Status
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Gene information
General information
TC015132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2784961..2794591
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015132
    iBeetle screen iB_05758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015132 RA
      MSHRDFTEV EPEGTSELP AANRALFLE KVRQSNTAC 
      QNGDFSTAV ALYTEALQL DPTNHILYS NRSAAKLKQ 
      GLFAQALQD AITARDLCP TWPKAYYRQ GVALQCLGR 
      HGDALAAFS QGLAQDPTS QQLLSGLVE ASIKSPLRH 
      NLEPTFQQL EVMKLDQSP FVLISVVGQ ELLAAGLYH 
      SAVTVLESA LRIGSCSLK LRGSVFSAL SSAHWALNQ 
      LDKAIGYMQ QDLAIAKSL GDTAGECRA HGNLGSAYF 
      SQGSYKEAL TAHRYQLVL AMKCKDTQA AAAALTSLG 
      HVYTAIGDY PNALASHKQ CVQLVKQMG DRLQEAREI 
      GNVGAVYLA MGDFDSAVD CHTQHLRIA RRLGNQVEE 
      ARAYSNLGS SHHYKRNFA QAIVYHEKV LRLAQAIGD 
      KSVEARAYA GLGHAARCA GDYVQAKQW HERQLDVAL 
      ATRDKVGEG RACSNLGIV YQLLGENDA ALKLHQAHL 
      AIARSLQDR AGMGRAYGN IGNAYSAAG FYESAIKYH 
      KQELTISKE VHDRSSEAS THGNLAVAY QALGAHDMA 
      LLHYRAHLG IARELKDTA GEACALLNL ANCLSSRSE 
      FAEAIPYYE QYLMLSQEL ADVEGEAKA CHFLGYAHY 
      CIGNFREAV RYYDQDLAL AKDLQDKMN MGRAYCNLG 
      LAHLALGQL ETSLECQKY FLAIAHMTN NLPGKFRAL 
      GNIGDVLIK MGETEEAVK MYHRQLALA RSNRDRGME 
      AAACGALGI AHRLLKRLD KALGYHTQE LTLRQEMSE 
      LSGECRAHG HLGAVHMAL GNYTHAVKC YQEQLERAQ 
      ELQDCAVEA QAYGNLGIA RLNMAHYED AIGYFEQQL 
      ATLERLSSP TAQLDKGRA FGNLGDCYD ALGDPEEAA 
      KCHEQHLAI ALKLKSVRD QERAYRGLG QAQKALTNL 
      QQALVCLEK RLVVAHELA NPEAKAAAY GELGQLHAN 
      LGNLEQAIS CLDHQKAIA RELNDKIME ADAISNLGC 
      VYQQMGEYT TALRYHQTD LDIAEQLQL PTLQSRACG 
      NLGAVHEAL GNLEQAIRY QEQHLSIAA STNDQHAKT 
      VAYSSLGRV HQLLGNRSQ AVAYLSQGL SIAEALGRR 
      DEEARIRNR LGLTLWSNG DLEGAQKQL EAATNLLEN 
      IRREARNTP DYKLSLFEL QSSSYQVLQ RVLVGLNRH 
      EEALVVAER GRNRAFVDL LLERQGLND GRNKKRFDD 
      VTASTLDQI KEIVNRQRA SVLYYSIAA GFLYSWLIV 
      PTKGIVKFH SKSLNESDE SDSTDPTSN IGILEKLVT 
      SVRDSLGME LSPCSTINE DQYSDDTLS DRTGFLRMV 
      NRQHLLNSS NYSLSSLFS LGSVGGSVA SLQGSTRSS 
      TSVQATSRQ AWKGPSCLH ALYTLLIQP FEDFLPKAN 
      SNRRELILV LEGDLYLVP FPVLRSSSE ASEYLCERF 
      SLLAVPNIS SLRIGRSRK QDGDQTVRS LIVGNPKLP 
      NAVTEHWGW KDIPQAEQE ATMVAELLQ AQVLLGAGA 
      TKEQILNQI QDAECVHFS THVSWKLSS LVLSPAEVL 
      DQSQKRLYL GDPAEEDEN EVSTSAELP PMSEFLLSA 
      ADILSLRLS ARLVVVSSS HTRDQQGWA TSDGLVALV 
      RALLAAGAQ AVLVSLWPV PETASKILL RAFYSAMLQ 
      GTRAAKALA EAMQTVQHT KHFAHPANW AGFLLVGLN 
      VRLSNKVAM MGQALCELL QVPDKCRDA LRVTLHLVE 
      KSLQRIHRG QKNAMYTTQ KSIENKVGS VTGWRDLLI 
      SVGFRFEPA SNGIPSSVF FPQSDPDER LTQCSASLQ 
      ALLALSNTS IHALSKLTI NPDVSDDII GVIQSALAQ 
      FTENESVEL GLNVRLWRV PGCHELLAS LGFDLTDVG 
      QDKVTLRLG KQANRRNIQ FTLQALLAL FDTQEAPKS 
      LTIDSSSSL ESLASVDND FSHSDNELC QAPKSPQLA 
      KPRLNFSRT VLPTKRLGG AFTSYVRRR GEPDGRTAN 
      PSDTKTLLS DKTRKNVGR PETEAAFTP SPPVVLQSE 
      NQNIALSLA HQTRIKNLY SQNDNKNDL RPDSSSSAS 
      SVTDWDNGH ATVLRRQTQ IPPLPPSRT KLDLTLYNN 
      LPSRLFENS SDSELEKME AKLFNTRNK MAYRKSRMS 
      TLDRLSVRT ELTPQNLLQ ASRKPITLP SEETLPTNE 
      RLLYFSPSD VDTSPQKLP TDSDESKDK PDLHDTILR 
      HINRELTPT ISDVYHERN IGLGLAPPL SKLLLNQPT 
      NATPQTDAN ALEKITLGI LEEGGEPWM AEGGLQQIQ 
      SSDLTTADI LEREVKNKK GLREGEVKR LSPFSEMSR 
      RDEGDGRSI ADSNCSSYK NRFGDYRTG EKRPVAAQR 
      RSKQT
    CDS sequence
    • >TC015132 RA
      atgtcgcac agggatttt acggaggtt gaacctgaa 
      ggcacttcc gaacttccg gcggccaat cgcgctctc 
      ttccttgaa aaggtcaga caatcaaac accgcttgt 
      caaaatggc gacttttcc acagcagtc gctttatac 
      accgaagcc cttcaattg gatccaacc aatcacatt 
      ctttattcg aatcgttcc gccgccaaa ctcaaacag 
      ggccttttt gcccaggcc cttcaggac gctattaca 
      gcacgtgac ctatgcccc acctggcct aaagcctac 
      tatcgtcaa ggcgtcgct ttacaatgt ctaggccga 
      catggcgac gccttggct gcattcagt caaggctta 
      gcccaggat ccaacctca caacaactt ctttcggga 
      ttggttgaa gcgtcgatc aaatcaccg ctgagacat 
      aatctcgaa ccgactttc cagcagctg gaagtcatg 
      aaattggat cagagtccg tttgtttta atttcggtg 
      gtggggcag gagttgttg gcggcggga ttgtatcat 
      tcagcggtg actgtgcta gaatcagca ctcagaatc 
      ggttcctgt tctttaaaa ttgcgagga tctgtgttc 
      agtgctttg agttcagct cattgggca ctaaatcag 
      ctcgataaa gcaataggt tacatgcaa caagactta 
      gccattgct aaaagccta ggtgatacg gcgggggaa 
      tgccgggca catggaaac ctaggctcg gcctatttc 
      agccaaggt tcgtacaag gaagccttg actgcgcac 
      cgataccaa ctcgtgcta gccatgaaa tgcaaggac 
      acccaggct gctgccgca gccctaact tccctaggc 
      cacgtctat acagcaatc ggtgactac ccgaacgcc 
      ttagcttcg cacaaacag tgcgtccaa ctcgttaaa 
      caaatgggc gataggttg caagaagcc cgcgaaatt 
      ggtaacgta ggcgccgta tacctagcc atgggtgac 
      tttgattcg gccgttgat tgtcacacc caacattta 
      cgaatcgcc cgtcgccta ggcaaccag gttgaagaa 
      gcacgtgct tacagcaac ctaggttct agtcaccac 
      tacaaacgc aactttgct caggcgatt gtttatcat 
      gagaaagtc ctaagattg gcacaagcc attggtgat 
      aaatcggtc gaagccagg gcttacgcc ggcctagga 
      catgcggct aggtgtgcc ggtgattac gtccaagcc 
      aaacaatgg catgaaaga caactagat gtcgcgtta 
      gcgactcgt gataaggtt ggcgaggga cgtgcatgc 
      tcaaacctg ggaatagtc tatcagttg ttgggggaa 
      aatgatgca gccttgaag ttacaccaa gcccatttg 
      gcgatcgct cgaagttta caggataga gcaggaatg 
      gggagagct tacgggaac atcgggaat gcctattcg 
      gctgcgggg ttttacgaa tccgccata aagtaccac 
      aaacaggag ctgacaatc agcaaggaa gtgcacgat 
      aggagttcc gaggctagt actcatgga aacctagcc 
      gtagcttat caagcctta ggggctcat gatatggca 
      cttttgcat tatcgggcc catctaggt attgcaagg 
      gagctaaaa gacacagct ggtgaagcc tgtgcgtta 
      ctaaacctg gccaattgt ctcagttca aggtcggaa 
      ttcgccgaa gcgataccg tactacgaa cagtatctt 
      atgctatcg caagaactc gccgatgtg gaaggtgaa 
      gctaaagcc tgtcacttt ttaggctac gcccactac 
      tgtatcggc aattttcgc gaagctgtt cgttactac 
      gaccaggat ttagcctta gccaaagac ttgcaagac 
      aaaatgaac atgggacgt gcttactgc aacctaggc 
      ttggcccat ttggctctc ggccaactt gaaacatct 
      ctcgagtgc caaaaatat tttttagcc atcgcacac 
      atgaccaat aacctaccc ggcaaattt cgggcattg 
      ggcaacata ggcgacgtt ctaatcaaa atgggcgag 
      acagaagaa gccgtgaaa atgtaccat cgccaacta 
      gccttagcc cgaagtaac cgtgatcgt ggcatggaa 
      gctgccgct tgtggtgct ttaggtata gcccaccgt 
      cttttaaaa cgcctagac aaagctcta ggctaccac 
      acccaagaa ctaaccctt cgacaagaa atgtccgaa 
      ttatcgggt gaatgccgg gctcatggc cacctcgga 
      gccgtccat atggcccta ggcaattac acccacgct 
      gttaaatgc taccaagag caactagaa cgggcccaa 
      gagctacaa gactgcgcc gttgaagcg caagcctac 
      ggcaaccta ggcatcgcc cgcctaaac atggcccac 
      tacgaagac gcgattggt tatttcgaa caacaacta 
      gcgactctc gaacgtctc agttcaccc acagcccag 
      ttggacaaa ggccgggct tttggcaac ctgggcgat 
      tgttacgac gctttaggc gacccagaa gaagctgcc 
      aaatgccac gaacaacac ctagccatt gctttgaaa 
      ctaaagagt gtccgtgac caggaacgc gcctaccgt 
      ggactaggc caagcccaa aaagcccta actaacctc 
      caacaagcc ctagtttgc cttgaaaaa cgcctagtc 
      gtcgcacac gaattggcc aatcctgaa gccaaagct 
      gcagcttat ggcgaattg ggccaactt cacgccaac 
      ttgggtaat ttagaacag gcaatttcg tgtttagac 
      caccagaag gcaatcgcg cgcgaactc aacgataag 
      ataatggaa gccgatgcg atttccaac ctgggctgc 
      gtttaccaa caaatgggc gaatacacc actgcgtta 
      cgctaccac caaaccgac ctagacata gccgaacag 
      ttacaatta ccgacgtta caaagccgc gcttgtgga 
      aacctaggc gctgtacac gaagcctta ggcaacctg 
      gagcaagca atacgctac caagaacaa cacttgtca 
      attgcggca agtaccaac gaccaacac gcgaaaacc 
      gtcgcctat agttcgcta ggtcgcgtc caccaactt 
      ttaggcaat cggagccag gctgtagcc tatttaagc 
      caaggtttg tcaatcgcc gaggcttta gggcgacgg 
      gatgaggaa gcaaggatt agaaatagg ctaggtttg 
      acgttatgg tcgaacggt gaccttgaa ggcgcccaa 
      aaacaactc gaagcggcg acaaatttg ttggaaaat 
      attagacgc gaagccagg aatacaccc gactataaa 
      ctatcactg tttgagtta caatcgtca agttatcaa 
      gtattgcaa cgtgttttg gttggttta aaccgacac 
      gaggaggct cttgttgtg gccgaaagg gggcgtaat 
      cgggctttt gtcgacttg ctgttagaa cgtcaaggt 
      ttgaatgac ggacgtaac aaaaaacgc ttcgatgat 
      gttactgcg agtaccttg gaccagatc aaggaaatt 
      gtgaaccgg caacgagct tcggtactt tattacagc 
      atagccgct ggttttctc tactcatgg ctcattgta 
      ccgaccaaa ggcatagtc aaattccac tcaaaatcg 
      ctgaatgaa agtgacgaa agtgacagt accgacccg 
      acctcaaac atcgggatt ctcgaaaag ttggtgact 
      tcggtcaga gatagtctc gggatggag ttatctccc 
      tgtagtacc ataaacgaa gatcagtac tcggacgac 
      actcttagc gaccgcacc ggattcctc cgaatggtc 
      aaccgccaa cacctcctc aattccagc aactactcc 
      ctcagctcc ctcttctcc cttgggagt gttggtggt 
      tcggtggcg tcactccaa ggctcgacc cgatcaagc 
      acaagcgtc caggcgaca tcacgccaa gcctggaaa 
      ggcccctcc tgccttcac gccctttac acgctcctg 
      atccaaccc tttgaggac tttctcccc aaagccaac 
      tcaaaccga cgagaacta atcctagtc cttgaaggt 
      gacctctat ttagtccct ttccccgtc ctacgctcc 
      tcatcggaa gcttccgaa tacctctgc gagagattc 
      tcgctactt gccgtcccc aatatatca tcattacgc 
      attgggcga agtcgcaaa caagacggt gaccaaact 
      gttcggagc cttatcgtc ggcaacccc aaattaccg 
      aacgctgtt acggaacac tggggctgg aaagacatc 
      ccgcaagcc gaacaagag gccacaatg gtggccgag 
      ttattacaa gcacaagtg ttactaggt gctggcgca 
      acgaaagaa caaattctc aatcagata caggacgct 
      gaatgtgtc catttctcg actcatgtc tcttggaag 
      ttatcctct ttggtttta agccccgct gaagtttta 
      gatcagagc cagaagagg ctctatctg ggggacccg 
      gccgaagag gacgaaaac gaagtctcg acttcggca 
      gagttgccc ccaatgtcc gaatttcta ctaagtgca 
      gccgatatt ctgtcatta cggctttcg gcacgtctt 
      gtcgtggtg agttcgtcg cacacgcgt gaccaacaa 
      ggatgggcc acttctgat ggtctggtg gccttggtg 
      cgggcgcta ctagcagcc ggtgcccaa gccgtttta 
      gtgtcactg tggcctgtg ccggaaacg gcctctaaa 
      atcctactc cgggctttt tactctgcc atgttacaa 
      ggaacacga gctgctaag gcactcgct gaagccatg 
      caaactgtc caacacacc aaacatttt gcccatcca 
      gcaaactgg gccggtttc ctccttgta ggtctaaac 
      gtccgacta tcaaacaaa gtggctatg atgggccaa 
      gccctttgt gaactcctc caagtcccc gataaatgc 
      cgggatgcc cttagagtg accctccac ttggtggaa 
      aagtccctt cagaggata caccgcggt cagaaaaac 
      gccatgtac accacgcaa aaatcaatc gagaataaa 
      gtcggttcg gtgaccgga tggcgcgac cttttgatt 
      tccgtgggc tttagattc gagcctgcg tcaaacggg 
      ataccaagc agtgtgttt ttcccccaa tcagatccc 
      gatgaacgt ctaacccaa tgttcggca agtctgcaa 
      gcccttcta gccctttca aacacttcg attcacgca 
      ctttcgaaa ctaacaatc aatcccgac gtttcggac 
      gacatcatc ggagtcatc caatcggct ttagcccaa 
      ttcaccgaa aacgaaagt gtggagcta gggctaaac 
      gtgcgcttg tggcgagtg ccaggctgt catgaactt 
      ttagcctcg ttaggcttc gacttgacc gatgtagga 
      caagataaa gtgacctta agattaggc aaacaggcc 
      aatcggcgt aacatccaa ttcacgtta caagccctt 
      ttggccctt ttcgataca caagaagcc ccaaaaagc 
      ctaacaatt gactcgtca agtagtcta gaatctttg 
      gcatcggtc gataatgat ttttcccat tcggataac 
      gaactgtgt caagcaccg aaatcccca caactggcc 
      aaaccacgc ctgaatttt tcccgcaca gtgctacca 
      accaaacga ctcggtggt gctttcacc agttatgtg 
      agacgtcgg ggcgaaccg gacggacgc acagccaac 
      cctagcgac actaaaacg ctactaagc gacaagacc 
      cgaaaaaat gttggcagg cctgaaacc gaagccgcc 
      tttacgcca agcccacca gtcgtgtta caatcggaa 
      aaccaaaac attgcacta tcactcgca caccagaca 
      cgaatcaaa aacctctac tcacaaaat gacaataaa 
      aacgaccta agacccgat agttcaagc tcagcgagt 
      tcggtcacc gactgggat aacggccat gcgactgtt 
      ttaagacgc caaacacaa atcccacct ctgccaccc 
      tcacgaacg aaactagac ctaacactc tataacaac 
      ctaccgtct cgtttgttc gaaaacagt tccgacagc 
      gaactcgaa aaaatggaa gcgaaacta ttcaacaca 
      cgcaataaa atggcatat cgcaaatcg cgaatgtca 
      acccttgac cgtctaagc gtccggact gaactcacg 
      cctcaaaac ctcctccaa gcctcacgc aaacccatc 
      acactaccc agcgaagaa accctcccg actaacgaa 
      cgcctcctt tatttctcc ccatcggac gttgacacc 
      tcccctcaa aaactcccc acagattcg gacgaatcc 
      aaagacaag cccgacctc cacgacacc atccttcgc 
      cacatcaat cgcgaactc acccccacc atctccgat 
      gtctaccac gaacgcaat atcgggctt ggtctagct 
      ccgccctta tctaaactc ctccttaat caaccgaca 
      aacgccaca ccgcaaacg gacgccaac gcacttgaa 
      aaaatcaca ttggggata ctcgaggag gggggagag 
      ccgtggatg gccgaaggc ggtttacaa caaatccaa 
      agctccgat ttgacaact gccgatatt ttggagagg 
      gaagtgaag aataagaag gggttgaga gaaggggaa 
      gtgaagaga ttgtcgccc ttttcggaa atgtcacga 
      agggacgag ggcgatggg cggagcata gccgattcg 
      aattgttca agttataaa aaccgtttt ggtgattat 
      aggacggga gagaagcga ccagtcgca gcacaaagg 
      aggagcaag cagacgtag
    mRNA sequence
    • >TC015132 RA
      cctgaccag gaacatccg gactgtgac gtaaatcaa 
      aaacgtgta atacatttg tgccaaaag caaccaact 
      taacaacaa aaagctttc aaaataatt tattgacta 
      ctttatcac agaatctct aataacaat tcaaaacac 
      attaatttt ctaatgatg gtgacacgt tagtgccca 
      aaacgttgg ctttatagc gaagttttt ctacttcag 
      tggcagatt ccaaagttt catttgaaa gaagtctgt 
      cgtcccttg attctttaa tagaggcta aaaataatt 
      atcacaata tctcataat tgaaataaa aaaatgaat 
      tagttttta ccattgtat ggtgtatat ttatcggaa 
      aaataattt tccaaatta taacctatt acaccgaca 
      actgccgca aaaggaagg gtgatataa ggggcgtag 
      acccgtagc gttcttatt aaaatgggc cacattgta 
      tacacttca aaaaaacag gatttttca cggttttca 
      cgcacttaa cctcacttt cctagggtc aaggtcaca 
      aatatcaag tgagataac cacaacttg gcttgtatt 
      acgggttgc ctaacgatt ttttttcta attattgct 
      attttaaaa caaaaagtc gtgtcattt gattcaaaa 
      tccagaaaa aaaaacatt ttttttctt tttggcact 
      ttcaatgcc aaggtcatc cgtataaat tgttaaaaa 
      ccaacaatt ccaccaaca ccttggccc cccttgacc 
      cctttgccc atttctttg gcgttcctc accaacaga 
      cttgtggtg gaatggccg cccatccac tacctgcat 
      tccaatatg gcgtcccca agaatgcgg gtaaataac 
      tgttagacg tgcacttgt tttcccgaa gatttatcg 
      tgattttaa cgaaatcga agtgtgcgc cgccgatcg 
      tcgattgtt gaaaagaaa aatggccgc catcgtcgt 
      tcgatttgt gtggtgatt taaaaatta aaaaaagag 
      tgacgtttt ttcgaatga cgatgtatt ggaaaatat 
      cgattttcc atcatgtcg cacagggat tttacggag 
      gttgaacct gaaggcact tccgaactt ccggcggcc 
      aatcgcgct ctcttcctt gaaaaggtc agacaatca 
      aacaccgct tgtcaaaat ggcgacttt tccacagca 
      gtcgcttta tacaccgaa gcccttcaa ttggatcca 
      accaatcac attctttat tcgaatcgt tccgccgcc 
      aaactcaaa cagggcctt tttgcccag gcccttcag 
      gacgctatt acagcacgt gacctatgc cccacctgg 
      cctaaagcc tactatcgt caaggcgtc gctttacaa 
      tgtctaggc cgacatggc gacgccttg gctgcattc 
      agtcaaggc ttagcccag gatccaacc tcacaacaa 
      cttctttcg ggattggtt gaagcgtcg atcaaatca 
      ccgctgaga cataatctc gaaccgact ttccagcag 
      ctggaagtc atgaaattg gatcagagt ccgtttgtt 
      ttaatttcg gtggtgggg caggagttg ttggcggcg 
      ggattgtat cattcagcg gtgactgtg ctagaatca 
      gcactcaga atcggttcc tgttcttta aaattgcga 
      ggatctgtg ttcagtgct ttgagttca gctcattgg 
      gcactaaat cagctcgat aaagcaata ggttacatg 
      caacaagac ttagccatt gctaaaagc ctaggtgat 
      acggcgggg gaatgccgg gcacatgga aacctaggc 
      tcggcctat ttcagccaa ggttcgtac aaggaagcc 
      ttgactgcg caccgatac caactcgtg ctagccatg 
      aaatgcaag gacacccag gctgctgcc gcagcccta 
      acttcccta ggccacgtc tatacagca atcggtgac 
      tacccgaac gccttagct tcgcacaaa cagtgcgtc 
      caactcgtt aaacaaatg ggcgatagg ttgcaagaa 
      gcccgcgaa attggtaac gtaggcgcc gtataccta 
      gccatgggt gactttgat tcggccgtt gattgtcac 
      acccaacat ttacgaatc gcccgtcgc ctaggcaac 
      caggttgaa gaagcacgt gcttacagc aacctaggt 
      tctagtcac cactacaaa cgcaacttt gctcaggcg 
      attgtttat catgagaaa gtcctaaga ttggcacaa 
      gccattggt gataaatcg gtcgaagcc agggcttac 
      gccggccta ggacatgcg gctaggtgt gccggtgat 
      tacgtccaa gccaaacaa tggcatgaa agacaacta 
      gatgtcgcg ttagcgact cgtgataag gttggcgag 
      ggacgtgca tgctcaaac ctgggaata gtctatcag 
      ttgttgggg gaaaatgat gcagccttg aagttacac 
      caagcccat ttggcgatc gctcgaagt ttacaggat 
      agagcagga atggggaga gcttacggg aacatcggg 
      aatgcctat tcggctgcg gggttttac gaatccgcc 
      ataaagtac cacaaacag gagctgaca atcagcaag 
      gaagtgcac gataggagt tccgaggct agtactcat 
      ggaaaccta gccgtagct tatcaagcc ttaggggct 
      catgatatg gcacttttg cattatcgg gcccatcta 
      ggtattgca agggagcta aaagacaca gctggtgaa 
      gcctgtgcg ttactaaac ctggccaat tgtctcagt 
      tcaaggtcg gaattcgcc gaagcgata ccgtactac 
      gaacagtat cttatgcta tcgcaagaa ctcgccgat 
      gtggaaggt gaagctaaa gcctgtcac tttttaggc 
      tacgcccac tactgtatc ggcaatttt cgcgaagct 
      gttcgttac tacgaccag gatttagcc ttagccaaa 
      gacttgcaa gacaaaatg aacatggga cgtgcttac 
      tgcaaccta ggcttggcc catttggct ctcggccaa 
      cttgaaaca tctctcgag tgccaaaaa tatttttta 
      gccatcgca cacatgacc aataaccta cccggcaaa 
      tttcgggca ttgggcaac ataggcgac gttctaatc 
      aaaatgggc gagacagaa gaagccgtg aaaatgtac 
      catcgccaa ctagcctta gcccgaagt aaccgtgat 
      cgtggcatg gaagctgcc gcttgtggt gctttaggt 
      atagcccac cgtctttta aaacgccta gacaaagct 
      ctaggctac cacacccaa gaactaacc cttcgacaa 
      gaaatgtcc gaattatcg ggtgaatgc cgggctcat 
      ggccacctc ggagccgtc catatggcc ctaggcaat 
      tacacccac gctgttaaa tgctaccaa gagcaacta 
      gaacgggcc caagagcta caagactgc gccgttgaa 
      gcgcaagcc tacggcaac ctaggcatc gcccgccta 
      aacatggcc cactacgaa gacgcgatt ggttatttc 
      gaacaacaa ctagcgact ctcgaacgt ctcagttca 
      cccacagcc cagttggac aaaggccgg gcttttggc 
      aacctgggc gattgttac gacgcttta ggcgaccca 
      gaagaagct gccaaatgc cacgaacaa cacctagcc 
      attgctttg aaactaaag agtgtccgt gaccaggaa 
      cgcgcctac cgtggacta ggccaagcc caaaaagcc 
      ctaactaac ctccaacaa gccctagtt tgccttgaa 
      aaacgccta gtcgtcgca cacgaattg gccaatcct 
      gaagccaaa gctgcagct tatggcgaa ttgggccaa 
      cttcacgcc aacttgggt aatttagaa caggcaatt 
      tcgtgttta gaccaccag aaggcaatc gcgcgcgaa 
      ctcaacgat aagataatg gaagccgat gcgatttcc 
      aacctgggc tgcgtttac caacaaatg ggcgaatac 
      accactgcg ttacgctac caccaaacc gacctagac 
      atagccgaa cagttacaa ttaccgacg ttacaaagc 
      cgcgcttgt ggaaaccta ggcgctgta cacgaagcc 
      ttaggcaac ctggagcaa gcaatacgc taccaagaa 
      caacacttg tcaattgcg gcaagtacc aacgaccaa 
      cacgcgaaa accgtcgcc tatagttcg ctaggtcgc 
      gtccaccaa cttttaggc aatcggagc caggctgta 
      gcctattta agccaaggt ttgtcaatc gccgaggct 
      ttagggcga cgggatgag gaagcaagg attagaaat 
      aggctaggt ttgacgtta tggtcgaac ggtgacctt 
      gaaggcgcc caaaaacaa ctcgaagcg gcgacaaat 
      ttgttggaa aatattaga cgcgaagcc aggaataca 
      cccgactat aaactatca ctgtttgag ttacaatcg 
      tcaagttat caagtattg caacgtgtt ttggttggt 
      ttaaaccga cacgaggag gctcttgtt gtggccgaa 
      agggggcgt aatcgggct tttgtcgac ttgctgtta 
      gaacgtcaa ggtttgaat gacggacgt aacaaaaaa 
      cgcttcgat gatgttact gcgagtacc ttggaccag 
      atcaaggaa attgtgaac cggcaacga gcttcggta 
      ctttattac agcatagcc gctggtttt ctctactca 
      tggctcatt gtaccgacc aaaggcata gtcaaattc 
      cactcaaaa tcgctgaat gaaagtgac gaaagtgac 
      agtaccgac ccgacctca aacatcggg attctcgaa 
      aagttggtg acttcggtc agagatagt ctcgggatg 
      gagttatct ccctgtagt accataaac gaagatcag 
      tactcggac gacactctt agcgaccgc accggattc 
      ctccgaatg gtcaaccgc caacacctc ctcaattcc 
      agcaactac tccctcagc tccctcttc tcccttggg 
      agtgttggt ggttcggtg gcgtcactc caaggctcg 
      acccgatca agcacaagc gtccaggcg acatcacgc 
      caagcctgg aaaggcccc tcctgcctt cacgccctt 
      tacacgctc ctgatccaa ccctttgag gactttctc 
      cccaaagcc aactcaaac cgacgagaa ctaatccta 
      gtccttgaa ggtgacctc tatttagtc cctttcccc 
      gtcctacgc tcctcatcg gaagcttcc gaatacctc 
      tgcgagaga ttctcgcta cttgccgtc cccaatata 
      tcatcatta cgcattggg cgaagtcgc aaacaagac 
      ggtgaccaa actgttcgg agccttatc gtcggcaac 
      cccaaatta ccgaacgct gttacggaa cactggggc 
      tggaaagac atcccgcaa gccgaacaa gaggccaca 
      atggtggcc gagttatta caagcacaa gtgttacta 
      ggtgctggc gcaacgaaa gaacaaatt ctcaatcag 
      atacaggac gctgaatgt gtccatttc tcgactcat 
      gtctcttgg aagttatcc tctttggtt ttaagcccc 
      gctgaagtt ttagatcag agccagaag aggctctat 
      ctgggggac ccggccgaa gaggacgaa aacgaagtc 
      tcgacttcg gcagagttg cccccaatg tccgaattt 
      ctactaagt gcagccgat attctgtca ttacggctt 
      tcggcacgt cttgtcgtg gtgagttcg tcgcacacg 
      cgtgaccaa caaggatgg gccacttct gatggtctg 
      gtggccttg gtgcgggcg ctactagca gccggtgcc 
      caagccgtt ttagtgtca ctgtggcct gtgccggaa 
      acggcctct aaaatccta ctccgggct ttttactct 
      gccatgtta caaggaaca cgagctgct aaggcactc 
      gctgaagcc atgcaaact gtccaacac accaaacat 
      tttgcccat ccagcaaac tgggccggt ttcctcctt 
      gtaggtcta aacgtccga ctatcaaac aaagtggct 
      atgatgggc caagccctt tgtgaactc ctccaagtc 
      cccgataaa tgccgggat gcccttaga gtgaccctc 
      cacttggtg gaaaagtcc cttcagagg atacaccgc 
      ggtcagaaa aacgccatg tacaccacg caaaaatca 
      atcgagaat aaagtcggt tcggtgacc ggatggcgc 
      gaccttttg atttccgtg ggctttaga ttcgagcct 
      gcgtcaaac gggatacca agcagtgtg tttttcccc 
      caatcagat cccgatgaa cgtctaacc caatgttcg 
      gcaagtctg caagccctt ctagccctt tcaaacact 
      tcgattcac gcactttcg aaactaaca atcaatccc 
      gacgtttcg gacgacatc atcggagtc atccaatcg 
      gctttagcc caattcacc gaaaacgaa agtgtggag 
      ctagggcta aacgtgcgc ttgtggcga gtgccaggc 
      tgtcatgaa cttttagcc tcgttaggc ttcgacttg 
      accgatgta ggacaagat aaagtgacc ttaagatta 
      ggcaaacag gccaatcgg cgtaacatc caattcacg 
      ttacaagcc cttttggcc cttttcgat acacaagaa 
      gccccaaaa agcctaaca attgactcg tcaagtagt 
      ctagaatct ttggcatcg gtcgataat gatttttcc 
      cattcggat aacgaactg tgtcaagca ccgaaatcc 
      ccacaactg gccaaacca cgcctgaat ttttcccgc 
      acagtgcta ccaaccaaa cgactcggt ggtgctttc 
      accagttat gtgagacgt cggggcgaa ccggacgga 
      cgcacagcc aaccctagc gacactaaa acgctacta 
      agcgacaag acccgaaaa aatgttggc aggcctgaa 
      accgaagcc gcctttacg ccaagccca ccagtcgtg 
      ttacaatcg gaaaaccaa aacattgca ctatcactc 
      gcacaccag acacgaatc aaaaacctc tactcacaa 
      aatgacaat aaaaacgac ctaagaccc gatagttca 
      agctcagcg agttcggtc accgactgg gataacggc 
      catgcgact gttttaaga cgccaaaca caaatccca 
      cctctgcca ccctcacga acgaaacta gacctaaca 
      ctctataac aacctaccg tctcgtttg ttcgaaaac 
      agttccgac agcgaactc gaaaaaatg gaagcgaaa 
      ctattcaac acacgcaat aaaatggca tatcgcaaa 
      tcgcgaatg tcaaccctt gaccgtcta agcgtccgg 
      actgaactc acgcctcaa aacctcctc caagcctca 
      cgcaaaccc atcacacta cccagcgaa gaaaccctc 
      ccgactaac gaacgcctc ctttatttc tccccatcg 
      gacgttgac acctcccct caaaaactc cccacagat 
      tcggacgaa tccaaagac aagcccgac ctccacgac 
      accatcctt cgccacatc aatcgcgaa ctcaccccc 
      accatctcc gatgtctac cacgaacgc aatatcggg 
      cttggtcta gctccgccc ttatctaaa ctcctcctt 
      aatcaaccg acaaacgcc acaccgcaa acggacgcc 
      aacgcactt gaaaaaatc acattgggg atactcgag 
      gagggggga gagccgtgg atggccgaa ggcggttta 
      caacaaatc caaagctcc gatttgaca actgccgat 
      attttggag agggaagtg aagaataag aaggggttg 
      agagaaggg gaagtgaag agattgtcg cccttttcg 
      gaaatgtca cgaagggac gagggcgat gggcggagc 
      atagccgat tcgaattgt tcaagttat aaaaaccgt 
      tttggtgat tataggacg ggagagaag cgaccagtc 
      gcagcacaa aggaggagc aagcagacg tagggggcg 
      gggtttatt tattttgtg atttttttt taatatagc 
      taagtgaga aaagtggaa taaacgaaa gactttgta 
      tttaattaa ttggtttta ataaaaaat aaacataat 
      t
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