Status
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Gene information
General information
TC033382
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015122,TC015121
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2651478..2666359
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033382
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05755

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva lateral bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033382 RA
      MSSVLLEAR PFRPFKSSE EYLVAMKED LAEWLQTMY 
      PSLEINVDN FMEKLETGV VLCEHANAV RHQAAEYVE 
      KKQSKKIMT KSVTGNLAH AQKLVNIPE VKYFTTAKP 
      QTFFARDNV SNFIRWCRN SLQILECLL FESDDLILR 
      KNEKHVILC LLEVGREAA KFGMLAPML VQMERQIDR 
      EIAADQKKL NNGLHDQNS FDDDDDSDM EEETMLIYG 
      PVPQIVTND LKSLDEMVR DLVAKCTCP TQFPMIRVS 
      EGKYRIGDT KVLIFVRIL RNHVMVRVG GGWDTLSHY 
      LDKHDPCRC RSQHRTTQG ARLVNKPGA PDLHGSHVY 
      YDRAPIPTI ATPSHTSSS ASTLGPPMN SLNRSRSRS 
      PSYLNDRRS VSPNLPRKS LVPPTRSRS PTPNYTSSH 
      HATLKTKAT PTTSPKHKN HVALIQVPI ENRLKSETS 
      IQINEDQSD TTSEVSDEG YRSLGAIQD KTKKRLSQD 
      SVDDADYNG EHHIDSIDD TNDVGLRKT SYSQRIYEE 
      ATPKPLPKS REPSVDRST PKSSPKKIH GTWNGRQKQ 
      ARPTLQIET FSPPLQRHA PSRRSERRH SSASTSPTK 
      GGLKEVLIN TVTQIDDEA LMAQQVQEL LKQYTFDFK 
      DEGRFVRGG SERMSYRSP RKDSRPDNN LSRIPAPVL
    CDS sequence
    • >TC033382 RA
      atgagttca gtactattg gaagcacgg cccttcagg 
      cccttcaag tctagtgaa gagtatcta gtggccatg 
      aaggaagat ttagccgaa tggctccaa actatgtac 
      ccatcactc gaaatcaac gtcgacaac ttcatggag 
      aagttggaa acgggagtt gtactttgt gagcacgcc 
      aatgccgtc aggcatcaa gcggccgaa tatgttgaa 
      aaaaaacag tcgaagaaa atcatgaca aaatcggtg 
      actggcaat ttggcacat gcacaaaaa ctagtaaac 
      attccggaa gtcaagtac ttcacgacg gccaagccg 
      caaactttc ttcgccaga gacaacgtt tcgaatttt 
      attagatgg tgcaggaac tcgctgcag atcttagag 
      tgcttgctg tttgaatca gacgacttg attttgagg 
      aagaatgaa aaacacgtc attttgtgt cttttggaa 
      gtgggaagg gaggccgca aagttcggc atgttggcc 
      cccatgttg gtgcaaatg gagcgccaa atcgaccgc 
      gagatcgcc gccgaccag aaaaaactc aacaacggc 
      ctccacgac cagaacagc ttcgacgac gacgacgac 
      agcgatatg gaggaagaa acaatgtta atttacggt 
      cctgtgcca caaatcgtc accaacgac ttgaaaagt 
      ctcgacgaa atggtccgc gacttggtc gcaaagtgc 
      acttgtcca acccaattc cccatgatc cgtgtctcc 
      gaaggcaaa taccgaatc ggcgacacc aaagtatta 
      attttcgtg aggatactt cgaaaccac gtaatggtc 
      cgagttggc ggtggatgg gacactctg tcccattat 
      ttggacaaa cacgaccca tgtcgttgt cgctcgcaa 
      cacagaaca acccaaggc gcccgtctg gtcaataaa 
      ccaggtgcc ccagactta cacggttca cacgtttac 
      tacgaccgt gcgccaatc ccgacaatt gcaactcca 
      agccacacc tccagctcc gcctcaact ctaggccca 
      ccaatgaac tcactgaac cggtcccgg tcacgttca 
      ccgtcttat ttgaacgac agacgatca gttagtcca 
      aacctaccg cgcaaatct ttagtacct cccactaga 
      tccagaagc cccaccccc aactacaca tcgtcacac 
      cacgccacg cttaaaact aaagccacg cccacaacg 
      tcaccaaaa cacaaaaat cacgtcgcc ttgatacaa 
      gtccccatt gaaaatcgg cttaaatcc gaaacttcg 
      atccaaata aatgaagat caaagtgat acgacgtcc 
      gaagtttcg gacgaaggt taccgaagt ttgggcgca 
      attcaggac aaaaccaaa aaacgcctc agtcaagac 
      tcagtcgat gatgcagat tataacgga gagcatcat 
      attgacagt attgacgac acaaacgac gtcggtttg 
      cgtaaaacc tcctactcg cagcgaatc tacgaggag 
      gccacgccc aagcccctc cccaaatcg cgcgagcct 
      agtgttgat cgctccacc cctaaatcc tcccccaag 
      aaaatccac ggtacgtgg aacggccgt cagaaacag 
      gcacgtccc acccttcaa atcgagact ttcagtccg 
      cccctgcaa agacacgcc ccctcgagg cggagcgaa 
      cgtcgccat tcgtcggca agcacatcg ccgaccaaa 
      ggcgggctt aaagaagtt ttgataaat acagtaacg 
      caaatcgat gatgaagct ttaatggcc caacaagtc 
      caagaactg ctaaaacag tacactttt gatttcaag 
      gacgagggg cggtttgtg agaggcggc agcgaaagg 
      atgtcctac aggagtccg aggaaggac tcacggcct 
      gataataac ctatctagg atccccgcc cccgttttg 
      tag
    mRNA sequence
    • >TC033382 RA
      tgtcacatt ttacaacaa acatattat aatttaaca 
      agaagcgaa gttccattc ttgatggta gtggtggat 
      ctcgatatt gtaaatgag tagttagaa tgtacagtc 
      agttgactt agaaacgtc gtggtgaac gctaccgca 
      atgctcgct gcgcgatta tcgtgcgtt cggtgtgtg 
      tgattttgg ttttttttt ttgggcaca ctgtatata 
      tagagtaga ttgcgtcca acttccaac cagtgatgc 
      gctaagcct cccaagatg agttcagta ctattggaa 
      gcacggccc ttcaggccc ttcaagtct agtgaagag 
      tatctagtg gccatgaag gaagattta gccgaatgg 
      ctccaaact atgtaccca tcactcgaa atcaacgtc 
      gacaacttc atggagaag ttggaaacg ggagttgta 
      ctttgtgag cacgccaat gccgtcagg catcaagcg 
      gccgaatat gttgaaaaa aaacagtcg aagaaaatc 
      atgacaaaa tcggtgact ggcaatttg gcacatgca 
      caaaaacta gtaaacatt ccggaagtc aagtacttc 
      acgacggcc aagccgcaa actttcttc gccagagac 
      aacgtttcg aattttatt agatggtgc aggaactcg 
      ctgcagatc ttagagtgc ttgctgttt gaatcagac 
      gacttgatt ttgaggaag aatgaaaaa cacgtcatt 
      ttgtgtctt ttggaagtg ggaagggag gccgcaaag 
      ttcggcatg ttggccccc atgttggtg caaatggag 
      cgccaaatc gaccgcgag atcgccgcc gaccagaaa 
      aaactcaac aacggcctc cacgaccag aacagcttc 
      gacgacgac gacgacagc gatatggag gaagaaaca 
      atgttaatt tacggtcct gtgccacaa atcgtcacc 
      aacgacttg aaaagtctc gacgaaatg gtccgcgac 
      ttggtcgca aagtgcact tgtccaacc caattcccc 
      atgatccgt gtctccgaa ggcaaatac cgaatcggc 
      gacaccaaa gtattaatt ttcgtgagg atacttcga 
      aaccacgta atggtccga gttggcggt ggatgggac 
      actctgtcc cattatttg gacaaacac gacccatgt 
      cgttgtcgc tcgcaacac agaacaacc caaggcgcc 
      cgtctggtc aataaacca ggtgcccca gacttacac 
      ggttcacac gtttactac gaccgtgcg ccaatcccg 
      acaattgca actccaagc cacacctcc agctccgcc 
      tcaactcta ggcccacca atgaactca ctgaaccgg 
      tcccggtca cgttcaccg tcttatttg aacgacaga 
      cgatcagtt agtccaaac ctaccgcgc aaatcttta 
      gtacctccc actagatcc agaagcccc acccccaac 
      tacacatcg tcacaccac gccacgctt aaaactaaa 
      gccacgccc acaacgtca ccaaaacac aaaaatcac 
      gtcgccttg atacaagtc cccattgaa aatcggctt 
      aaatccgaa acttcgatc caaataaat gaagatcaa 
      agtgatacg acgtccgaa gtttcggac gaaggttac 
      cgaagtttg ggcgcaatt caggacaaa accaaaaaa 
      cgcctcagt caagactca gtcgatgat gcagattat 
      aacggagag catcatatt gacagtatt gacgacaca 
      aacgacgtc ggtttgcgt aaaacctcc tactcgcag 
      cgaatctac gaggaggcc acgcccaag cccctcccc 
      aaatcgcgc gagcctagt gttgatcgc tccacccct 
      aaatcctcc cccaagaaa atccacggt acgtggaac 
      ggccgtcag aaacaggca cgtcccacc cttcaaatc 
      gagactttc agtccgccc ctgcaaaga cacgccccc 
      tcgaggcgg agcgaacgt cgccattcg tcggcaagc 
      acatcgccg accaaaggc gggcttaaa gaagttttg 
      ataaataca gtaacgcaa atcgatgat gaagcttta 
      atggcccaa caagtccaa gaactgcta aaacagtac 
      acttttgat ttcaaggac gaggggcgg tttgtgaga 
      ggcggcagc gaaaggatg tcctacagg agtccgagg 
      aaggactca cggcctgat aataaccta tctaggatc 
      cccgccccc gttttgtag acttggggg attttctag 
      ggtattttt ttacgacgg ttagagtag agtccctgt 
      ttcgaccgt tttgttttt ggtgctaat ttgatgtga 
      tgatttttc aaattttta tcatgttgt tacgagaaa 
      aaatgctca atttttttc gatttgaac ggttagttt 
      tgagaggat accaccagt ttagattat atttaaaca 
      ccctgtata acactaata atggattta gtgcaactt 
      ttaacatgc tactggcaa cttataggt ctaattata 
      ttaatttat acttaattt tgtataaaa caaccatta 
      acatttgat attttctat tatacgttt ttaattaaa 
      aaaaaaata actaatttt atgataatt cagatttta 
      gaatctcgg ttcacacaa gtagaaacc ctatttcat 
      accattgta tgcttttct aaggcaata aatcgtaga 
      ttttaattt attcgactt tatctcctc cccgacata 
      tacactaaa taaaaaaat attttaata aaaaaacaa 
      caaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY