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Gene information
General information
TC014927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8008255..8009601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014927
    iBeetle screen iB_05724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014927 RA
      MKTSIIFLL SLASALAHP LLSCSCGVA PMPCNCGLG 
      SGEDVCHDS DKPKTCTPL VVRPLEVPK PKLPAPLLL 
      PPVGSPCSC HTSLVKPAV VPNPPCTAC SGGYAGSID 
      VLNNQHLTL SQQQQILFG GGCSGAQNG GLYGAQAGA 
      YGYTPVARP ILVDDIEKT PVNPANYVP ADPVSLSVA 
      YKMAQEKCH HNEDKLSFG FRSLPKDVP IYVRRMGNN 
      IPEENLFNL NGQIVELKK IACQSRKSL DDEAAEQLA 
      DLQEEEEEA RMAVNPYKS LTLGELGYG PAKIEGQFV 
      DLPSAGPGP SFGEGAASK NCDDGFKPG NVIITQVPK 
      VENLAMPPP LPEPCPADE EAFIPPPPA VPQGALFTS 
      PYTYLSSAS SSSASTKNL YSKSVFLQK VGCS
    CDS sequence
    • >TC014927 RA
      atgaaaacc tcaatcatc tttctacta tctctcgcc 
      tcggcactg gcccacccc ctcctgtcc tgctcgtgt 
      ggggtcgcc cccatgccc tgcaactgc ggcctgggc 
      tccggcgaa gacgtctgc cacgacagc gacaagccc 
      aaaacctgc acccccttg gtcgttcgc cccttggaa 
      gtccccaaa cccaaacta cccgcccct ttgctactg 
      cccccagtt ggcagccct tgcagttgc cacacctcg 
      cttgtcaaa ccggcagtg gtgccaaac cccccgtgc 
      acggcctgc tccggagga tacgccggg agtatcgac 
      gttttgaac aaccagcac ttgaccctc agccaacag 
      cagcagatt ctgttcggg ggcggctgc agtggggcc 
      caaaatggg ggcctgtat ggggcccaa gcgggcgct 
      tatggctac accccggtc gcgagaccc atccttgtt 
      gatgatatc gagaaaact ccggtgaat cccgcaaat 
      tacgtgcct gctgatccc gtttccctg tcagttgcg 
      tataaaatg gcccaggaa aagtgccac cataacgaa 
      gataagctc tctttcggg ttcaggagt ttgccgaag 
      gacgtcccg atttacgtg aggcgaatg ggaaataac 
      atccccgaa gaaaatttg ttcaatttg aacggacaa 
      atcgtcgaa ttgaagaaa atcgcgtgc cagtctcgc 
      aagtcgctc gatgacgaa gccgccgag cagctggcc 
      gacctccag gaggaggag gaggaggcc aggatggcc 
      gtcaaccct tacaagtca ctcactttg ggcgaactc 
      ggttacgga cctgccaag atcgagggc cagtttgtg 
      gatctcccg tcggccggt ccaggtccc tcgtttggc 
      gaaggcgca gctagtaag aactgtgat gatggtttc 
      aagcccggg aatgtgatc ataacccag gtgcccaaa 
      gttgaaaat ctggcgatg ccgccccca cttccggag 
      ccatgtccg gctgatgag gaggcgttt attccgccg 
      cctccagct gtgccacaa ggggcgctc ttcactagc 
      ccttacacg tatttgtcg agtgcgtcg tcctcgtcg 
      gcctccact aaaaatctc tactccaag agcgtgttc 
      ttgcaaaag gttgggtgc agttaa
    mRNA sequence
    • >TC014927 RA
      caatccact ccaccattc aacaatgaa aacctcaat 
      catctttct actatctct cgcctcggc actggccca 
      ccccctcct gtcctgctc gtgtggggt cgcccccat 
      gccctgcaa ctgcggcct gggctccgg cgaagacgt 
      ctgccacga cagcgacaa gcccaaaac ctgcacccc 
      cttggtcgt tcgcccctt ggaagtccc caaacccaa 
      actacccgc ccctttgct actgccccc agttggcag 
      cccttgcag ttgccacac ctcgcttgt caaaccggc 
      agtggtgcc aaacccccc gtgcacggc ctgctccgg 
      aggatacgc cgggagtat cgacgtttt gaacaacca 
      gcacttgac cctcagcca acagcagca gattctgtt 
      cgggggcgg ctgcagtgg ggcccaaaa tgggggcct 
      gtatggggc ccaagcggg cgcttatgg ctacacccc 
      ggtcgcgag acccatcct tgttgatga tatcgagaa 
      aactccggt gaatcccgc aaattacgt gcctgctga 
      tcccgtttc cctgtcagt tgcgtataa aatggccca 
      ggaaaagtg ccaccataa cgaagataa gctctcttt 
      cgggttcag gagtttgcc gaaggacgt cccgattta 
      cgtgaggcg aatgggaaa taacatccc cgaagaaaa 
      tttgttcaa tttgaacgg acaaatcgt cgaattgaa 
      gaaaatcgc gtgccagtc tcgcaagtc gctcgatga 
      cgaagccgc cgagcagct ggccgacct ccaggagga 
      ggaggagga ggccaggat ggccgtcaa cccttacaa 
      gtcactcac tttgggcga actcggtta cggacctgc 
      caagatcga gggccagtt tgtggatct cccgtcggc 
      cggtccagg tccctcgtt tggcgaagg cgcagctag 
      taagaactg tgatgatgg tttcaagcc cgggaatgt 
      gatcataac ccaggtgcc caaagttga aaatctggc 
      gatgccgcc cccacttcc ggagccatg tccggctga 
      tgaggaggc gtttattcc gccgcctcc agctgtgcc 
      acaaggggc gctcttcac tagccctta cacgtattt 
      gtcgagtgc gtcgtcctc gtcggcctc cactaaaaa 
      tctctactc caagagcgt gttcttgca aaaggttgg 
      gtgcagtta agctgtgat tggttgttt cgatggttt 
      tgattgaat aaagaaaat tgataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY