| General information | |||
| TC014963 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5827904..5829882 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014963 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05731 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC014963 RA
MAVLETSMT PKTRENAKK PSKNNKKQA KKVLLVAQE LKLARVLAG NNKTARDRA LKSLRKWFQ NRSSAIPFT EDDFLRLWK GLFYSMWMS DKPLVQEEC AENIASLIH ALPVEGALL FYKCGMTIL MNEWFGIDQ LRLDKFLMF VRRLLRQAF LVLKNQNWG RDSAQTFTK TLSETILDP PRHKPMGLL MHFIEIYLE ELSKVSEGQ LPPQLMTEL LAPFVRQLS FSDDARVID HIRKHIFIY LIRQSDLGL EYDEKYKAW RALRFPGTI DAMQKIEVS DDEGGEEEP PQDKVFDPR AGKVNVDLP QLKFHPRAV SKALLECRF SKETNSKSR KCLTELAEH FRKLASGTY PLGVKKVKL ASDDYDTNI RKAVNRLVK FDKKIKGTK NRKRKLQSD TIELRGGKK IKIDEKLRE KFQDDLVRV MKKKRKLVD KPESPPEKQ SKLQVESIF KRNSGTWLV TKPPQEEAQ KAPQELFPK NKWDESNHA TEEVEKSLK SKRLSLPTK LIKNPFANS TPVKKVKIN TKLNRSQDV QEHHAQILS SPQIPYDAN KKPAKPLLK TPTLKSPID PFYAL
- >TC014963 RA
atggcggtg cttgaaacg agtatgaca cctaagaca cgtgaaaat gcgaaaaaa ccgtcaaaa aataacaaa aaacaagcc aaaaaggtg cttttagtg gcacaagag ttaaaactc gcccgcgtt ttggccgga aataacaaa acagcgcga gatagggcg ctgaaaagc ttacgaaag tggtttcaa aacaggtcg tctgcaatt cctttcact gaagatgat ttcttacga ctctggaag ggtttgttc tattcaatg tggatgtcg gacaagcct ttggttcaa gaagaatgt gccgagaat attgcaagt ttgattcac gcgttgcca gtcgagggg gccttgttg ttttacaag tgtgggatg acgatttta atgaatgaa tggtttggg attgaccaa cttcgacta gacaagttt ttgatgttt gtgcggcgg cttttgaga caggctttt ctggtgttg aaaaaccaa aattggggg cgtgattca gcccagact tttactaaa acactctca gagacaatt ctggacccc ccacgacac aaaccaatg gggctgctt atgcatttc attgaaatc tatctcgag gaattatcc aaagtctca gagggacaa ttgccccca cagttaatg acagagctg ttagcccct tttgtgaga cagttgtcg tttagtgac gatgctaga gtcattgat catatcagg aaacacatt tttatttat ttgataaga cagtcagat ttgggcttg gaatatgat gagaaatac aaagcttgg cgcgctttg aggtttcct gggactata gacgccatg caaaaaatc gaagtctcg gacgacgaa gggggcgag gaggagccc ccacaagac aaagttttc gaccctcgg gcgggtaaa gtcaacgtg gacttgcca caattgaaa ttccaccca agagctgtg tctaaagct ttactagag tgtaggttt tcaaaagag accaattcc aagtcacgg aagtgtcta actgaacta gcagaacat tttaggaaa ttagcatca gggacttat ccccttggt gtgaaaaaa gtaaaatta gcatctgat gattacgat acgaatatt agaaaagca gttaatcgt ttagtaaag ttcgataag aaaatcaaa ggcacgaaa aaccgcaaa cgaaagctc caaagtgac acgattgag ttacgaggc gggaaaaaa atcaaaatc gacgaaaaa ctgcgcgaa aaattccaa gacgattta gttcgagtc atgaaaaag aaacgcaag ttggtcgac aagcccgaa agcccgcct gagaaacag tccaaacta caagttgaa tcaattttc aaacgtaat tccgggacg tggttggtc acaaaaccc cctcaagaa gaagcgcaa aaagcgcca caagaacta tttccgaaa aataaatgg gatgagagc aaccatgca actgaagag gtggagaaa agcttgaag agtaaaaga ttgagtttg cctacgaaa ctaatcaaa aacccattt gccaactcg actccggtc aagaaagtc aaaatcaat accaagctc aataggtca caagacgtg caagagcac catgcccag attttgagc tcgccacaa attccatac gacgccaac aagaagccg gcaaagccg ttgctgaaa actccgaca ctgaagagc cccattgat cctttctat gctttgtaa
- >TC014963 RA
ttcgtctaa cggttggtg tgtcgagtg gtgcaaaaa tggcggtgc ttgaaacga gtatgacac ctaagacac gtgaaaatg cgaaaaaac cgtcaaaaa ataacaaaa aacaagcca aaaaggtgc ttttagtgg cacaagagt taaaactcg cccgcgttt tggccggaa ataacaaaa cagcgcgag atagggcgc tgaaaagct tacgaaagt ggtttcaaa acaggtcgt ctgcaattc ctttcactg aagatgatt tcttacgac tctggaagg gtttgttct attcaatgt ggatgtcgg acaagcctt tggttcaag aagaatgtg ccgagaata ttgcaagtt tgattcacg cgttgccag tcgaggggg ccttgttgt tttacaagt gtgggatga cgattttaa tgaatgaat ggtttggga ttgaccaac ttcgactag acaagtttt tgatgtttg tgcggcggc ttttgagac aggcttttc tggtgttga aaaaccaaa attgggggc gtgattcag cccagactt ttactaaaa cactctcag agacaattc tggaccccc cacgacaca aaccaatgg ggctgctta tgcatttca ttgaaatct atctcgagg aattatcca aagtctcag agggacaat tgcccccac agttaatga cagagctgt tagcccctt ttgtgagac agttgtcgt ttagtgacg atgctagag tcattgatc atatcagga aacacattt ttatttatt tgataagac agtcagatt tgggcttgg aatatgatg agaaataca aagcttggc gcgctttga ggtttcctg ggactatag acgccatgc aaaaaatcg aagtctcgg acgacgaag ggggcgagg aggagcccc cacaagaca aagttttcg accctcggg cgggtaaag tcaacgtgg acttgccac aattgaaat tccacccaa gagctgtgt ctaaagctt tactagagt gtaggtttt caaaagaga ccaattcca agtcacgga agtgtctaa ctgaactag cagaacatt ttaggaaat tagcatcag ggacttatc cccttggtg tgaaaaaag taaaattag catctgatg attacgata cgaatatta gaaaagcag ttaatcgtt tagtaaagt tcgataaga aaatcaaag gcacgaaaa accgcaaac gaaagctcc aaagtgaca cgattgagt tacgaggcg ggaaaaaaa tcaaaatcg acgaaaaac tgcgcgaaa aattccaag acgatttag ttcgagtca tgaaaaaga aacgcaagt tggtcgaca agcccgaaa gcccgcctg agaaacagt ccaaactac aagttgaat caattttca aacgtaatt ccgggacgt ggttggtca caaaacccc ctcaagaag aagcgcaaa aagcgccac aagaactat ttccgaaaa ataaatggg atgagagca accatgcaa ctgaagagg tggagaaaa gcttgaaga gtaaaagat tgagtttgc ctacgaaac taatcaaaa acccatttg ccaactcga ctccggtca agaaagtca aaatcaata ccaagctca ataggtcac aagacgtgc aagagcacc atgcccaga ttttgagct cgccacaaa ttccatacg acgccaaca agaagccgg caaagccgt tgctgaaaa ctccgacac tgaagagcc ccattgatc ctttctatg ctttgtaaa tctattttt tacgtaata aaattgata attgtac
