Status
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Gene information
General information
TC033460
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014891,TC014890
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9043975..9061920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033460
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05720

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033460 RA
      MTARSILQV FDQYQKARL NFVKSIAEL ALRPANIPI 
      LDEQNILEL LQPLLSDVC KQIQNCATI ALGRIIQND 
      HTMAKKFTQ THLITLLLA ALPKENKHQ KNSILFVLR 
      SVCKYDPQL ANFIVETGA LNAVITCME DFEPSVQES 
      AAWVIGYIA RHNQNLAKK CIEAGAVPL LIMCLQGPH 
      LTLKQISAS ALCDIAKHS IEQAQNIVD AGAIPYLAK 
      NLSNLDEKL KRQVLAALS ACAKHSAEL AEVVIEAEV 
      FPCVFLHMA HACPHVRKN AAGLTRDVV KHTLELTQL 
      IVNTGGIGA LMELLTEDP GEARIPCIT ALGYIAGHS 
      DQLAMSVLG CKAIVLLGA ILNESKDEN VLAVTAWTI 
      GQIGKHSPE HAKAVAAAN LFPRLVQLY ASEETSEDL 
      KFKCKLALK LCLQKCLLV AALEPLLYD APPNILKYV 
      LGQYSKGPW RPPVARRHF ENVATMLRK PASEKLYLN 
      TSNLSVVGI QVHRPRRLR THHHQQRIL LDTSPVSSA 
      SVVPPPRPS VVLGEDVVS RQVRKIRET VQVEDFERL 
      LIGLGGRRD VATETAFGD VYHDFFEVY YDVLKARMA 
      AASIT
    CDS sequence
    • >TC033460 RA
      atgacagcc aggtcaatt ttgcaagtt ttcgaccaa 
      taccaaaaa gcgcgcctt aactttgtt aaatcaatc 
      gctgaatta gcgctacgc cccgccaac atcccaatc 
      ctcgacgag cagaacatc ctagaactc ctgcagccc 
      cttctaagc gatgtgtgc aaacaaatc caaaactgc 
      gccacgatc gccctcggc cgaatcatc caaaacgac 
      cacaccatg gcgaaaaaa ttcacccaa acccacctg 
      atcaccctc ctgttggca gccctgccc aaagaaaac 
      aagcaccag aaaaactcg attcttttc gtcttgcga 
      tcggtttgt aaatatgac ccgcaattg gcgaatttc 
      atcgttgaa acgggagcc ctcaacgcg gttataacc 
      tgtatggag gattttgaa ccgtcggtt caggagtcg 
      gctgcgtgg gttatcggt tacatcgca cgtcataat 
      caaaatttg gccaagaaa tgcatagaa gccggcgct 
      gtgcctttg ctgattatg tgtcttcaa gggccacac 
      ttgactctg aagcagatt tcggcaagt gccttgtgt 
      gatattgcg aaacattcg atagaacag gcgcagaat 
      attgtggat gctggcgca attccgtac ttggccaag 
      aatttgagt aatttagac gagaaattg aaacggcaa 
      gttttggcc gctttgagc gcctgtgcc aaacattca 
      gctgaactg gccgaagtc gtaatcgaa gctgaagtc 
      ttcccttgt gttttcctc cacatggca cacgcgtgc 
      ccccacgtc cggaaaaac gcagccggt ttaacccgc 
      gacgttgtc aaacacact ctcgagctc acacaactt 
      atcgtcaac actggcgga atcggcgct ttgatggag 
      ctcctcacc gaagacccg ggggaagcc cgtatcccc 
      tgcatcacc gccttggga tacattgca ggtcattcc 
      gatcaattg gcaatgtct gtgctcggt tgtaaggcg 
      atagtttta ctcggagcg attttgaac gaatcgaaa 
      gacgaaaat gtgttagcg gtgacggcg tggactatt 
      ggccaaatc ggcaaacat agtccagag catgccaaa 
      gctgtggca gcggcgaac ttgttccct aggttggta 
      caactgtat gcaagcgaa gagacgtcg gaagatctc 
      aaatttaag tgtaaattg gcgttgaag ttgtgtttg 
      cagaagtgt ctcctggtg gcggcgctt gaaccgctg 
      ttgtatgat gcgccccca aatatactc aagtatgtg 
      ctggggcag tatagtaag ggcccctgg cggccgcca 
      gttgcgcga cgccacttt gaaaatgtc gcgacgatg 
      ttgcgaaaa ccagcctct gaaaagctc tacttaaac 
      accagcaac ctctccgtc gtcggcatc caagtccac 
      cgtcctcgc cgtctccgc acccatcac caccagcag 
      cgcattctc ctggacacc agtccggtg agttcagcc 
      agcgttgtc ccacctcca cgtcccagt gtcgtcctc 
      ggcgaggac gtcgtcagt cgccaggtg cgcaaaatt 
      cgcgaaacc gttcaagtg gaggacttt gaaaggctt 
      ctgatcggc ttaggcgga cgtagagac gtcgcgacg 
      gagacggcg ttcggtgac gtctaccac gacttcttc 
      gaggtgtat tacgacgtg ctgaaggcc aggatggcg 
      gcggcctcc atcacttaa
    mRNA sequence
    • >TC033460 RA
      tgttgggca tcctatgtg tattattat tattattat 
      tattattat taaaaattt aaattaaca gtgccacca 
      accgaagat gcgaaattt cctagatta ttgaatcaa 
      gcgcgccat gttgcaaat cacaaaatg acagccagg 
      tcaattttg caagttttc gaccaatac caaaaagcg 
      cgccttaac tttgttaaa tcaatcgct gaattagcg 
      ctacgcccc gccaacatc ccaatcctc gacgagcag 
      aacatccta gaactcctg cagcccctt ctaagcgat 
      gtgtgcaaa caaatccaa aactgcgcc acgatcgcc 
      ctcggccga atcatccaa aacgaccac accatggcg 
      aaaaaattc acccaaacc cacctgatc accctcctg 
      ttggcagcc ctgcccaaa gaaaacaag caccagaaa 
      aactcgatt cttttcgtc ttgcgatcg gtttgtaaa 
      tatgacccg caattggcg aatttcatc gttgaaacg 
      ggagccctc aacgcggtt ataacctgt atggaggat 
      tttgaaccg tcggttcag gagtcggct gcgtgggtt 
      atcggttac atcgcacgt cataatcaa aatttggcc 
      aagaaatgc atagaagcc ggcgctgtg cctttgctg 
      attatgtgt cttcaaggg ccacacttg actctgaag 
      cagatttcg gcaagtgcc ttgtgtgat attgcgaaa 
      cattcgata gaacaggcg cagaatatt gtggatgct 
      ggcgcaatt ccgtacttg gccaagaat ttgagtaat 
      ttagacgag aaattgaaa cggcaagtt ttggccgct 
      ttgagcgcc tgtgccaaa cattcagct gaactggcc 
      gaagtcgta atcgaagct gaagtcttc ccttgtgtt 
      ttcctccac atggcacac gcgtgcccc cacgtccgg 
      aaaaacgca gccggttta acccgcgac gttgtcaaa 
      cacactctc gagctcaca caacttatc gtcaacact 
      ggcggaatc ggcgctttg atggagctc ctcaccgaa 
      gacccgggg gaagcccgt atcccctgc atcaccgcc 
      ttgggatac attgcaggt cattccgat caattggca 
      atgtctgtg ctcggttgt aaggcgata gttttactc 
      ggagcgatt ttgaacgaa tcgaaagac gaaaatgtg 
      ttagcggtg acggcgtgg actattggc caaatcggc 
      aaacatagt ccagagcat gccaaagct gtggcagcg 
      gcgaacttg ttccctagg ttggtacaa ctgtatgca 
      agcgaagag acgtcggaa gatctcaaa tttaagtgt 
      aaattggcg ttgaagttg tgtttgcag aagtgtctc 
      ctggtggcg gcgcttgaa ccgctgttg tatgatgcg 
      cccccaaat atactcaag tatgtgctg gggcagtat 
      agtaagggc ccctggcgg ccgccagtt gcgcgacgc 
      cactttgaa aatgtcgcg acgatgttg cgaaaacca 
      gcctctgaa aagctctac ttaaacacc agcaacctc 
      tccgtcgtc ggcatccaa gtccaccgt cctcgccgt 
      ctccgcacc catcaccac cagcagcgc attctcctg 
      gacaccagt ccggtgagt tcagccagc gttgtccca 
      cctccacgt cccagtgtc gtcctcggc gaggacgtc 
      gtcagtcgc caggtgcgc aaaattcgc gaaaccgtt 
      caagtggag gactttgaa aggcttctg atcggctta 
      ggcggacgt agagacgtc gcgacggag acggcgttc 
      ggtgacgtc taccacgac ttcttcgag gtgtattac 
      gacgtgctg aaggccagg atggcggcg gcctccatc 
      acttaagcc tttacaaca cgtggaaac ttctgtgat 
      atatacagg gtggcgaaa tggattgca aagtgccag 
      tcaatgtca ataaaacgc gcgagctgg acaggaagg 
      ttttaaggg acacgctgt attcggaga ttttccaaa 
      gacacagac ggtcgattt tcgcgctcg gctcgtttt 
      tacgaatgt taaagacga aaaagtcga actataatg 
      ttatcaaac aagacggtg aaagtgagt gatgatttg 
      tagtgcttt aaaccgtcc cttttcgtt ttcttgcac 
      tttcggctt aaatgtgaa tgtgtgtgt caaaaaagt 
      gccttcacc gatcaccag ttagagcct ctagtgttg 
      ttattaacg ccttcaatt atgtactta atcaccgga 
      tatgtctga attttatac gagtgataa ctgatacta 
      tacaccaaa ttcgtttag ttgacaatt ttatactgc 
      cgaacgtag ttgtaaacc ttattgctg acatgtata 
      ataaaattt gatcaggaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY