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Gene information
General information
TC015127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2745007..2747159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015127
    iBeetle screen iB_05756

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015127 RA
      MMKFKVTGL VADLMPNIR LIQASGHFM LNYHADNSG 
      ALHTLRLGY CCMHLVFVL VQYGCNFVN LVLERGDVN 
      DLAANTITV LFFTHCVTK FVYFAVRSK LFYRTLGIW 
      NQPNSHPLF VESNNRYHG IALKKMRRL LYIIIIWTS 
      FSAIAWTGI TFVGDSVHN IKDPENENL TITEPIPRL 
      LVKAWYPWD AMSGMPYYI TLVFQIYYV FFSLAHANL 
      LDSLFCSWL IFACEQLQH LKEIMKPLM ELSATLDTY 
      VPKSADLFR APSATSQDQ LIENDYNEK NEDLKGVYS 
      TRQELGGHF RGGALQNFG SGGVGPNGL TKKQELMVR 
      SAIKYWVER HKHVVRLVT AIGDAYGVA LLLHMLTST 
      IMLTLLAYQ ATKITGVDK YAATVLGYL LFALAQVFH 
      FCIFGNRLI EESSSVMEA AYSCHWYDG SEEAKTFVQ 
      IVCQQCQKA MSISGAKFF TISLDLFAS VLGAVVTYF 
      MVLVQLK
    CDS sequence
    • >TC015127 RA
      atgatgaaa ttcaaggtc acgggcctc gtggccgac 
      cttatgccc aacattcgg ctcatccaa gcgtccggg 
      cacttcatg ctcaactac catgccgat aattccggc 
      gccttgcac accctcagg ctcggctac tgctgcatg 
      cacctggtg ttcgtccta gtccaatat ggttgtaat 
      ttcgttaat ttggtactg gaacgtggt gacgtcaac 
      gatttggct gccaacacc atcacggtg ctgtttttc 
      acgcattgt gtcacgaaa ttcgtgtat ttcgcggtc 
      aggtcgaaa cttttctac cgaacttta ggtatttgg 
      aaccaacca aacagtcat ccgttgttt gttgagtcg 
      aataatcgc taccacggc attgctttg aaaaaaatg 
      cggcgcttg ctctatatt ataatcatc tggacgtcc 
      ttcagtgct attgcttgg accggtatc acgttcgtg 
      ggtgatagc gtccacaat atcaaggac ccagaaaac 
      gaaaatttg accatcact gaacccatc ccacgcttg 
      ttggtcaag gcatggtac ccatgggac gccatgagc 
      ggcatgccc tactacata accctcgtg ttccaaatc 
      tactacgtt tttttctca ctcgcgcac gcaaacctc 
      ctcgatagc ctcttctgt tcgtggctg attttcgcc 
      tgcgagcag ttgcaacac ttgaaagag atcatgaag 
      cctttgatg gaactttcg gcaactctg gacacctac 
      gtgccgaag agtgccgac ttgttccga gcgccgagc 
      gccacctcg caggaccaa ctcatcgaa aatgattac 
      aatgagaaa aacgaggat cttaagggg gtgtatagc 
      acgcgccag gagctcggc ggccatttt aggggcggg 
      gcgttgcag aatttcggt agtgggggc gttgggccg 
      aacggtttg accaaaaaa caggagttg atggtcagg 
      tcggcgatc aaatactgg gtggagcgc cacaaacac 
      gtcgtcagg ttggtcacg gccatcggt gatgcgtat 
      ggtgtggca ctcctgctc catatgctg acgtctacg 
      atcatgttg acgcttttg gcctatcag gctacgaaa 
      atcacaggg gtggacaaa tacgcagct acggttctg 
      ggctatttg ctgtttgcg ttagcccaa gtgtttcat 
      ttttgtatt tttggcaac cgcctcatt gaggagagc 
      tcttcggtg atggaggcg gcctatagt tgccattgg 
      tacgacggg tcggaggag gctaagact tttgtacag 
      atcgtctgc caacaatgt cagaaggct atgtccatt 
      tccggtgct aaattcttc acgatttcg ttagatctg 
      ttcgcttcg gtactgggc gctgtggtg acctacttc 
      atggtgttg gtgcaactc aaatga
    mRNA sequence
    • >TC015127 RA
      actgtttca cccccatcc acccacgta ccacttatt 
      cccatcacc agtcgagtg cggaggttc catctgaca 
      acataccga tgtgggtgt gagcgaatt tcaagcaat 
      tcaagatga tgaaattca aggtcacgg gcctcgtgg 
      ccgacctta tgcccaaca ttcggctca tccaagcgt 
      ccgggcact tcatgctca actaccatg ccgataatt 
      ccggcgcct tgcacaccc tcaggctcg gctactgct 
      gcatgcacc tggtgttcg tcctagtcc aatatggtt 
      gtaatttcg ttaatttgg tactggaac gtggtgacg 
      tcaacgatt tggctgcca acaccatca cggtgctgt 
      ttttcacgc attgtgtca cgaaattcg tgtatttcg 
      cggtcaggt cgaaacttt tctaccgaa ctttaggta 
      tttggaacc aaccaaaca gtcatccgt tgtttgttg 
      agtcgaata atcgctacc acggcattg ctttgaaaa 
      aaatgcggc gcttgctct atattataa tcatctgga 
      cgtccttca gtgctattg cttggaccg gtatcacgt 
      tcgtgggtg atagcgtcc acaatatca aggacccag 
      aaaacgaaa atttgacca tcactgaac ccatcccac 
      gcttgttgg tcaaggcat ggtacccat gggacgcca 
      tgagcggca tgccctact acataaccc tcgtgttcc 
      aaatctact acgtttttt tctcactcg cgcacgcaa 
      acctcctcg atagcctct tctgttcgt ggctgattt 
      tcgcctgcg agcagttgc aacacttga aagagatca 
      tgaagcctt tgatggaac tttcggcaa ctctggaca 
      cctacgtgc cgaagagtg ccgacttgt tccgagcgc 
      cgagcgcca cctcgcagg accaactca tcgaaaatg 
      attacaatg agaaaaacg aggatctta agggggtgt 
      atagcacgc gccaggagc tcggcggcc attttaggg 
      gcggggcgt tgcagaatt tcggtagtg ggggcgttg 
      ggccgaacg gtttgacca aaaaacagg agttgatgg 
      tcaggtcgg cgatcaaat actgggtgg agcgccaca 
      aacacgtcg tcaggttgg tcacggcca tcggtgatg 
      cgtatggtg tggcactcc tgctccata tgctgacgt 
      ctacgatca tgttgacgc ttttggcct atcaggcta 
      cgaaaatca caggggtgg acaaatacg cagctacgg 
      ttctgggct atttgctgt ttgcgttag cccaagtgt 
      ttcattttt gtatttttg gcaaccgcc tcattgagg 
      agagctctt cggtgatgg aggcggcct atagttgcc 
      attggtacg acgggtcgg aggaggcta agacttttg 
      tacagatcg tctgccaac aatgtcaga aggctatgt 
      ccatttccg gtgctaaat tcttcacga tttcgttag 
      atctgttcg cttcggtac tgggcgctg tggtgacct 
      acttcatgg tgttggtgc aactcaaat gaggggttt 
      ttcgttgcg tacggttgg tagttcctt aatttctgc 
      acgtgacgt tttgaaaat tgtgataat tagacggca 
      ctgattttt caagttaca cagcattca taagtgtct 
      taattcaag atggcgagt acctgataa tggcggtga 
      caaggggaa ataataacg ctctagact tagttactt 
      ctattttac ctaataaag tttacccta acta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY