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Gene information
General information
TC015083 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3528408..3529444
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015083
    iBeetle screen iB_05749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015083 RA
      MNLLIFLTV AIVFASAKP ALKIGFHPN PHIINGDAA 
      AKGQFPYQI SYQFGILGV HEHVCGGSI LSPTFILTA 
      GHCVTEVPT QGDHRIVAG ITELNEQNN QRQEIQVVE 
      KIVHPDFNG RVGPNDIAL LKLATPLEF GDLVQPIGL 
      PEPGSEFTG DSVLTGWGS TSTTIVPIL PNHLQTVTV 
      PILSYDDCK KAVDALLDE NQENPLSEV SNVCTGPLS 
      KGESACLGD SGGPLAQNG TIVGVVSWG FVPCSHPEA 
      PSVYTKVSS FVDFIKEHV HDLPQ
    CDS sequence
    • >TC015083 RA
      atgaaccta ctcatcttt ctaacagtt gcgattgtc 
      ttcgcttcg gccaaaccc gccctaaaa atcggcttc 
      caccccaac ccccacata atcaacgga gacgccgcc 
      gccaaaggc caattcccc taccaaatc tcctaccag 
      tttggaatc ttgggcgtc cacgagcac gtctgtgga 
      ggctccatc ctctcgccc accttcatc ctcacagcg 
      gggcactgc gtcaccgaa gtgccaaca cagggggac 
      caccgaatc gtcgctgga atcaccgaa ctcaacgaa 
      caaaacaac caaagacaa gagatccaa gtcgttgaa 
      aaaatcgtc catcctgac ttcaacgga cgcgtggga 
      ccaaacgac atcgcttta ctcaaatta gcaacaccg 
      ctagaattc ggagacctg gtacaacct atcgggttg 
      cctgaaccc ggctctgaa ttcacagga gattcagtt 
      ctgacagga tggggctcc acctccacc accattgtc 
      ccaattttg cccaaccat ttgcaaact gtcacggtg 
      cccatcttg tcttacgac gattgtaag aaagcagtc 
      gatgcttta cttgacgaa aatcaggaa aatccccta 
      agtgaggtg tcaaatgtt tgcactggg cctttgtca 
      aagggcgaa agtgcctgt ttgggggac agtgggggg 
      ccattggcc cagaatggt accatcgtg ggggttgtt 
      tcctggggt tttgtacca tgtagccat ccagaggca 
      ccgtcagtt tacaccaaa gttagcagt tttgtcgat 
      tttatcaaa gaacatgtc cacgatttg ccccaataa
    mRNA sequence
    • >TC015083 RA
      cacaaccaa aatgaacct actcatctt tctaacagt 
      tgcgattgt cttcgcttc ggccaaacc cgccctaaa 
      aatcggctt ccaccccaa cccccacat aatcaacgg 
      agacgccgc cgccaaagg ccaattccc ctaccaaat 
      ctcctacca gtttggaat cttgggcgt ccacgagca 
      cgtctgtgg aggctccat cctctcgcc caccttcat 
      cctcacagc ggggcactg cgtcaccga agtgccaac 
      acaggggga ccaccgaat cgtcgctgg aatcaccga 
      actcaacga acaaaacaa ccaaagaca agagatcca 
      agtcgttga aaaaatcgt ccatcctga cttcaacgg 
      acgcgtggg accaaacga catcgcttt actcaaatt 
      agcaacacc gctagaatt cggagacct ggtacaacc 
      tatcgggtt gcctgaacc cggctctga attcacagg 
      agattcagt tctgacagg atggggctc cacctccac 
      caccattgt cccaatttt gcccaacca tttgcaaac 
      tgtcacggt gcccatctt gtcttacga cgattgtaa 
      gaaagcagt cgatgcttt acttgacga aaatcagga 
      aaatcccct aagtgaggt gtcaaatgt ttgcactgg 
      gcctttgtc aaagggcga aagtgcctg tttggggga 
      cagtggggg gccattggc ccagaatgg taccatcgt 
      gggggttgt ttcctgggg ttttgtacc atgtagcca 
      tccagaggc accgtcagt ttacaccaa agttagcag 
      ttttgtcga ttttatcaa agaacatgt ccacgattt 
      gccccaata aatgtgtga agtaaaaaa aaaatcgtg 
      ttttttttg ttctctttt agattaagg tgttatgca 
      ttttgacgt tttcttaaa taaaacctt aaataaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY