Status
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Gene information
General information
TC015026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4498066..4509253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015026
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05743

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    Comment: about 50%
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015026 RA
      MLKKEIWCL RDEYDKLDK LLKEKEYEF SSSSTTCST 
      SDSESCSTC SEESEEVET SQNLENVQK TNMKNLHSE 
      FDHLSVVPE ENSTENSDK SSLQENIIK PDQTYEKSN 
      TLPNLQPKN FFSPITISK SDDEFYTEK VVTTNASNF 
      YQNMVLVPK SEPSVVPSM GKTTTDLLV KTDNHPFDG 
      TPSRSTFSN GGNLEELLN DIESISQDI LKITNDQAA 
      ENSTQKPYK SELNVVLMP NPMPLLGFE KYKNLQNSF 
      ESLHSKSAE KLNNCDQTN PFFAEQNSD FFNSRYNPD 
      NFQKGSKTN LLELTNSSD LISSENEEN LKFQTSKTE 
      NTEEKKSPK ESFRRKVSI HFKGKKEKK NSIFDIKFG 
      ASEPKHQKT PSVESRKTP EPKTSSSGS EKKKPEEKK 
      ARKSVSVSP DRKHVHVKD EKKDNDQKR HRKHRKSRR 
      GTLTSVERP HRERSFSVC TDRSEHRFG APFEDSERE 
      RTNSLSSCE TVKTRKMSN ISNIPLNGK VPWCACWGN 
      GCI
    CDS sequence
    • >TC015026 RA
      atgctcaaa aaagaaatc tggtgtttg cgtgacgaa 
      tacgacaaa ctcgacaaa ctcctcaag gaaaaagaa 
      tacgaattt tcctcctcc tcaaccact tgtagtacc 
      agcgattct gaaagttgt agcacatgc agtgaagaa 
      tcggaagaa gttgaaacg tcgcaaaac ctcgaaaac 
      gtccaaaaa accaacatg aaaaacctc cactcggaa 
      ttcgaccat ttatcggtc gttccggaa gaaaacagt 
      actgaaaat tccgacaaa agctcgctt caagaaaat 
      attattaaa ccagaccag acttacgaa aaatcaaac 
      actttaccg aacctccaa ccgaaaaat tttttctcg 
      cccattacg ataagtaaa agtgatgat gaattttac 
      accgagaaa gtggtgacc actaatgcg agcaatttt 
      taccagaac atggttcta gtgccgaaa agtgaacca 
      tcggtggta ccatcaatg ggcaaaacc acgactgat 
      ttattggta aaaactgac aatcacccc tttgatggt 
      acccccagt aggagcact ttcagcaat ggagggaat 
      ttagaagaa ctacttaat gatattgaa tcaatttct 
      caggatatt ttaaaaatt accaacgac caagctgcc 
      gaaaatagc actcaaaaa ccgtacaaa agtgaacta 
      aacgttgtc ctaatgcca aacccgatg ccactgctc 
      ggtttcgaa aaatacaaa aacttacaa aacagtttc 
      gaaagttta cactcgaaa tcggcggaa aaactcaac 
      aattgtgat caaacaaat ccatttttc gccgaacaa 
      aattcggat tttttcaat tcgcgttac aaccctgac 
      aattttcaa aaaggctca aaaactaat cttctagaa 
      ctgaccaac tcgtccgat ttgatttcg agcgaaaat 
      gaggaaaat cttaaattt caaacgtca aagaccgaa 
      aacactgag gagaaaaaa tcaccgaaa gagagtttt 
      cgtaggaag gtttccatc cattttaag ggtaaaaaa 
      gaaaagaaa aactcaatt tttgatata aaatttggt 
      gcttctgaa ccgaaacat caaaaaacg ccaagtgtg 
      gagtcccgg aaaacaccg gaacctaaa acttccagt 
      tccggatct gagaaaaag aaaccggag gagaaaaag 
      gcgcggaaa agtgtgtcg gtgagcccg gatagaaaa 
      cacgtccat gtcaaagat gagaaaaaa gataacgac 
      cagaaacgg caccgcaag caccgaaag agccgccgg 
      gggaccctc accagcgtg gagcgcccc caccgcgag 
      cgctccttc tcggtgtgc accgaccgg tcggagcac 
      cgcttcggg gcccccttc gaggactcg gagcgcgaa 
      cgcaccaac agcctcagc agttgcgag acagtcaag 
      acgcgcaaa atgtccaac atttccaac attccgctc 
      aatggaaaa gtgccgtgg tgtgcgtgc tggggcaat 
      gggtgcatt tag
    mRNA sequence
    • >TC015026 RA
      acaaacgcg cgagagcga gaggcggcg tcccgacgt 
      gcgttgctt cgctcactt ttcgaaaca aattcgaat 
      cggatccga ctcaaagtc agtacgaca taaaaaaat 
      taaaacgtg aaaatgaaa tgctcaaaa aagaaatct 
      ggtgtttgc gtgacgaat acgacaaac tcgacaaac 
      tcctcaagg aaaaagaat acgaatttt cctcctcct 
      caaccactt gtagtacca gcgattctg aaagttgta 
      gcacatgca gtgaagaat cggaagaag ttgaaacgt 
      cgcaaaacc tcgaaaacg tccaaaaaa ccaacatga 
      aaaacctcc actcggaat tcgaccatt tatcggtcg 
      ttccggaag aaaacagta ctgaaaatt ccgacaaaa 
      gctcgcttc aagaaaata ttattaaac cagaccaga 
      cttacgaaa aatcaaaca ctttaccga acctccaac 
      cgaaaaatt ttttctcgc ccattacga taagtaaaa 
      gtgatgatg aattttaca ccgagaaag tggtgacca 
      ctaatgcga gcaattttt accagaaca tggttctag 
      tgccgaaaa gtgaaccat cggtggtac catcaatgg 
      gcaaaacca cgactgatt tattggtaa aaactgaca 
      atcacccct ttgatggta cccccagta ggagcactt 
      tcagcaatg gagggaatt tagaagaac tacttaatg 
      atattgaat caatttctc aggatattt taaaaatta 
      ccaacgacc aagctgccg aaaatagca ctcaaaaac 
      cgtacaaaa gtgaactaa acgttgtcc taatgccaa 
      acccgatgc cactgctcg gtttcgaaa aatacaaaa 
      acttacaaa acagtttcg aaagtttac actcgaaat 
      cggcggaaa aactcaaca attgtgatc aaacaaatc 
      catttttcg ccgaacaaa attcggatt ttttcaatt 
      cgcgttaca accctgaca attttcaaa aaggctcaa 
      aaactaatc ttctagaac tgaccaact cgtccgatt 
      tgatttcga gcgaaaatg aggaaaatc ttaaatttc 
      aaacgtcaa agaccgaaa acactgagg agaaaaaat 
      caccgaaag agagttttc gtaggaagg tttccatcc 
      attttaagg gtaaaaaag aaaagaaaa actcaattt 
      ttgatataa aatttggtg cttctgaac cgaaacatc 
      aaaaaacgc caagtgtgg agtcccgga aaacaccgg 
      aacctaaaa cttccagtt ccggatctg agaaaaaga 
      aaccggagg agaaaaagg cgcggaaaa gtgtgtcgg 
      tgagcccgg atagaaaac acgtccatg tcaaagatg 
      agaaaaaag ataacgacc agaaacggc accgcaagc 
      accgaaaga gccgccggg ggaccctca ccagcgtgg 
      agcgccccc accgcgagc gctccttct cggtgtgca 
      ccgaccggt cggagcacc gcttcgggg cccccttcg 
      aggactcgg agcgcgaac gcaccaaca gcctcagca 
      gttgcgaga cagtcaaga cgcgcaaaa tgtccaaca 
      tttccaaca ttccgctca atggaaaag tgccgtggt 
      gtgcgtgct ggggcaatg ggtgcattt agacactta 
      ccaagaatc gtcgatgtt gcgagcagg aagacacag 
      aggtgtttg ttcatgatt aggtcagcg acattgttt 
      ggcaccggg tctgggtca agggggcaa agggtcgat 
      gcacctgca ggagccaca aataaagca aggtgctaa 
      cggggcgct tttattact tacggttga taaacaaat 
      aaattgcac tggaaataa cgccgattt gtgtaaata 
      tacacaaag aatgttttg ggatggttt gacagatga 
      aaagttact aaaaataga aacgcacca aaaaaatgt 
      tggcaacgc cacgcgacg gaattttgc agtggacca 
      gtggactac tcacccaaa caaatcatt cgagaaaaa 
      atacatgtt cttttactt aaattaaat tgttgtgat 
      acca
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