Status
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Gene information
General information
TC014955 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7348161..7360477
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014955
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05729

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014955 RA
      MHSLLTFVL SPDNVRRLG PQNAKEVEK LTLKNTEYL 
      FGDTPVDKQ LKRNYVSLG VYNDLEELA GVICFNCFP 
      NIPALPPWE WAYWLHNKY HIDNMTPMT TLWIHYAVW 
      DRRYTTLFF KPILHSLFL TYEPIDTVC MVCPPGIQR 
      LDFIDEYTT RVLPYGFTN PSKVQTIYV AKKVDFVAH 
      YVIRRAVEE DNDDLVAII PKIIEETYG QYYISELLS 
      NLDESGRQI IVAEHRGCA VGVLCLNEE VNYDLLNEE 
      FELVPFNGL RKHDADDEV LKTQVLSAT AIPVLEDST 
      ERLSNTSLQ EETREESAA EQDDQDNVS DDEFSLVNL 
      SSSLIFNFL YEEDETSDH FFMSESETS IESHFSAVV 
      LNEEYEDHL YNADRSGGG GDVEGDEKR IPRRKESKL 
      DNITIPTYY GEPNAFALE ICTAMDDHE NCLYLLFQA 
      AFECFPDRE YLIVSLPTE TQMCYLKKH FARVTPRPN 
      SVFNHELYV LHKSAILGT LWARVAVSE DKPLVSELV 
      ATLSKPSIV EDLFKEAVD SKMGQFKAY TFFCEEQMI 
      GCAIVSEEE EHEYLELHY NVDPWLSPK ETRFGTYGS 
      LEQFLLSPI FQRHINFFT RELHRLSDF DVLFYRLKR 
      YDYIYSHRS LPIVNAIIN MIPVLPNRV PEYSKQITD 
      EFLLPTCLQ KHHQPFGLY LSSLRLSSM PKIEINQKM 
      VIIGAGLTT ISFLESLIF GRNPNYLIV FTNITVVSP 
      HGVTYHKPP SQVREMVFV NDGHVDRRY IDAISLKTY 
      VHVVTGVMT SINRKEKYV GLNDESYLP YDYLFLMCG 
      EQFQKPPLR KKEVVKKRT EFPQNVFII NTEVDAGNA 
      FNALKSLTS GQKQGNMVV FGHYLEACC CLAALLEFG 
      VAGSNLVYI DPNPDSSLR QDRHEHLTQ FFNDSAVET 
      AVLDEILYQ GITIYQDFN FVEWHLDST KNFITSAKF 
      ESKYKFVEI ELSALFIYH SKGVSARTF QAIARSGLV 
      FNGRLVIDK DYQTNDPSI YGAGPLTMY SKKYYAEHL 
      SHKYYNPRE IGYNLGSAI RKKLVPEQF WDPPVKETL 
      IHQPVVQIC KFPGNLCYL NVFKAGRPV PLDVATNKC 
      NYGRVLKTG DCKKLDKQG YFRLHLNQF QTVETITCL 
      AKFDLKVHN LINLWGKHE ILLNHLLLR YDLGMIPDM 
      FQFFDEPWA CAIYYRDFP RVIDEISEL LLTAETNEG 
      VKLVDEVRA QLEKNDWNP LTKIQTDEI EEMCRDTSL 
      IPLIERKVL DFLHDNFMY LPMYVHQLL IRWILHQIQ 
      TSPLFVKTT FISNTRKSF SPGIPFLMR PTQSRME
    CDS sequence
    • >TC014955 RA
      atgcacagt ttactgact tttgtccta tcgcccgat 
      aatgtgaga cgtcttgga ccacaaaac gccaaagaa 
      gtggaaaaa ttgacattg aaaaatacc gagtatttg 
      ttcggggac acgccggtg gacaaacaa cttaagcgg 
      aattatgtc tccttgggg gtttacaac gacttggag 
      gagttggct ggagtgata tgttttaat tgttttccc 
      aacatcccg gcgctaccg ccatgggag tgggcctat 
      tggctgcac aacaagtac cacattgat aacatgaca 
      cccatgacc acgctgtgg atacactac gcggtttgg 
      gaccgcagg tacaccaca ctgttcttc aaacccatc 
      ctgcattca ctcttcctg acctacgaa cccatcgac 
      accgtctgt atggtctgt ccgcccgga atccagcgt 
      ctggacttc attgatgag tacaccaca agggtacta 
      ccatatggt ttcacaaac ccatcaaaa gtccagacc 
      atctacgta gcaaaaaaa gtcgatttt gtggctcac 
      tacgtcata cggcgcgct gttgaagaa gacaacgac 
      gacctcgtg gccattatc ccaaaaatc atcgaagag 
      acctatggc cagtactac atttccgag cttttgagc 
      aacttggac gagtctgga cgccagatc attgtggcg 
      gagcaccga gggtgcgct gtaggtgtc ttgtgtttg 
      aacgaagag gtcaactat gacctcctg aacgaagag 
      tttgagttg gtaccgttc aacggtttg aggaaacac 
      gatgctgac gatgaagta ctcaaaacg caagtcttg 
      agcgcgact gcaatccca gttttggaa gatagtacc 
      gagcgtttg tccaatacc agcctccag gaggagacg 
      cgcgaagag tcagctgcg gagcaagac gaccaagac 
      aacgtctcc gacgatgag ttttctcta gtcaacttg 
      tcgtcgtcc cttattttc aatttcttg tacgaagag 
      gacgaaact agtgatcac ttttttatg tcggagagt 
      gaaactagt atcgaaagc cacttcagt gctgttgtt 
      ctgaacgaa gagtacgag gatcatttg tataacgct 
      gatcggagt gggggtggg ggtgatgtc gaaggggat 
      gaaaaacgc atccctcga cggaaagaa tccaaactg 
      gataatatc acaatcccg acgtattat ggcgaaccg 
      aatgctttc gctcttgaa atttgtaca gcgatggat 
      gatcatgag aactgtctt tatttacta ttccaggcg 
      gcgtttgag tgttttcca gatagggag tatctgatt 
      gtttcgtta ccgaccgag acacagatg tgttatttg 
      aagaaacac tttgcaagg gtcacaccg agacccaac 
      tcggtgttt aatcacgag ttgtatgtt ttgcacaag 
      agtgcgatt ttggggact ttgtgggca agagtggca 
      gtttcggag gataagcct ttggttagt gaattggtg 
      gcgactttg agtaagcca tcaattgtg gaagattta 
      ttcaaggaa gcggtggat agtaagatg gggcagttt 
      aaggcctat acgtttttc tgcgaagag cagatgatc 
      gggtgtgcg atcgtcagc gaagaagaa gaacacgaa 
      taccttgaa ctccactac aacgtcgat ccttggcta 
      agccccaaa gaaacacgt tttgggacc tacggctct 
      ttagaacaa ttcctccta tctccaatt tttcaacgc 
      catatcaac ttcttcact cgcgaattg caccgtttg 
      agcgacttt gacgtcctg ttttaccga ctcaaacgc 
      tacgactac atctacagt cacaggagc ctccctatc 
      gtcaacgcc atcataaac atgatcccc gtactcccg 
      aatcgagtc cccgaatac agcaaacaa ataaccgac 
      gaatttctc cttcccacg tgtttgcaa aaacaccac 
      caaccgttc ggactctac ctatcatca cttcgccta 
      agcagcatg cccaagatc gaaatcaac caaaaaatg 
      gtcattata ggtgcagga cttacaaca atttcgttt 
      ctcgaatcg ttgattttt ggtcgtaac cccaattat 
      ttgatcgtt ttcacgaac ataacagta gtgagtcca 
      cacggggtg acctaccat aagcctcca agccaggtc 
      agggagatg gtttttgtg aacgatggt cacgtcgat 
      cgccgatat atcgatgca atttcgtta aaaacctac 
      gtccatgtt gtgacaggt gttatgact tcaattaac 
      cgcaaggaa aagtacgtg ggtttgaac gacgagtcg 
      taccttccg tacgattat ctcttcctg atgtgcggt 
      gagcagttc cagaaacca ccactcagg aagaaagaa 
      gtcgtgaag aagcgaaca gagttcccg cagaacgtc 
      ttcataatt aacacagaa gtggacgct gggaacgcc 
      ttcaatgcg ctgaaaagc ctaacttcg gggcaaaag 
      cagggcaat atggtggtt tttggtcat tatctggaa 
      gcttgttgt tgtttggct gcgttactt gagttcggg 
      gttgcggga agtaacctg gtttacatc gatccgaac 
      cctgatagt tccctgaga caggatcga cacgagcat 
      ttgactcag ttttttaat gatagtgct gtggagacg 
      gcagtactt gacgagatc ctttaccaa gggattacc 
      atctatcag gattttaat ttcgttgag tggcatcta 
      gattccacc aaaaacttc ataacgtcg gcgaaattc 
      gaatccaag tataagttt gtggaaatt gagctttcg 
      gcgcttttt atctaccat tcgaaaggt gtgagcgct 
      cgcacgttc caagctatc gcccgcagt gggcttgtg 
      tttaatggg cgtttggtc attgataag gattatcaa 
      accaatgat ccttccatc tatggtgcc ggacccttg 
      accatgtac tcgaagaag tactacgcg gaacatttg 
      agtcacaag tactacaat ccaagggag atcggatat 
      aatctaggt tcagcgatt cggaagaag ttagtaccg 
      gagcagttc tgggatccc ccggtcaag gaaacattg 
      attcatcag ccggtggtt caaatttgc aaattcccc 
      ggaaacttg tgctatttg aacgtgttt aaagctggt 
      cggccggta ccactagat gtggccacc aacaagtgc 
      aattatggc cgtgttttg aaaaccggc gattgtaag 
      aagttggat aagcaaggg tacttccgc ctgcacttg 
      aaccagttt caaaccgtc gaaaccatc acctgtttg 
      gccaaattt gacttgaaa gtgcacaat ttgatcaat 
      ttgtggggc aaacacgag atactcctt aatcatctc 
      ttgttgcgg tacgatttg gggatgatc cccgatatg 
      ttccagttt ttcgacgag ccttgggct tgtgctatt 
      tattacaga gactttcca agggtcatt gacgaaatc 
      tctgagttg ttactgacg gcggaaacc aacgaaggg 
      gtgaaacta gtcgatgaa gtccgcgct cagttggag 
      aaaaatgac tggaacccg ttgacgaaa atacaaacc 
      gacgaaatt gaagaaatg tgtcgggat acaagcttg 
      attcctttg attgagaga aaggttttg gactttttg 
      cacgataat ttcatgtat ttgcccatg tatgtgcac 
      cagttgttg atcaggtgg attttgcat caaattcaa 
      acgagtccc ttgttcgtc aaaacgacg ttcatttca 
      aacactagg aagagtttt tcgccgggg attcccttc 
      ctgatgcgg cccacacag tcgaggatg gaataa
    mRNA sequence
    • >TC014955 RA
      atcaaaaga ttttagttt atttattta gataaattc 
      acaacttgt ctgtgttat gccagattt aagttttat 
      tagacaaat aaaatggtg ccaaattaa ggctagcaa 
      aaaatcacc ataaacaaa caactactg tcgacctta 
      acattcctt tacgatttt tgcgcaaaa taaaatact 
      cacatctga agtcttctt cgcctgaaa caggtagca 
      ataaagcag ggaatcttg atcgtccct catcagata 
      gagaaacgt ggttggatc ttggtcggc ttgtgaaaa 
      gcgcgtcac ttgccagat tttgtgcga aaattgcaa 
      ctcaaaatc tggattgta cgcttgtag aagcccatc 
      ttcgattcc aagactcaa ctgcaagaa aatgcacag 
      tttactgac ttttgtcct atcgcccga taatgtgag 
      acgtcttgg accacaaaa cgccaaaga agtggaaaa 
      attgacatt gaaaaatac cgagtattt gttcgggga 
      cacgccggt ggacaaaca acttaagcg gaattatgt 
      ctccttggg ggtttacaa cgacttgga ggagttggc 
      tggagtgat atgttttaa ttgttttcc caacatccc 
      ggcgctacc gccatggga gtgggccta ttggctgca 
      caacaagta ccacattga taacatgac acccatgac 
      cacgctgtg gatacacta cgcggtttg ggaccgcag 
      gtacaccac actgttctt caaacccat cctgcattc 
      actcttcct gacctacga acccatcga caccgtctg 
      tatggtctg tccgcccgg aatccagcg tctggactt 
      cattgatga gtacaccac aagggtact accatatgg 
      tttcacaaa cccatcaaa agtccagac catctacgt 
      agcaaaaaa agtcgattt tgtggctca ctacgtcat 
      acggcgcgc tgttgaaga agacaacga cgacctcgt 
      ggccattat cccaaaaat catcgaaga gacctatgg 
      ccagtacta catttccga gcttttgag caacttgga 
      cgagtctgg acgccagat cattgtggc ggagcaccg 
      agggtgcgc tgtaggtgt cttgtgttt gaacgaaga 
      ggtcaacta tgacctcct gaacgaaga gtttgagtt 
      ggtaccgtt caacggttt gaggaaaca cgatgctga 
      cgatgaagt actcaaaac gcaagtctt gagcgcgac 
      tgcaatccc agttttgga agatagtac cgagcgttt 
      gtccaatac cagcctcca ggaggagac gcgcgaaga 
      gtcagctgc ggagcaaga cgaccaaga caacgtctc 
      cgacgatga gttttctct agtcaactt gtcgtcgtc 
      ccttatttt caatttctt gtacgaaga ggacgaaac 
      tagtgatca cttttttat gtcggagag tgaaactag 
      tatcgaaag ccacttcag tgctgttgt tctgaacga 
      agagtacga ggatcattt gtataacgc tgatcggag 
      tgggggtgg gggtgatgt cgaagggga tgaaaaacg 
      catccctcg acggaaaga atccaaact ggataatat 
      cacaatccc gacgtatta tggcgaacc gaatgcttt 
      cgctcttga aatttgtac agcgatgga tgatcatga 
      gaactgtct ttatttact attccaggc ggcgtttga 
      gtgttttcc agataggga gtatctgat tgtttcgtt 
      accgaccga gacacagat gtgttattt gaagaaaca 
      ctttgcaag ggtcacacc gagacccaa ctcggtgtt 
      taatcacga gttgtatgt tttgcacaa gagtgcgat 
      tttggggac tttgtgggc aagagtggc agtttcgga 
      ggataagcc tttggttag tgaattggt ggcgacttt 
      gagtaagcc atcaattgt ggaagattt attcaagga 
      agcggtgga tagtaagat ggggcagtt taaggccta 
      tacgttttt ctgcgaaga gcagatgat cgggtgtgc 
      gatcgtcag cgaagaaga agaacacga ataccttga 
      actccacta caacgtcga tccttggct aagccccaa 
      agaaacacg ttttgggac ctacggctc tttagaaca 
      attcctcct atctccaat ttttcaacg ccatatcaa 
      cttcttcac tcgcgaatt gcaccgttt gagcgactt 
      tgacgtcct gttttaccg actcaaacg ctacgacta 
      catctacag tcacaggag cctccctat cgtcaacgc 
      catcataaa catgatccc cgtactccc gaatcgagt 
      ccccgaata cagcaaaca aataaccga cgaatttct 
      ccttcccac gtgtttgca aaaacacca ccaaccgtt 
      cggactcta cctatcatc acttcgcct aagcagcat 
      gcccaagat cgaaatcaa ccaaaaaat ggtcattat 
      aggtgcagg acttacaac aatttcgtt tctcgaatc 
      gttgatttt tggtcgtaa ccccaatta tttgatcgt 
      tttcacgaa cataacagt agtgagtcc acacggggt 
      gacctacca taagcctcc aagccaggt cagggagat 
      ggtttttgt gaacgatgg tcacgtcga tcgccgata 
      tatcgatgc aatttcgtt aaaaaccta cgtccatgt 
      tgtgacagg tgttatgac ttcaattaa ccgcaagga 
      aaagtacgt gggtttgaa cgacgagtc gtaccttcc 
      gtacgatta tctcttcct gatgtgcgg tgagcagtt 
      ccagaaacc accactcag gaagaaaga agtcgtgaa 
      gaagcgaac agagttccc gcagaacgt cttcataat 
      taacacaga agtggacgc tgggaacgc cttcaatgc 
      gctgaaaag cctaacttc ggggcaaaa gcagggcaa 
      tatggtggt ttttggtca ttatctgga agcttgttg 
      ttgtttggc tgcgttact tgagttcgg ggttgcggg 
      aagtaacct ggtttacat cgatccgaa ccctgatag 
      ttccctgag acaggatcg acacgagca tttgactca 
      gttttttaa tgatagtgc tgtggagac ggcagtact 
      tgacgagat cctttacca agggattac catctatca 
      ggattttaa tttcgttga gtggcatct agattccac 
      caaaaactt cataacgtc ggcgaaatt cgaatccaa 
      gtataagtt tgtggaaat tgagctttc ggcgctttt 
      tatctacca ttcgaaagg tgtgagcgc tcgcacgtt 
      ccaagctat cgcccgcag tgggcttgt gtttaatgg 
      gcgtttggt cattgataa ggattatca aaccaatga 
      tccttccat ctatggtgc cggaccctt gaccatgta 
      ctcgaagaa gtactacgc ggaacattt gagtcacaa 
      gtactacaa tccaaggga gatcggata taatctagg 
      ttcagcgat tcggaagaa gttagtacc ggagcagtt 
      ctgggatcc cccggtcaa ggaaacatt gattcatca 
      gccggtggt tcaaatttg caaattccc cggaaactt 
      gtgctattt gaacgtgtt taaagctgg tcggccggt 
      accactaga tgtggccac caacaagtg caattatgg 
      ccgtgtttt gaaaaccgg cgattgtaa gaagttgga 
      taagcaagg gtacttccg cctgcactt gaaccagtt 
      tcaaaccgt cgaaaccat cacctgttt ggccaaatt 
      tgacttgaa agtgcacaa tttgatcaa tttgtgggg 
      caaacacga gatactcct taatcatct cttgttgcg 
      gtacgattt ggggatgat ccccgatat gttccagtt 
      tttcgacga gccttgggc ttgtgctat ttattacag 
      agactttcc aagggtcat tgacgaaat ctctgagtt 
      gttactgac ggcggaaac caacgaagg ggtgaaact 
      agtcgatga agtccgcgc tcagttgga gaaaaatga 
      ctggaaccc gttgacgaa aatacaaac cgacgaaat 
      tgaagaaat gtgtcggga tacaagctt gattccttt 
      gattgagag aaaggtttt ggacttttt gcacgataa 
      tttcatgta tttgcccat gtatgtgca ccagttgtt 
      gatcaggtg gattttgca tcaaattca aacgagtcc 
      cttgttcgt caaaacgac gttcatttc aaacactag 
      gaagagttt ttcgccggg gattccctt cctgatgcg 
      gcccacaca gtcgaggat ggaataaaa tatgataca 
      gtagagaat tcggttttt tattgggaa accaagaga 
      attcaatgg gtgtcagat cagggtaaa aggagaaga 
      aaataattg gaacgcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY