Status
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Gene information
General information
TC014963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5827904..5829882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014963
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05731

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014963 RA
      MAVLETSMT PKTRENAKK PSKNNKKQA KKVLLVAQE 
      LKLARVLAG NNKTARDRA LKSLRKWFQ NRSSAIPFT 
      EDDFLRLWK GLFYSMWMS DKPLVQEEC AENIASLIH 
      ALPVEGALL FYKCGMTIL MNEWFGIDQ LRLDKFLMF 
      VRRLLRQAF LVLKNQNWG RDSAQTFTK TLSETILDP 
      PRHKPMGLL MHFIEIYLE ELSKVSEGQ LPPQLMTEL 
      LAPFVRQLS FSDDARVID HIRKHIFIY LIRQSDLGL 
      EYDEKYKAW RALRFPGTI DAMQKIEVS DDEGGEEEP 
      PQDKVFDPR AGKVNVDLP QLKFHPRAV SKALLECRF 
      SKETNSKSR KCLTELAEH FRKLASGTY PLGVKKVKL 
      ASDDYDTNI RKAVNRLVK FDKKIKGTK NRKRKLQSD 
      TIELRGGKK IKIDEKLRE KFQDDLVRV MKKKRKLVD 
      KPESPPEKQ SKLQVESIF KRNSGTWLV TKPPQEEAQ 
      KAPQELFPK NKWDESNHA TEEVEKSLK SKRLSLPTK 
      LIKNPFANS TPVKKVKIN TKLNRSQDV QEHHAQILS 
      SPQIPYDAN KKPAKPLLK TPTLKSPID PFYAL
    CDS sequence
    • >TC014963 RA
      atggcggtg cttgaaacg agtatgaca cctaagaca 
      cgtgaaaat gcgaaaaaa ccgtcaaaa aataacaaa 
      aaacaagcc aaaaaggtg cttttagtg gcacaagag 
      ttaaaactc gcccgcgtt ttggccgga aataacaaa 
      acagcgcga gatagggcg ctgaaaagc ttacgaaag 
      tggtttcaa aacaggtcg tctgcaatt cctttcact 
      gaagatgat ttcttacga ctctggaag ggtttgttc 
      tattcaatg tggatgtcg gacaagcct ttggttcaa 
      gaagaatgt gccgagaat attgcaagt ttgattcac 
      gcgttgcca gtcgagggg gccttgttg ttttacaag 
      tgtgggatg acgatttta atgaatgaa tggtttggg 
      attgaccaa cttcgacta gacaagttt ttgatgttt 
      gtgcggcgg cttttgaga caggctttt ctggtgttg 
      aaaaaccaa aattggggg cgtgattca gcccagact 
      tttactaaa acactctca gagacaatt ctggacccc 
      ccacgacac aaaccaatg gggctgctt atgcatttc 
      attgaaatc tatctcgag gaattatcc aaagtctca 
      gagggacaa ttgccccca cagttaatg acagagctg 
      ttagcccct tttgtgaga cagttgtcg tttagtgac 
      gatgctaga gtcattgat catatcagg aaacacatt 
      tttatttat ttgataaga cagtcagat ttgggcttg 
      gaatatgat gagaaatac aaagcttgg cgcgctttg 
      aggtttcct gggactata gacgccatg caaaaaatc 
      gaagtctcg gacgacgaa gggggcgag gaggagccc 
      ccacaagac aaagttttc gaccctcgg gcgggtaaa 
      gtcaacgtg gacttgcca caattgaaa ttccaccca 
      agagctgtg tctaaagct ttactagag tgtaggttt 
      tcaaaagag accaattcc aagtcacgg aagtgtcta 
      actgaacta gcagaacat tttaggaaa ttagcatca 
      gggacttat ccccttggt gtgaaaaaa gtaaaatta 
      gcatctgat gattacgat acgaatatt agaaaagca 
      gttaatcgt ttagtaaag ttcgataag aaaatcaaa 
      ggcacgaaa aaccgcaaa cgaaagctc caaagtgac 
      acgattgag ttacgaggc gggaaaaaa atcaaaatc 
      gacgaaaaa ctgcgcgaa aaattccaa gacgattta 
      gttcgagtc atgaaaaag aaacgcaag ttggtcgac 
      aagcccgaa agcccgcct gagaaacag tccaaacta 
      caagttgaa tcaattttc aaacgtaat tccgggacg 
      tggttggtc acaaaaccc cctcaagaa gaagcgcaa 
      aaagcgcca caagaacta tttccgaaa aataaatgg 
      gatgagagc aaccatgca actgaagag gtggagaaa 
      agcttgaag agtaaaaga ttgagtttg cctacgaaa 
      ctaatcaaa aacccattt gccaactcg actccggtc 
      aagaaagtc aaaatcaat accaagctc aataggtca 
      caagacgtg caagagcac catgcccag attttgagc 
      tcgccacaa attccatac gacgccaac aagaagccg 
      gcaaagccg ttgctgaaa actccgaca ctgaagagc 
      cccattgat cctttctat gctttgtaa
    mRNA sequence
    • >TC014963 RA
      ttcgtctaa cggttggtg tgtcgagtg gtgcaaaaa 
      tggcggtgc ttgaaacga gtatgacac ctaagacac 
      gtgaaaatg cgaaaaaac cgtcaaaaa ataacaaaa 
      aacaagcca aaaaggtgc ttttagtgg cacaagagt 
      taaaactcg cccgcgttt tggccggaa ataacaaaa 
      cagcgcgag atagggcgc tgaaaagct tacgaaagt 
      ggtttcaaa acaggtcgt ctgcaattc ctttcactg 
      aagatgatt tcttacgac tctggaagg gtttgttct 
      attcaatgt ggatgtcgg acaagcctt tggttcaag 
      aagaatgtg ccgagaata ttgcaagtt tgattcacg 
      cgttgccag tcgaggggg ccttgttgt tttacaagt 
      gtgggatga cgattttaa tgaatgaat ggtttggga 
      ttgaccaac ttcgactag acaagtttt tgatgtttg 
      tgcggcggc ttttgagac aggcttttc tggtgttga 
      aaaaccaaa attgggggc gtgattcag cccagactt 
      ttactaaaa cactctcag agacaattc tggaccccc 
      cacgacaca aaccaatgg ggctgctta tgcatttca 
      ttgaaatct atctcgagg aattatcca aagtctcag 
      agggacaat tgcccccac agttaatga cagagctgt 
      tagcccctt ttgtgagac agttgtcgt ttagtgacg 
      atgctagag tcattgatc atatcagga aacacattt 
      ttatttatt tgataagac agtcagatt tgggcttgg 
      aatatgatg agaaataca aagcttggc gcgctttga 
      ggtttcctg ggactatag acgccatgc aaaaaatcg 
      aagtctcgg acgacgaag ggggcgagg aggagcccc 
      cacaagaca aagttttcg accctcggg cgggtaaag 
      tcaacgtgg acttgccac aattgaaat tccacccaa 
      gagctgtgt ctaaagctt tactagagt gtaggtttt 
      caaaagaga ccaattcca agtcacgga agtgtctaa 
      ctgaactag cagaacatt ttaggaaat tagcatcag 
      ggacttatc cccttggtg tgaaaaaag taaaattag 
      catctgatg attacgata cgaatatta gaaaagcag 
      ttaatcgtt tagtaaagt tcgataaga aaatcaaag 
      gcacgaaaa accgcaaac gaaagctcc aaagtgaca 
      cgattgagt tacgaggcg ggaaaaaaa tcaaaatcg 
      acgaaaaac tgcgcgaaa aattccaag acgatttag 
      ttcgagtca tgaaaaaga aacgcaagt tggtcgaca 
      agcccgaaa gcccgcctg agaaacagt ccaaactac 
      aagttgaat caattttca aacgtaatt ccgggacgt 
      ggttggtca caaaacccc ctcaagaag aagcgcaaa 
      aagcgccac aagaactat ttccgaaaa ataaatggg 
      atgagagca accatgcaa ctgaagagg tggagaaaa 
      gcttgaaga gtaaaagat tgagtttgc ctacgaaac 
      taatcaaaa acccatttg ccaactcga ctccggtca 
      agaaagtca aaatcaata ccaagctca ataggtcac 
      aagacgtgc aagagcacc atgcccaga ttttgagct 
      cgccacaaa ttccatacg acgccaaca agaagccgg 
      caaagccgt tgctgaaaa ctccgacac tgaagagcc 
      ccattgatc ctttctatg ctttgtaaa tctattttt 
      tacgtaata aaattgata attgtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY