Status
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Gene information
General information
TC033446
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014923,TC014924
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8017795..8032551
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033446
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05723

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033446 RA
      MGENVENTP GRQAIAVMK DIQKEIIFK KPVGTPRKR 
      VKEKVLDED TYVEHIGKI IQRDFFPDL EKLKAQNEY 
      LEAVERNDV TKMRQLYMK YSGNKPPTQ RIPSPATFE 
      TPVHNSFET EAPPKPPPE KSEPKLSLD QFLNSHTSE 
      DNRSFSEIL AESEKKHQE KYSFLYKEE GNSEKERQE 
      QLVLPSIVK QGELPEKKL NVDTWSYKN KNYIMYIPD 
      GVPLTQEEE MELNKNRQE VVHCNTRLT INPFNEIQS 
      KEAITELVK NQAKIHDGK IGVDGKELM KDSPQIRGF 
      SFVKTPSPH PNLTDTPLM TWGEIEGTP FRLNGSDTP 
      LPHSQGPSF KIMEIDEGE SSSSGPLSR FNSLFCFTS 
      PAVKKLLGW KQGDEEEKW AEKAVDSLV KKLKKRKGA 
      IEELERALS CPGTQSNCV TIPRSLDGR LQVSHRKGL 
      PHVIYCRVW RWPDLQSHH ELKPLEHCK FPFWAKQKE 
      VCINPYHYK RVESQVLPP VLVPRHSDF VPGHSLLPF 
      QQVVEPNMP QNISYNSNG FNSNHLSPS SPLSSVSSP 
      RSVSSNNPQ SPYSNLAET PPPAYSPTD ENTNNNTSP 
      QPSLNTDVQ PVAYQEQPY WASIAYYEL NFRVGEVFH 
      CHSTSVIVD GFTNPSNNS DRFCLGQLS NVNRNSTIE 
      NTRRHIGKG VHLYYVNGE VYAECLSDS PIFVQSRNC 
      NHHHGFHPS AVCKIPAGC SLRIFNNAE FAQLLSQCV 
      NHGFEAVYE LTKMCMIRM SFVKGWGAE YHRQDVTST 
      PCWIEVHLN GPLQWLDKV LTQMGSPHN AISSVS
    CDS sequence
    • >TC033446 RA
      atgggtgaa aacgtggaa aatacaccc gggcggcag 
      gcaattgcc gtgatgaaa gacatccag aaggaaatt 
      attttcaaa aaaccggtg ggaacgcct cggaaacga 
      gtcaaagag aaggtttta gatgaagac acttatgtg 
      gagcacatt ggtaagatt atccaacgc gactttttc 
      cccgatctt gaaaaactc aaagcccag aacgaatat 
      ttggaagcc gtcgagaga aacgatgtg accaaaatg 
      aggcaactt tatatgaaa tatagcggg aataaaccg 
      ccaacacag aggatacca agccctgcg acttttgag 
      acacctgtc cacaattcg tttgagact gaagccccg 
      ccaaagcca ccccctgag aaaagcgaa ccgaaattg 
      agtttggat cagtttttg aattcgcac acgtctgag 
      gacaatcga agttttagt gagattttg gccgaatct 
      gagaaaaag caccaggag aagtattcg tttttatac 
      aaggaggag ggtaacagt gagaaggag cgacaggaa 
      cagttggtt ttgccctca attgtgaaa cagggggaa 
      ttaccggag aaaaaacta aacgtggac acttggagc 
      tacaaaaat aaaaactac ataatgtac attcctgat 
      ggggttccc ctgacacaa gaagaagaa atggaattg 
      aacaaaaac cggcaagaa gtcgtccac tgtaacacc 
      cgcctcacc atcaaccct ttcaacgaa atccaaagc 
      aaggaggct atcaccgaa ctggtcaaa aaccaggcg 
      aaaatccac gacggtaaa atcggcgtt gacggcaaa 
      gaactgatg aaagacagc ccccagatc aggggcttc 
      tccttcgtg aagaccccc tcgccccac ccgaacctc 
      accgacacc cccttgatg acgtggggc gaaatcgag 
      ggaaccccc tttcgactg aacggcagc gatacgccc 
      ctgccccat tcgcaaggc ccctcgttt aaaatcatg 
      gagatcgac gagggggag tcctccagc agcggcccc 
      ttgtccaga ttcaacagc ctcttctgc ttcaccagc 
      cccgcggtg aagaagctg ctcggctgg aagcagggc 
      gacgaggag gagaagtgg gcagagaag gcagtggac 
      agcctcgtc aagaagctg aagaagcgc aagggggcc 
      attgaggag ctcgagcgc gcgctttcc tgtcccggg 
      acgcagagc aactgcgtg acgataccc cgcagcctc 
      gacggccgc ctccaggtg tcccaccgc aaaggcctc 
      ccccacgtg atctactgc cgggtctgg cgctggccc 
      gacctgcag agccaccac gagctgaaa cctctggag 
      cactgcaag tttccgttt tgggccaag cagaaggag 
      gtctgcatc aacccctac cactacaaa agagtggaa 
      agtcaagtt ttgccgccg gtcttggtc ccccgacac 
      agcgatttt gtcccaggg cactcgctc ctaccattt 
      cagcaagtg gtggagccc aacatgccc cagaatatt 
      agttataat tccaatgga tttaattcc aatcatttg 
      agtccgagt tcgccactg tcgagtgtg tctagtccg 
      aggagtgtt tcgtcgaat aatccgcag agtccgtat 
      tcgaatttg gcggagact ccgcccccg gcctatagc 
      cccaccgac gagaatacg aataataat acgagtccg 
      caaccgtcg cttaataca gatgtgcaa ccggtggcc 
      tatcaggag cagccctat tgggcttct atcgcctac 
      tatgagttg aattttcgc gtcggggaa gttttccat 
      tgccattcg acgtccgtt attgttgat gggttcacc 
      aatcccagc aacaacagc gacagattt tgcttaggg 
      caactaagc aacgtaaac cgcaactca accatcgaa 
      aacacccgc cgtcacatc ggcaaaggg gtccaccta 
      tactacgtc aacggcgaa gtttacgcc gaatgtctc 
      tccgattcg ccaattttc gtccaatcc cgcaactgt 
      aaccaccac catgggttt cacccatcc gccgtgtgt 
      aaaatcccg gctggttgt tcgttgcgt attttcaac 
      aacgccgaa tttgctcaa ctcctctcc caatgcgtg 
      aatcatggt tttgaggcc gtttacgaa ctcacaaaa 
      atgtgtatg atacgtatg agcttcgtc aagggttgg 
      ggcgcggaa taccatcgt caggacgtc acaagcacc 
      ccctgctgg atcgaggtg catctcaac ggccccctc 
      cagtggctc gacaaggtg ctgacccaa atggggtcc 
      cctcacaat gccataagt tcggtctcg taa
    mRNA sequence
    • >TC033446 RA
      cgaattttg aaagtgaca gtttgttgt gacgtttcg 
      ttgacagct aacctaaaa gttatcaca gtgtggaaa 
      atcacttaa aaatgggtg aaaacgtgg aaaatacac 
      ccgggcggc aggcaattg ccgtgatga aagacatcc 
      agaaggaaa ttattttca aaaaaccgg tgggaacgc 
      ctcggaaac gagtcaaag agaaggttt tagatgaag 
      acacttatg tggagcaca ttggtaaga ttatccaac 
      gcgactttt tccccgatc ttgaaaaac tcaaagccc 
      agaacgaat atttggaag ccgtcgaga gaaacgatg 
      tgaccaaaa tgaggcaac tttatatga aatatagcg 
      ggaataaac cgccaacac agaggatac caagccctg 
      cgacttttg agacacctg tccacaatt cgtttgaga 
      ctgaagccc cgccaaagc caccccctg agaaaagcg 
      aaccgaaat tgagtttgg atcagtttt tgaattcgc 
      acacgtctg aggacaatc gaagtttta gtgagattt 
      tggccgaat ctgagaaaa agcaccagg agaagtatt 
      cgtttttat acaaggagg agggtaaca gtgagaagg 
      agcgacagg aacagttgg ttttgccct caattgtga 
      aacaggggg aattaccgg agaaaaaac taaacgtgg 
      acacttgga gctacaaaa ataaaaact acataatgt 
      acattcctg atggggttc ccctgacac aagaagaag 
      aaatggaat tgaacaaaa accggcaag aagtcgtcc 
      actgtaaca cccgcctca ccatcaacc ctttcaacg 
      aaatccaaa gcaaggagg ctatcaccg aactggtca 
      aaaaccagg cgaaaatcc acgacggta aaatcggcg 
      ttgacggca aagaactga tgaaagaca gcccccaga 
      tcaggggct tctccttcg tgaagaccc cctcgcccc 
      acccgaacc tcaccgaca cccccttga tgacgtggg 
      gcgaaatcg agggaaccc cctttcgac tgaacggca 
      gcgatacgc ccctgcccc attcgcaag gcccctcgt 
      ttaaaatca tggagatcg acgaggggg agtcctcca 
      gcagcggcc ccttgtcca gattcaaca gcctcttct 
      gcttcacca gccccgcgg tgaagaagc tgctcggct 
      ggaagcagg gcgacgagg aggagaagt gggcagaga 
      aggcagtgg acagcctcg tcaagaagc tgaagaagc 
      gcaaggggg ccattgagg agctcgagc gcgcgcttt 
      cctgtcccg ggacgcaga gcaactgcg tgacgatac 
      cccgcagcc tcgacggcc gcctccagg tgtcccacc 
      gcaaaggcc tcccccacg tgatctact gccgggtct 
      ggcgctggc ccgacctgc agagccacc acgagctga 
      aacctctgg agcactgca agtttccgt tttgggcca 
      agcagaagg aggtctgca tcaacccct accactaca 
      aaagagtgg aaagtcaag ttttgccgc cggtcttgg 
      tcccccgac acagcgatt ttgtcccag ggcactcgc 
      tcctaccat ttcagcaag tggtggagc ccaacatgc 
      cccagaata ttagttata attccaatg gatttaatt 
      ccaatcatt tgagtccga gttcgccac tgtcgagtg 
      tgtctagtc cgaggagtg tttcgtcga ataatccgc 
      agagtccgt attcgaatt tggcggaga ctccgcccc 
      cggcctata gccccaccg acgagaata cgaataata 
      atacgagtc cgcaaccgt cgcttaata cagatgtgc 
      aaccggtgg cctatcagg agcagccct attgggctt 
      ctatcgcct actatgagt tgaattttc gcgtcgggg 
      aagttttcc attgccatt cgacgtccg ttattgttg 
      atgggttca ccaatccca gcaacaaca gcgacagat 
      tttgcttag ggcaactaa gcaacgtaa accgcaact 
      caaccatcg aaaacaccc gccgtcaca tcggcaaag 
      gggtccacc tatactacg tcaacggcg aagtttacg 
      ccgaatgtc tctccgatt cgccaattt tcgtccaat 
      cccgcaact gtaaccacc accatgggt ttcacccat 
      ccgccgtgt gtaaaatcc cggctggtt gttcgttgc 
      gtattttca acaacgccg aatttgctc aactcctct 
      cccaatgcg tgaatcatg gttttgagg ccgtttacg 
      aactcacaa aaatgtgta tgatacgta tgagcttcg 
      tcaagggtt ggggcgcgg aataccatc gtcaggacg 
      tcacaagca ccccctgct ggatcgagg tgcatctca 
      acggccccc tccagtggc tcgacaagg tgctgaccc 
      aaatggggt cccctcaca atgccataa gttcggtct 
      cgtaatcgg taataacac gggacgtat gagtgccat 
      ttcaggtta tgcaaatgc aacggtggg aaattacgt 
      gttgcctta cggtaaagt gcatttttc agcatcgac 
      gaatgatta tttatgtgt acaggaaac cgagctgaa 
      aacttgtaa attctggat gtaattata acgaattat 
      taattataa tgtgtctgt cttaattga tctctagat 
      ggctacgat tttattggt ttgttggtt atttacagt 
      ttaattcac agacagcgt ttttaaata ttgtttgac 
      tttatttta ttgttttgg tggggcctt cttgcaaaa 
      aatgacagc attttaagt ttagcaaga atgtgccgc 
      cctgtatat taaaaaaaa attaaaaac tttctcaag 
      catcaatgc aggtcgctg atataccgg gtgatcaag 
      aatgcttgt ttcggttgg caacggtgt gacatttga 
      cgcgataag atttttggt tatgtcctt attgccaac 
      tgaaaaatc ttttttctt aattatttt tcgagctgg 
      tttcgacaa aatttctac gtttgtact taattcctg 
      cagtgatcc tcgaggaag atttgcctt tggctgaac 
      aactggttc ggtggtaca aatacgagt gtaatagaa 
      tcataaaga atattttgt gcaattaat aaactccgt 
      tagccgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY