Status
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Gene information
General information
TC033376
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015138 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2481372..2489365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033376
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05759

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Abdomens seem to be the most sensitive.
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Only one larva shows true muscle phenotype. Other cases seem to be obscured by segmentation defects.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     legs slightly shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033376 RA
      MALKKVKGN IERMFDKNL TDLVRGIRN NKENEAKYI 
SQCMEDIKQ ELRQDNLSV KSNAIAKLT YLQMLGYDI 
SWAGFNIIE VMSSSKFTL KRIGYLAAS QSFHCDSEL 
LMLTTNMIR KELNSQNQY ESGLALTSL SCYISTDLA 
RDLSNDILT LLSSTKPYI RKKAVLMMY KVFLKYPEA 
LRPAFPKLK EKLEDPDPG VQSAAVNVV CELARKNPK 
NYLSLAPVF FKLMTTSTN NWMLIKIIK LFGALTPLE 
PRLGQKLIE PLTNLIHST SAMSLLYEC INTVIAVLI 
TISSGMPNH AASIQLCVQ KLRILIEDS DQNLKYLGL 
LAMSKILKT HPKSVQAHK DLILQCLED KDESIRLRA 
LDLLYGMVS KKNLMEIVK KLMVHMDKA EGTIYRDEL 
LNKIILICS QNNYQFVTN FEWYVTVLV ELAQMEFGS 
KQGHVIGAQ LMDVCIRVQ AIRPFAVSE MAQLLDVYS 
STSQFTIMQ EVLYAAAWL CGEFASELK EPEKTLRAM 
LKYKSLPQH IEAVFIHNI IKLFAHVMR GYEEEGQYG 
EILGLCDEI CVKLGESLK SGELEVQER SSTSLIIVQ 
IVKEEIEKV MGGTGEGEN SVSGDLLAL FTGELNPVA 
PKAQKKVPI PEGLDLDAW INEPPEDSD SSSDHEVFD 
NLFIKTEKS DYGNSVRYQ PEPTEEEIR KSREARKYE 
QQNNPHYLK TSDVDKNAN DSNIDNIPV AEIDLNIPL 
KVVGQKRSD KYLNMNASR KKTKKKNKK KKHKNESSS 
DEENANEGP VVEVKRDLE LPEGATLSD SDSSHGHVD 
DPHRALNID LELPPVYET PVKAVEAKE EEKKVKKSK 
KNKGEKEEK EKKKKHHDK GAKKAKKEK KEKEGKSKK 
KKPTSGYEE ALGISTPSK EFQ
    CDS sequence
    • >TC033376 RA
      atggcctta aagaaggtt aaagggaac attgaacgc 
atgttcgac aagaatttg accgatttg gtccgcggg 
atccgcaat aataaggag aatgaggcg aagtacatc 
tcgcaatgc atggaggat atcaagcag gagctgcga 
caggataat ctctcagtt aagagtaac gcaatcgcg 
aaattgact tatttgcaa atgctgggg tacgatatt 
tcgtgggcc ggctttaac ataatcgaa gtgatgagt 
tcgagtaaa tttacgctg aaacggatc ggttatctc 
gcggctagt caatcgttt cattgtgat tcagagttg 
ctcatgttg accactaat atgattagg aaggagtta 
aactcgcaa aatcagtac gaatcgggg ctggccttg 
acgtccctc agctgttac atcagcact gacttggcc 
agagacctt tcaaacgat attttaact cttttaagt 
tccactaaa ccctacatt cggaaaaaa gccgttcta 
atgatgtat aaagtgttc ctcaaatat cccgaagcc 
ctacgtcct gcctttcct aaactaaaa gaaaagtta 
gaggacccc gatccgggg gttcagtcg gctgcagtc 
aatgttgtg tgtgaactg gcacgaaaa aaccccaaa 
aattatcta agtctggcc ccagttttt ttcaaatta 
atgaccaca agcaccaat aattggatg ctaattaaa 
ataatcaaa ctgttcggt gctttaacg cctcttgaa 
cctaggcta ggtcaaaag ttgattgag cctttgact 
aatctcata cacagcact tcggcaatg agtctctta 
tatgagtgt ataaatacg gttatagca gttttgata 
acaatatcg tcaggaatg cccaatcac gccgctagc 
atacaactc tgtgtccaa aaactgcgg attttgatt 
gaagattcc gaccagaat ttgaaatat ttgggcctt 
ttggcgatg tcaaaaatc ctcaaaacg cacccaaaa 
agcgtccaa gcccacaaa gacctaata ttgcaatgt 
ctagaggac aaagacgag tccatccga ctccgcgcc 
ttagacttg ctatacggc atggtctcg aaaaaaaat 
ctaatggaa atcgtcaaa aaactaatg gtccatatg 
gacaaagcc gaaggcacc atctaccga gacgaactt 
ttaaacaaa ataattcta atttgctcg caaaacaac 
taccaattc gtcacgaat ttcgaatgg tatgttact 
gttttggtc gagttggcg cagatggag ttcgggtcg 
aagcaaggg cacgtgatt ggggcgcaa ttaatggac 
gtttgtatc cgagttcaa gcgattcgt ccgttcgcc 
gtctccgaa atggcgcaa cttttggac gtttactcg 
tcaacttcg caatttacg ataatgcaa gaggtgttg 
tatgcggcg gcttggctg tgtggggag tttgcctcc 
gagttgaaa gagccagag aagacgctt cgagcaatg 
ttgaagtat aaaagtttg cctcagcac attgaggct 
gtttttata cacaatata atcaagttg tttgctcat 
gtaatgcga gggtatgag gaggagggg caatatggg 
gagattttg gggctgtgt gatgagatt tgtgtaaag 
ttgggggag agtttgaag tcaggggag ttggaggtg 
caggagagg tcaagtacg tcgcttatt atcgtgcag 
attgtgaag gaggagatt gagaaggtt atggggggg 
acgggagaa ggggagaat tcagtgtcg ggggattta 
ctcgctctg tttacgggg gagttgaat ccggtggcc 
cccaaagcc cagaagaag gtcccaata ccagaaggg 
ttagatttg gacgcttgg atcaacgaa ccgcccgaa 
gattcggac tcgtcaagc gatcacgag gtcttcgac 
aaccttttc atcaaaacg gaaaaatca gactatggc 
aattcggtt cggtaccaa ccggaaccg accgaagaa 
gaaatccgc aaaagtcgc gaagcccga aaatacgaa 
caacaaaac aatccgcac tatctcaaa acatcagac 
gttgataaa aatgcaaac gatagcaac attgataac 
atcccagtt gctgaaatt gatttgaac attccgttg 
aaagtcgtg ggccaaaaa cgttccgac aaatatttg 
aacatgaac gcgagccgg aagaagacg aaaaagaag 
aacaaaaag aagaaacac aagaacgaa agttcctca 
gacgaggaa aatgcgaat gaaggtcca gtagttgaa 
gttaaaagg gacttggag ttgcccgaa ggggcgact 
ttgtcggac tccgacagc agtcacggg cacgttgat 
gacccccat agggccctc aatatcgat ctagaactg 
cccccagtt tacgaaact ccagtcaag gcggtcgag 
gcgaaagag gaggaaaag aaagtgaaa aaatcgaaa 
aagaacaag ggcgagaag gaggagaag gagaagaag 
aagaagcac catgacaag ggcgcgaag aaagcgaag 
aaggagaag aaggagaaa gagggcaag tcgaagaag 
aagaagccg acgtctggc tacgaggaa gcgctaggt 
atttcaaca cccagcaag gaattccaa tga
    mRNA sequence
    • >TC033376 RA
      cagttattc agttgcatt tagctagaa tagacgttc 
gcaatgttc gatagaaag ttagttttt gctctcgtt 
ttcgtcgtt tttgccact ttagcggtc gaaggcagg 
tatctacca acgaggtcc aacggggac aggatcgaa 
aaactccgc gagttgctc aaagacttg ttcgaaaat 
gaggtcgag aaagaagag taccaggcc gacgcgccc 
ccgcggtgg caccccgaa agcaagctc ttctacaaa 
cgcgaggcc cccgcacac tagaccacc cccacaacg 
acaaggcaa cggacacgg aacacgatt tttaaacat 
tcaatgtca taggtagac acattttaa actttattt 
tattaccga caagaacaa ttatttttg taaataagt 
ggccccctg attgacaat aacgccacg acccttgat 
aagcaatta tgtagtttt agggaattg tgtcaatta 
cttgacaat attatcatt ttagaattg attgagaga 
ccgtcccga gggaggttg tcggtaata aagctcttt 
attgcaatt tgtgaagca atattttga cgaaacttt 
taataagtg tgtcatttg taatatcct aaggtgtct 
tattaaagt gttacatgg aatatgtgc atgggaata 
aaaatatat gaacatcca atcgctttt tattattta 
caaactaat agttaagtt attgcgttg tctgctcct 
cccacggcc ggggccccc tgacctgct cccaattat 
cgatatcaa tcttcttga tcttctcgt actccgact 
cgagcgtga cttcgcccg gtttcttca tttcaatcc 
ccaatttct tcatttctt cgggcttaa cactggaga 
ctcgtgaaa actgatacc tcaactgtg caaattcac 
ggagcccga acgaacccc ccaaaacca caagaagaa 
acggcaaac catatttca ccgaacgtt tattcaaaa 
actcgttta ttttcgccc ccacgttat gcaacattt 
tttgaggtt atgtcacca catttggta aaaaacagt 
tttgggggt aacaaccgg tcggtgaac ctcccccag 
ggataaaat caccagttt tgatcaatt atacgactc 
gtcaagggc taggaacgc gtttattgt gtgttattt 
tgacgagcc gccactgtc atctgtcat taacttgtc 
agttgtcaa cgtggctac gaaaagtaa aaaactggg 
gcgaacgct cgagcgccc ctttaaaac atcctgtga 
tatgaaaga actgaaatt cgggcgatt ctgtgcgga 
actattgcc ccaattcga acaaactgg tcgggtggc 
tgtgtcgcc ccctgattt gatttagtc agtttttta 
cgcaaaatg gccttaaag aaggttaaa gggaacatt 
gaacgcatg ttcgacaag aatttgacc gatttggtc 
cgcgggatc cgcaataat aaggagaat gaggcgaag 
tacatctcg caatgcatg gaggatatc aagcaggag 
ctgcgacag gataatctc tcagttaag agtaacgca 
atcgcgaaa ttgacttat ttgcaaatg ctggggtac 
gatatttcg tgggccggc tttaacata atcgaagtg 
atgagttcg agtaaattt acgctgaaa cggatcggt 
tatctcgcg gctagtcaa tcgtttcat tgtgattca 
gagttgctc atgttgacc actaatatg attaggaag 
gagttaaac tcgcaaaat cagtacgaa tcggggctg 
gccttgacg tccctcagc tgttacatc agcactgac 
ttggccaga gacctttca aacgatatt ttaactctt 
ttaagttcc actaaaccc tacattcgg aaaaaagcc 
gttctaatg atgtataaa gtgttcctc aaatatccc 
gaagcccta cgtcctgcc tttcctaaa ctaaaagaa 
aagttagag gaccccgat ccgggggtt cagtcggct 
gcagtcaat gttgtgtgt gaactggca cgaaaaaac 
cccaaaaat tatctaagt ctggcccca gtttttttc 
aaattaatg accacaagc accaataat tggatgcta 
attaaaata atcaaactg ttcggtgct ttaacgcct 
cttgaacct aggctaggt caaaagttg attgagcct 
ttgactaat ctcatacac agcacttcg gcaatgagt 
ctcttatat gagtgtata aatacggtt atagcagtt 
ttgataaca atatcgtca ggaatgccc aatcacgcc 
gctagcata caactctgt gtccaaaaa ctgcggatt 
ttgattgaa gattccgac cagaatttg aaatatttg 
ggccttttg gcgatgtca aaaatcctc aaaacgcac 
ccaaaaagc gtccaagcc cacaaagac ctaatattg 
caatgtcta gaggacaaa gacgagtcc atccgactc 
cgcgcctta gacttgcta tacggcatg gtctcgaaa 
aaaaatcta atggaaatc gtcaaaaaa ctaatggtc 
catatggac aaagccgaa ggcaccatc taccgagac 
gaactttta aacaaaata attctaatt tgctcgcaa 
aacaactac caattcgtc acgaatttc gaatggtat 
gttactgtt ttggtcgag ttggcgcag atggagttc 
gggtcgaag caagggcac gtgattggg gcgcaatta 
atggacgtt tgtatccga gttcaagcg attcgtccg 
ttcgccgtc tccgaaatg gcgcaactt ttggacgtt 
tactcgtca acttcgcaa tttacgata atgcaagag 
gtgttgtat gcggcggct tggctgtgt ggggagttt 
gcctccgag ttgaaagag ccagagaag acgcttcga 
gcaatgttg aagtataaa agtttgcct cagcacatt 
gaggctgtt tttatacac aatataatc aagttgttt 
gctcatgta atgcgaggg tatgaggag gaggggcaa 
tatggggag attttgggg ctgtgtgat gagatttgt 
gtaaagttg ggggagagt ttgaagtca ggggagttg 
gaggtgcag gagaggtca agtacgtcg cttattatc 
gtgcagatt gtgaaggag gagattgag aaggttatg 
ggggggacg ggagaaggg gagaattca gtgtcgggg 
gatttactc gctctgttt acgggggag ttgaatccg 
gtggccccc aaagcccag aagaaggtc ccaatacca 
gaagggtta gatttggac gcttggatc aacgaaccg 
cccgaagat tcggactcg tcaagcgat cacgaggtc 
ttcgacaac cttttcatc aaaacggaa aaatcagac 
tatggcaat tcggttcgg taccaaccg gaaccgacc 
gaagaagaa atccgcaaa agtcgcgaa gcccgaaaa 
tacgaacaa caaaacaat ccgcactat ctcaaaaca 
tcagacgtt gataaaaat gcaaacgat agcaacatt 
gataacatc ccagttgct gaaattgat ttgaacatt 
ccgttgaaa gtcgtgggc caaaaacgt tccgacaaa 
tatttgaac atgaacgcg agccggaag aagacgaaa 
aagaagaac aaaaagaag aaacacaag aacgaaagt 
tcctcagac gaggaaaat gcgaatgaa ggtccagta 
gttgaagtt aaaagggac ttggagttg cccgaaggg 
gcgactttg tcggactcc gacagcagt cacgggcac 
gttgatgac ccccatagg gccctcaat atcgatcta 
gaactgccc ccagtttac gaaactcca gtcaaggcg 
gtcgaggcg aaagaggag gaaaagaaa gtgaaaaaa 
tcgaaaaag aacaagggc gagaaggag gagaaggag 
aagaagaag aagcaccat gacaagggc gcgaagaaa 
gcgaagaag gagaagaag gagaaagag ggcaagtcg 
aagaagaag aagccgacg tctggctac gaggaagcg 
ctaggtatt tcaacaccc agcaaggaa ttccaatga 
ttttctctt tatgtgcgc ctacaaatc attttataa 
tcattcaaa ttaatcatg ttattgtat tattgttta 
ttaaagaag cgataaaga t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY