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Gene information
General information
TC015021 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5189088..5193652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015021
    iBeetle screen iB_05742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015021 RA
      MFKKLKEKI TEEVKSSPQ RFQQLTQSV SDRLQNSST 
      SDENFFSIA EDDATPSNS SFESGFTNV SLVSPQENK 
      MRRNSNSSI ASDVSFLPR YESFANMYH LQSDLDISA 
      SEIEDNASN SSQLGHLSK EQIYSAFQK AQMRYHKYR 
      GRYTDLAKH YKDLERENA KMKSVLVET QDKAIRRVT 
      ELKEQCALE QKAKAHLES ALRDEIDEK QFKIESLQT 
      KIELLQTPE TENPDTLNK YLTDARVEI ENLNQRIQE 
      MKANSIIFQ TKEQEYKAK ITNLEKEIA LFSEREKEN 
      NLSLAQNKM ELHNELLSK DAEISNLKK SLEALTGGT 
      GKFENLQTQ NTKLIEKLE ALTQKCNNY ENKLLKMEQ 
      LRRENEENS RLKKEIQDI QGQLEGVRE EAKKSLLSL 
      ETKIREKLA KEFEEKEER LRSDFRKKI EEVSSSSSN 
      EFNLLEMEE EIRKLRQVN DGILEQLTS KTTKYDDLE 
      RNHLELIEE SGRLRSQIG GLEKELATL QNTNQAANL 
      KLGHLEANA RVLERTVEE YKRLEAERD DLLQEKKNF 
      MEDLEEERR INERLNLEV EQLRDEMVK KSDLEHSLE 
      EERKVTQKL NTELENVKC HIEEKKKDL ELTLDEEKG 
      KLLQKLEES SVNMKHLED TGLALSEEL NSLKLKNEE 
      LAKLAEERD TLLKKLTES EETNSLQTR KIQNLEEEM 
      AKKAVSLEE AKRKILELG EQNNSLGHN LEEERNVNQ 
      KLHSDLEHM KRHIEEKKK HLELSLDEE RAKLLQNLE 
      ESSKTMKHL EETSLALTE ELNELKIRN EELAKVAEE 
      RDTLLKKLT ESEENNSLQ ALQIQNLEE EMVKTVQTL 
      EETKRKIQE LEEKNNSLQ HSLEEERNV NQKLTTELE 
      HVKRHIEEK KKDLESTLD EERAKLLQK LEESSKTMK 
      HLEETGFAL TEELNELKI NNAKVAEER DTLVKKLTD 
      SEETNSLQT LKIQNLEEE MRNLFASLE NERKNSIKA 
      GEELEEKEA NLKKLKMLL EDKENDFVR QLDEKESRL 
      KKIEEMEEE ISSLKKLLD EANNNLAVK NHQIEIYEA 
      DLKTKNGQI EALSRKIEE YNDLIEERN EGFFKILSN 
      FSEPLNNVR RNKDNKHLY NLEQKLQNF EQTFSLERE 
      NLAKILIYL KQFEEKHSA LEEEKEELL RQITELRKQ 
      IVEVNSAQS YVKQFEEEI LRLREENVR LMEEQEKFK 
      QNVLELESR NTQVVSDSN FLSEENHGL LTEIEELKL 
      KNEEYETKH KNLLSQIEN LERESNQSQ LAELEKHLT 
      ELQHEKQLL LEEIDELKT TPINAANKS EDYVKEIEN 
      LRTKIVQYK SLDLTNRSS IEFYENELQ KLKNQNEKL 
      NRKLDETLV TLNHCSELS NSTEIEYLK NVLYNYMLG 
      KESMVLARV IAAVCKFDP QQTELILAK EQQKQTLLG 
      QLGIL
    CDS sequence
    • >TC015021 RA
      atgttcaaa aaactcaag gagaagata actgaagag 
      gtaaaaagc tctcctcaa agattccag caattgaca 
      cagtctgta agtgaccgg ttacaaaat tcctcaact 
      tcagacgaa aacttcttc tcgatagca gaagacgat 
      gccacaccg tcaaactca agcttcgaa tcaggtttt 
      accaatgtg tctttagtg tcacctcag gaaaataaa 
      atgcggcgt aactccaac tcttcgatc gctagcgac 
      gtctcgttt ttaccgcga tacgagtct ttcgccaac 
      atgtatcat ctccagtcc gacttggac atctcagct 
      agcgaaatt gaagacaac gcctcaaat tcctcacag 
      ttgggacat ttgagcaag gagcagatt tactcagcc 
      tttcagaaa gcgcaaatg cgctaccac aagtacagg 
      gggcgctac acagacctg gccaaacac tataaagac 
      ctagagcgg gaaaacgcc aaaatgaaa tcagtatta 
      gtggaaaca caggacaag gcaataaga cgggtaact 
      gaactcaag gaacagtgt gccttggaa cagaaggcc 
      aaagcacac ttggaaagc gcactaagg gacgaaatc 
      gacgaaaaa cagttcaaa atagagtca ctccaaacg 
      aaaatcgaa cttctccaa acgcctgaa accgaaaat 
      cccgacact ttaaacaag tacttaacg gacgcgaga 
      gtcgaaatc gaaaatctg aaccaacga atccaggaa 
      atgaaagcc aactcgatc atcttccaa accaaggag 
      caagaatac aaagcgaaa atcacgaat ttggagaag 
      gaaatcgcc cttttttcc gaacgcgag aaggagaat 
      aatttaagc ctcgctcag aataaaatg gagttgcac 
      aacgaactg ctcagtaaa gacgcagaa atcagcaat 
      ttgaagaaa agtctcgaa gccctaact gggggcacc 
      ggaaagttc gaaaatctt caaacccaa aatacgaaa 
      ctcatcgaa aaacttgaa gccctaacc cagaagtgc 
      aacaactac gaaaataaa ttgttaaaa atggaacaa 
      ctccgacgc gaaaatgag gaaaattcg agactgaag 
      aaagagata caagacatt caaggacaa ttggaggga 
      gtgagagaa gaagcgaag aagagcctc ctaagtctc 
      gaaacaaaa atccgggag aaacttgcc aaagaattt 
      gaggaaaag gaagaaagg ctgaggagc gactttagg 
      aagaaaatt gaagaagtg tcgtcaagt tcaagtaac 
      gagttcaat cttcttgaa atggaggaa gaaattcgc 
      aagttgagg caagttaat gacgggatt ttggaacaa 
      ctgacctca aaaacaacg aaatatgac gatttggag 
      cgaaatcat ctcgaattg attgaggaa agtgggcgg 
      ttgaggagc caaatcggg ggtttggaa aaggagttg 
      gcaacactg cagaataca aatcaggcg gcaaattta 
      aaattgggc catttggag gcaaatgct agggttctg 
      gaacgtacc gtggaggag tacaaacga ttggaggcc 
      gaacgtgat gatttactc caagaaaag aagaatttc 
      atggaggat ttggaggag gaaaggagg attaacgag 
      agattgaat ttggaggtg gagcagttg agggacgaa 
      atggtgaag aagagtgat cttgaacat agtttggag 
      gaagaaagg aaagttacg cagaaatta aacacggaa 
      ttggagaac gtcaaatgt cacattgaa gaaaagaag 
      aaagatcta gaattgact ctggatgaa gaaaaaggc 
      aagttgctt caaaaactt gaagaaagt tcggtgaat 
      atgaagcat ttggaagat acgggtttg gcacttagt 
      gaagaactc aactctttg aagctgaag aacgaagaa 
      cttgcaaaa ctggctgaa gaaagggat acgttactg 
      aagaaactt accgaaagt gaagaaacc aattcgtta 
      caaacgcgg aaaatccag aatttggag gaagaaatg 
      gcgaagaaa gccgtgtct ttggaagaa gcgaaaagg 
      aagattcta gaattgggg gaacagaac aatagtctt 
      ggacacaac ttggaggaa gaaagaaac gtaaaccag 
      aaattacat tcggacttg gaacacatg aaacgtcac 
      attgaagaa aagaagaaa catttggaa ttgtctttg 
      gatgaagaa agggccaag ttgcttcaa aaccttgaa 
      gaaagttcg aaaactatg aagcatttg gaagaaacg 
      agtttggca cttactgaa gaactcaac gaattgaag 
      ataagaaac gaagaactt gcaaaagtg gccgaagaa 
      agggatacg ttattgaag aaacttacc gaaagtgaa 
      gaaaacaat tcattacag gcgttgcaa attcagaat 
      ttggaggaa gaaatggtg aagacagtg caaactttg 
      gaagaaaca aaaaggaag attcaagaa ttggaggag 
      aagaacaat agtcttcaa catagcttg gaggaagaa 
      agaaacgta aaccagaaa ttaactacg gaattggag 
      cacgtaaaa cgtcacatt gaagaaaag aagaaagat 
      ttagaatcg actctggac gaagaaagg gcgaaattg 
      cttcaaaaa cttgaagaa agttcgaag actatgaag 
      catttggaa gaaacgggt tttgcactt actgaagaa 
      ctcaacgaa ttgaagata aataatgct aaagtggcc 
      gaagaaagg gatacctta gtgaagaaa ctcaccgac 
      agtgaagaa accaattcg ttacaaacg ctcaaaata 
      cagaatttg gaagaagaa atgaggaat ttgtttgcc 
      agtttggaa aacgaacgg aagaatagt attaaggcg 
      ggcgaagaa ctggaagaa aaggaagca aatttgaag 
      aaactgaag atgctcctt gaagataag gaaaacgat 
      tttgttaga caactggac gaaaaggag tcgcgtctt 
      aagaaaatt gaagaaatg gaggaagaa atttcttca 
      ttgaagaaa cttctagac gaagcgaat aataatttg 
      gcggtaaaa aaccaccaa attgagatt tatgaagcc 
      gatctgaag actaaaaac ggccagatt gaagcactg 
      tcgaggaaa attgaagaa tataacgac ctaattgaa 
      gaacggaac gaaggtttc ttcaaaatc ttgagcaac 
      ttttccgag cctcttaat aatgttagg cgtaacaaa 
      gacaataaa cacttgtat aatcttgaa cagaagctg 
      caaaatttc gagcaaaca ttctccctt gagcgcgaa 
      aatctcgcc aagatttta atttatttg aagcaattt 
      gaagaaaaa cattcggct ttggaggaa gaaaaggaa 
      gaattgttg aggcaaata accgaattg aggaaacaa 
      atcgttgaa gtaaattcg gctcaaagt tatgtgaag 
      caattcgag gaagaaatt cttcgattg cgggaagaa 
      aatgtgcga ttgatggaa gaacaggag aagtttaag 
      caaaatgtg ctcgaactc gagtcgaga aatacgcaa 
      gttgtgtct gacagtaat tttctttcg gaagaaaat 
      cacggtttg ctcaccgaa atcgaagaa ttgaaattg 
      aagaatgaa gaatacgaa acgaagcat aaaaatttg 
      ctatcgcag attgaaaat ctcgaacgt gagagtaac 
      caaagccag ttggcagaa ctggaaaaa cacttaaca 
      gagttacag cacgagaaa caactccta ttggaggaa 
      atcgacgaa ttgaagacc acgcccatt aatgcggcc 
      aacaaatca gaagattac gtcaaagaa attgagaat 
      ttgcggacg aaaatcgtc caatacaaa tctctcgat 
      ttgacgaat cgaagttcg attgaattt tacgaaaac 
      gaattacaa aaattgaaa aaccagaac gagaaattg 
      aaccgaaag ctggacgag actctagtc actttaaat 
      cattgttcg gagctttcg aattcgacc gaaatcgag 
      tatttgaag aacgttttg tataattat atgctaggg 
      aaggagagt atggtgttg gcaagggtt attgcagct 
      gtgtgtaag tttgatccg caacaaacc gaattaatt 
      ctagcgaaa gagcagcaa aagcaaact ttgttgggc 
      caattgggg attctctaa
    mRNA sequence
    • >TC015021 RA
      taaggttat aggagtgtt tgtttattt tgttaaaat 
      tctcgcttg ccccgtttc acaatgttc aaaaaactc 
      aaggagaag ataactgaa gaggtaaaa agctctcct 
      caaagattc cagcaattg acacagtct gtaagtgac 
      cggttacaa aattcctca acttcagac gaaaacttc 
      ttctcgata gcagaagac gatgccaca ccgtcaaac 
      tcaagcttc gaatcaggt tttaccaat gtgtcttta 
      gtgtcacct caggaaaat aaaatgcgg cgtaactcc 
      aactcttcg atcgctagc gacgtctcg tttttaccg 
      cgatacgag tctttcgcc aacatgtat catctccag 
      tccgacttg gacatctca gctagcgaa attgaagac 
      aacgcctca aattcctca cagttggga catttgagc 
      aaggagcag atttactca gcctttcag aaagcgcaa 
      atgcgctac cacaagtac agggggcgc tacacagac 
      ctggccaaa cactataaa gacctagag cgggaaaac 
      gccaaaatg aaatcagta ttagtggaa acacaggac 
      aaggcaata agacgggta actgaactc aaggaacag 
      tgtgccttg gaacagaag gccaaagca cacttggaa 
      agcgcacta agggacgaa atcgacgaa aaacagttc 
      aaaatagag tcactccaa acgaaaatc gaacttctc 
      caaacgcct gaaaccgaa aatcccgac actttaaac 
      aagtactta acggacgcg agagtcgaa atcgaaaat 
      ctgaaccaa cgaatccag gaaatgaaa gccaactcg 
      atcatcttc caaaccaag gagcaagaa tacaaagcg 
      aaaatcacg aatttggag aaggaaatc gcccttttt 
      tccgaacgc gagaaggag aataattta agcctcgct 
      cagaataaa atggagttg cacaacgaa ctgctcagt 
      aaagacgca gaaatcagc aatttgaag aaaagtctc 
      gaagcccta actgggggc accggaaag ttcgaaaat 
      cttcaaacc caaaatacg aaactcatc gaaaaactt 
      gaagcccta acccagaag tgcaacaac tacgaaaat 
      aaattgtta aaaatggaa caactccga cgcgaaaat 
      gaggaaaat tcgagactg aagaaagag atacaagac 
      attcaagga caattggag ggagtgaga gaagaagcg 
      aagaagagc ctcctaagt ctcgaaaca aaaatccgg 
      gagaaactt gccaaagaa tttgaggaa aaggaagaa 
      aggctgagg agcgacttt aggaagaaa attgaagaa 
      gtgtcgtca agttcaagt aacgagttc aatcttctt 
      gaaatggag gaagaaatt cgcaagttg aggcaagtt 
      aatgacggg attttggaa caactgacc tcaaaaaca 
      acgaaatat gacgatttg gagcgaaat catctcgaa 
      ttgattgag gaaagtggg cggttgagg agccaaatc 
      gggggtttg gaaaaggag ttggcaaca ctgcagaat 
      acaaatcag gcggcaaat ttaaaattg ggccatttg 
      gaggcaaat gctagggtt ctggaacgt accgtggag 
      gagtacaaa cgattggag gccgaacgt gatgattta 
      ctccaagaa aagaagaat ttcatggag gatttggag 
      gaggaaagg aggattaac gagagattg aatttggag 
      gtggagcag ttgagggac gaaatggtg aagaagagt 
      gatcttgaa catagtttg gaggaagaa aggaaagtt 
      acgcagaaa ttaaacacg gaattggag aacgtcaaa 
      tgtcacatt gaagaaaag aagaaagat ctagaattg 
      actctggat gaagaaaaa ggcaagttg cttcaaaaa 
      cttgaagaa agttcggtg aatatgaag catttggaa 
      gatacgggt ttggcactt agtgaagaa ctcaactct 
      ttgaagctg aagaacgaa gaacttgca aaactggct 
      gaagaaagg gatacgtta ctgaagaaa cttaccgaa 
      agtgaagaa accaattcg ttacaaacg cggaaaatc 
      cagaatttg gaggaagaa atggcgaag aaagccgtg 
      tctttggaa gaagcgaaa aggaagatt ctagaattg 
      ggggaacag aacaatagt cttggacac aacttggag 
      gaagaaaga aacgtaaac cagaaatta cattcggac 
      ttggaacac atgaaacgt cacattgaa gaaaagaag 
      aaacatttg gaattgtct ttggatgaa gaaagggcc 
      aagttgctt caaaacctt gaagaaagt tcgaaaact 
      atgaagcat ttggaagaa acgagtttg gcacttact 
      gaagaactc aacgaattg aagataaga aacgaagaa 
      cttgcaaaa gtggccgaa gaaagggat acgttattg 
      aagaaactt accgaaagt gaagaaaac aattcatta 
      caggcgttg caaattcag aatttggag gaagaaatg 
      gtgaagaca gtgcaaact ttggaagaa acaaaaagg 
      aagattcaa gaattggag gagaagaac aatagtctt 
      caacatagc ttggaggaa gaaagaaac gtaaaccag 
      aaattaact acggaattg gagcacgta aaacgtcac 
      attgaagaa aagaagaaa gatttagaa tcgactctg 
      gacgaagaa agggcgaaa ttgcttcaa aaacttgaa 
      gaaagttcg aagactatg aagcatttg gaagaaacg 
      ggttttgca cttactgaa gaactcaac gaattgaag 
      ataaataat gctaaagtg gccgaagaa agggatacc 
      ttagtgaag aaactcacc gacagtgaa gaaaccaat 
      tcgttacaa acgctcaaa atacagaat ttggaagaa 
      gaaatgagg aatttgttt gccagtttg gaaaacgaa 
      cggaagaat agtattaag gcgggcgaa gaactggaa 
      gaaaaggaa gcaaatttg aagaaactg aagatgctc 
      cttgaagat aaggaaaac gattttgtt agacaactg 
      gacgaaaag gagtcgcgt cttaagaaa attgaagaa 
      atggaggaa gaaatttct tcattgaag aaacttcta 
      gacgaagcg aataataat ttggcggta aaaaaccac 
      caaattgag atttatgaa gccgatctg aagactaaa 
      aacggccag attgaagca ctgtcgagg aaaattgaa 
      gaatataac gacctaatt gaagaacgg aacgaaggt 
      ttcttcaaa atcttgagc aacttttcc gagcctctt 
      aataatgtt aggcgtaac aaagacaat aaacacttg 
      tataatctt gaacagaag ctgcaaaat ttcgagcaa 
      acattctcc cttgagcgc gaaaatctc gccaagatt 
      ttaatttat ttgaagcaa tttgaagaa aaacattcg 
      gctttggag gaagaaaag gaagaattg ttgaggcaa 
      ataaccgaa ttgaggaaa caaatcgtt gaagtaaat 
      tcggctcaa agttatgtg aagcaattc gaggaagaa 
      attcttcga ttgcgggaa gaaaatgtg cgattgatg 
      gaagaacag gagaagttt aagcaaaat gtgctcgaa 
      ctcgagtcg agaaatacg caagttgtg tctgacagt 
      aattttctt tcggaagaa aatcacggt ttgctcacc 
      gaaatcgaa gaattgaaa ttgaagaat gaagaatac 
      gaaacgaag cataaaaat ttgctatcg cagattgaa 
      aatctcgaa cgtgagagt aaccaaagc cagttggca 
      gaactggaa aaacactta acagagtta cagcacgag 
      aaacaactc ctattggag gaaatcgac gaattgaag 
      accacgccc attaatgcg gccaacaaa tcagaagat 
      tacgtcaaa gaaattgag aatttgcgg acgaaaatc 
      gtccaatac aaatctctc gatttgacg aatcgaagt 
      tcgattgaa ttttacgaa aacgaatta caaaaattg 
      aaaaaccag aacgagaaa ttgaaccga aagctggac 
      gagactcta gtcacttta aatcattgt tcggagctt 
      tcgaattcg accgaaatc gagtatttg aagaacgtt 
      ttgtataat tatatgcta gggaaggag agtatggtg 
      ttggcaagg gttattgca gctgtgtgt aagtttgat 
      ccgcaacaa accgaatta attctagcg aaagagcag 
      caaaagcaa actttgttg ggccaattg gggattctc 
      taacagtag gtccacgtt ttaacttag gtgcattcc 
      gtttactca ttacgtacg atttattaa gcgattttg 
      tcagtacta acgcacttt ttatcggtt gtgtacata 
      tttcgttgt atcttataa tatttatgt aaattgtta 
      aataaacta aactttcgt aataaattg tgataactt 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY