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Gene information
General information
TC014974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5679591..5680810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014974
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014974 RA
      MEKITNGVI SPPPAPDLN GNTSKGLKN CNGVSKLKD 
      QIMTEDGRV FYPCQFCNK VFPIKKSRT LHIQKCAAK 
      EYILSVGDE GKIPKDNPE KSPELKKID IKQNIVLNE 
      LKTSPKSPP IKLTIKRSP SNGSNHFTV SNSLVLKEK 
      NDTDDFNTI AKRLESNSV TITKQAPKN EQKAPPESD 
      DLNEFTCRE CKKEFKSSL ELLRHGRKV HSYPKVLMA 
      LSEVKKFYA AKNRSECPI CHKPLKTNF RSAFVKHLL 
      THTNEMKYS CKVCNKKFR RSDHMKAHE KRHV
    CDS sequence
    • >TC014974 RA
      atggaaaaa ataacgaac ggggttatt tcaccaccg 
      ccagccccc gacctgaat ggaaacacc tcaaagggg 
      ctcaaaaat tgcaacgga gtgtccaaa ctcaaagac 
      cagatcatg accgaggac ggccgtgtt ttctaccct 
      tgccaattc tgcaacaaa gtcttccct attaaaaaa 
      tcgcgtact ttacacatt cagaaatgc gcggccaaa 
      gagtacatt ttgtcagtc ggggacgag ggtaaaata 
      cccaaagac aatcccgag aaatccccc gaactgaaa 
      aaaatcgat attaagcaa aacattgtc ttgaatgag 
      cttaagact tcgcccaag tcgcccccg attaaatta 
      acgattaag cgcagcccc agcaacggt agcaaccac 
      ttcactgtc tccaactcg cttgtcttg aaggaaaaa 
      aacgacact gacgatttt aacacaatt gccaaaaga 
      ctggaaagt aattctgtg acaataacg aagcaggcc 
      ccgaaaaac gagcaaaaa gcgccccct gaaagcgac 
      gatttgaac gagtttact tgtcgcgag tgtaagaag 
      gagttcaaa tcgtctttg gagttgtta agacatggg 
      cggaaagtc cactcttac ccaaaggtt ctgatggct 
      ttgagtgag gtaaaaaag ttttacgcg gccaaaaat 
      aggtcagag tgcccgatt tgtcataaa cccctcaag 
      acgaatttc cggtctgcg tttgttaaa catctgcta 
      acgcatacg aatgaaatg aagtattcg tgtaaagtc 
      tgtaataaa aagttccgc aggtctgat catatgaaa 
      gcgcacgag aagcggcat gtttag
    mRNA sequence
    • >TC014974 RA
      tcaactgtc acttttacg tttgtttac ttttataat 
      ttttctgtt atttttcgg tttttttcg ttttattct 
      taaatttac cagctttgc tcacataac aatggaaaa 
      aataacgaa cggggttat ttcaccacc gccagcccc 
      cgacctgaa tggaaacac ctcaaaggg gctcaaaaa 
      ttgcaacgg agtgtccaa actcaaaga ccagatcat 
      gaccgagga cggccgtgt tttctaccc ttgccaatt 
      ctgcaacaa agtcttccc tattaaaaa atcgcgtac 
      tttacacat tcagaaatg cgcggccaa agagtacat 
      tttgtcagt cggggacga gggtaaaat acccaaaga 
      caatcccga gaaatcccc cgaactgaa aaaaatcga 
      tattaagca aaacattgt cttgaatga gcttaagac 
      ttcgcccaa gtcgccccc gattaaatt aacgattaa 
      gcgcagccc cagcaacgg tagcaacca cttcactgt 
      ctccaactc gcttgtctt gaaggaaaa aaacgacac 
      tgacgattt taacacaat tgccaaaag actggaaag 
      taattctgt gacaataac gaagcaggc cccgaaaaa 
      cgagcaaaa agcgccccc tgaaagcga cgatttgaa 
      cgagtttac ttgtcgcga gtgtaagaa ggagttcaa 
      atcgtcttt ggagttgtt aagacatgg gcggaaagt 
      ccactctta cccaaaggt tctgatggc tttgagtga 
      ggtaaaaaa gttttacgc ggccaaaaa taggtcaga 
      gtgcccgat ttgtcataa acccctcaa gacgaattt 
      ccggtctgc gtttgttaa acatctgct aacgcatac 
      gaatgaaat gaagtattc gtgtaaagt ctgtaataa 
      aaagttccg caggtctga tcatatgaa agcgcacga 
      gaagcggca tgtttaggg ataagtatt gttacttta 
      tagggatat ttattgttt actcattag actttttaa 
      gccaccagt tatgtattt ttagtgtat cgttttgtt 
      tcgagtgtt gcattacta tacatttta tcattgtaa 
      tgtttttct tcaaagtat gaatcgagt tgagattta 
      agcggcaaa atttattgt gccggcatt ttttgacaa 
      atatatttt ttccacaag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY