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Gene information
General information
TC014963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5827904..5829882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014963
    iBeetle screen iB_05731

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014963 RA
      MAVLETSMT PKTRENAKK PSKNNKKQA KKVLLVAQE 
LKLARVLAG NNKTARDRA LKSLRKWFQ NRSSAIPFT 
EDDFLRLWK GLFYSMWMS DKPLVQEEC AENIASLIH 
ALPVEGALL FYKCGMTIL MNEWFGIDQ LRLDKFLMF 
VRRLLRQAF LVLKNQNWG RDSAQTFTK TLSETILDP 
PRHKPMGLL MHFIEIYLE ELSKVSEGQ LPPQLMTEL 
LAPFVRQLS FSDDARVID HIRKHIFIY LIRQSDLGL 
EYDEKYKAW RALRFPGTI DAMQKIEVS DDEGGEEEP 
PQDKVFDPR AGKVNVDLP QLKFHPRAV SKALLECRF 
SKETNSKSR KCLTELAEH FRKLASGTY PLGVKKVKL 
ASDDYDTNI RKAVNRLVK FDKKIKGTK NRKRKLQSD 
TIELRGGKK IKIDEKLRE KFQDDLVRV MKKKRKLVD 
KPESPPEKQ SKLQVESIF KRNSGTWLV TKPPQEEAQ 
KAPQELFPK NKWDESNHA TEEVEKSLK SKRLSLPTK 
LIKNPFANS TPVKKVKIN TKLNRSQDV QEHHAQILS 
SPQIPYDAN KKPAKPLLK TPTLKSPID PFYAL
    CDS sequence
    • >TC014963 RA
      atggcggtg cttgaaacg agtatgaca cctaagaca 
cgtgaaaat gcgaaaaaa ccgtcaaaa aataacaaa 
aaacaagcc aaaaaggtg cttttagtg gcacaagag 
ttaaaactc gcccgcgtt ttggccgga aataacaaa 
acagcgcga gatagggcg ctgaaaagc ttacgaaag 
tggtttcaa aacaggtcg tctgcaatt cctttcact 
gaagatgat ttcttacga ctctggaag ggtttgttc 
tattcaatg tggatgtcg gacaagcct ttggttcaa 
gaagaatgt gccgagaat attgcaagt ttgattcac 
gcgttgcca gtcgagggg gccttgttg ttttacaag 
tgtgggatg acgatttta atgaatgaa tggtttggg 
attgaccaa cttcgacta gacaagttt ttgatgttt 
gtgcggcgg cttttgaga caggctttt ctggtgttg 
aaaaaccaa aattggggg cgtgattca gcccagact 
tttactaaa acactctca gagacaatt ctggacccc 
ccacgacac aaaccaatg gggctgctt atgcatttc 
attgaaatc tatctcgag gaattatcc aaagtctca 
gagggacaa ttgccccca cagttaatg acagagctg 
ttagcccct tttgtgaga cagttgtcg tttagtgac 
gatgctaga gtcattgat catatcagg aaacacatt 
tttatttat ttgataaga cagtcagat ttgggcttg 
gaatatgat gagaaatac aaagcttgg cgcgctttg 
aggtttcct gggactata gacgccatg caaaaaatc 
gaagtctcg gacgacgaa gggggcgag gaggagccc 
ccacaagac aaagttttc gaccctcgg gcgggtaaa 
gtcaacgtg gacttgcca caattgaaa ttccaccca 
agagctgtg tctaaagct ttactagag tgtaggttt 
tcaaaagag accaattcc aagtcacgg aagtgtcta 
actgaacta gcagaacat tttaggaaa ttagcatca 
gggacttat ccccttggt gtgaaaaaa gtaaaatta 
gcatctgat gattacgat acgaatatt agaaaagca 
gttaatcgt ttagtaaag ttcgataag aaaatcaaa 
ggcacgaaa aaccgcaaa cgaaagctc caaagtgac 
acgattgag ttacgaggc gggaaaaaa atcaaaatc 
gacgaaaaa ctgcgcgaa aaattccaa gacgattta 
gttcgagtc atgaaaaag aaacgcaag ttggtcgac 
aagcccgaa agcccgcct gagaaacag tccaaacta 
caagttgaa tcaattttc aaacgtaat tccgggacg 
tggttggtc acaaaaccc cctcaagaa gaagcgcaa 
aaagcgcca caagaacta tttccgaaa aataaatgg 
gatgagagc aaccatgca actgaagag gtggagaaa 
agcttgaag agtaaaaga ttgagtttg cctacgaaa 
ctaatcaaa aacccattt gccaactcg actccggtc 
aagaaagtc aaaatcaat accaagctc aataggtca 
caagacgtg caagagcac catgcccag attttgagc 
tcgccacaa attccatac gacgccaac aagaagccg 
gcaaagccg ttgctgaaa actccgaca ctgaagagc 
cccattgat cctttctat gctttgtaa
    mRNA sequence
    • >TC014963 RA
      ttcgtctaa cggttggtg tgtcgagtg gtgcaaaaa 
tggcggtgc ttgaaacga gtatgacac ctaagacac 
gtgaaaatg cgaaaaaac cgtcaaaaa ataacaaaa 
aacaagcca aaaaggtgc ttttagtgg cacaagagt 
taaaactcg cccgcgttt tggccggaa ataacaaaa 
cagcgcgag atagggcgc tgaaaagct tacgaaagt 
ggtttcaaa acaggtcgt ctgcaattc ctttcactg 
aagatgatt tcttacgac tctggaagg gtttgttct 
attcaatgt ggatgtcgg acaagcctt tggttcaag 
aagaatgtg ccgagaata ttgcaagtt tgattcacg 
cgttgccag tcgaggggg ccttgttgt tttacaagt 
gtgggatga cgattttaa tgaatgaat ggtttggga 
ttgaccaac ttcgactag acaagtttt tgatgtttg 
tgcggcggc ttttgagac aggcttttc tggtgttga 
aaaaccaaa attgggggc gtgattcag cccagactt 
ttactaaaa cactctcag agacaattc tggaccccc 
cacgacaca aaccaatgg ggctgctta tgcatttca 
ttgaaatct atctcgagg aattatcca aagtctcag 
agggacaat tgcccccac agttaatga cagagctgt 
tagcccctt ttgtgagac agttgtcgt ttagtgacg 
atgctagag tcattgatc atatcagga aacacattt 
ttatttatt tgataagac agtcagatt tgggcttgg 
aatatgatg agaaataca aagcttggc gcgctttga 
ggtttcctg ggactatag acgccatgc aaaaaatcg 
aagtctcgg acgacgaag ggggcgagg aggagcccc 
cacaagaca aagttttcg accctcggg cgggtaaag 
tcaacgtgg acttgccac aattgaaat tccacccaa 
gagctgtgt ctaaagctt tactagagt gtaggtttt 
caaaagaga ccaattcca agtcacgga agtgtctaa 
ctgaactag cagaacatt ttaggaaat tagcatcag 
ggacttatc cccttggtg tgaaaaaag taaaattag 
catctgatg attacgata cgaatatta gaaaagcag 
ttaatcgtt tagtaaagt tcgataaga aaatcaaag 
gcacgaaaa accgcaaac gaaagctcc aaagtgaca 
cgattgagt tacgaggcg ggaaaaaaa tcaaaatcg 
acgaaaaac tgcgcgaaa aattccaag acgatttag 
ttcgagtca tgaaaaaga aacgcaagt tggtcgaca 
agcccgaaa gcccgcctg agaaacagt ccaaactac 
aagttgaat caattttca aacgtaatt ccgggacgt 
ggttggtca caaaacccc ctcaagaag aagcgcaaa 
aagcgccac aagaactat ttccgaaaa ataaatggg 
atgagagca accatgcaa ctgaagagg tggagaaaa 
gcttgaaga gtaaaagat tgagtttgc ctacgaaac 
taatcaaaa acccatttg ccaactcga ctccggtca 
agaaagtca aaatcaata ccaagctca ataggtcac 
aagacgtgc aagagcacc atgcccaga ttttgagct 
cgccacaaa ttccatacg acgccaaca agaagccgg 
caaagccgt tgctgaaaa ctccgacac tgaagagcc 
ccattgatc ctttctatg ctttgtaaa tctattttt 
tacgtaata aaattgata attgtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY