Status
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Gene information
General information
TC033398
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015112
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3101771..3105374
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033398
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05752

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033398 RA
      MGTVHAKSE ENAPILPTI PQEHIKSEP AQHQDQREI 
      FLDGVKARV DKIHVDGLA RTKDDIIED CIRDLFKAA 
      DFQDVLLRA HRARLKLDE LGCFKNIAV FIDTSKGQG 
      ASPDGLEVT FNVTEHKRV TGGVTTQVG NNEGALLIG 
      LRAPNLFGR GERVQLEYS HGSKRTTNF NVAFIKPMR 
      GKYRPMFTT SVFQSNAEW PVSGYKQLD RGLLFDFGF 
      HSTALIKHN IQWEAAIRD LAVLSRNTS FEVREQAGL 
      SLKSALRHI ISIDLRDDL IFPTSGSLL QLTSEVAGL 
      GGDVGFLKN EFFIQGNYS LVEDFVLQG TLSGGFMRG 
      LSNDMKIGM SDMYYLGGP LTLRGFQMR GVGPHSEGD 
      ALGSMAYWA SGLHLFSPL PFRPGRGGF GDLFKTHLF 
      ITAGNVGDF SLSDKNLID SLTASVRIS YGVGIALRL 
      GNMARVEVN YCFPHTFEK GDQIHPGIQ FGIGVQFV
    CDS sequence
    • >TC033398 RA
      atgggcaca gtacacgca aaatcggaa gaaaatgcc 
      ccaattctg cctacgatc ccccaagaa cacattaaa 
      tcagaacct gcacaacac caagaccag cgcgagatt 
      tttctagac ggggttaag gcccgtgtt gataaaatt 
      cacgtggat ggactggcc cggactaag gacgatata 
      atcgaagac tgcatcagg gatttattt aaagcggca 
      gatttccaa gacgtgctc ctcagggcg caccgggcg 
      cgccttaaa cttgatgag ttgggatgt ttcaagaat 
      attgcagtt tttatcgat acgagtaag ggacaaggt 
      gcgagccct gacggactt gaagttact tttaacgtt 
      actgaacat aaaagggtg acgggaggg gtcaccacc 
      caagtgggg aacaacgaa ggggcgctg cttatcggc 
      ttaagggct cctaattta tttggacgt ggggagaga 
      gtgcaactt gaatacagt catgggtcc aagaggacg 
      actaatttt aatgttgct ttcatcaaa ccgatgagg 
      ggcaaatat aggcccatg tttaccacc agtgtgttc 
      caatcaaac gctgaatgg cctgtatcc ggttacaaa 
      caactggat agaggttta ctttttgac tttggattc 
      cactcaact gccttgatc aaacataac attcagtgg 
      gaagccgcc atccgtgat ttggctgta ttgagcaga 
      aatacttct tttgaagtt agagaacaa gccggactt 
      agtctaaaa tcggcattg agacacata atatcaatt 
      gatttaaga gatgatttg atttttccc acatccggt 
      agtctctta cagctaaca tcagaagtc gcaggactg 
      ggtggagat gttggcttt ttaaaaaat gaatttttt 
      attcaaggg aattattcg cttgttgag gattttgtt 
      ttacaagga acattgtct ggtggtttc atgcgaggt 
      ttaagtaac gatatgaaa attggaatg tccgatatg 
      tattatctt gggggccct ttaacgttg cgcggtttt 
      caaatgcga ggtgtgggt ccccattca gagggcgac 
      gctttgggc tcgatggcc tattgggcg tcaggattg 
      catttgttt agtcctttg ccgtttaga cctggccgt 
      ggaggattt ggtgatttg tttaaaacg caccttttt 
      atcactgca ggaaatgtg ggggatttc agcttgagc 
      gataaaaac ctgatagac tcgctcacg gcaagcgtg 
      cgcatatca tacggcgtg ggaatcgcc cttcgtctc 
      ggaaacatg gcccgagtt gaagtgaac tactgtttt 
      ccccacacg tttgaaaaa ggcgaccaa atccatcca 
      ggaatccaa ttcggaatc ggcgtacaa ttcgtgtaa
    mRNA sequence
    • >TC033398 RA
      cttaattgt tgaatatta tcagtaaaa gcgatattt 
      acgattccc agacaattt tcctcacaa aaagttggg 
      gaattcaag ctttgtttt ttccccagt gagcaatct 
      gtcatctgt caacatcat ttaattttc cgaaaaaca 
      gtatttttt catgaaaaa tcatccaat tgctaaatg 
      aaatgctac attaacaaa aaaactcgt attaacaca 
      tgcagctat gggcacagt acacgcaaa atcggaaga 
      aaatgcccc aattctgcc tacgatccc ccaagaaca 
      cattaaatc agaacctgc acaacacca agaccagcg 
      cgagatttt tctagacgg ggttaaggc ccgtgttga 
      taaaattca cgtggatgg actggcccg gactaagga 
      cgatataat cgaagactg catcaggga tttatttaa 
      agcggcaga tttccaaga cgtgctcct cagggcgca 
      ccgggcgcg ccttaaact tgatgagtt gggatgttt 
      caagaatat tgcagtttt tatcgatac gagtaaggg 
      acaaggtgc gagccctga cggacttga agttacttt 
      taacgttac tgaacataa aagggtgac gggaggggt 
      caccaccca agtggggaa caacgaagg ggcgctgct 
      tatcggctt aagggctcc taatttatt tggacgtgg 
      ggagagagt gcaacttga atacagtca tgggtccaa 
      gaggacgac taattttaa tgttgcttt catcaaacc 
      gatgagggg caaatatag gcccatgtt taccaccag 
      tgtgttcca atcaaacgc tgaatggcc tgtatccgg 
      ttacaaaca actggatag aggtttact ttttgactt 
      tggattcca ctcaactgc cttgatcaa acataacat 
      tcagtggga agccgccat ccgtgattt ggctgtatt 
      gagcagaaa tacttcttt tgaagttag agaacaagc 
      cggacttag tctaaaatc ggcattgag acacataat 
      atcaattga tttaagaga tgatttgat ttttcccac 
      atccggtag tctcttaca gctaacatc agaagtcgc 
      aggactggg tggagatgt tggcttttt aaaaaatga 
      attttttat tcaagggaa ttattcgct tgttgagga 
      ttttgtttt acaaggaac attgtctgg tggtttcat 
      gcgaggttt aagtaacga tatgaaaat tggaatgtc 
      cgatatgta ttatcttgg gggcccttt aacgttgcg 
      cggttttca aatgcgagg tgtgggtcc ccattcaga 
      gggcgacgc tttgggctc gatggccta ttgggcgtc 
      aggattgca tttgtttag tcctttgcc gtttagacc 
      tggccgtgg aggatttgg tgatttgtt taaaacgca 
      cctttttat cactgcagg aaatgtggg ggatttcag 
      cttgagcga taaaaacct gatagactc gctcacggc 
      aagcgtgcg catatcata cggcgtggg aatcgccct 
      tcgtctcgg aaacatggc ccgagttga agtgaacta 
      ctgttttcc ccacacgtt tgaaaaagg cgaccaaat 
      ccatccagg aatccaatt cggaatcgg cgtacaatt 
      cgtgtaaaa tccaaatca tcatagtca tttatttcg 
      ttgaaacta aataaataa tgtacactg ttattgaaa 
      taaagttgt taacactga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY