General information | |||
TC014927 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 8008255..8009601 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC014927 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05724 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014927 RA
MKTSIIFLL SLASALAHP LLSCSCGVA PMPCNCGLG SGEDVCHDS DKPKTCTPL VVRPLEVPK PKLPAPLLL PPVGSPCSC HTSLVKPAV VPNPPCTAC SGGYAGSID VLNNQHLTL SQQQQILFG GGCSGAQNG GLYGAQAGA YGYTPVARP ILVDDIEKT PVNPANYVP ADPVSLSVA YKMAQEKCH HNEDKLSFG FRSLPKDVP IYVRRMGNN IPEENLFNL NGQIVELKK IACQSRKSL DDEAAEQLA DLQEEEEEA RMAVNPYKS LTLGELGYG PAKIEGQFV DLPSAGPGP SFGEGAASK NCDDGFKPG NVIITQVPK VENLAMPPP LPEPCPADE EAFIPPPPA VPQGALFTS PYTYLSSAS SSSASTKNL YSKSVFLQK VGCS
- >TC014927 RA
atgaaaacc tcaatcatc tttctacta tctctcgcc tcggcactg gcccacccc ctcctgtcc tgctcgtgt ggggtcgcc cccatgccc tgcaactgc ggcctgggc tccggcgaa gacgtctgc cacgacagc gacaagccc aaaacctgc acccccttg gtcgttcgc cccttggaa gtccccaaa cccaaacta cccgcccct ttgctactg cccccagtt ggcagccct tgcagttgc cacacctcg cttgtcaaa ccggcagtg gtgccaaac cccccgtgc acggcctgc tccggagga tacgccggg agtatcgac gttttgaac aaccagcac ttgaccctc agccaacag cagcagatt ctgttcggg ggcggctgc agtggggcc caaaatggg ggcctgtat ggggcccaa gcgggcgct tatggctac accccggtc gcgagaccc atccttgtt gatgatatc gagaaaact ccggtgaat cccgcaaat tacgtgcct gctgatccc gtttccctg tcagttgcg tataaaatg gcccaggaa aagtgccac cataacgaa gataagctc tctttcggg ttcaggagt ttgccgaag gacgtcccg atttacgtg aggcgaatg ggaaataac atccccgaa gaaaatttg ttcaatttg aacggacaa atcgtcgaa ttgaagaaa atcgcgtgc cagtctcgc aagtcgctc gatgacgaa gccgccgag cagctggcc gacctccag gaggaggag gaggaggcc aggatggcc gtcaaccct tacaagtca ctcactttg ggcgaactc ggttacgga cctgccaag atcgagggc cagtttgtg gatctcccg tcggccggt ccaggtccc tcgtttggc gaaggcgca gctagtaag aactgtgat gatggtttc aagcccggg aatgtgatc ataacccag gtgcccaaa gttgaaaat ctggcgatg ccgccccca cttccggag ccatgtccg gctgatgag gaggcgttt attccgccg cctccagct gtgccacaa ggggcgctc ttcactagc ccttacacg tatttgtcg agtgcgtcg tcctcgtcg gcctccact aaaaatctc tactccaag agcgtgttc ttgcaaaag gttgggtgc agttaa
- >TC014927 RA
caatccact ccaccattc aacaatgaa aacctcaat catctttct actatctct cgcctcggc actggccca ccccctcct gtcctgctc gtgtggggt cgcccccat gccctgcaa ctgcggcct gggctccgg cgaagacgt ctgccacga cagcgacaa gcccaaaac ctgcacccc cttggtcgt tcgcccctt ggaagtccc caaacccaa actacccgc ccctttgct actgccccc agttggcag cccttgcag ttgccacac ctcgcttgt caaaccggc agtggtgcc aaacccccc gtgcacggc ctgctccgg aggatacgc cgggagtat cgacgtttt gaacaacca gcacttgac cctcagcca acagcagca gattctgtt cgggggcgg ctgcagtgg ggcccaaaa tgggggcct gtatggggc ccaagcggg cgcttatgg ctacacccc ggtcgcgag acccatcct tgttgatga tatcgagaa aactccggt gaatcccgc aaattacgt gcctgctga tcccgtttc cctgtcagt tgcgtataa aatggccca ggaaaagtg ccaccataa cgaagataa gctctcttt cgggttcag gagtttgcc gaaggacgt cccgattta cgtgaggcg aatgggaaa taacatccc cgaagaaaa tttgttcaa tttgaacgg acaaatcgt cgaattgaa gaaaatcgc gtgccagtc tcgcaagtc gctcgatga cgaagccgc cgagcagct ggccgacct ccaggagga ggaggagga ggccaggat ggccgtcaa cccttacaa gtcactcac tttgggcga actcggtta cggacctgc caagatcga gggccagtt tgtggatct cccgtcggc cggtccagg tccctcgtt tggcgaagg cgcagctag taagaactg tgatgatgg tttcaagcc cgggaatgt gatcataac ccaggtgcc caaagttga aaatctggc gatgccgcc cccacttcc ggagccatg tccggctga tgaggaggc gtttattcc gccgcctcc agctgtgcc acaaggggc gctcttcac tagccctta cacgtattt gtcgagtgc gtcgtcctc gtcggcctc cactaaaaa tctctactc caagagcgt gttcttgca aaaggttgg gtgcagtta agctgtgat tggttgttt cgatggttt tgattgaat aaagaaaat tgataaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
