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Gene information
General information
TC015120 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2639668..2647679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015120
    iBeetle screen iB_05754

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015120 RA
      MDSDGHWLI GAGLVTSSI IAITANAFL LLIFCRRRG 
      LRTVPNRFV INLLITNLL SSLLLIPLL IVDQSQPAP 
      HPANATNES VEISNLVII ENSTNLEII ESLLSTVTY 
      FNSTPALAN QPILCYAAQ GGTSFVCTL SIFSILLIG 
      INQYFGVIH SLRYHFYIN KCRSTVFIL TTWLVALVL 
      SVLSSVTES DSYLWRFCR NRPQNSRTV QILNTIYAL 
      SYCLAVILV PFIAICVIY VCIYTAAHK NSERMRKST 
      STNLDCCYS NEQRAPLPK VRSAPNFMR FEEIANPKK 
      VTRTSSERA SNNFITSLK HKISNASVF KYREETRAA 
      KISVLVIFL VLFCYVPYG VTLVLNSGL VSVATSPMV 
      NFHTLLLLV VSNVISPFL FGYRNKRVK REIGKVFGL 
      VPAKAELTV QNRRRSTND EPMTSKEPF LGPNCTVPE 
      VIVTCKVEN ERKSILKRV CQSSNWTSY KKCNFITVP 
      ESCISGEAR GSFSSASTQ ISNEDC
    CDS sequence
    • >TC015120 RA
      atggattcg gacggccat tggctgatc ggtgcaggc 
      ctggtgacg tcatcaatc atcgccatc accgccaat 
      gccttccta cttctgatt ttttgtcgc agaagaggg 
      ctaaggacc gtccccaac agatttgtt atcaacttg 
      ctgatcacg aatctcctg tcaagtctt ttactgatc 
      cccctcttg atcgtcgac caatcgcag cccgcccct 
      caccccgcc aacgcgacc aacgagagt gtcgaaatt 
      tcgaatttg gtgatcatc gaaaattcg acaaatctc 
      gaaatcatt gaaagtcta ctctcgact gtcacgtat 
      ttcaactca acgccggcc ctcgccaac caaccaatc 
      ctgtgctac gccgcacag gggggcaca agtttcgtc 
      tgcaccctg tccatcttc agtattctc ctcatcgga 
      atcaaccaa tactttggc gtcatccac tcgctacgc 
      taccacttc tacatcaac aaatgccga tcgactgtc 
      ttcatcctc accacgtgg ctggtggcg ctagtgttg 
      agtgtctta agcagtgtg acggaaagt gacagttac 
      ttgtggcgc ttctgtcgc aatagacca caaaattcg 
      cgaactgtt caaatccta aacacgatt tacgcactt 
      tcctactgt ctagcagtt attttagtg ccgtttatc 
      gcaatctgt gtcatctac gtgtgcata tacacagcg 
      gcgcataaa aacagtgag cgcatgcgc aagtcaacg 
      tcaacaaac ctcgactgt tgctatagc aacgagcag 
      agggcgccg ctacctaaa gttcgctca gcgcctaac 
      ttcatgcga tttgaggaa attgccaac ccgaaaaaa 
      gtcactagg acgagcagc gaacgcgcg agtaacaat 
      ttcataaca tcgttgaaa cacaaaatt tcaaacgca 
      tcagttttt aaatatcgc gaagaaact cgagcggca 
      aaaatcagc gttctggtg atttttctc gtattgttt 
      tgctacgtc ccgtatggt gtgacactg gtcctaaat 
      tcggggcta gtaagcgtg gcaacgtcg ccaatggtc 
      aatttccat actctgctc ctattggtg gtttcgaac 
      gtgatctcg ccctttttg ttcggttat cgcaacaaa 
      agagtgaaa cgggagata ggaaaagtg tttggattg 
      gttccggct aaggccgag ctcaccgtc cagaaccgg 
      cggagatcc acgaacgat gaaccaatg accagcaag 
      gagcctttt ttagggccg aactgtacc gtacccgaa 
      gtgatagtc acgtgtaag gtggaaaac gagagaaaa 
      agtattctg aagcgggtg tgtcagagt tcgaattgg 
      acgagttac aaaaaatgt aattttatt acggtacct 
      gaaagctgt atcagcggc gaggccaga gggtcgttc 
      tccagtgcc agcacgcaa atttctaat gaagactgt 
      tag
    mRNA sequence
    • >TC015120 RA
      caccataca atggtaata tcgtagaca aaccgtttt 
      atatcgacg gctcgctcc cgacttgga atcagttgt 
      gaattagta gtgttgaaa tgcggtcgg gcatttcaa 
      tcagactac ggcaactag atgtggaaa aagtcgagt 
      tgtttactg tgaacaggg ccttaagtt agtgcatgc 
      agctgctgt tggcctgct ccagatcga aaataccgt 
      ctttttcac acagagttt cggtcacac cagtggcgt 
      acgtcgtga aagtgtgcg aaatggaga aatcgcaac 
      agtgagaag gtaatcaat ggattcgga cggccattg 
      gctgatcgg tgcaggcct ggtgacgtc atcaatcat 
      cgccatcac cgccaatgc cttcctact tctgatttt 
      ttgtcgcag aagagggct aaggaccgt ccccaacag 
      atttgttat caacttgct gatcacgaa tctcctgtc 
      aagtctttt actgatccc cctcttgat cgtcgacca 
      atcgcagcc cgcccctca ccccgccaa cgcgaccaa 
      cgagagtgt cgaaatttc gaatttggt gatcatcga 
      aaattcgac aaatctcga aatcattga aagtctact 
      ctcgactgt cacgtattt caactcaac gccggccct 
      cgccaacca accaatcct gtgctacgc cgcacaggg 
      gggcacaag tttcgtctg caccctgtc catcttcag 
      tattctcct catcggaat caaccaata ctttggcgt 
      catccactc gctacgcta ccacttcta catcaacaa 
      atgccgatc gactgtctt catcctcac cacgtggct 
      ggtggcgct agtgttgag tgtcttaag cagtgtgac 
      ggaaagtga cagttactt gtggcgctt ctgtcgcaa 
      tagaccaca aaattcgcg aactgttca aatcctaaa 
      cacgattta cgcactttc ctactgtct agcagttat 
      tttagtgcc gtttatcgc aatctgtgt catctacgt 
      gtgcatata cacagcggc gcataaaaa cagtgagcg 
      catgcgcaa gtcaacgtc aacaaacct cgactgttg 
      ctatagcaa cgagcagag ggcgccgct acctaaagt 
      tcgctcagc gcctaactt catgcgatt tgaggaaat 
      tgccaaccc gaaaaaagt cactaggac gagcagcga 
      acgcgcgag taacaattt cataacatc gttgaaaca 
      caaaatttc aaacgcatc agtttttaa atatcgcga 
      agaaactcg agcggcaaa aatcagcgt tctggtgat 
      ttttctcgt attgttttg ctacgtccc gtatggtgt 
      gacactggt cctaaattc ggggctagt aagcgtggc 
      aacgtcgcc aatggtcaa tttccatac tctgctcct 
      attggtggt ttcgaacgt gatctcgcc ctttttgtt 
      cggttatcg caacaaaag agtgaaacg ggagatagg 
      aaaagtgtt tggattggt tccggctaa ggccgagct 
      caccgtcca gaaccggcg gagatccac gaacgatga 
      accaatgac cagcaagga gcctttttt agggccgaa 
      ctgtaccgt acccgaagt gatagtcac gtgtaaggt 
      ggaaaacga gagaaaaag tattctgaa gcgggtgtg 
      tcagagttc gaattggac gagttacaa aaaatgtaa 
      ttttattac ggtacctga aagctgtat cagcggcga 
      ggccagagg gtcgttctc cagtgccag cacgcaaat 
      ttctaatga agactgtta gggagtttt tctagtcgc 
      gattcaagt tggcagcat tttttttta caaagagac 
      tgatgttac tggtggcca aaacttaag tatttttaa 
      gttattaac gtgtaaatt attgtattt ataaatttt 
      tgtatagcg taagagctt caataacaa atattgaaa 
      aattggcgg agtcttatt tgaatttcc cacacacgc 
      aatcaaaat caccggcaa ttggaatca attaataaa 
      atgatagca ataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY