Status
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Gene information
General information
TC015062 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3901955..3906382
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015062
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05746

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015062 RA
        MDLTPPKDS KGPFSHAST PLTHFPRKR KHDDTFESP 
        KSPPFSSTL NESLITDLK DICLVGSVP NTPEKQLSF 
        LAQTVVSSP IQESRKILY GSIKVVKPE RDRRCSFRN 
        KYLPVNNQK LLQRILNIN VIMDVIFRY LSGGDLYRF 
        SLVSPSFCK ALEASKDAF LRYQVYKDS YKDNKENYV 
        ITPPGSPDK PDSPPRTSS VTFNKFLRA ASRLKGTQT 
        LRKCDRCSG VAVVDHQIS QCQNVHSCG LIRCLLCSS 
        FSETGHKDF KDACGHASF RPSLGNMSN TMEDSSSNF 
        SSQDSGFYS ENESPVKVK RILFQNVDK QTSNGLSLW 
        NKNQVVVTY KKNDSLSYI PVVANDTPR REAAGDSPP 
        SPQRHTYAV GSKQSKRNL KRLTR
      CDS sequence
      • >TC015062 RA
        atggacctg acaccgccg aaagactcg aaggggccc 
        ttcagccac gcgtcaacc cccctgact cacttcccc 
        cgcaagcgc aaacatgac gacactttt gagtccccg 
        aagagcccc ccgttttcc agcaccttg aacgagtcg 
        ctaatcacc gatttgaaa gacatttgc ttggttggt 
        tccgttcct aatactccc gaaaaacag ttgagtttt 
        ttggcgcaa actgttgtc tcgtccccg attcaagag 
        tcgaggaaa attctttat ggtagtatt aaagtggtt 
        aaacccgag cgtgataga aggtgtagc tttcgtaat 
        aagtatctg cccgtcaat aatcagaaa ttgttgcaa 
        cgtatcctc aacatcaat gttataatg gacgtaatt 
        tttcggtac ttatcaggg ggcgacttg tacaggttc 
        agtcttgtc tcaccttcg ttttgtaaa gccttggag 
        gcgagtaaa gacgcgttt ttgcggtat caggtctac 
        aaagacagt tataaagac aacaaggag aattatgtg 
        attacgccg ccaggatcg ccagacaag cctgacagc 
        cccccgagg actagtagc gttactttc aacaagttt 
        ctcagggcg gccagcagg ctcaaaggc acccaaacc 
        ctgcgtaaa tgtgacaga tgcagtggg gttgcagtc 
        gtcgaccac caaatcagc cagtgccag aacgtgcac 
        tcttgcggc ctcattcgc tgcctcctg tgttcaagc 
        ttctccgag acgggccat aaagacttc aaggacgcc 
        tgtggccac gcctcattt cgcccctct ttgggcaac 
        atgagcaac acgatggaa gactcctct tcgaatttt 
        tcgagtcaa gactcgggt ttttactcg gaaaatgaa 
        tcgcccgta aaggtgaaa cggatcctc ttccaaaac 
        gttgataaa cagacgtcc aatggcctg tctttatgg 
        aacaaaaac caagtcgtt gtgacgtac aagaagaac 
        gacagcctc tcgtacatt ccggtcgtg gccaacgac 
        accccccgc agagaggcg gcgggcgac agccccccg 
        agcccccag agacacacc tacgcggtc ggctccaag 
        caaagcaag aggaatttg aagcgacta acgcgttag
      mRNA sequence
      • >TC015062 RA
        gacgaattt tatcaatta ttttgcctg gggttaaat 
        atttgacgc gttgtgctt gatttggtg ctaattttt 
        ggttgaaat tccaatttt gcgtgaatt tgttgtcag 
        tttttaaat ttggcgcgt ccagcgcag cgttgacag 
        gtgttcgag gtcggtgaa agcacaaaa agtgcattt 
        atctcgttt ttttcgtgt gtcctaatg ttataccca 
        cgtccgcgt gttatttta atgtggttt taatcaata 
        cgtaacaag acagccccc caagtcccc gtttttatc 
        cactaacga aatggcgcc atagttgtt tgtacacat 
        ccgttcctt ttaataaat aaagatgga cctgacacc 
        gccgaaaga ctcgaaggg gcccttcag ccacgcgtc 
        aacccccct gactcactt cccccgcaa gcgcaaaca 
        tgacgacac ttttgagtc cccgaagag ccccccgtt 
        ttccagcac cttgaacga gtcgctaat caccgattt 
        gaaagacat ttgcttggt tggttccgt tcctaatac 
        tcccgaaaa acagttgag ttttttggc gcaaactgt 
        tgtctcgtc cccgattca agagtcgag gaaaattct 
        ttatggtag tattaaagt ggttaaacc cgagcgtga 
        tagaaggtg tagctttcg taataagta tctgcccgt 
        caataatca gaaattgtt gcaacgtat cctcaacat 
        caatgttat aatggacgt aatttttcg gtacttatc 
        agggggcga cttgtacag gttcagtct tgtctcacc 
        ttcgttttg taaagcctt ggaggcgag taaagacgc 
        gtttttgcg gtatcaggt ctacaaaga cagttataa 
        agacaacaa ggagaatta tgtgattac gccgccagg 
        atcgccaga caagcctga cagcccccc gaggactag 
        tagcgttac tttcaacaa gtttctcag ggcggccag 
        caggctcaa aggcaccca aaccctgcg taaatgtga 
        cagatgcag tggggttgc agtcgtcga ccaccaaat 
        cagccagtg ccagaacgt gcactcttg cggcctcat 
        tcgctgcct cctgtgttc aagcttctc cgagacggg 
        ccataaaga cttcaagga cgcctgtgg ccacgcctc 
        atttcgccc ctctttggg caacatgag caacacgat 
        ggaagactc ctcttcgaa tttttcgag tcaagactc 
        gggttttta ctcggaaaa tgaatcgcc cgtaaaggt 
        gaaacggat cctcttcca aaacgttga taaacagac 
        gtccaatgg cctgtcttt atggaacaa aaaccaagt 
        cgttgtgac gtacaagaa gaacgacag cctctcgta 
        cattccggt cgtggccaa cgacacccc ccgcagaga 
        ggcggcggg cgacagccc cccgagccc ccagagaca 
        cacctacgc ggtcggctc caagcaaag caagaggaa 
        tttgaagcg actaacgcg ttagcatca cgtgaccga 
        ttatgtgat aagttttgt attttttag tgtctttta 
        acagttcca gtgttatgt aatgtaggt taagcattt 
        ttcataaag ttgatacaa tttctcatt tgttttatt 
        gggggttgg tagcaatgc gcatgcgcc ggttcccga 
        cccgagcgc gtgcagaat tttgaatca gtttttaca 
        gagcacggt tggagatgg tattcccag ggttgagta 
        ccgtctttg aggctcttt gtgtggttt gccccccga 
        ttgcgtgac ctgttagtg cccccttgt ggcaaacaa 
        tcgcgaatc gtggttgcc atggtgatg ttatttgac 
        agttacctt tgttgattt attgacaga tgaattaat 
        ttttttccc tgttgtgtt ttttttaca cgttccaga 
        gtgttgatt cgagtgcat gtttgtggt aattggaga 
        ggttttcgc aaaggtgag tcaggaatt gtgagaaat 
        ttgattatt ttcgttttt catgttgcc aaccgttac 
        gtcacgaaa tggtgcatt ttcattggt cgacgccat 
        tttatcgat ggctacgtt ttgtagtga aaacgactt 
        tagttttta agaatttct gtgttcatt tctctaaat 
        tcactcatg tttcttcat aaatttacg gtgttgttg 
        ccacaagta agtttgcat gg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY