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Gene information
General information
TC015075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3725725..3726754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015075
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    iBeetle screen iB_05747

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015075 RA
      MEISKVVLY NCARKLEPL NCEFCTFTS DFKLLLHRH 
      TRSLHHNVD ISRHKLSEE IKWFQCEQC AFKTKVSSN 
      LQRHITRKH TGCHQFSCD SCSYKTQNK KNFTKHKNY 
      NHSQDVVMY ECTECPYKT KKKFSLKCH IIRRHTESE 
      AIIEWYNCD RCTYRSKVQ SDLKKHTLV VHFTDSELK 
      WFECDLCDY KGRFKANLN KHIKRNHGG ASVVFSCDL 
      CDYKSEKKF EIKRHKYSA HLTKKRSTR GSGEIFRCE 
      LCEYKCKRK DNFRRHKLS LHSVKQEVS
    CDS sequence
    • >TC015075 RA
      atggaaata tccaaagtt gtactatac aactgtgct 
      cgaaaactt gagcctttg aattgtgaa ttttgcact 
      tttacaagc gactttaag cttcttttg catcgccac 
      acccgaagt ttgcatcac aatgttgac atcagcaga 
      cacaaacta tctgaagaa ataaagtgg tttcagtgt 
      gaacaatgt gctttcaag actaaggtc agctcgaac 
      ctacaaaga cacatcacg agaaaacac actggatgc 
      catcaattc agttgtgac tcatgcagt tacaaaacg 
      caaaacaaa aagaatttc acaaaacac aaaaactat 
      aatcactcg caagacgtg gtcatgtat gaatgtaca 
      gaatgtcca tacaaaacc aagaaaaaa ttcagcctg 
      aaatgccac ataattagg agacacacg gagtcagaa 
      gccattatt gagtggtac aactgtgac cgctgtacc 
      tatcgttca aaggtgcaa agtgactta aagaaacat 
      acactggtt gtgcatttc acagactcg gaacttaaa 
      tggtttgaa tgcgatctt tgcgattat aaaggaaga 
      ttcaaagct aatttgaat aaacatatt aagaggaat 
      catggaggt gctagtgtg gtctttagt tgcgatttg 
      tgtgactac aaatcggag aaaaaattc gagattaaa 
      agacataag tatagtgca catttgacg aaaaagaga 
      agtacacgt ggaagtggt gagattttt aggtgcgag 
      ttgtgcgag tataagtgt aagagaaaa gataatttt 
      agaaggcat aagttgagc ttgcattct gtaaaacaa 
      gaagtatcg tga
    mRNA sequence
    • >TC015075 RA
      cctgtcaaa taatggcac aatacaaag taaataaat 
      aaaaacatt ccaaaagtt gattattct aataaaaaa 
      caaacttag agtaataaa taagtaacg atttgggat 
      tcaattatg gaaatatcc aaagttgta ctatacaac 
      tgtgctcga aaacttgag cctttgaat tgtgaattt 
      tgcactttt acaagcgac tttaagctt cttttgcat 
      cgccacacc cgaagtttg catcacaat gttgacatc 
      agcagacac aaactatct gaagaaata aagtggttt 
      cagtgtgaa caatgtgct ttcaagact aaggtcagc 
      tcgaaccta caaagacac atcacgaga aaacacact 
      ggatgccat caattcagt tgtgactca tgcagttac 
      aaaacgcaa aacaaaaag aatttcaca aaacacaaa 
      aactataat cactcgcaa gacgtggtc atgtatgaa 
      tgtacagaa tgtccatac aaaaccaag aaaaaattc 
      agcctgaaa tgccacata attaggaga cacacggag 
      tcagaagcc attattgag tggtacaac tgtgaccgc 
      tgtacctat cgttcaaag gtgcaaagt gacttaaag 
      aaacataca ctggttgtg catttcaca gactcggaa 
      cttaaatgg tttgaatgc gatctttgc gattataaa 
      ggaagattc aaagctaat ttgaataaa catattaag 
      aggaatcat ggaggtgct agtgtggtc tttagttgc 
      gatttgtgt gactacaaa tcggagaaa aaattcgag 
      attaaaaga cataagtat agtgcacat ttgacgaaa 
      aagagaagt acacgtgga agtggtgag atttttagg 
      tgcgagttg tgcgagtat aagtgtaag agaaaagat 
      aattttaga aggcataag ttgagcttg cattctgta 
      aaacaagaa gtatcgtga attgttata ataaactgt 
      ttttgtttg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY