| General information | |||
| TC015021 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5189088..5193652 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015021 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05742 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015021 RA
MFKKLKEKI TEEVKSSPQ RFQQLTQSV SDRLQNSST SDENFFSIA EDDATPSNS SFESGFTNV SLVSPQENK MRRNSNSSI ASDVSFLPR YESFANMYH LQSDLDISA SEIEDNASN SSQLGHLSK EQIYSAFQK AQMRYHKYR GRYTDLAKH YKDLERENA KMKSVLVET QDKAIRRVT ELKEQCALE QKAKAHLES ALRDEIDEK QFKIESLQT KIELLQTPE TENPDTLNK YLTDARVEI ENLNQRIQE MKANSIIFQ TKEQEYKAK ITNLEKEIA LFSEREKEN NLSLAQNKM ELHNELLSK DAEISNLKK SLEALTGGT GKFENLQTQ NTKLIEKLE ALTQKCNNY ENKLLKMEQ LRRENEENS RLKKEIQDI QGQLEGVRE EAKKSLLSL ETKIREKLA KEFEEKEER LRSDFRKKI EEVSSSSSN EFNLLEMEE EIRKLRQVN DGILEQLTS KTTKYDDLE RNHLELIEE SGRLRSQIG GLEKELATL QNTNQAANL KLGHLEANA RVLERTVEE YKRLEAERD DLLQEKKNF MEDLEEERR INERLNLEV EQLRDEMVK KSDLEHSLE EERKVTQKL NTELENVKC HIEEKKKDL ELTLDEEKG KLLQKLEES SVNMKHLED TGLALSEEL NSLKLKNEE LAKLAEERD TLLKKLTES EETNSLQTR KIQNLEEEM AKKAVSLEE AKRKILELG EQNNSLGHN LEEERNVNQ KLHSDLEHM KRHIEEKKK HLELSLDEE RAKLLQNLE ESSKTMKHL EETSLALTE ELNELKIRN EELAKVAEE RDTLLKKLT ESEENNSLQ ALQIQNLEE EMVKTVQTL EETKRKIQE LEEKNNSLQ HSLEEERNV NQKLTTELE HVKRHIEEK KKDLESTLD EERAKLLQK LEESSKTMK HLEETGFAL TEELNELKI NNAKVAEER DTLVKKLTD SEETNSLQT LKIQNLEEE MRNLFASLE NERKNSIKA GEELEEKEA NLKKLKMLL EDKENDFVR QLDEKESRL KKIEEMEEE ISSLKKLLD EANNNLAVK NHQIEIYEA DLKTKNGQI EALSRKIEE YNDLIEERN EGFFKILSN FSEPLNNVR RNKDNKHLY NLEQKLQNF EQTFSLERE NLAKILIYL KQFEEKHSA LEEEKEELL RQITELRKQ IVEVNSAQS YVKQFEEEI LRLREENVR LMEEQEKFK QNVLELESR NTQVVSDSN FLSEENHGL LTEIEELKL KNEEYETKH KNLLSQIEN LERESNQSQ LAELEKHLT ELQHEKQLL LEEIDELKT TPINAANKS EDYVKEIEN LRTKIVQYK SLDLTNRSS IEFYENELQ KLKNQNEKL NRKLDETLV TLNHCSELS NSTEIEYLK NVLYNYMLG KESMVLARV IAAVCKFDP QQTELILAK EQQKQTLLG QLGIL
- >TC015021 RA
atgttcaaa aaactcaag gagaagata actgaagag gtaaaaagc tctcctcaa agattccag caattgaca cagtctgta agtgaccgg ttacaaaat tcctcaact tcagacgaa aacttcttc tcgatagca gaagacgat gccacaccg tcaaactca agcttcgaa tcaggtttt accaatgtg tctttagtg tcacctcag gaaaataaa atgcggcgt aactccaac tcttcgatc gctagcgac gtctcgttt ttaccgcga tacgagtct ttcgccaac atgtatcat ctccagtcc gacttggac atctcagct agcgaaatt gaagacaac gcctcaaat tcctcacag ttgggacat ttgagcaag gagcagatt tactcagcc tttcagaaa gcgcaaatg cgctaccac aagtacagg gggcgctac acagacctg gccaaacac tataaagac ctagagcgg gaaaacgcc aaaatgaaa tcagtatta gtggaaaca caggacaag gcaataaga cgggtaact gaactcaag gaacagtgt gccttggaa cagaaggcc aaagcacac ttggaaagc gcactaagg gacgaaatc gacgaaaaa cagttcaaa atagagtca ctccaaacg aaaatcgaa cttctccaa acgcctgaa accgaaaat cccgacact ttaaacaag tacttaacg gacgcgaga gtcgaaatc gaaaatctg aaccaacga atccaggaa atgaaagcc aactcgatc atcttccaa accaaggag caagaatac aaagcgaaa atcacgaat ttggagaag gaaatcgcc cttttttcc gaacgcgag aaggagaat aatttaagc ctcgctcag aataaaatg gagttgcac aacgaactg ctcagtaaa gacgcagaa atcagcaat ttgaagaaa agtctcgaa gccctaact gggggcacc ggaaagttc gaaaatctt caaacccaa aatacgaaa ctcatcgaa aaacttgaa gccctaacc cagaagtgc aacaactac gaaaataaa ttgttaaaa atggaacaa ctccgacgc gaaaatgag gaaaattcg agactgaag aaagagata caagacatt caaggacaa ttggaggga gtgagagaa gaagcgaag aagagcctc ctaagtctc gaaacaaaa atccgggag aaacttgcc aaagaattt gaggaaaag gaagaaagg ctgaggagc gactttagg aagaaaatt gaagaagtg tcgtcaagt tcaagtaac gagttcaat cttcttgaa atggaggaa gaaattcgc aagttgagg caagttaat gacgggatt ttggaacaa ctgacctca aaaacaacg aaatatgac gatttggag cgaaatcat ctcgaattg attgaggaa agtgggcgg ttgaggagc caaatcggg ggtttggaa aaggagttg gcaacactg cagaataca aatcaggcg gcaaattta aaattgggc catttggag gcaaatgct agggttctg gaacgtacc gtggaggag tacaaacga ttggaggcc gaacgtgat gatttactc caagaaaag aagaatttc atggaggat ttggaggag gaaaggagg attaacgag agattgaat ttggaggtg gagcagttg agggacgaa atggtgaag aagagtgat cttgaacat agtttggag gaagaaagg aaagttacg cagaaatta aacacggaa ttggagaac gtcaaatgt cacattgaa gaaaagaag aaagatcta gaattgact ctggatgaa gaaaaaggc aagttgctt caaaaactt gaagaaagt tcggtgaat atgaagcat ttggaagat acgggtttg gcacttagt gaagaactc aactctttg aagctgaag aacgaagaa cttgcaaaa ctggctgaa gaaagggat acgttactg aagaaactt accgaaagt gaagaaacc aattcgtta caaacgcgg aaaatccag aatttggag gaagaaatg gcgaagaaa gccgtgtct ttggaagaa gcgaaaagg aagattcta gaattgggg gaacagaac aatagtctt ggacacaac ttggaggaa gaaagaaac gtaaaccag aaattacat tcggacttg gaacacatg aaacgtcac attgaagaa aagaagaaa catttggaa ttgtctttg gatgaagaa agggccaag ttgcttcaa aaccttgaa gaaagttcg aaaactatg aagcatttg gaagaaacg agtttggca cttactgaa gaactcaac gaattgaag ataagaaac gaagaactt gcaaaagtg gccgaagaa agggatacg ttattgaag aaacttacc gaaagtgaa gaaaacaat tcattacag gcgttgcaa attcagaat ttggaggaa gaaatggtg aagacagtg caaactttg gaagaaaca aaaaggaag attcaagaa ttggaggag aagaacaat agtcttcaa catagcttg gaggaagaa agaaacgta aaccagaaa ttaactacg gaattggag cacgtaaaa cgtcacatt gaagaaaag aagaaagat ttagaatcg actctggac gaagaaagg gcgaaattg cttcaaaaa cttgaagaa agttcgaag actatgaag catttggaa gaaacgggt tttgcactt actgaagaa ctcaacgaa ttgaagata aataatgct aaagtggcc gaagaaagg gatacctta gtgaagaaa ctcaccgac agtgaagaa accaattcg ttacaaacg ctcaaaata cagaatttg gaagaagaa atgaggaat ttgtttgcc agtttggaa aacgaacgg aagaatagt attaaggcg ggcgaagaa ctggaagaa aaggaagca aatttgaag aaactgaag atgctcctt gaagataag gaaaacgat tttgttaga caactggac gaaaaggag tcgcgtctt aagaaaatt gaagaaatg gaggaagaa atttcttca ttgaagaaa cttctagac gaagcgaat aataatttg gcggtaaaa aaccaccaa attgagatt tatgaagcc gatctgaag actaaaaac ggccagatt gaagcactg tcgaggaaa attgaagaa tataacgac ctaattgaa gaacggaac gaaggtttc ttcaaaatc ttgagcaac ttttccgag cctcttaat aatgttagg cgtaacaaa gacaataaa cacttgtat aatcttgaa cagaagctg caaaatttc gagcaaaca ttctccctt gagcgcgaa aatctcgcc aagatttta atttatttg aagcaattt gaagaaaaa cattcggct ttggaggaa gaaaaggaa gaattgttg aggcaaata accgaattg aggaaacaa atcgttgaa gtaaattcg gctcaaagt tatgtgaag caattcgag gaagaaatt cttcgattg cgggaagaa aatgtgcga ttgatggaa gaacaggag aagtttaag caaaatgtg ctcgaactc gagtcgaga aatacgcaa gttgtgtct gacagtaat tttctttcg gaagaaaat cacggtttg ctcaccgaa atcgaagaa ttgaaattg aagaatgaa gaatacgaa acgaagcat aaaaatttg ctatcgcag attgaaaat ctcgaacgt gagagtaac caaagccag ttggcagaa ctggaaaaa cacttaaca gagttacag cacgagaaa caactccta ttggaggaa atcgacgaa ttgaagacc acgcccatt aatgcggcc aacaaatca gaagattac gtcaaagaa attgagaat ttgcggacg aaaatcgtc caatacaaa tctctcgat ttgacgaat cgaagttcg attgaattt tacgaaaac gaattacaa aaattgaaa aaccagaac gagaaattg aaccgaaag ctggacgag actctagtc actttaaat cattgttcg gagctttcg aattcgacc gaaatcgag tatttgaag aacgttttg tataattat atgctaggg aaggagagt atggtgttg gcaagggtt attgcagct gtgtgtaag tttgatccg caacaaacc gaattaatt ctagcgaaa gagcagcaa aagcaaact ttgttgggc caattgggg attctctaa
- >TC015021 RA
taaggttat aggagtgtt tgtttattt tgttaaaat tctcgcttg ccccgtttc acaatgttc aaaaaactc aaggagaag ataactgaa gaggtaaaa agctctcct caaagattc cagcaattg acacagtct gtaagtgac cggttacaa aattcctca acttcagac gaaaacttc ttctcgata gcagaagac gatgccaca ccgtcaaac tcaagcttc gaatcaggt tttaccaat gtgtcttta gtgtcacct caggaaaat aaaatgcgg cgtaactcc aactcttcg atcgctagc gacgtctcg tttttaccg cgatacgag tctttcgcc aacatgtat catctccag tccgacttg gacatctca gctagcgaa attgaagac aacgcctca aattcctca cagttggga catttgagc aaggagcag atttactca gcctttcag aaagcgcaa atgcgctac cacaagtac agggggcgc tacacagac ctggccaaa cactataaa gacctagag cgggaaaac gccaaaatg aaatcagta ttagtggaa acacaggac aaggcaata agacgggta actgaactc aaggaacag tgtgccttg gaacagaag gccaaagca cacttggaa agcgcacta agggacgaa atcgacgaa aaacagttc aaaatagag tcactccaa acgaaaatc gaacttctc caaacgcct gaaaccgaa aatcccgac actttaaac aagtactta acggacgcg agagtcgaa atcgaaaat ctgaaccaa cgaatccag gaaatgaaa gccaactcg atcatcttc caaaccaag gagcaagaa tacaaagcg aaaatcacg aatttggag aaggaaatc gcccttttt tccgaacgc gagaaggag aataattta agcctcgct cagaataaa atggagttg cacaacgaa ctgctcagt aaagacgca gaaatcagc aatttgaag aaaagtctc gaagcccta actgggggc accggaaag ttcgaaaat cttcaaacc caaaatacg aaactcatc gaaaaactt gaagcccta acccagaag tgcaacaac tacgaaaat aaattgtta aaaatggaa caactccga cgcgaaaat gaggaaaat tcgagactg aagaaagag atacaagac attcaagga caattggag ggagtgaga gaagaagcg aagaagagc ctcctaagt ctcgaaaca aaaatccgg gagaaactt gccaaagaa tttgaggaa aaggaagaa aggctgagg agcgacttt aggaagaaa attgaagaa gtgtcgtca agttcaagt aacgagttc aatcttctt gaaatggag gaagaaatt cgcaagttg aggcaagtt aatgacggg attttggaa caactgacc tcaaaaaca acgaaatat gacgatttg gagcgaaat catctcgaa ttgattgag gaaagtggg cggttgagg agccaaatc gggggtttg gaaaaggag ttggcaaca ctgcagaat acaaatcag gcggcaaat ttaaaattg ggccatttg gaggcaaat gctagggtt ctggaacgt accgtggag gagtacaaa cgattggag gccgaacgt gatgattta ctccaagaa aagaagaat ttcatggag gatttggag gaggaaagg aggattaac gagagattg aatttggag gtggagcag ttgagggac gaaatggtg aagaagagt gatcttgaa catagtttg gaggaagaa aggaaagtt acgcagaaa ttaaacacg gaattggag aacgtcaaa tgtcacatt gaagaaaag aagaaagat ctagaattg actctggat gaagaaaaa ggcaagttg cttcaaaaa cttgaagaa agttcggtg aatatgaag catttggaa gatacgggt ttggcactt agtgaagaa ctcaactct ttgaagctg aagaacgaa gaacttgca aaactggct gaagaaagg gatacgtta ctgaagaaa cttaccgaa agtgaagaa accaattcg ttacaaacg cggaaaatc cagaatttg gaggaagaa atggcgaag aaagccgtg tctttggaa gaagcgaaa aggaagatt ctagaattg ggggaacag aacaatagt cttggacac aacttggag gaagaaaga aacgtaaac cagaaatta cattcggac ttggaacac atgaaacgt cacattgaa gaaaagaag aaacatttg gaattgtct ttggatgaa gaaagggcc aagttgctt caaaacctt gaagaaagt tcgaaaact atgaagcat ttggaagaa acgagtttg gcacttact gaagaactc aacgaattg aagataaga aacgaagaa cttgcaaaa gtggccgaa gaaagggat acgttattg aagaaactt accgaaagt gaagaaaac aattcatta caggcgttg caaattcag aatttggag gaagaaatg gtgaagaca gtgcaaact ttggaagaa acaaaaagg aagattcaa gaattggag gagaagaac aatagtctt caacatagc ttggaggaa gaaagaaac gtaaaccag aaattaact acggaattg gagcacgta aaacgtcac attgaagaa aagaagaaa gatttagaa tcgactctg gacgaagaa agggcgaaa ttgcttcaa aaacttgaa gaaagttcg aagactatg aagcatttg gaagaaacg ggttttgca cttactgaa gaactcaac gaattgaag ataaataat gctaaagtg gccgaagaa agggatacc ttagtgaag aaactcacc gacagtgaa gaaaccaat tcgttacaa acgctcaaa atacagaat ttggaagaa gaaatgagg aatttgttt gccagtttg gaaaacgaa cggaagaat agtattaag gcgggcgaa gaactggaa gaaaaggaa gcaaatttg aagaaactg aagatgctc cttgaagat aaggaaaac gattttgtt agacaactg gacgaaaag gagtcgcgt cttaagaaa attgaagaa atggaggaa gaaatttct tcattgaag aaacttcta gacgaagcg aataataat ttggcggta aaaaaccac caaattgag atttatgaa gccgatctg aagactaaa aacggccag attgaagca ctgtcgagg aaaattgaa gaatataac gacctaatt gaagaacgg aacgaaggt ttcttcaaa atcttgagc aacttttcc gagcctctt aataatgtt aggcgtaac aaagacaat aaacacttg tataatctt gaacagaag ctgcaaaat ttcgagcaa acattctcc cttgagcgc gaaaatctc gccaagatt ttaatttat ttgaagcaa tttgaagaa aaacattcg gctttggag gaagaaaag gaagaattg ttgaggcaa ataaccgaa ttgaggaaa caaatcgtt gaagtaaat tcggctcaa agttatgtg aagcaattc gaggaagaa attcttcga ttgcgggaa gaaaatgtg cgattgatg gaagaacag gagaagttt aagcaaaat gtgctcgaa ctcgagtcg agaaatacg caagttgtg tctgacagt aattttctt tcggaagaa aatcacggt ttgctcacc gaaatcgaa gaattgaaa ttgaagaat gaagaatac gaaacgaag cataaaaat ttgctatcg cagattgaa aatctcgaa cgtgagagt aaccaaagc cagttggca gaactggaa aaacactta acagagtta cagcacgag aaacaactc ctattggag gaaatcgac gaattgaag accacgccc attaatgcg gccaacaaa tcagaagat tacgtcaaa gaaattgag aatttgcgg acgaaaatc gtccaatac aaatctctc gatttgacg aatcgaagt tcgattgaa ttttacgaa aacgaatta caaaaattg aaaaaccag aacgagaaa ttgaaccga aagctggac gagactcta gtcacttta aatcattgt tcggagctt tcgaattcg accgaaatc gagtatttg aagaacgtt ttgtataat tatatgcta gggaaggag agtatggtg ttggcaagg gttattgca gctgtgtgt aagtttgat ccgcaacaa accgaatta attctagcg aaagagcag caaaagcaa actttgttg ggccaattg gggattctc taacagtag gtccacgtt ttaacttag gtgcattcc gtttactca ttacgtacg atttattaa gcgattttg tcagtacta acgcacttt ttatcggtt gtgtacata tttcgttgt atcttataa tatttatgt aaattgtta aataaacta aactttcgt aataaattg tgataactt ac
