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Gene information
General information
TC034736 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2616509..2626340
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034736
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05753

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034736 RA
      MWNKPTKLF LLLVLFSLI YSGSGQEKV AKQHPELPN 
      LARSWAQKL GGELWHFGE LVTRRTRVN ESFKRHSVI 
      KEESGETIL KAIHEKIYN MTKEKVEAV KRIMDIAEE 
      AAKAQREEE IDVNYQFVN AKNLTNSAP NYTLNDQEP 
      QEVRQDFHY DDSFITSDR RFEERYQAM VQQDELDGY 
      QQDQDQQDE EEEETDSDI EVTDGSEKD PQASQVPQT 
      DRSKLPLYR NRNFYNIPV NTNFSAVHV PSNVYERSK 
      EVISGIIWS ELLDKTFKN NYKQDPTLS WQYFGSSTG 
      FMRQFPAMI WSQEPIDLF DCRTRSWYI EAASSPKDV 
      VILVDRSGS MTGMRREIA RHVVHNILD TLGNNDYVN 
      IFTFSNTTD PLIEVLKEA MADFKTEQI ANLSLALVT 
      AFQLLENYR NESKGANCN QAIMLVTDG VQDNYMEIF 
      RDYNWDNLP FINVRVFTY LIGREVSDV RDVKWMACA 
      NRGYYVHLS TYAEVREEV LQYIPVMAR PMVLNANQK 
      PNPTWSPVY ADVTDPKLT NWLWVNRER NKQRERLLA 
      YFGRKKTLL PSDEQDKKF VHQQKHKQD NFGELQTYR 
      LMTSVSLPV YDRKPRAER VANLLGVAG TDIPIEYIE 
      SLMLPYRLG VNGYAFIVT NNGYILTHP DLRPVYQGI 
      LKPAYNRVD MTEVEVMND DSDPRSFSD QILEFRRRV 
      VMQEKGDIT LQVKSHIDE MRRLVTTTR HYFYMGVNN 
      TPFSLVIAL PAKYGFNKV EHPVENDIH RMRSNDKID 
      KSEGWLTQF FTGNWTLHP DWHYCRYLD NRYEFETPE 
      EEFLHFLRK IEGTKWKWV ECDRGLIVK VVADAKITS 
      WFSENITNS NSSKEENGD KFIRRFGIT VAFLATHSG 
      LTRWQDFPQ NVDAIAREE PSFRHFHML HNKAIDEIW 
      YRRAVEYHY VDPTAYIYS VPFDIGAEN NTLLTVSHA 
      IFREDGGKK APAAVIGYQ SYHSALYAL FRNITTSCG 
      DIPCKRTCD SDELDCFLL DDNGYIIVS DDLTHTGFF 
      FGKVRPDIM SFLVDEGIY KVTRMYDYQ GLCPEEHES 
      NNPASTFST PLDQISRLI HWATATFFY LLHTISAEL 
      ETISYPEED HEMHSYDSE HDFESETTE QPSRLTEKE 
      FDQRVLLKK TKPEPCDYE MWFYTLVHY PEKSIPSSG 
      YNKAMTDDC TRPFIVQRV FNSNMILLV VNSVCWEKE 
      NTMAKMPDP VLVDYNMSL ACYRALFNN FTRRHYMSC 
      INRNVNESE IKLCGDASV ASFSLLPLI LLTIVHLII
    CDS sequence
    • >TC034736 RA
      atgtggaac aaaccgaca aaattattt ttacttctt 
      gtattattt tccctgatt tattccgga tcaggacag 
      gaaaaagtc gccaaacaa caccccgaa ctacccaat 
      ctggccaga agctgggcg caaaaatta ggcggcgaa 
      ttgtggcat ttcggggaa ttggtcacg agaagaaca 
      agagtcaac gagagtttc aaaagacat agcgtcatt 
      aaggaggaa agtggcgag acgatcctg aaagccatc 
      catgagaaa atctacaac atgaccaaa gaaaaagtg 
      gaggctgtt aagagaatc atggatatt gcggaagaa 
      gcggcaaaa gcgcaacgc gaagaagag atagacgtg 
      aattatcaa ttcgtcaat gcgaaaaac ctgacaaat 
      tcggcccca aattacacc ctaaacgac caagaacca 
      caagaggtg agacaagac ttccattac gacgattcg 
      ttcattacg agtgatcgg cggttcgaa gagcggtac 
      caagccatg gtacagcag gacgaatta gatgggtac 
      cagcaggac caggaccaa caggacgag gaagaggag 
      gaaacggac agcgatatt gaagtcacc gatgggagc 
      gagaaggac ccccaagcc agtcaagtc ccacaaacc 
      gaccgcagc aaactgccc ctctaccgg aatcgaaat 
      ttctacaat attccagtt aacacgaat tttagcgct 
      gtccatgtc ccgagcaac gtctacgag agatcaaaa 
      gaagtgatt tcgggaatc atctggtcg gagcttttg 
      gacaaaacc ttcaaaaac aactacaaa caagacccg 
      actttatcg tggcagtac tttggcagt tccaccggt 
      ttcatgcgc caattccct gccatgatt tggtcgcaa 
      gaacccatc gacttgttc gactgtcgt accagatca 
      tggtacatc gaagctgca tcttcacca aaagatgta 
      gtaatcctg gtcgataga tctggttct atgacggga 
      atgcgacgt gaaatcgcc cgtcacgtc gtccacaat 
      attctggat actttgggg aacaacgac tatgttaac 
      attttcacg tttagtaac acaaccgat ccgctgatt 
      gaggttctg aaggaggca atggccgat ttcaagacc 
      gagcaaatt gcaaatctg tcccttgcc ttagttacg 
      gctttccag cttttggag aactaccgg aacgaaagc 
      aagggtgct aactgtaat caggccatt atgttggtc 
      acggatggg gtccaggac aactatatg gagattttc 
      cgggattat aactgggat aatctcccg tttatcaac 
      gtgcgagtg ttcacgtat ttgataggg agggaggtg 
      tccgatgtg agggacgtc aagtggatg gcgtgcgcc 
      aatcgggga tattacgtc catttgagc acttatgca 
      gaagtgagg gaggaggtc ttgcagtat atccctgtg 
      atggcaaga cccatggtt ttgaatgcc aaccaaaaa 
      ccaaacccg acctggagt cccgtttat gccgacgtg 
      actgatccc aagttgacc aactggttg tgggtcaac 
      agggagagg aataagcaa cgggagcgg cttttggcg 
      tactttggg cgcaaaaaa acgctactt ccgtccgat 
      gagcaggac aagaagttc gtccaccag caaaaacac 
      aaacaagac aacttcggg gagttgcaa acctaccgc 
      ttgatgact tccgtttcg ctgccggtg tacgacagg 
      aagccacga gcggaacgt gtcgcaaat ctgcttgga 
      gttgccgga acggacatt cccatcgaa tacatcgag 
      tccctgatg ttgccctat cggctcggt gtcaacggt 
      tacgccttt attgttacc aacaatggt tacatcctc 
      acccacccg gacctacgc cctgtgtac caagggatc 
      ctcaaaccg gcctataac cgagtcgac atgaccgaa 
      gtcgaagtc atgaacgac gacagcgac ccccggagt 
      ttcagtgac caaattctg gaattccgg cgtcgcgtg 
      gtcatgcag gagaagggc gacatcacc ttacaggtc 
      aagagccac attgacgaa atgagacga ttagtgacc 
      accacacgt cattatttc tacatgggg gtcaataac 
      acccctttt agtctagtg atagcactt ccggccaaa 
      tacggtttt aataaagtt gagcatccg gtagagaat 
      gatatacac cggatgagg tccaatgat aagattgat 
      aagagtgaa gggtggctc acacagttt ttcacgggg 
      aattggaca ctgcatcca gattggcat tattgccgc 
      tatttggac aatcgatac gaatttgag acgccggaa 
      gaggagttt ttgcacttt ttgaggaaa attgagggg 
      actaagtgg aaatgggtc gagtgcgac cgcggcctg 
      atcgtgaag gtcgtggcc gacgccaag atcacctcc 
      tggttcagc gaaaacatc accaattcc aacagcagc 
      aaggaagag aacggggat aagttcatt cgtcggttt 
      ggcatcact gtggcattc ctggcaacg cacagtgga 
      ctgacccga tggcaggac ttcccccaa aatgtggac 
      gcaatagcc agagaggaa ccgagtttt agacacttc 
      catatgctc cataataag gctattgac gaaatttgg 
      taccggagg gccgtcgag taccactat gtggacccc 
      acggcttac atatactcc gtaccgttc gatatcggg 
      gcggaaaat aacacttta ctgaccgta agtcacgca 
      atatttcgc gaagatggc ggtaagaag gcaccagcc 
      gctgtgatc gggtaccag tcgtaccac tcggccttg 
      tacgccctg ttccgcaac atcacgact agttgtggc 
      gatattccc tgcaaacgg acgtgcgat tccgacgaa 
      cttgactgt ttcttgttg gacgacaac ggttacatt 
      atcgtgagc gacgatttg actcatact ggttttttc 
      ttcgggaaa gttaggccc gatattatg agttttctg 
      gtcgatgaa gggatttat aaagtgaca cggatgtac 
      gactaccaa gggctctgt cccgaagag cacgaaagc 
      aacaacccg gcttcgact ttctcaacg cctttggac 
      caaatttct cgccttatt cactgggcc accgccact 
      ttcttctac ctccttcac acaatcagc gccgaactg 
      gagaccatc tcctaccct gaggaagac cacgagatg 
      cacagctac gattccgaa cacgatttt gaatcggaa 
      acgaccgaa cagccgtcc cgtcttacg gaaaaagaa 
      ttcgatcag cgcgttttg ctcaaaaaa accaaaccc 
      gaaccgtgc gattacgaa atgtggttt tatactcta 
      gtacattat cctgagaaa agtattccg agtagtggc 
      tataacaag gctatgaca gacgattgc acgaggccc 
      tttatagtc cagagggtt ttcaacagt aacatgatc 
      ttgttggtc gttaatagc gtttgttgg gagaaggaa 
      aatacaatg gctaaaatg ccagatccg gtcctggtt 
      gactataat atgtccttg gcgtgctat agggcgctt 
      tttaataac ttcaccagg aggcattac atgagttgc 
      atcaacaga aacgtcaac gaaagcgag atcaagctt 
      tgtggcgac gcctctgtg gcgtctttt tcgcttctt 
      cccttaatt ctcctcacc attgtgcat ctcatcatc 
      tag
    mRNA sequence
    • >TC034736 RA
      ctttattga tcaccaaca gcgaaaaag aaaaattga 
      gagcaatgc gttagcgca aaatgtgga acaaaccga 
      caaaattat ttttacttc ttgtattat tttccctga 
      tttattccg gatcaggac aggaaaaag tcgccaaac 
      aacaccccg aactaccca atctggcca gaagctggg 
      cgcaaaaat taggcggcg aattgtggc atttcgggg 
      aattggtca cgagaagaa caagagtca acgagagtt 
      tcaaaagac atagcgtca ttaaggagg aaagtggcg 
      agacgatcc tgaaagcca tccatgaga aaatctaca 
      acatgacca aagaaaaag tggaggctg ttaagagaa 
      tcatggata ttgcggaag aagcggcaa aagcgcaac 
      gcgaagaag agatagacg tgaattatc aattcgtca 
      atgcgaaaa acctgacaa attcggccc caaattaca 
      ccctaaacg accaagaac cacaagagg tgagacaag 
      acttccatt acgacgatt cgttcatta cgagtgatc 
      ggcggttcg aagagcggt accaagcca tggtacagc 
      aggacgaat tagatgggt accagcagg accaggacc 
      aacaggacg aggaagagg aggaaacgg acagcgata 
      ttgaagtca ccgatggga gcgagaagg acccccaag 
      ccagtcaag tcccacaaa ccgaccgca gcaaactgc 
      ccctctacc ggaatcgaa atttctaca atattccag 
      ttaacacga attttagcg ctgtccatg tcccgagca 
      acgtctacg agagatcaa aagaagtga tttcgggaa 
      tcatctggt cggagcttt tggacaaaa ccttcaaaa 
      acaactaca aacaagacc cgactttat cgtggcagt 
      actttggca gttccaccg gtttcatgc gccaattcc 
      ctgccatga tttggtcgc aagaaccca tcgacttgt 
      tcgactgtc gtaccagat catggtaca tcgaagctg 
      catcttcac caaaagatg tagtaatcc tggtcgata 
      gatctggtt ctatgacgg gaatgcgac gtgaaatcg 
      cccgtcacg tcgtccaca atattctgg atactttgg 
      ggaacaacg actatgtta acattttca cgtttagta 
      acacaaccg atccgctga ttgaggttc tgaaggagg 
      caatggccg atttcaaga ccgagcaaa ttgcaaatc 
      tgtcccttg ccttagtta cggctttcc agcttttgg 
      agaactacc ggaacgaaa gcaagggtg ctaactgta 
      atcaggcca ttatgttgg tcacggatg gggtccagg 
      acaactata tggagattt tccgggatt ataactggg 
      ataatctcc cgtttatca acgtgcgag tgttcacgt 
      atttgatag ggagggagg tgtccgatg tgagggacg 
      tcaagtgga tggcgtgcg ccaatcggg gatattacg 
      tccatttga gcacttatg cagaagtga gggaggagg 
      tcttgcagt atatccctg tgatggcaa gacccatgg 
      ttttgaatg ccaaccaaa aaccaaacc cgacctgga 
      gtcccgttt atgccgacg tgactgatc ccaagttga 
      ccaactggt tgtgggtca acagggaga ggaataagc 
      aacgggagc ggcttttgg cgtactttg ggcgcaaaa 
      aaacgctac ttccgtccg atgagcagg acaagaagt 
      tcgtccacc agcaaaaac acaaacaag acaacttcg 
      gggagttgc aaacctacc gcttgatga cttccgttt 
      cgctgccgg tgtacgaca ggaagccac gagcggaac 
      gtgtcgcaa atctgcttg gagttgccg gaacggaca 
      ttcccatcg aatacatcg agtccctga tgttgccct 
      atcggctcg gtgtcaacg gttacgcct ttattgtta 
      ccaacaatg gttacatcc tcacccacc cggacctac 
      gccctgtgt accaaggga tcctcaaac cggcctata 
      accgagtcg acatgaccg aagtcgaag tcatgaacg 
      acgacagcg acccccgga gtttcagtg accaaattc 
      tggaattcc ggcgtcgcg tggtcatgc aggagaagg 
      gcgacatca ccttacagg tcaagagcc acattgacg 
      aaatgagac gattagtga ccaccacac gtcattatt 
      tctacatgg gggtcaata acacccctt ttagtctag 
      tgatagcac ttccggcca aatacggtt ttaataaag 
      ttgagcatc cggtagaga atgatatac accggatga 
      ggtccaatg ataagattg ataagagtg aagggtggc 
      tcacacagt ttttcacgg ggaattgga cactgcatc 
      cagattggc attattgcc gctatttgg acaatcgat 
      acgaatttg agacgccgg aagaggagt ttttgcact 
      ttttgagga aaattgagg ggactaagt ggaaatggg 
      tcgagtgcg accgcggcc tgatcgtga aggtcgtgg 
      ccgacgcca agatcacct cctggttca gcgaaaaca 
      tcaccaatt ccaacagca gcaaggaag agaacgggg 
      ataagttca ttcgtcggt ttggcatca ctgtggcat 
      tcctggcaa cgcacagtg gactgaccc gatggcagg 
      acttccccc aaaatgtgg acgcaatag ccagagagg 
      aaccgagtt ttagacact tccatatgc tccataata 
      aggctattg acgaaattt ggtaccgga gggccgtcg 
      agtaccact atgtggacc ccacggctt acatatact 
      ccgtaccgt tcgatatcg gggcggaaa ataacactt 
      tactgaccg taagtcacg caatatttc gcgaagatg 
      gcggtaaga aggcaccag ccgctgtga tcgggtacc 
      agtcgtacc actcggcct tgtacgccc tgttccgca 
      acatcacga ctagttgtg gcgatattc cctgcaaac 
      ggacgtgcg attccgacg aacttgact gtttcttgt 
      tggacgaca acggttaca ttatcgtga gcgacgatt 
      tgactcata ctggttttt tcttcggga aagttaggc 
      ccgatatta tgagttttc tggtcgatg aagggattt 
      ataaagtga cacggatgt acgactacc aagggctct 
      gtcccgaag agcacgaaa gcaacaacc cggcttcga 
      ctttctcaa cgcctttgg accaaattt ctcgcctta 
      ttcactggg ccaccgcca ctttcttct acctccttc 
      acacaatca gcgccgaac tggagacca tctcctacc 
      ctgaggaag accacgaga tgcacagct acgattccg 
      aacacgatt ttgaatcgg aaacgaccg aacagccgt 
      cccgtctta cggaaaaag aattcgatc agcgcgttt 
      tgctcaaaa aaaccaaac ccgaaccgt gcgattacg 
      aaatgtggt tttatactc tagtacatt atcctgaga 
      aaagtattc cgagtagtg gctataaca aggctatga 
      cagacgatt gcacgaggc cctttatag tccagaggg 
      ttttcaaca gtaacatga tcttgttgg tcgttaata 
      gcgtttgtt gggagaagg aaaatacaa tggctaaaa 
      tgccagatc cggtcctgg ttgactata atatgtcct 
      tggcgtgct atagggcgc tttttaata acttcacca 
      ggaggcatt acatgagtt gcatcaaca gaaacgtca 
      acgaaagcg agatcaagc tttgtggcg acgcctctg 
      tggcgtctt tttcgcttc ttcccttaa ttctcctca 
      ccattgtgc atctcatca tctagatcg gttctagat 
      attatgaaa tatctgtga taaactcga catttatat 
      attgttcgc aaccatatc gagttttgc cattatttg 
      ttctttgtc attatatat tctatatgt aatgctcaa 
      taaatactt tttaatagg
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