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Gene information
General information
TC015109 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3181113..3185543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015109
    iBeetle screen iB_05751

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature partially decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Many un-hatched embryos observed in the non-squeezed slide.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015109 RA
      MYHIFLVLF YAQTCLGGP PKVENKPML LDSFIPASM 
QIIAHVTEL MKFDLKPTS GSSTTTTTT MRPTRPHKP 
GIYAPEAPL GPEAYFAKG FSYEEYVER LKYPTGYNY 
FDRYKFPVE EEGRSLGDD VPQEIDLVV GKRLGDLLR 
LANTIEDVT EVEDRDRQV QDFTNLYDD SDFRVLLLR 
QKKKPPTRA YVSLLSLYD LLNKESKKL GLNKYSGYS 
QKILTELAE ISTGTSSDQ LRAVLSKVV QRRDTTNPS 
IVKRINDLI KDLEESSSY INAALRYIP PLPFVL
    CDS sequence
    • >TC015109 RA
      atgtaccac attttcttg gtgttgttc tacgctcag 
acatgtctg ggaggaccg ccaaaggtt gaaaataag 
cccatgtta ttggactcg tttatccct gcgagtatg 
cagatcatt gcacacgtt accgaactt atgaaattt 
gatctcaaa cctacaagt ggttcctcc accactacc 
acaacgaca atgcgacct acaagacct cacaagccc 
ggaatttac gctccggaa gcgccacta ggtccggaa 
gcgtacttt gccaaaggg ttctcctat gaggagtat 
gtggaaagg ctcaagtac cccaccggg tacaactac 
tttgacaga tacaagttt ccagtggag gaagaagga 
agaagtctt ggcgacgac gtacctcaa gaaatcgat 
ctagttgta ggtaaaaga ttaggtgat ttattaaga 
ctggctaac actattgaa gacgtaacc gaagttgaa 
gatcgtgat cgacaagta caagatttt acgaacttg 
tacgacgat tcagatttt agagtttta cttctgaga 
caaaagaaa aaaccacca actagagcc tatgtctcg 
cttttatca ctttatgac cttttgaac aaagagtcc 
aaaaaacta ggtctaaac aaatacagc ggttactct 
cagaaaatc ttgactgag cttgcggaa atctccaca 
ggaacatcc tctgatcaa cttcgtgcc gtcctttca 
aaggtggtt caaagaaga gacacaact aatccttca 
attgttaaa cgaatcaat gatctcatc aaagacttg 
gaagaaagt agcagttac ataaacgct gcgctgcga 
tatatccct cctttaccc tttgtttta taa
    mRNA sequence
    • >TC015109 RA
      ttaaacaag acagtatgc ttctgagtg tctgcacac 
taaaatgta ccacatttt cttggtgtt gttctacgc 
tcagacatg tctgggagg accgccaaa ggttgaaaa 
taagcccat gttattgga ctcgtttat ccctgcgag 
tatgcagat cattgcaca cgttaccga acttatgaa 
atttgatct caaacctac aagtggttc ctccaccac 
taccacaac gacaatgcg acctacaag acctcacaa 
gcccggaat ttacgctcc ggaagcgcc actaggtcc 
ggaagcgta ctttgccaa agggttctc ctatgagga 
gtatgtgga aaggctcaa gtaccccac cgggtacaa 
ctactttga cagatacaa gtttccagt ggaggaaga 
aggaagaag tcttggcga cgacgtacc tcaagaaat 
cgatctagt tgtaggtaa aagattagg tgatttatt 
aagactggc taacactat tgaagacgt aaccgaagt 
tgaagatcg tgatcgaca agtacaaga ttttacgaa 
cttgtacga cgattcaga ttttagagt tttacttct 
gagacaaaa gaaaaaacc accaactag agcctatgt 
ctcgctttt atcacttta tgacctttt gaacaaaga 
gtccaaaaa actaggtct aaacaaata cagcggtta 
ctctcagaa aatcttgac tgagcttgc ggaaatctc 
cacaggaac atcctctga tcaacttcg tgccgtcct 
ttcaaaggt ggttcaaag aagagacac aactaatcc 
ttcaattgt taaacgaat caatgatct catcaaaga 
cttggaaga aagtagcag ttacataaa cgctgcgct 
gcgatatat ccctccttt accctttgt tttataaaa 
aaattgtat agaatgaaa tgatcgaac attggctaa 
attaattgt aaaaaaatg tacattgta aaattactg 
taaaaaaat aaattatct tgccgaaat gtgatggct 
gttttgact gcctggctg gtacaattt tgtgaatat 
gtatcataa tagcgcgga aaatggaaa ttaataaaa 
gcaatatca atgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY