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Gene information
General information
TC014979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5506585..5540959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014979
    iBeetle screen iB_05736

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014979 RA
      MEKDSHYCY EPYQKSNSL KRDTKKKSS ESCRCFGKS 
      RKSRTKAFF TGLATNLGI CILLFGYTL IGSVIFLAI 
      EGGSTFQHS TQILATTSG KKFLNHSAE FKAKNDEAR 
      ARTVENIWD ITVNLNILY RDNWTRLAA QEITRFQDE 
      LLKSLGEEL AAQPVQEKA ERRAGEYEW TFAKAFLYS 
      LTVLTTIGY GSIAPRTTL GKAVTMGYA MLGIPLTLL 
      YLSSIGSIL SRVARGVFS RALCCCLCS NCGYCCYDE 
      KRMAEKERR MKKKRQQME LQQQMALQE PFYVRSNSI 
      HNSLHSPIR DGGVKEIDS LSTDNESKS SMHGLSILA 
      PILLCLAMM FIYICLGTF ALYKLENWS ILDGFYFCF 
      MSLTTIGFG DMVPGSDPF GRESNTTIW FCSIYIMSG 
      MALTAMCFN VVHDEIVHR LKHQEKIAP KVNSASYAD 
      DLNSSDPYN MTS
    CDS sequence
    • >TC014979 RA
      atggagaaa gactctcac tactgctac gaaccttat 
      caaaaaagc aactcttta aaacgcgac acgaagaaa 
      aaatcgtcg gaatcgtgt cgctgtttc ggcaaatcg 
      cgcaaatcg cgcactaaa gccttcttc accggcttg 
      gccaccaac ctagggata tgtattctc ctcttcgga 
      tacactctc atcggttcg gtgattttc ctggccatc 
      gaaggcggt tcaacgttt cagcactcg acccagatt 
      ttggcgacg acatccgga aagaagttt ttgaaccat 
      agtgcggaa tttaaggcg aagaatgac gaggcgagg 
      gcgaggact gtcgagaat atttgggat ataacggtg 
      aatctgaat atcctgtac agggataat tggacgagg 
      ctggcggcg caggagatc acgaggttt caggacgag 
      ctgctgaag agcctgggg gaggagctg gcggcgcag 
      ccggtgcag gagaaggcc gagaggagg gccggggag 
      tacgagtgg acctttgcg aaagcgttt ttatactcg 
      ctgacggtg ctcaccact attggttat gggagcatt 
      gcgcccagg acgacccta ggcaaagcc gtcaccatg 
      ggctacgcc atgctgggc atccccctc actcttcta 
      tacctctcc agcatcggc tcaattctc tctcgtgta 
      gcccgtggt gttttttcg cgcgccctc tgttgctgt 
      ctttgctca aattgcggc tattgttgt tacgacgag 
      aaacgaatg gccgaaaaa gaacgaaga atgaagaaa 
      aagcgccaa caaatggag ctccagcaa caaatggcg 
      ctccaagaa ccattctat gtccgttct aactcgatc 
      cacaacagt ctacactcc cctataagg gacggtggg 
      gtgaaggag attgatagt ttgagtact gacaacgaa 
      tcaaagtcg tcaatgcat ggattgagc attctggcg 
      ccgattctt ctgtgtttg gccatgatg tttatctac 
      atttgttta gggactttc gctttgtac aaattggag 
      aactggtcg atcctggat gggttttac ttctgtttt 
      atgagtctg acgaccatt ggattcggc gatatggtc 
      cctgggagt gacccattt gggcgcgag tcgaacacg 
      accatatgg ttctgctca atttacata atgtcaggg 
      atggcgttg acggcaatg tgcttcaat gtcgtccat 
      gacgaaatc gtccataga ttgaaacat caagagaaa 
      atcgcccca aaagtgaat tcggcaagt tatgccgac 
      gatttgaat tcatcagat ccgtacaac atgacgtca 
      tga
    mRNA sequence
    • >TC014979 RA
      ttcgcatag aaatgtctc aaagttgga ccggagcca 
      acataagcc cgactccgg gccaaacag gggaattac 
      acaattagt ataatgtaa atcggtcca ttaatgttt 
      aaaattcgc tcaaaagtt taatgggcc atatttcac 
      gcaactatc gattcttac agcgcgaca ataagaccg 
      attttttaa acgcaatat ttctgttgc aggcccgag 
      tgatggatt aggtcagct taacccgaa aataactct 
      gtgacttgc cgcgtctaa atctcatca aacccacca 
      taaaatgtg acagtgtga acccgccct aacgccatg 
      gagaaagac tctcactac tgctacgaa ccttatcaa 
      aaaagcaac tctttaaaa cgcgacacg aagaaaaaa 
      tcgtcggaa tcgtgtcgc tgtttcggc aaatcgcgc 
      aaatcgcgc actaaagcc ttcttcacc ggcttggcc 
      accaaccta gggatatgt attctcctc ttcggatac 
      actctcatc ggttcggtg attttcctg gccatcgaa 
      ggcggttca acgtttcag cactcgacc cagattttg 
      gcgacgaca tccggaaag aagtttttg aaccatagt 
      gcggaattt aaggcgaag aatgacgag gcgagggcg 
      aggactgtc gagaatatt tgggatata acggtgaat 
      ctgaatatc ctgtacagg gataattgg acgaggctg 
      gcggcgcag gagatcacg aggtttcag gacgagctg 
      ctgaagagc ctgggggag gagctggcg gcgcagccg 
      gtgcaggag aaggccgag aggagggcc ggggagtac 
      gagtggacc tttgcgaaa gcgttttta tactcgctg 
      acggtgctc accactatt ggttatggg agcattgcg 
      cccaggacg accctaggc aaagccgtc accatgggc 
      tacgccatg ctgggcatc cccctcact cttctatac 
      ctctccagc atcggctca attctctct cgtgtagcc 
      cgtggtgtt ttttcgcgc gccctctgt tgctgtctt 
      tgctcaaat tgcggctat tgttgttac gacgagaaa 
      cgaatggcc gaaaaagaa cgaagaatg aagaaaaag 
      cgccaacaa atggagctc cagcaacaa atggcgctc 
      caagaacca ttctatgtc cgttctaac tcgatccac 
      aacagtcta cactcccct ataagggac ggtggggtg 
      aaggagatt gatagtttg agtactgac aacgaatca 
      aagtcgtca atgcatgga ttgagcatt ctggcgccg 
      attcttctg tgtttggcc atgatgttt atctacatt 
      tgtttaggg actttcgct ttgtacaaa ttggagaac 
      tggtcgatc ctggatggg ttttacttc tgttttatg 
      agtctgacg accattgga ttcggcgat atggtccct 
      gggagtgac ccatttggg cgcgagtcg aacacgacc 
      atatggttc tgctcaatt tacataatg tcagggatg 
      gcgttgacg gcaatgtgc ttcaatgtc gtccatgac 
      gaaatcgtc catagattg aaacatcaa gagaaaatc 
      gccccaaaa gtgaattcg gcaagttat gccgacgat 
      ttgaattca tcagatccg tacaacatg acgtcatga 
      cagttcaat ccgcagaag aacaacacg ttttatcac 
      aggaatttg acagaagag acgctttta agtacgggg 
      actcccacg ctagaaacg ccgaaaata cccgaagtg 
      ttagaaccg gtgatgact ggagtttac actgtgccg 
      gaattaccc gaagagaac gcggaagac aacaccgaa 
      atgtaaaca tatcacgac caaagacaa ttagaattt 
      tcttcccac tcccccgat tataattgt taaactaaa 
      ttaactgtt aaactgtaa ataattaag atgaattac 
      tgatgtcta atagatcaa actgtaatt tcgaatttg 
      taaataaat tagctgtaa tataataat cacgagaca 
      ttctttgta ctctgatga tgaattcct atgtcgtac 
      gaaacgctc tactgtcta ggatgtttt taattctat 
      gtatgtaag actgcggtg aatgtaaaa tttgtataa 
      atgataata aaattcttt attacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY