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Gene information
General information
TC014973 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5691246..5692750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014973
    iBeetle screen iB_05733

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 33.33% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014973 RA
      MRPFSKMNK LFLGVCAAS GAATYYFLN GSNKTVYAS 
      WTSNYEPSP HARWDDNWD HRSPKFLSH PKYSKDENN 
      NQIHSEIEL NKAKSVRHL ILIRHGQYN VNGQTDEDR 
      TLTKLGKIQ AEYTGKRLK ELGLPYTKI IKSTMSRAQ 
      ETGSIISVS LPDVPVKNC DLLREGAPI PPEPPIGSW 
      KQEMYKFFV DGARIEAAF RRYFHRAAP QQKEDSYTV 
      LVCHANVIR YFVCRALQL PAEAWLRMS LNHASITWI 
      SITPSGRVV LRCLGDSGH MPPDVVTSR
    CDS sequence
    • >TC014973 RA
      atgcgccca ttttcgaag atgaacaag ctgtttctg 
      ggtgtgtgt gcggcttcg ggggccgca acttactac 
      tttttgaac ggttcaaat aagacagtt tacgcttct 
      tggacgtcg aattacgag ccttcgcca catgccaga 
      tgggacgat aattgggac catcgctcg ccgaaattt 
      ttatcacac ccgaaatac tccaaagat gagaataac 
      aatcagatt catagcgaa attgaatta aataaagct 
      aagtcagtc cgacatttg attctgata cgtcatggt 
      caatacaat gttaatggc caaacggac gaagaccgg 
      actttaaca aaactaggg aaaattcaa gctgaatat 
      acggggaaa aggctcaaa gagttgggt ctaccgtat 
      acaaaaata atcaaatcg acaatgtct cgtgcgcaa 
      gagactggt tcaattatc tcagtttct ctccccgac 
      gttccggtt aaaaactgc gatttgttg cgtgaaggg 
      gcccccata cctccggag cccccgatc gggtcatgg 
      aagcaagaa atgtataag tttttcgtt gatggggca 
      cgaattgag gccgctttt cgtagatat ttccatagg 
      gctgcgccc caacaaaag gaagactcg tatactgtt 
      ttagtgtgt catgctaat gtgattcgg tattttgtg 
      tgcagggca ctgcaatta ccagccgag gcatggttg 
      aggatgtct ttgaaccat gctagtatt acttggatt 
      agtattact ccaagtggg cgcgtggtc ttaaggtgt 
      ttgggcgat agcgggcac atgccgcca gatgtcgtc 
      actagtcga taa
    mRNA sequence
    • >TC014973 RA
      tgtcactgt gacagcttt aacctcact ttgcaaatt 
      gagcttcac atttcccga taagaatta tcacttaaa 
      ccgtaaaca aacattgaa ttttctaca attcatcca 
      acatgcgcc cattttcga agatgaaca agctgtttc 
      tgggtgtgt gtgcggctt cgggggccg caacttact 
      actttttga acggttcaa ataagacag tttacgctt 
      cttggacgt cgaattacg agccttcgc cacatgcca 
      gatgggacg ataattggg accatcgct cgccgaaat 
      ttttatcac acccgaaat actccaaag atgagaata 
      acaatcaga ttcatagcg aaattgaat taaataaag 
      ctaagtcag tccgacatt tgattctga tacgtcatg 
      gtcaataca atgttaatg gccaaacgg acgaagacc 
      ggactttaa caaaactag ggaaaattc aagctgaat 
      atacgggga aaaggctca aagagttgg gtctaccgt 
      atacaaaaa taatcaaat cgacaatgt ctcgtgcgc 
      aagagactg gttcaatta tctcagttt ctctccccg 
      acgttccgg ttaaaaact gcgatttgt tgcgtgaag 
      gggccccca tacctccgg agcccccga tcgggtcat 
      ggaagcaag aaatgtata agtttttcg ttgatgggg 
      cacgaattg aggccgctt ttcgtagat atttccata 
      gggctgcgc cccaacaaa aggaagact cgtatactg 
      ttttagtgt gtcatgcta atgtgattc ggtattttg 
      tgtgcaggg cactgcaat taccagccg aggcatggt 
      tgaggatgt ctttgaacc atgctagta ttacttgga 
      ttagtatta ctccaagtg ggcgcgtgg tcttaaggt 
      gtttgggcg atagcgggc acatgccgc cagatgtcg 
      tcactagtc gataaattt ttatcattc aataataag 
      cgcaaaaac acatttttt ttattcatg taaatgtat 
      ttttaagta ttatttttt tgcctggaa tcacttttt 
      atttttgat tttgtaaca ccaaattaa agaatacaa 
      aactacaat tagttgcct cttttaaaa tgactaaaa 
      agctctatc ggaatgggg aaaacaatc gttgagttt 
      ttctcagct gaaatattg ctcagtgtt tgtaaatac 
      ctcaactga aacaaaaaa aaacaattc attagttca 
      ctacaactt cggaaaatt gtacactca ctgccataa 
      agaatgtca cccctataa accccgact gatagcaaa 
      gggcccaac tcagactct gggctcaaa acgtcaata 
      aacattctt tctgagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY