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Gene information
General information
TC014907 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8813240..8818571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014907
      iBeetle screen iB_05722

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014907 RA
        MSKKNLLLL FDRPQEPVF IGKGSKKTV FDIPNDYLN 
        DKYKPLGVS LSNRFKQDA EETVPVKKI SIPPLGEIL 
        ELERDANFS LFIPKHRRI AGRLIDIFL GMRNVDDLL 
        SVAVYARDR VNPYLFSYA LSVAILHRQ DTQDIDLPS 
        FIESFPDKY VDSKVFAKA REEATVVPE GSRTPIEIP 
        RDYTASDLE EEHRLAYFR EDLGINLHH WHWHLVYPF 
        EAAREVVAK NRRGELFYY MHQQIIARY NFERLCNKL 
        KRATRFNDF NEAIKEAYF PKLDSLVSS RAWPSRVAN 
        QKLRDLNRE VDQIKQDVE DLKRWSDRI YAAIHQGSV 
        TDERGRSIT LTENEGIDI LGNMIESSI LSPNRTYYG 
        DLHNMGHVF ISYIHDPDH RHLESFGVM GDSATAMRD 
        PIFYRWHSY IDDIFQEYK ATLPRYTEN QLNYPGITV 
        SNIEVPSQG SSKNTFNTF WQQSDVDLS RGMDFQPRG 
        SVFVRFTHL QHQPFTYKI TVNNQSNGN RKGTCRIFL 
        APKTDERGN PWLFRDQKI MFIELDKFT VNLKQGQNT 
        ITRASSQSS VTIPFERTF RNLDLNRPQ GGEELAQFN 
        FCGCGWPQH MLIPKGTPE GMKSQLFVM ISNYDDDKI 
        DQSTEGVCN DAGSYCGIK DKLYPDRRS MGYPFDRMP 
        RNGVDTLQQ FLTPNMRVQ DVTIKFSDR TVRPKQRN
      CDS sequence
      • >TC014907 RA
        atgtccaag aagaacctc ctcctgctg ttcgaccgc 
        ccccaggag cccgtcttc atcggcaaa ggctcgaaa 
        aaaaccgtc ttcgacatc cccaacgac tacctcaac 
        gacaaatac aagccgttg ggcgtctcc ctcagcaac 
        cgcttcaag caggacgcc gaggagacc gtccctgtg 
        aagaagatc tccattccg ccacttggt gaaatcctc 
        gaattggaa cgcgatgcc aacttctcg ctgtttatt 
        cccaaacac cggcgcatc gccggccgc ctcatcgac 
        atcttcctg gggatgcgc aacgtggac gacctcctc 
        tccgtggcg gtgtatgcg cgcgaccgc gtcaacccc 
        tacctcttc agctacgcc ctctccgtg gccatcctc 
        caccggcag gacacccag gacatcgac ctgccctcc 
        ttcatcgaa tcgtttcct gataagtac gtcgactcc 
        aaggtgttt gccaaagcg cgtgaagaa gccacggtg 
        gtgcccgaa ggttcgcgt actcccatc gaaatcccg 
        agggattac acagcgtcg gatttggag gaggagcac 
        cgactggcc tacttccgg gaagatctg gggatcaac 
        ctccaccac tggcactgg cacctggtg taccctttc 
        gaagcggcg agggaagtc gtggcgaag aaccgaaga 
        ggggagttg ttttattac atgcaccag cagatcatc 
        gccaggtac aactttgaa cgcttgtgt aataaactg 
        aagagggca acgaggttt aatgatttc aatgaggcg 
        attaaagag gcgtatttt cccaaactg gacagtttg 
        gtgtcaagt cgggcttgg ccttcgcgt gtcgcaaac 
        caaaaactg agggatttg aaccgggag gttgaccag 
        attaagcaa gacgtggaa gacttgaag aggtggagt 
        gaccggatt tatgcagca attcatcaa gggagtgtc 
        actgatgaa cgcggccgc agcatcacc ctcaccgaa 
        aacgaaggc atcgacatc ctcggcaac atgatcgag 
        tccagcatc ctctccccc aaccgcacg tactacggc 
        gacttgcac aacatgggc cacgtcttc atctcgtac 
        atccacgac cccgaccat cgccacttg gagtccttc 
        ggggtcatg ggcgactcc gccacggcc atgcgggac 
        cccatcttc taccgttgg cactcctac atcgacgac 
        atcttccag gagtacaaa gccaccttg cctcgctac 
        accgaaaac cagctcaat taccccgga attaccgtc 
        agtaacatc gaagtcccg tcacaggga agctccaaa 
        aatacgttc aacaccttc tggcaacag tccgacgtt 
        gatttatcg cgcgggatg gacttccaa ccccgcggt 
        tccgtcttt gtccgcttc acacatttg caacaccaa 
        cccttcact tacaaaatc actgttaat aaccagagt 
        aacggaaat aggaaaggc acgtgtcgg attttcctg 
        gcacccaag actgatgaa agggggaat ccctggctg 
        ttccgtgac cagaaaatc atgttcatt gagttggat 
        aaatttaca gttaacttg aaacaaggc caaaacact 
        atcaccagg gcctcaagt cagtcctca gtgacgatt 
        ccgtttgag cgtacgttc cgtaacttg gatttgaat 
        cgtccacaa ggcggggaa gaactggcg cagtttaat 
        ttctgtggt tgcgggtgg ccgcaacat atgcttatt 
        cctaaggga acccccgaa ggaatgaag tcgcaactt 
        ttcgtaatg atttcgaat tatgacgac gataagatc 
        gaccaaagc acggaaggg gtttgtaac gacgctggg 
        agttattgc gggattaag gataagttg tatccggat 
        cggaggtcc atgggttat ccctttgac aggatgccc 
        aggaatgga gttgacacc cttcaacag ttccttact 
        ccgaatatg cgtgtccag gatgtcact atcaagttt 
        agtgatagg accgtgagg ccgaaacaa cgcaattaa
      mRNA sequence
      • >TC014907 RA
        gcaaacacc tccaccatg tccaagaag aacctcctc 
        ctgctgttc gaccgcccc caggagccc gtcttcatc 
        ggcaaaggc tcgaaaaaa accgtcttc gacatcccc 
        aacgactac ctcaacgac aaatacaag ccgttgggc 
        gtctccctc agcaaccgc ttcaagcag gacgccgag 
        gagaccgtc cctgtgaag aagatctcc attccgcca 
        cttggtgaa atcctcgaa ttggaacgc gatgccaac 
        ttctcgctg tttattccc aaacaccgg cgcatcgcc 
        ggccgcctc atcgacatc ttcctgggg atgcgcaac 
        gtggacgac ctcctctcc gtggcggtg tatgcgcgc 
        gaccgcgtc aacccctac ctcttcagc tacgccctc 
        tccgtggcc atcctccac cggcaggac acccaggac 
        atcgacctg ccctccttc atcgaatcg tttcctgat 
        aagtacgtc gactccaag gtgtttgcc aaagcgcgt 
        gaagaagcc acggtggtg cccgaaggt tcgcgtact 
        cccatcgaa atcccgagg gattacaca gcgtcggat 
        ttggaggag gagcaccga ctggcctac ttccgggaa 
        gatctgggg atcaacctc caccactgg cactggcac 
        ctggtgtac cctttcgaa gcggcgagg gaagtcgtg 
        gcgaagaac cgaagaggg gagttgttt tattacatg 
        caccagcag atcatcgcc aggtacaac tttgaacgc 
        ttgtgtaat aaactgaag agggcaacg aggtttaat 
        gatttcaat gaggcgatt aaagaggcg tattttccc 
        aaactggac agtttggtg tcaagtcgg gcttggcct 
        tcgcgtgtc gcaaaccaa aaactgagg gatttgaac 
        cgggaggtt gaccagatt aagcaagac gtggaagac 
        ttgaagagg tggagtgac cggatttat gcagcaatt 
        catcaaggg agtgtcact gatgaacgc ggccgcagc 
        atcaccctc accgaaaac gaaggcatc gacatcctc 
        ggcaacatg atcgagtcc agcatcctc tcccccaac 
        cgcacgtac tacggcgac ttgcacaac atgggccac 
        gtcttcatc tcgtacatc cacgacccc gaccatcgc 
        cacttggag tccttcggg gtcatgggc gactccgcc 
        acggccatg cgggacccc atcttctac cgttggcac 
        tcctacatc gacgacatc ttccaggag tacaaagcc 
        accttgcct cgctacacc gaaaaccag ctcaattac 
        cccggaatt accgtcagt aacatcgaa gtcccgtca 
        cagggaagc tccaaaaat acgttcaac accttctgg 
        caacagtcc gacgttgat ttatcgcgc gggatggac 
        ttccaaccc cgcggttcc gtctttgtc cgcttcaca 
        catttgcaa caccaaccc ttcacttac aaaatcact 
        gttaataac cagagtaac ggaaatagg aaaggcacg 
        tgtcggatt ttcctggca cccaagact gatgaaagg 
        gggaatccc tggctgttc cgtgaccag aaaatcatg 
        ttcattgag ttggataaa tttacagtt aacttgaaa 
        caaggccaa aacactatc accagggcc tcaagtcag 
        tcctcagtg acgattccg tttgagcgt acgttccgt 
        aacttggat ttgaatcgt ccacaaggc ggggaagaa 
        ctggcgcag tttaatttc tgtggttgc gggtggccg 
        caacatatg cttattcct aagggaacc cccgaagga 
        atgaagtcg caacttttc gtaatgatt tcgaattat 
        gacgacgat aagatcgac caaagcacg gaaggggtt 
        tgtaacgac gctgggagt tattgcggg attaaggat 
        aagttgtat ccggatcgg aggtccatg ggttatccc 
        tttgacagg atgcccagg aatggagtt gacaccctt 
        caacagttc cttactccg aatatgcgt gtccaggat 
        gtcactatc aagtttagt gataggacc gtgaggccg 
        aaacaacgc aattaattt actggatta ctggacttg 
        aataaaaat tttaagcat tt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY