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Gene information
General information
TC015006 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4987769..4989668
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015006
    iBeetle screen iB_05740

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015006 RA
      MRKLSDEDK YQQQQKQLE RELVSLRRE FATLERDSS 
      QFNSLKKLK KYTKVFNIF KNEYENIVT NLEVAAAPG 
      YERRDKIMY EELSQRLHD YNCYHDGEI LKKTQMKEL 
      EGQIRNVSK AVQCLRNQQ ITDRQFEER VHNARQTLE 
      TLENKLETQ MTTFNLIVA KNGKLREEI DHLLWDRRN 
      FLTIWNRFI RKLNTGKRI LIDLIEQAT IAYDQREEW 
      CNKLQLLRS SAHRDLLAN IDQMRSLKQ KVDNAKKLE 
      EFFTTKGQK RVMKDLMKK EREKREERK KKLGTKLQH 
      YESLMNEIL DFSQHAEIK DIARKYYNR EAQNFSAFK 
      FIADTINNM EMINDQLGI LHLEIDELK AVHDLRAET 
      QHETIDNLE TDLVQASEE TKNAQQDLE DLNLHLKSV 
      MQGVTELFR MCKCDKDPL LKLLGDNAT IHEYNVLLF 
      LQLLEKTIQ IYLITAGYK DKVQAEKRS SGKAKILAT 
      VDTTTFIYP IERIVRADP CSLCIEHEM VSDVIDVVQ 
      RPWTRKEAK EMLQQRLDL PGASTKLHT VSKCFLPQA 
      RHIKQKKYC
    CDS sequence
    • >TC015006 RA
      atgcgcaaa ctttcagac gaagataag taccaacaa 
      cagcaaaaa cagctggaa cgcgagttg gtttcgcta 
      cgtcgagag tttgccacc ttagaacgc gacagttcg 
      caattcaac agcttgaaa aaactaaaa aagtacacg 
      aaagtgttc aacattttt aagaatgag tacgagaac 
      attgtcacg aatttggaa gtggccgca gcacctggt 
      tacgaacgc agggacaaa atcatgtac gaggaatta 
      tcccagcgt ttgcacgac tacaactgc tatcacgac 
      ggtgaaatc ctcaaaaaa actcaaatg aaggagctg 
      gaaggccag attcggaac gtgtcaaaa gcggtccag 
      tgtttgcgg aaccaacaa atcactgat cggcaattc 
      gaagaacga gtccacaac gctcgccaa actttggaa 
      actttggag aacaaactt gaaacacaa atgaccacg 
      tttaaccta atagtggcc aaaaatggg aaattgagg 
      gaagagatc gaccatctc ctgtgggac agacgcaac 
      tttttgacc atctggaac cgatttatc agaaagttg 
      aacactggt aagagaatt ttgatcgac ttgatcgag 
      caagcgacc attgcctac gaccagagg gaggaatgg 
      tgcaacaaa ctgcagttg ttgcgaagt agtgcccat 
      cgggacctt ttggcaaac attgaccag atgagaagt 
      ttgaagcaa aaagtggac aatgccaaa aaactcgaa 
      gagtttttc accacaaaa ggccaaaag cgagtcatg 
      aaagacctg atgaagaag gagagagaa aaacgggaa 
      gagcgcaaa aagaagctt ggaacaaaa ttgcaacat 
      tatgagagt ctaatgaac gaaatattg gatttttcc 
      caacatgct gaaatcaag gacattgcc cggaagtac 
      tacaaccgg gaggcccag aactttagc gcatttaaa 
      ttcatcgca gacacgatc aacaacatg gaaatgatc 
      aacgaccag cttggtatt ttgcacttg gaaatcgat 
      gagttgaaa gctgtgcac gatctgagg gccgaaacc 
      cagcacgag acaatcgat aatttggaa acagacctg 
      gttcaggcg agtgaagag acgaaaaac gcacaacaa 
      gacttggaa gatttaaac ttgcattta aaatccgtg 
      atgcaagga gtcaccgaa ctgttcaga atgtgcaaa 
      tgtgacaaa gatccgctt ttgaagctt ttgggtgac 
      aatgccacc attcacgaa tacaacgtt ttgcttttt 
      ctacaactt ctggagaag acgattcag atctatctc 
      atcacagct ggttacaaa gataaagtt caggctgag 
      aaacggtcg tcagggaag gcaaaaatt ttggctacg 
      gttgacacc accacgttt atttaccca attgagagg 
      attgtgaga gctgatcct tgctctttg tgtattgag 
      catgagatg gtttctgat gttatcgat gttgtacaa 
      cgtccttgg actaggaaa gaagccaaa gaaatgctt 
      caacaacgg ttggatttg ccaggtgct tcaaccaaa 
      ttgcacacg gtttccaag tgcttttta ccgcaagct 
      cgacacatc aagcaaaag aaatactgt tag
    mRNA sequence
    • >TC015006 RA
      tttttgtta atcacgttt ttttttgta ccatgtctt 
      ccggctaca aaatgcgca aactttcag acgaagata 
      agtaccaac aacagcaaa aacagctgg aacgcgagt 
      tggtttcgc tacgtcgag agtttgcca ccttagaac 
      gcgacagtt cgcaattca acagcttga aaaaactaa 
      aaaagtaca cgaaagtgt tcaacattt ttaagaatg 
      agtacgaga acattgtca cgaatttgg aagtggccg 
      cagcacctg gttacgaac gcagggaca aaatcatgt 
      acgaggaat tatcccagc gtttgcacg actacaact 
      gctatcacg acggtgaaa tcctcaaaa aaactcaaa 
      tgaaggagc tggaaggcc agattcgga acgtgtcaa 
      aagcggtcc agtgtttgc ggaaccaac aaatcactg 
      atcggcaat tcgaagaac gagtccaca acgctcgcc 
      aaactttgg aaactttgg agaacaaac ttgaaacac 
      aaatgacca cgtttaacc taatagtgg ccaaaaatg 
      ggaaattga gggaagaga tcgaccatc tcctgtggg 
      acagacgca actttttga ccatctgga accgattta 
      tcagaaagt tgaacactg gtaagagaa ttttgatcg 
      acttgatcg agcaagcga ccattgcct acgaccaga 
      gggaggaat ggtgcaaca aactgcagt tgttgcgaa 
      gtagtgccc atcgggacc ttttggcaa acattgacc 
      agatgagaa gtttgaagc aaaaagtgg acaatgcca 
      aaaaactcg aagagtttt tcaccacaa aaggccaaa 
      agcgagtca tgaaagacc tgatgaaga aggagagag 
      aaaaacggg aagagcgca aaaagaagc ttggaacaa 
      aattgcaac attatgaga gtctaatga acgaaatat 
      tggattttt cccaacatg ctgaaatca aggacattg 
      cccggaagt actacaacc gggaggccc agaacttta 
      gcgcattta aattcatcg cagacacga tcaacaaca 
      tggaaatga tcaacgacc agcttggta ttttgcact 
      tggaaatcg atgagttga aagctgtgc acgatctga 
      gggccgaaa cccagcacg agacaatcg ataatttgg 
      aaacagacc tggttcagg cgagtgaag agacgaaaa 
      acgcacaac aagacttgg aagatttaa acttgcatt 
      taaaatccg tgatgcaag gagtcaccg aactgttca 
      gaatgtgca aatgtgaca aagatccgc ttttgaagc 
      ttttgggtg acaatgcca ccattcacg aatacaacg 
      ttttgcttt ttctacaac ttctggaga agacgattc 
      agatctatc tcatcacag ctggttaca aagataaag 
      ttcaggctg agaaacggt cgtcaggga aggcaaaaa 
      ttttggcta cggttgaca ccaccacgt ttatttacc 
      caattgaga ggattgtga gagctgatc cttgctctt 
      tgtgtattg agcatgaga tggtttctg atgttatcg 
      atgttgtac aacgtcctt ggactagga aagaagcca 
      aagaaatgc ttcaacaac ggttggatt tgccaggtg 
      cttcaacca aattgcaca cggtttcca agtgctttt 
      taccgcaag ctcgacaca tcaagcaaa agaaatact 
      gttagccgg attgacaat tgtagattc caagttgtt 
      taagaataa agaaaaata aatttaaac atgatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY