gacgaactt ttaaacaaa ataattcta atttgctcg caaaacaac taccaattc gtcacgaat ttcgaatgg tatgttact gttttggtc gagttggcg cagatggag ttcgggtcg aagcaaggg cacgtgatt ggggcgcaa ttaatggac gtttgtatc cgagttcaa gcgattcgt ccgttcgcc gtctccgaa atggcgcaa cttttggac gtttactcg tcaacttcg caatttacg ataatgcaa gaggtgttg tatgcggcg gcttggctg tgtggggag tttgcctcc gagttgaaa gagccagag aagacgctt cgagcaatg ttgaagtat aaaagtttg cctcagcac attgaggct gtttttata cacaatata atcaagttg tttgctcat gtaatgcga gggtatgag gaggagggg caatatggg gagattttg gggctgtgt gatgagatt tgtgtaaag ttgggggag agtttgaag tcaggggag ttggaggtg caggagagg tcaagtacg tcgcttatt atcgtgcag attgtgaag gaggagatt gagaaggtt atggggggg acgggagaa ggggagaat tcagtgtcg ggggattta ctcgctctg tttacgggg gagttgaat ccggtggcc cccaaagcc cagaagaag gtcccaata ccagaaggg ttagatttg gacgcttgg atcaacgaa ccgcccgaa gattcggac tcgtcaagc gatcacgag gtcttcgac aaccttttc atcaaaacg gaaaaatca gactatggc aattcggtt cggtaccaa ccggaaccg accgaagaa gaaatccgc aaaagtcgc gaagcccga aaatacgaa caacaaaac aatccgcac tatctcaaa acatcagac gttgataaa aatgcaaac gatagcaac attgataac atcccagtt gctgaaatt gatttgaac attccgttg aaagtcgtg ggccaaaaa cgttccgac aaatatttg aacatgaac gcgagccgg aagaagacg aaaaagaag aacaaaaag aagaaacac aagaacgaa agttcctca gacgaggaa aatgcgaat gaaggtcca gtagttgaa gttaaaagg gacttggag ttgcccgaa ggggcgact ttgtcggac tccgacagc agtcacggg cacgttgat gacccccat agggccctc aatatcgat ctagaactg cccccagtt tacgaaact ccagtcaag gcggtcgag gcgaaagag gaggaaaag aaagtgaaa aaatcgaaa aagaacaag ggcgagaag gaggagaag gagaagaag aagaagcac catgacaag ggcgcgaag aaagcgaag aaggagaag aaggagaaa gagggcaag tcgaagaag aagaagccg acgtctggc tacgaggaa gcgctaggt atttcaaca cccagcaag gaattccaa tga
- >TC033376 RA
cagttattc agttgcatt tagctagaa tagacgttc gcaatgttc gatagaaag ttagttttt gctctcgtt ttcgtcgtt tttgccact ttagcggtc gaaggcagg tatctacca acgaggtcc aacggggac aggatcgaa aaactccgc gagttgctc aaagacttg ttcgaaaat gaggtcgag aaagaagag taccaggcc gacgcgccc ccgcggtgg caccccgaa agcaagctc ttctacaaa cgcgaggcc cccgcacac tagaccacc cccacaacg acaaggcaa cggacacgg aacacgatt tttaaacat tcaatgtca taggtagac acattttaa actttattt tattaccga caagaacaa ttatttttg taaataagt ggccccctg attgacaat aacgccacg acccttgat aagcaatta tgtagtttt agggaattg tgtcaatta cttgacaat attatcatt ttagaattg attgagaga ccgtcccga gggaggttg tcggtaata aagctcttt attgcaatt tgtgaagca atattttga cgaaacttt taataagtg tgtcatttg taatatcct aaggtgtct tattaaagt gttacatgg aatatgtgc atgggaata aaaatatat gaacatcca atcgctttt tattattta caaactaat agttaagtt attgcgttg tctgctcct cccacggcc ggggccccc tgacctgct cccaattat cgatatcaa tcttcttga tcttctcgt actccgact cgagcgtga cttcgcccg gtttcttca tttcaatcc ccaatttct tcatttctt cgggcttaa cactggaga ctcgtgaaa actgatacc tcaactgtg caaattcac ggagcccga acgaacccc ccaaaacca caagaagaa acggcaaac catatttca ccgaacgtt tattcaaaa actcgttta ttttcgccc ccacgttat gcaacattt tttgaggtt atgtcacca catttggta aaaaacagt tttgggggt aacaaccgg tcggtgaac ctcccccag ggataaaat caccagttt tgatcaatt atacgactc gtcaagggc taggaacgc gtttattgt gtgttattt tgacgagcc gccactgtc atctgtcat taacttgtc agttgtcaa cgtggctac gaaaagtaa aaaactggg gcgaacgct cgagcgccc ctttaaaac atcctgtga tatgaaaga actgaaatt cgggcgatt ctgtgcgga actattgcc ccaattcga acaaactgg tcgggtggc tgtgtcgcc ccctgattt gatttagtc agtttttta cgcaaaatg gccttaaag aaggttaaa gggaacatt gaacgcatg ttcgacaag aatttgacc gatttggtc cgcgggatc cgcaataat aaggagaat gaggcgaag tacatctcg caatgcatg gaggatatc aagcaggag ctgcgacag gataatctc tcagttaag agtaacgca atcgcgaaa ttgacttat ttgcaaatg ctggggtac gatatttcg tgggccggc tttaacata atcgaagtg atgagttcg agtaaattt acgctgaaa cggatcggt tatctcgcg gctagtcaa tcgtttcat tgtgattca gagttgctc atgttgacc actaatatg attaggaag gagttaaac tcgcaaaat cagtacgaa tcggggctg gccttgacg tccctcagc tgttacatc agcactgac ttggccaga gacctttca aacgatatt ttaactctt ttaagttcc actaaaccc tacattcgg aaaaaagcc gttctaatg atgtataaa gtgttcctc aaatatccc gaagcccta cgtcctgcc tttcctaaa ctaaaagaa aagttagag gaccccgat ccgggggtt cagtcggct gcagtcaat gttgtgtgt gaactggca cgaaaaaac cccaaaaat tatctaagt ctggcccca gtttttttc aaattaatg accacaagc accaataat tggatgcta attaaaata atcaaactg ttcggtgct ttaacgcct cttgaacct aggctaggt caaaagttg attgagcct ttgactaat ctcatacac agcacttcg gcaatgagt ctcttatat gagtgtata aatacggtt atagcagtt ttgataaca atatcgtca ggaatgccc aatcacgcc gctagcata caactctgt gtccaaaaa ctgcggatt ttgattgaa gattccgac cagaatttg aaatatttg ggccttttg gcgatgtca aaaatcctc aaaacgcac ccaaaaagc gtccaagcc cacaaagac ctaatattg caatgtcta gaggacaaa gacgagtcc atccgactc cgcgcctta gacttgcta tacggcatg gtctcgaaa aaaaatcta atggaaatc gtcaaaaaa ctaatggtc catatggac aaagccgaa ggcaccatc taccgagac gaactttta aacaaaata attctaatt tgctcgcaa aacaactac caattcgtc acgaatttc gaatggtat gttactgtt ttggtcgag ttggcgcag atggagttc gggtcgaag caagggcac gtgattggg gcgcaatta atggacgtt tgtatccga gttcaagcg attcgtccg ttcgccgtc tccgaaatg gcgcaactt ttggacgtt tactcgtca acttcgcaa tttacgata atgcaagag gtgttgtat gcggcggct tggctgtgt ggggagttt gcctccgag ttgaaagag ccagagaag acgcttcga gcaatgttg aagtataaa agtttgcct cagcacatt gaggctgtt tttatacac aatataatc aagttgttt gctcatgta atgcgaggg tatgaggag gaggggcaa tatggggag attttgggg ctgtgtgat gagatttgt gtaaagttg ggggagagt ttgaagtca ggggagttg gaggtgcag gagaggtca agtacgtcg cttattatc gtgcagatt gtgaaggag gagattgag aaggttatg ggggggacg ggagaaggg gagaattca gtgtcgggg gatttactc gctctgttt acgggggag ttgaatccg gtggccccc aaagcccag aagaaggtc ccaatacca gaagggtta gatttggac gcttggatc aacgaaccg cccgaagat tcggactcg tcaagcgat cacgaggtc ttcgacaac cttttcatc aaaacggaa aaatcagac tatggcaat tcggttcgg taccaaccg gaaccgacc gaagaagaa atccgcaaa agtcgcgaa gcccgaaaa tacgaacaa caaaacaat ccgcactat ctcaaaaca tcagacgtt gataaaaat gcaaacgat agcaacatt gataacatc ccagttgct gaaattgat ttgaacatt ccgttgaaa gtcgtgggc caaaaacgt tccgacaaa tatttgaac atgaacgcg agccggaag aagacgaaa aagaagaac aaaaagaag aaacacaag aacgaaagt tcctcagac gaggaaaat gcgaatgaa ggtccagta gttgaagtt aaaagggac ttggagttg cccgaaggg gcgactttg tcggactcc gacagcagt cacgggcac gttgatgac ccccatagg gccctcaat atcgatcta gaactgccc ccagtttac gaaactcca gtcaaggcg gtcgaggcg aaagaggag gaaaagaaa gtgaaaaaa tcgaaaaag aacaagggc gagaaggag gagaaggag aagaagaag aagcaccat gacaagggc gcgaagaaa gcgaagaag gagaagaag gagaaagag ggcaagtcg aagaagaag aagccgacg tctggctac gaggaagcg ctaggtatt tcaacaccc agcaaggaa ttccaatga ttttctctt tatgtgcgc ctacaaatc attttataa tcattcaaa ttaatcatg ttattgtat tattgttta ttaaagaag cgataaaga t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
